TY  - JOUR
AU  - Bufan, Billjana
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Prijić, Ivana
AU  - Radosavljević, Tatjana
AU  - Samardžić, Janko
AU  - Radosavljević, Milica
AU  - Janković, Radmila
AU  - Đuretić, Jasmina
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1470
AB  - Aging is closely related to the main aspects of multiple sclerosis (MS). The average age ofthe MS population is increasing and the number of elderly MS patients is expected to increase. Inaddition to neurons,N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronalcells,  such  as  immune  cells.   The  aim  of  this  study  was  to  investigate  the  role  of  NMDARs  inexperimental autoimmune encephalomyelitis (EAE) in young and aged rats.  Memantine, a non-competitive NMDAR antagonist, was administered to young and agedDark Agoutirats from day7 after immunization.  Antagonizing NMDARs had a more favourable effect on clinical disease,reactivation, and apoptosis of CD4+T cells in the target organ of aged EAE rats. The expression ofthe fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent inaged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expressionin brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advancedoxidation protein products in brain tissue were consistent with previous results. Overall, our resultssuggest that NMDARs play a more important role in the pathogenesis of EAE in aged than inyoung rats.
PB  - Basel, Switzerland : MDPI
T2  - Biomedicines
T1  - NMDA Receptor Antagonist Memantine AmelioratesExperimental Autoimmune Encephalomyelitis in Aged Rats
IS  - 4
SP  - 717
VL  - 12
DO  - 10.3390/biomedicines12040717
ER  - 

@article{
author = "Bufan, Billjana and Ćuruvija, Ivana and Blagojević, Veljko and Grujić-Milanović, Jelica and Prijić, Ivana and Radosavljević, Tatjana and Samardžić, Janko and Radosavljević, Milica and Janković, Radmila and Đuretić, Jasmina",
year = "2024",
abstract = "Aging is closely related to the main aspects of multiple sclerosis (MS). The average age ofthe MS population is increasing and the number of elderly MS patients is expected to increase. Inaddition to neurons,N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronalcells,  such  as  immune  cells.   The  aim  of  this  study  was  to  investigate  the  role  of  NMDARs  inexperimental autoimmune encephalomyelitis (EAE) in young and aged rats.  Memantine, a non-competitive NMDAR antagonist, was administered to young and agedDark Agoutirats from day7 after immunization.  Antagonizing NMDARs had a more favourable effect on clinical disease,reactivation, and apoptosis of CD4+T cells in the target organ of aged EAE rats. The expression ofthe fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent inaged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expressionin brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advancedoxidation protein products in brain tissue were consistent with previous results. Overall, our resultssuggest that NMDARs play a more important role in the pathogenesis of EAE in aged than inyoung rats.",
publisher = "Basel, Switzerland : MDPI",
journal = "Biomedicines",
title = "NMDA Receptor Antagonist Memantine AmelioratesExperimental Autoimmune Encephalomyelitis in Aged Rats",
number = "4",
pages = "717",
volume = "12",
doi = "10.3390/biomedicines12040717"
}

Bufan, B., Ćuruvija, I., Blagojević, V., Grujić-Milanović, J., Prijić, I., Radosavljević, T., Samardžić, J., Radosavljević, M., Janković, R.,& Đuretić, J.. (2024). NMDA Receptor Antagonist Memantine AmelioratesExperimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines
Basel, Switzerland : MDPI., 12(4), 717.
https://doi.org/10.3390/biomedicines12040717

Bufan B, Ćuruvija I, Blagojević V, Grujić-Milanović J, Prijić I, Radosavljević T, Samardžić J, Radosavljević M, Janković R, Đuretić J. NMDA Receptor Antagonist Memantine AmelioratesExperimental Autoimmune Encephalomyelitis in Aged Rats. in Biomedicines. 2024;12(4):717.
doi:10.3390/biomedicines12040717 .

Bufan, Billjana, Ćuruvija, Ivana, Blagojević, Veljko, Grujić-Milanović, Jelica, Prijić, Ivana, Radosavljević, Tatjana, Samardžić, Janko, Radosavljević, Milica, Janković, Radmila, Đuretić, Jasmina, "NMDA Receptor Antagonist Memantine AmelioratesExperimental Autoimmune Encephalomyelitis in Aged Rats" in Biomedicines, 12, no. 4 (2024):717,
https://doi.org/10.3390/biomedicines12040717 . .

TY  - JOUR
AU  - Grujić-Milanović, Jelica
AU  - Rajković, Jovana
AU  - Milanović, Slađan
AU  - Jaćević, Vesna
AU  - Miloradović, Zoran
AU  - Nežić, Lana
AU  - Novaković, Radmila
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1386
AB  - Cardiovascular diseases (CVDs) are a group of diseases with a very high rate of morbidity and mortality. The clinical presentation of CVDs can vary from asymptomatic to classic symptoms such as chest pain in patients with myocardial infarction. Current therapeutics for CVDs mainly target disease symptoms. The most common CVDs are coronary artery disease, acute myocardial infarction, atrial fibrillation, chronic heart failure, arterial hypertension, and valvular heart disease. In their treatment, conventional therapies and pharmacological therapies are used. However, the use of herbal medicines in the therapy of these diseases has also been reported in the literature, resulting in a need for critical evaluation of advances related to their use. Therefore, we carried out a narrative review of pharmacological and herbal therapeutic effects reported for these diseases. Data for this comprehensive review were obtained from electronic databases such as MedLine, PubMed, Web of Science, Scopus, and Google Scholar. Conventional therapy requires an individual approach to the patients, as when patients do not respond well, this often causes allergic effects or various other unwanted effects. Nowadays, medicinal plants as therapeutics are frequently used in different parts of the world. Preclinical/clinical pharmacology studies have confirmed that some bioactive compounds may have beneficial therapeutic effects in some common CVDs. The natural products analyzed in this review are promising phytochemicals for adjuvant and complementary drug candidates in CVDs pharmacotherapy, and some of them have already been approved by the FDA. There are insufficient clinical studies to compare the effectiveness of natural products compared to approved therapeutics for the treatment of CVDs. Further long-term studies are needed to accelerate the potential of using natural products for these diseases. Despite this undoubted beneficence on CVDs, there are no strong breakthroughs supporting the implementation of natural products in clinical practice. Nevertheless, they are promising agents in the supplementation and co-therapy of CVDs.
PB  - MDPI (Multidisciplinary Digital Publishing Institute)
T2  - Antioxidants
T2  - Antioxidants
T1  - Natural Substances vs. Approved Drugs in the Treatment of Main Cardiovascular Disorders—Is There a Breakthrough?
IS  - 12
SP  - 2088
VL  - 12
DO  - 10.3390/antiox12122088
ER  - 

@article{
author = "Grujić-Milanović, Jelica and Rajković, Jovana and Milanović, Slađan and Jaćević, Vesna and Miloradović, Zoran and Nežić, Lana and Novaković, Radmila",
year = "2023",
abstract = "Cardiovascular diseases (CVDs) are a group of diseases with a very high rate of morbidity and mortality. The clinical presentation of CVDs can vary from asymptomatic to classic symptoms such as chest pain in patients with myocardial infarction. Current therapeutics for CVDs mainly target disease symptoms. The most common CVDs are coronary artery disease, acute myocardial infarction, atrial fibrillation, chronic heart failure, arterial hypertension, and valvular heart disease. In their treatment, conventional therapies and pharmacological therapies are used. However, the use of herbal medicines in the therapy of these diseases has also been reported in the literature, resulting in a need for critical evaluation of advances related to their use. Therefore, we carried out a narrative review of pharmacological and herbal therapeutic effects reported for these diseases. Data for this comprehensive review were obtained from electronic databases such as MedLine, PubMed, Web of Science, Scopus, and Google Scholar. Conventional therapy requires an individual approach to the patients, as when patients do not respond well, this often causes allergic effects or various other unwanted effects. Nowadays, medicinal plants as therapeutics are frequently used in different parts of the world. Preclinical/clinical pharmacology studies have confirmed that some bioactive compounds may have beneficial therapeutic effects in some common CVDs. The natural products analyzed in this review are promising phytochemicals for adjuvant and complementary drug candidates in CVDs pharmacotherapy, and some of them have already been approved by the FDA. There are insufficient clinical studies to compare the effectiveness of natural products compared to approved therapeutics for the treatment of CVDs. Further long-term studies are needed to accelerate the potential of using natural products for these diseases. Despite this undoubted beneficence on CVDs, there are no strong breakthroughs supporting the implementation of natural products in clinical practice. Nevertheless, they are promising agents in the supplementation and co-therapy of CVDs.",
publisher = "MDPI (Multidisciplinary Digital Publishing Institute)",
journal = "Antioxidants, Antioxidants",
title = "Natural Substances vs. Approved Drugs in the Treatment of Main Cardiovascular Disorders—Is There a Breakthrough?",
number = "12",
pages = "2088",
volume = "12",
doi = "10.3390/antiox12122088"
}

Grujić-Milanović, J., Rajković, J., Milanović, S., Jaćević, V., Miloradović, Z., Nežić, L.,& Novaković, R.. (2023). Natural Substances vs. Approved Drugs in the Treatment of Main Cardiovascular Disorders—Is There a Breakthrough?. in Antioxidants
MDPI (Multidisciplinary Digital Publishing Institute)., 12(12), 2088.
https://doi.org/10.3390/antiox12122088

Grujić-Milanović J, Rajković J, Milanović S, Jaćević V, Miloradović Z, Nežić L, Novaković R. Natural Substances vs. Approved Drugs in the Treatment of Main Cardiovascular Disorders—Is There a Breakthrough?. in Antioxidants. 2023;12(12):2088.
doi:10.3390/antiox12122088 .

Grujić-Milanović, Jelica, Rajković, Jovana, Milanović, Slađan, Jaćević, Vesna, Miloradović, Zoran, Nežić, Lana, Novaković, Radmila, "Natural Substances vs. Approved Drugs in the Treatment of Main Cardiovascular Disorders—Is There a Breakthrough?" in Antioxidants, 12, no. 12 (2023):2088,
https://doi.org/10.3390/antiox12122088 . .

TY  - CONF
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Životić, Maja
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1377
AB  - Prethodne studije su pokazale da klotho poseduje anti-inflamatornu, antifibrotičku i antioksidativnu ulogu, i da postoji direktna pozitivna korelacija izmedu klotho-a i albuminurije kod nekih animalnih modela hronične bubrežne bolesti (CKD). Fokalno segmentna glomeruloskleroza (FSGS) je oblik CKD, koji se odlikuje prisutnom proteinurijom, hipoalbuminemijom i oksidativnim stresom. Ranije smo pokazali da blokada receptora angiotenzina II tipa 1 losartanom (L) smanjuje proteinuriju, glomerulosklerozu, tubulointersticijsku inflamaciju i fibrozu u animalnom modelu FSGS izazvane adriamicinom (ADR, antitumorski lek) kod spontano hipertenzivnih pacova (SHR), ali ove promene u bubrezima su i dalje bile veće od kontrolnih vrednosti. Ekstrakt lista masline (Olea europaea L.) (OLE), dobro poznat kao prirodni antioksidans, bogat je fenolnim jedinjenjima koja se smatraju odgovornim za snažna antioksidativna i anti-inflamatorna dejstva ekstrakta. Stoga smo ispitivali da li tretman losartanom u kombinaciji sa ekstraktom lista masline može biti efikasniji u usporavanju progresije FSGS. Odrasle ženke SHR su podeljene u četiri grupe. Kontrolni pacovi su primili fiziološki rastvor. SHADR, SHADR+L i SHADR+L+OLE grupe su primile ADR (2 mg/kg telesne mase i.v.) dva puta u intervalu od 3 nedelje. Nakon druge injekcije, SHADR+L i SHADR+L+OLE grupe su primale gavažom L (10 mg/kg/dan), odnosno L+OLE (10+80 mg/kg/dan). Na kraju 6-nedeljnog tretmana analizirali smo albuminuriju, nivo azot monoksida (NO,), 6-nitrotriptofan (marker nitracije proteina), klotho i histopatologiju bubrega. U model grupi uočeno je mačajno povećanje albuminurije i indeksa oitećenja bubrega, ito je bilo praćeno značajno smanjenom ekspresijom klotho proteina i 6-nitortriptofana, kao i nivoa NO, u homogenatu bubrega u poredenju sa ovim vrednostima u kontrolnoj grupi. Tretman losartanom je mačajno smanjio albuminuriju, glomerulosklerozu i tubulointersticijska oštećenja u poredenju sa model grupom, ali su oštećenja bubrega i dalje bila mačajno veća nego u kontrolnoj grupi. Takode, vrednost NO, je vraćena na kontrolni nivo, a proteinska ekspresija 6-nitrotriptofana je mačajno povećana, dok je klotho-a ostala nepromenjena, u poredenju sa model grupom. Interesantno, kada je tretmanu losartanom dodat ekstrakt lista masline primećeno je mačajno i još jače smanjenje albuminurije, glomeruloskleroze, tubulointersticijske inflamacije i fibroze u poredenju sa model grupom. Takode, ovi parametri su sniženi do nivoa koji se nisu mačajno razlikovali od onih u kontroli. Pored toga, ekspresija proteina klotho-a u bubrezima je mačajno povećana u poredenju sa grupama SHADR+L i model Naši rezultati su pokazali da je ekstrakt lista masline kombinovan sa losartanom bio efikasniji od samog tretmana losartanom u usporavanju progresije FSGS kod SHR, a ovaj povoljan efekat uključivao je Klotho signalni put.
AB  - Previous studies have shown the anti-inflammatory, antifibrotic and antioxidant properties of klotho, and a direct positive correlation between klotho and albuminuria in some chronic kidney disease (CKD) animal models. Focal segmented glomerulosclerosis (FSGS), a form of CKD, is distinguished by proteinuria, hypoalbuminemia, and oxidative stress. We previously showed that blockade of angiotensin Õ type 1 receptor with losartan (L) decreased proteinuria, glomerulosclerosis, tubulointerstitial inflammation, and fibrosis in animal model of FSGS induced by Adriamycin (ADR, anti-cancer agent) in spontaneously hypertensive rats (SHR), but these renal alterations were still higher than control values. Olive (Olea europaea L.) leaf extract (OLE), well-known as natural antioxidant, is rich in phenolic compounds that are thought to be responsible for its powerful antioxidant and anti-inflammatory properties. Here, we examined if losartan treatment in combination with olive leaf extract consumption could be more effective in slowing down the progression of FSGS. Adult female SHRs were divided in four groups. Control rats received vehicle. SHADR, SHADR+L and SHADR+L+OLE groups received ADR (2 mg/kg body weight i.v.) twice in 3-week-interval. After the second injection, SHADR+L, and SHADR+L+OLE groups received by gavage L (10 mg/kg/day), and L+OLE (10+80 mg/kg/day), respectively. At the end of6-week treatment, we analysed albuminuria, renal nitric oxide level (NO,), 6-nitrotryptophan (marker ofnitration of proteins), klotho, and renal histopathology In model group we found significantly increased albuininuria and renal injury score, followed by significantly decreased klotho protein expressions, NOx level, and 6- nitortryptophan in comparison to these values in control group. Losartan treatment significantly reduced albuminuria, glomerulosclerosis, and tubulointerstitial injuries compared to model group, but renal alterations were still significantly higher than in control group. Furthermore, NO, level reversed to control and 6-nitrotryptophan protein expression significantly increased, but klotho remained unchanged, compared to model group. Interestingly, when olive leaf extract was added to losartan treatment, a significant and even stronger decrease of albuminuria, glomerulosclerosis, tubulointerstitial inflammation and fibrosis were observed in comparison to model group. Also, these parameters were lowered to the levels not significantly different than in control. Furthermore, renal protein expression of klotho was significantly increased compared to SHADR+L and model groups. Our results showed that olive leaf extract in addition to losartan treatment was more effective than losartan alone in slowing down the progression of FSGS in SHR, and that beneficial effect involved the klotho signalling pathway.
PB  - Niš: Punta
C3  - Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book
T1  - Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho
T1  - Ekstrakt lista masline u kombinaciji sa Losartanom ima pozitivan efekat na albuminuriju i progresivnu fokalnu segmentalnu glomerulosklerozu u hipertenzivnih miševa: uloga Klotho
EP  - 159
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1377
ER  - 

@conference{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Grujić-Milanović, Jelica and Životić, Maja and Dekanski, Dragana and Jovović, Đurđica",
year = "2023",
abstract = "Prethodne studije su pokazale da klotho poseduje anti-inflamatornu, antifibrotičku i antioksidativnu ulogu, i da postoji direktna pozitivna korelacija izmedu klotho-a i albuminurije kod nekih animalnih modela hronične bubrežne bolesti (CKD). Fokalno segmentna glomeruloskleroza (FSGS) je oblik CKD, koji se odlikuje prisutnom proteinurijom, hipoalbuminemijom i oksidativnim stresom. Ranije smo pokazali da blokada receptora angiotenzina II tipa 1 losartanom (L) smanjuje proteinuriju, glomerulosklerozu, tubulointersticijsku inflamaciju i fibrozu u animalnom modelu FSGS izazvane adriamicinom (ADR, antitumorski lek) kod spontano hipertenzivnih pacova (SHR), ali ove promene u bubrezima su i dalje bile veće od kontrolnih vrednosti. Ekstrakt lista masline (Olea europaea L.) (OLE), dobro poznat kao prirodni antioksidans, bogat je fenolnim jedinjenjima koja se smatraju odgovornim za snažna antioksidativna i anti-inflamatorna dejstva ekstrakta. Stoga smo ispitivali da li tretman losartanom u kombinaciji sa ekstraktom lista masline može biti efikasniji u usporavanju progresije FSGS. Odrasle ženke SHR su podeljene u četiri grupe. Kontrolni pacovi su primili fiziološki rastvor. SHADR, SHADR+L i SHADR+L+OLE grupe su primile ADR (2 mg/kg telesne mase i.v.) dva puta u intervalu od 3 nedelje. Nakon druge injekcije, SHADR+L i SHADR+L+OLE grupe su primale gavažom L (10 mg/kg/dan), odnosno L+OLE (10+80 mg/kg/dan). Na kraju 6-nedeljnog tretmana analizirali smo albuminuriju, nivo azot monoksida (NO,), 6-nitrotriptofan (marker nitracije proteina), klotho i histopatologiju bubrega. U model grupi uočeno je mačajno povećanje albuminurije i indeksa oitećenja bubrega, ito je bilo praćeno značajno smanjenom ekspresijom klotho proteina i 6-nitortriptofana, kao i nivoa NO, u homogenatu bubrega u poredenju sa ovim vrednostima u kontrolnoj grupi. Tretman losartanom je mačajno smanjio albuminuriju, glomerulosklerozu i tubulointersticijska oštećenja u poredenju sa model grupom, ali su oštećenja bubrega i dalje bila mačajno veća nego u kontrolnoj grupi. Takode, vrednost NO, je vraćena na kontrolni nivo, a proteinska ekspresija 6-nitrotriptofana je mačajno povećana, dok je klotho-a ostala nepromenjena, u poredenju sa model grupom. Interesantno, kada je tretmanu losartanom dodat ekstrakt lista masline primećeno je mačajno i još jače smanjenje albuminurije, glomeruloskleroze, tubulointersticijske inflamacije i fibroze u poredenju sa model grupom. Takode, ovi parametri su sniženi do nivoa koji se nisu mačajno razlikovali od onih u kontroli. Pored toga, ekspresija proteina klotho-a u bubrezima je mačajno povećana u poredenju sa grupama SHADR+L i model Naši rezultati su pokazali da je ekstrakt lista masline kombinovan sa losartanom bio efikasniji od samog tretmana losartanom u usporavanju progresije FSGS kod SHR, a ovaj povoljan efekat uključivao je Klotho signalni put., Previous studies have shown the anti-inflammatory, antifibrotic and antioxidant properties of klotho, and a direct positive correlation between klotho and albuminuria in some chronic kidney disease (CKD) animal models. Focal segmented glomerulosclerosis (FSGS), a form of CKD, is distinguished by proteinuria, hypoalbuminemia, and oxidative stress. We previously showed that blockade of angiotensin Õ type 1 receptor with losartan (L) decreased proteinuria, glomerulosclerosis, tubulointerstitial inflammation, and fibrosis in animal model of FSGS induced by Adriamycin (ADR, anti-cancer agent) in spontaneously hypertensive rats (SHR), but these renal alterations were still higher than control values. Olive (Olea europaea L.) leaf extract (OLE), well-known as natural antioxidant, is rich in phenolic compounds that are thought to be responsible for its powerful antioxidant and anti-inflammatory properties. Here, we examined if losartan treatment in combination with olive leaf extract consumption could be more effective in slowing down the progression of FSGS. Adult female SHRs were divided in four groups. Control rats received vehicle. SHADR, SHADR+L and SHADR+L+OLE groups received ADR (2 mg/kg body weight i.v.) twice in 3-week-interval. After the second injection, SHADR+L, and SHADR+L+OLE groups received by gavage L (10 mg/kg/day), and L+OLE (10+80 mg/kg/day), respectively. At the end of6-week treatment, we analysed albuminuria, renal nitric oxide level (NO,), 6-nitrotryptophan (marker ofnitration of proteins), klotho, and renal histopathology In model group we found significantly increased albuininuria and renal injury score, followed by significantly decreased klotho protein expressions, NOx level, and 6- nitortryptophan in comparison to these values in control group. Losartan treatment significantly reduced albuminuria, glomerulosclerosis, and tubulointerstitial injuries compared to model group, but renal alterations were still significantly higher than in control group. Furthermore, NO, level reversed to control and 6-nitrotryptophan protein expression significantly increased, but klotho remained unchanged, compared to model group. Interestingly, when olive leaf extract was added to losartan treatment, a significant and even stronger decrease of albuminuria, glomerulosclerosis, tubulointerstitial inflammation and fibrosis were observed in comparison to model group. Also, these parameters were lowered to the levels not significantly different than in control. Furthermore, renal protein expression of klotho was significantly increased compared to SHADR+L and model groups. Our results showed that olive leaf extract in addition to losartan treatment was more effective than losartan alone in slowing down the progression of FSGS in SHR, and that beneficial effect involved the klotho signalling pathway.",
publisher = "Niš: Punta",
journal = "Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book",
title = "Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho, Ekstrakt lista masline u kombinaciji sa Losartanom ima pozitivan efekat na albuminuriju i progresivnu fokalnu segmentalnu glomerulosklerozu u hipertenzivnih miševa: uloga Klotho",
pages = "159-153",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1377"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Vajić, U. J., Grujić-Milanović, J., Životić, M., Dekanski, D.,& Jovović, Đ.. (2023). Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book
Niš: Punta., 153-159.
https://hdl.handle.net/21.15107/rcub_rimi_1377

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Vajić UJ, Grujić-Milanović J, Životić M, Dekanski D, Jovović Đ. Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book. 2023;:153-159.
https://hdl.handle.net/21.15107/rcub_rimi_1377 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Grujić-Milanović, Jelica, Životić, Maja, Dekanski, Dragana, Jovović, Đurđica, "Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho" in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book (2023):153-159,
https://hdl.handle.net/21.15107/rcub_rimi_1377 .

TY  - CONF
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Vajić, Una Jovana
AU  - Miloradović, Zoran
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1433
PB  - Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije
C3  - Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
T1  - Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima
EP  - 76
IS  - 1 (supplement 1)
SP  - 75
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1433
ER  - 

@conference{
author = "Mihailović-Stanojević, Nevena and Karanović, Danijela and Grujić-Milanović, Jelica and Ivanov, Milan and Jovović, Đurđica and Vajić, Una Jovana and Miloradović, Zoran",
year = "2023",
publisher = "Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije",
journal = "Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023",
title = "Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima",
pages = "76-75",
number = "1 (supplement 1)",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1433"
}

Mihailović-Stanojević, N., Karanović, D., Grujić-Milanović, J., Ivanov, M., Jovović, Đ., Vajić, U. J.,& Miloradović, Z.. (2023). Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije., 45(1 (supplement 1)), 75-76.
https://hdl.handle.net/21.15107/rcub_rimi_1433

Mihailović-Stanojević N, Karanović D, Grujić-Milanović J, Ivanov M, Jovović Đ, Vajić UJ, Miloradović Z. Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023. 2023;45(1 (supplement 1)):75-76.
https://hdl.handle.net/21.15107/rcub_rimi_1433 .

Mihailović-Stanojević, Nevena, Karanović, Danijela, Grujić-Milanović, Jelica, Ivanov, Milan, Jovović, Đurđica, Vajić, Una Jovana, Miloradović, Zoran, "Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima" in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023, 45, no. 1 (supplement 1) (2023):75-76,
https://hdl.handle.net/21.15107/rcub_rimi_1433 .

TY  - CONF
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Životić, Maja
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1373
PB  - "Victor Babes" University of medicine and pharmacy from Timisoara
C3  - 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
T1  - Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?
EP  - 134
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1373
ER  - 

@conference{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Grujić-Milanović, Jelica and Životić, Maja and Dekanski, Dragana and Jovović, Đurđica",
year = "2023",
publisher = ""Victor Babes" University of medicine and pharmacy from Timisoara",
journal = "9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book",
title = "Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?",
pages = "134-134",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1373"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Vajić, U. J., Grujić-Milanović, J., Životić, M., Dekanski, D.,& Jovović, Đ.. (2023). Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
"Victor Babes" University of medicine and pharmacy from Timisoara., 134-134.
https://hdl.handle.net/21.15107/rcub_rimi_1373

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Vajić UJ, Grujić-Milanović J, Životić M, Dekanski D, Jovović Đ. Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book. 2023;:134-134.
https://hdl.handle.net/21.15107/rcub_rimi_1373 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Grujić-Milanović, Jelica, Životić, Maja, Dekanski, Dragana, Jovović, Đurđica, "Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?" in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book (2023):134-134,
https://hdl.handle.net/21.15107/rcub_rimi_1373 .

TY  - JOUR
AU  - Paunović, Danijela
AU  - Rajković, Jovana
AU  - Novaković, Radmila
AU  - Grujić-Milanović, Jelica
AU  - Mekky, Reham Hassan
AU  - Popa, Dragos
AU  - Calina, Daniela
AU  - Sharifi-Rad, Javad
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1387
AB  - Gossypol, a polyphenolic aldehyde derived from cottonseed plants, has seen a transformation in its pharmaceutical application from a male contraceptive to a candidate for cancer therapy. This shift is supported by its recognized antitumor properties, which have prompted its investigation in the treatment of various cancers and related inflammatory conditions. This review synthesizes the current understanding of gossypol as an anticancer agent, focusing on its pharmacological mechanisms, strategies to enhance its clinical efficacy, and the status of ongoing clinical evaluations.

The methodological approach to this review involved a systematic search across several scientific databases including the National Center for Biotechnology Information (NCBI), PubMed/MedLine, Google Scholar, Scopus, and TRIP. Studies were meticulously chosen to cover various aspects of gossypol, from its chemical structure and natural sources to its pharmacokinetics and confirmed anticancer efficacy. Specific MeSH terms and keywords related to gossypol’s antineoplastic applications guided the search strategy.

Results from selected pharmacological studies indicate that gossypol inhibits the Bcl-2 family of anti-apoptotic proteins, promoting apoptosis in tumor cells. Clinical trials, particularly phase I and II, reveal gossypol’s promise as an anticancer agent, demonstrating efficacy and manageable toxicity profiles. The review identifies the development of gossypol derivatives and novel carriers as avenues to enhance therapeutic outcomes and mitigate adverse effects.

Conclusively, gossypol represents a promising anticancer agent with considerable therapeutic potential. However, further research is needed to refine gossypol-based therapies, explore combination treatments, and verify their effectiveness across cancer types. The ongoing clinical trials continue to support its potential, suggesting a future where gossypol could play a significant role in cancer treatment protocols.
PB  - BioMed Central
T2  - Chinese Medicine
T1  - The potential roles of gossypol as anticancer agent: advances and future directions
IS  - 1
SP  - 163
VL  - 18
DO  - 10.1186/s13020-023-00869-8
ER  - 

@article{
author = "Paunović, Danijela and Rajković, Jovana and Novaković, Radmila and Grujić-Milanović, Jelica and Mekky, Reham Hassan and Popa, Dragos and Calina, Daniela and Sharifi-Rad, Javad",
year = "2023",
abstract = "Gossypol, a polyphenolic aldehyde derived from cottonseed plants, has seen a transformation in its pharmaceutical application from a male contraceptive to a candidate for cancer therapy. This shift is supported by its recognized antitumor properties, which have prompted its investigation in the treatment of various cancers and related inflammatory conditions. This review synthesizes the current understanding of gossypol as an anticancer agent, focusing on its pharmacological mechanisms, strategies to enhance its clinical efficacy, and the status of ongoing clinical evaluations.

The methodological approach to this review involved a systematic search across several scientific databases including the National Center for Biotechnology Information (NCBI), PubMed/MedLine, Google Scholar, Scopus, and TRIP. Studies were meticulously chosen to cover various aspects of gossypol, from its chemical structure and natural sources to its pharmacokinetics and confirmed anticancer efficacy. Specific MeSH terms and keywords related to gossypol’s antineoplastic applications guided the search strategy.

Results from selected pharmacological studies indicate that gossypol inhibits the Bcl-2 family of anti-apoptotic proteins, promoting apoptosis in tumor cells. Clinical trials, particularly phase I and II, reveal gossypol’s promise as an anticancer agent, demonstrating efficacy and manageable toxicity profiles. The review identifies the development of gossypol derivatives and novel carriers as avenues to enhance therapeutic outcomes and mitigate adverse effects.

Conclusively, gossypol represents a promising anticancer agent with considerable therapeutic potential. However, further research is needed to refine gossypol-based therapies, explore combination treatments, and verify their effectiveness across cancer types. The ongoing clinical trials continue to support its potential, suggesting a future where gossypol could play a significant role in cancer treatment protocols.",
publisher = "BioMed Central",
journal = "Chinese Medicine",
title = "The potential roles of gossypol as anticancer agent: advances and future directions",
number = "1",
pages = "163",
volume = "18",
doi = "10.1186/s13020-023-00869-8"
}

Paunović, D., Rajković, J., Novaković, R., Grujić-Milanović, J., Mekky, R. H., Popa, D., Calina, D.,& Sharifi-Rad, J.. (2023). The potential roles of gossypol as anticancer agent: advances and future directions. in Chinese Medicine
BioMed Central., 18(1), 163.
https://doi.org/10.1186/s13020-023-00869-8

Paunović D, Rajković J, Novaković R, Grujić-Milanović J, Mekky RH, Popa D, Calina D, Sharifi-Rad J. The potential roles of gossypol as anticancer agent: advances and future directions. in Chinese Medicine. 2023;18(1):163.
doi:10.1186/s13020-023-00869-8 .

Paunović, Danijela, Rajković, Jovana, Novaković, Radmila, Grujić-Milanović, Jelica, Mekky, Reham Hassan, Popa, Dragos, Calina, Daniela, Sharifi-Rad, Javad, "The potential roles of gossypol as anticancer agent: advances and future directions" in Chinese Medicine, 18, no. 1 (2023):163,
https://doi.org/10.1186/s13020-023-00869-8 . .

TY  - JOUR
AU  - Rajković, Jovana
AU  - Novaković, Radmila
AU  - Grujić-Milanović, Jelica
AU  - Ydyrys, Alibek
AU  - Ablaikhanova, Nurzhanat
AU  - Calina, Daniela
AU  - Sharifi-Rad, Javad
AU  - Al-Omari, Basem
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1302
AB  - Calotropin is a pharmacologically active compound isolated from milkweed plants like Calotropis procera, Calotropis gigantea, and Asclepias currasavica that belong to the Asclepiadaceae family. All of these plants are recognised as medical traditional plants used in Asian countries. Calotropin is identified as a highly potent cardenolide that has a similar chemical structure to cardiac glycosides (such as digoxin and digitoxin). During the last few years, cytotoxic and antitumor effects of cardenolides glycosides have been reported more frequently. Among cardenolides, calotropin is identified as the most promising agent. In this updated and comprehensive review, we aimed to analyze and discuss the specific mechanisms and molecular targets of calotropin in cancer treatment to open new perspectives for the adjuvant treatment of different types of cancer. The effects of calotropin on cancer have been extensively studied in preclinical pharmacological studies in vitro using cancer cell lines and in vivo in experimental animal models that have targeted antitumor mechanisms and anticancer signaling pathways. The analyzed information from the specialized literature was obtained from scientific databases until December 2022, mainly from PubMed/MedLine, Google Scholar, Scopus, Web of Science, and Science Direct databases using specific MeSH search terms. The results of our analysis demonstrate that calotropin can be a potential chemotherapeutic/chemopreventive adjunctive agent in cancer pharmacotherapeutic management.
PB  - Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - An updated pharmacological insight into calotropin as a potential therapeutic agent in cancer
SP  - 1160616
VL  - 14
DO  - 10.3389/fphar.2023.1160616
ER  - 

@article{
author = "Rajković, Jovana and Novaković, Radmila and Grujić-Milanović, Jelica and Ydyrys, Alibek and Ablaikhanova, Nurzhanat and Calina, Daniela and Sharifi-Rad, Javad and Al-Omari, Basem",
year = "2023",
abstract = "Calotropin is a pharmacologically active compound isolated from milkweed plants like Calotropis procera, Calotropis gigantea, and Asclepias currasavica that belong to the Asclepiadaceae family. All of these plants are recognised as medical traditional plants used in Asian countries. Calotropin is identified as a highly potent cardenolide that has a similar chemical structure to cardiac glycosides (such as digoxin and digitoxin). During the last few years, cytotoxic and antitumor effects of cardenolides glycosides have been reported more frequently. Among cardenolides, calotropin is identified as the most promising agent. In this updated and comprehensive review, we aimed to analyze and discuss the specific mechanisms and molecular targets of calotropin in cancer treatment to open new perspectives for the adjuvant treatment of different types of cancer. The effects of calotropin on cancer have been extensively studied in preclinical pharmacological studies in vitro using cancer cell lines and in vivo in experimental animal models that have targeted antitumor mechanisms and anticancer signaling pathways. The analyzed information from the specialized literature was obtained from scientific databases until December 2022, mainly from PubMed/MedLine, Google Scholar, Scopus, Web of Science, and Science Direct databases using specific MeSH search terms. The results of our analysis demonstrate that calotropin can be a potential chemotherapeutic/chemopreventive adjunctive agent in cancer pharmacotherapeutic management.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "An updated pharmacological insight into calotropin as a potential therapeutic agent in cancer",
pages = "1160616",
volume = "14",
doi = "10.3389/fphar.2023.1160616"
}

Rajković, J., Novaković, R., Grujić-Milanović, J., Ydyrys, A., Ablaikhanova, N., Calina, D., Sharifi-Rad, J.,& Al-Omari, B.. (2023). An updated pharmacological insight into calotropin as a potential therapeutic agent in cancer. in Frontiers in Pharmacology
Frontiers Media S.A.., 14, 1160616.
https://doi.org/10.3389/fphar.2023.1160616

Rajković J, Novaković R, Grujić-Milanović J, Ydyrys A, Ablaikhanova N, Calina D, Sharifi-Rad J, Al-Omari B. An updated pharmacological insight into calotropin as a potential therapeutic agent in cancer. in Frontiers in Pharmacology. 2023;14:1160616.
doi:10.3389/fphar.2023.1160616 .

Rajković, Jovana, Novaković, Radmila, Grujić-Milanović, Jelica, Ydyrys, Alibek, Ablaikhanova, Nurzhanat, Calina, Daniela, Sharifi-Rad, Javad, Al-Omari, Basem, "An updated pharmacological insight into calotropin as a potential therapeutic agent in cancer" in Frontiers in Pharmacology, 14 (2023):1160616,
https://doi.org/10.3389/fphar.2023.1160616 . .

TY  - CONF
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Vajić, Una Jovana
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Miloradović, Zoran
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1371
PB  - "Victor Babes" University of medicine and pharmacy from Timisoara
C3  - 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
T1  - Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1371
ER  - 

@conference{
author = "Mihailović-Stanojević, Nevena and Karanović, Danijela and Grujić-Milanović, Jelica and Vajić, Una Jovana and Ivanov, Milan and Jovović, Đurđica and Miloradović, Zoran",
year = "2023",
publisher = ""Victor Babes" University of medicine and pharmacy from Timisoara",
journal = "9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book",
title = "Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1371"
}

Mihailović-Stanojević, N., Karanović, D., Grujić-Milanović, J., Vajić, U. J., Ivanov, M., Jovović, Đ.,& Miloradović, Z.. (2023). Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
"Victor Babes" University of medicine and pharmacy from Timisoara., 82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1371

Mihailović-Stanojević N, Karanović D, Grujić-Milanović J, Vajić UJ, Ivanov M, Jovović Đ, Miloradović Z. Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book. 2023;:82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1371 .

Mihailović-Stanojević, Nevena, Karanović, Danijela, Grujić-Milanović, Jelica, Vajić, Una Jovana, Ivanov, Milan, Jovović, Đurđica, Miloradović, Zoran, "Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat" in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book (2023):82-82,
https://hdl.handle.net/21.15107/rcub_rimi_1371 .

TY  - CONF
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1372
PB  - "Victor Babes" University of medicine and pharmacy from Timisoara
C3  - 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
T1  - Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats
EP  - 131
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1372
ER  - 

@conference{
author = "Vajić, Una Jovana and Mihailović-Stanojević, Nevena and Ivanov, Milan and Karanović, Danijela and Jovović, Đurđica and Grujić-Milanović, Jelica and Miloradović, Zoran",
year = "2023",
publisher = ""Victor Babes" University of medicine and pharmacy from Timisoara",
journal = "9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book",
title = "Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1372"
}

Vajić, U. J., Mihailović-Stanojević, N., Ivanov, M., Karanović, D., Jovović, Đ., Grujić-Milanović, J.,& Miloradović, Z.. (2023). Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
"Victor Babes" University of medicine and pharmacy from Timisoara., 131-131.
https://hdl.handle.net/21.15107/rcub_rimi_1372

Vajić UJ, Mihailović-Stanojević N, Ivanov M, Karanović D, Jovović Đ, Grujić-Milanović J, Miloradović Z. Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book. 2023;:131-131.
https://hdl.handle.net/21.15107/rcub_rimi_1372 .

Vajić, Una Jovana, Mihailović-Stanojević, Nevena, Ivanov, Milan, Karanović, Danijela, Jovović, Đurđica, Grujić-Milanović, Jelica, Miloradović, Zoran, "Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats" in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book (2023):131-131,
https://hdl.handle.net/21.15107/rcub_rimi_1372 .

TY  - CONF
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Mihailović-Stanojević, Nevena
AU  - Grujić-Milanović, Jelica
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Marković-Lipkovski, Jasmina
AU  - Jovović, Đurđica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1376
AB  - Hipertenzija, koja se izmedu ostalog karakteriše i deficitom vasodilatatornog azotnog monoksida
(AM), mačajno pogoršava kliničku sliku postishernične akutne bubrežne slabosti (ABS) kod ljudi. Ponovno vraćanje krvi u bubrežno tkivo, samo po sebi dovodi do masovne produkcije slobodnih vrsta kiseonika (SVK) i pogoršanja ishemijske povrede. Ispitivali smo potencijalni zaštitni efekat primene donora AM-a, kao i uklanjanja SVK na sistemske i bubrežne hemodinamske parametre, kao i na strukturu i funkciju bubrega pacova sa urodenom hipertenzijom (PUH), kojima je izazivana postishemična ABS. Za navedene potrebe, PUH su dobijali donor AM, L-arginin, prirodni sakupljač SRK vitamin C (Vit C) ili sintetski mimetik enzima superoksid dismutase TEMPOL, tokom ishemije-reperfuzije bubrega u trajanju od 40 minuta. Tretman L-argininom je poboljšao sistemsku i bubreżnu hemodinarniku, ali nije uspeo da smanji ośtećenje bubrega. Nasuprot tome, intenzitet nekroze i dilatacije tubula su bili mačajno smanjeni kod pacova u ABS+Vit C grupi u poredenju sa kontrolnom ABS grupom. Uprkos smanjenju lipidne peroksidacije u plazmi, kao i renalnog vaskularnog otpora, tretman TEMPOL-om nije uspeo da ublaži reperfuzijom nastala oštećenja bubreżnog tkiva kod PUH. Naši rezultati ukazuju na to da SVK učestvuju u reperfuzionim oštećenjima bubrega, a da su sintetski mimetik superoksid dismutaze i donor AM neefikasni u zaštiti glomerula i tubula nakon nastanka ABS kod PUH. Sa druge strane, naši rezultati sugerišu daje Vit C, prirodni antioksidant, hemoprotektant umerenog zaśtitnog dejstva protiv ishemijsko-reperfuzionih povreda bubrega u hipertenzivnim okolnostima. Ova zaštita je većinski povezana sa direktnim uklanjanjem SVK.
AB  - Hypertension, which is among others characterized by reducing of nitric oxide (NO) mediated vasodilatation, strongly aggravates postischemic acute kidney injury (AKI) in humans. Kidney reperfusion itself increases ischemic injury through the massive production of oxygen-free radicals. We investigated protective potential of NO supplementation and superoxide radical scavenging on systemic and renal haemodynamic parameters as well as kidney function and structure in spontaneously hypertensive rats (SHR) with induced postischemic AKI. For these purposes, SHR were subjected to NO donor L-arginine, natural oxidant scavenger vitamin C (Vit C) or synthetic superoxide dismutase mimetic TEMPOL during 40 minutes kidney ischaemia-reperfusion period. L-arginine supplementation improves systemic and renal hemodynamic but failed to reduce kidney tubular injuries. In opposite, intensity of tubular necrosis and dilatation was markedly reduced in AKI+Vit C group in comparison to AKI control. Despite blunting of lipid peroxidation in plasma and diminishing of renal vascular resistance, TEMPOL treatment also failed to reduce kidney tissue injuries induced by reperfusion in SHR. Our results suggest that superoxide radicals participate in kidney reperfusion injuries while synthetic superoxide dismutase and NO supplementation are ineffective in glomerular and tubular protection after AKI episode in hypertensive rats. On the other hand, our results promote natural antioxidant, vitamin C, as a moderately effective chemoprotectant against kidney ischemia-reperfusion injuries in hypertensive surrounding. Protection is mainly related to the direct superoxide radical scavenging.
PB  - Niš : Punta
C3  - Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
T1  - Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension
T1  - Bioraspoloživost azotnog monoksida i antioksidativna odbrana kao ciljevi terapije u udruženom modelu akutne bubrežne slabosti i hipertenzije
EP  - 22
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1376
ER  - 

@conference{
author = "Miloradović, Zoran and Ivanov, Milan and Mihailović-Stanojević, Nevena and Grujić-Milanović, Jelica and Karanović, Danijela and Vajić, Una Jovana and Marković-Lipkovski, Jasmina and Jovović, Đurđica",
year = "2023",
abstract = "Hipertenzija, koja se izmedu ostalog karakteriše i deficitom vasodilatatornog azotnog monoksida
(AM), mačajno pogoršava kliničku sliku postishernične akutne bubrežne slabosti (ABS) kod ljudi. Ponovno vraćanje krvi u bubrežno tkivo, samo po sebi dovodi do masovne produkcije slobodnih vrsta kiseonika (SVK) i pogoršanja ishemijske povrede. Ispitivali smo potencijalni zaštitni efekat primene donora AM-a, kao i uklanjanja SVK na sistemske i bubrežne hemodinamske parametre, kao i na strukturu i funkciju bubrega pacova sa urodenom hipertenzijom (PUH), kojima je izazivana postishemična ABS. Za navedene potrebe, PUH su dobijali donor AM, L-arginin, prirodni sakupljač SRK vitamin C (Vit C) ili sintetski mimetik enzima superoksid dismutase TEMPOL, tokom ishemije-reperfuzije bubrega u trajanju od 40 minuta. Tretman L-argininom je poboljšao sistemsku i bubreżnu hemodinarniku, ali nije uspeo da smanji ośtećenje bubrega. Nasuprot tome, intenzitet nekroze i dilatacije tubula su bili mačajno smanjeni kod pacova u ABS+Vit C grupi u poredenju sa kontrolnom ABS grupom. Uprkos smanjenju lipidne peroksidacije u plazmi, kao i renalnog vaskularnog otpora, tretman TEMPOL-om nije uspeo da ublaži reperfuzijom nastala oštećenja bubreżnog tkiva kod PUH. Naši rezultati ukazuju na to da SVK učestvuju u reperfuzionim oštećenjima bubrega, a da su sintetski mimetik superoksid dismutaze i donor AM neefikasni u zaštiti glomerula i tubula nakon nastanka ABS kod PUH. Sa druge strane, naši rezultati sugerišu daje Vit C, prirodni antioksidant, hemoprotektant umerenog zaśtitnog dejstva protiv ishemijsko-reperfuzionih povreda bubrega u hipertenzivnim okolnostima. Ova zaštita je većinski povezana sa direktnim uklanjanjem SVK., Hypertension, which is among others characterized by reducing of nitric oxide (NO) mediated vasodilatation, strongly aggravates postischemic acute kidney injury (AKI) in humans. Kidney reperfusion itself increases ischemic injury through the massive production of oxygen-free radicals. We investigated protective potential of NO supplementation and superoxide radical scavenging on systemic and renal haemodynamic parameters as well as kidney function and structure in spontaneously hypertensive rats (SHR) with induced postischemic AKI. For these purposes, SHR were subjected to NO donor L-arginine, natural oxidant scavenger vitamin C (Vit C) or synthetic superoxide dismutase mimetic TEMPOL during 40 minutes kidney ischaemia-reperfusion period. L-arginine supplementation improves systemic and renal hemodynamic but failed to reduce kidney tubular injuries. In opposite, intensity of tubular necrosis and dilatation was markedly reduced in AKI+Vit C group in comparison to AKI control. Despite blunting of lipid peroxidation in plasma and diminishing of renal vascular resistance, TEMPOL treatment also failed to reduce kidney tissue injuries induced by reperfusion in SHR. Our results suggest that superoxide radicals participate in kidney reperfusion injuries while synthetic superoxide dismutase and NO supplementation are ineffective in glomerular and tubular protection after AKI episode in hypertensive rats. On the other hand, our results promote natural antioxidant, vitamin C, as a moderately effective chemoprotectant against kidney ischemia-reperfusion injuries in hypertensive surrounding. Protection is mainly related to the direct superoxide radical scavenging.",
publisher = "Niš : Punta",
journal = "Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia",
title = "Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension, Bioraspoloživost azotnog monoksida i antioksidativna odbrana kao ciljevi terapije u udruženom modelu akutne bubrežne slabosti i hipertenzije",
pages = "22-16",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1376"
}

Miloradović, Z., Ivanov, M., Mihailović-Stanojević, N., Grujić-Milanović, J., Karanović, D., Vajić, U. J., Marković-Lipkovski, J.,& Jovović, Đ.. (2023). Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
Niš : Punta., 16-22.
https://hdl.handle.net/21.15107/rcub_rimi_1376

Miloradović Z, Ivanov M, Mihailović-Stanojević N, Grujić-Milanović J, Karanović D, Vajić UJ, Marković-Lipkovski J, Jovović Đ. Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia. 2023;:16-22.
https://hdl.handle.net/21.15107/rcub_rimi_1376 .

Miloradović, Zoran, Ivanov, Milan, Mihailović-Stanojević, Nevena, Grujić-Milanović, Jelica, Karanović, Danijela, Vajić, Una Jovana, Marković-Lipkovski, Jasmina, Jovović, Đurđica, "Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension" in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia (2023):16-22,
https://hdl.handle.net/21.15107/rcub_rimi_1376 .

TY  - JOUR
AU  - Mandić, Danijela
AU  - Nežić, Lana
AU  - Amdžić, Ljiljana
AU  - Vojinović, Nataša
AU  - Gajanin, Radoslav
AU  - Popović, Miroslav
AU  - Đeri, Jugoslav
AU  - Todorović Balint, Milena
AU  - Dumanović, Jelena
AU  - Milovanović, Zoran
AU  - Grujić-Milanović, Jelica
AU  - Škrbić, Ranko
AU  - Jaćević, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1343
AB  - Background: Approximately 40% of patients with diffuse large B-cell lymphoma (DLBCL) experience treatment resistance to the first-line R-CHOP regimen. ATP binding cassette (ABC) transporters and survivin might play a role in multidrug resistance (MDR) in various tumors. The aim was to investigate if the coexpression of ABC transporters and survivin was associated with R-CHOP treatment response. Methods: The expression of Bcl-2, survivin, P-glycoprotein/ABCB1, MRP1/ABCC1, and BCRP/ABCC2 was analyzed using immunohistochemistry in tumor specimens obtained from patients with DLBCL, and classified according to the treatment response as Remission, Relapsed, and (primary) Refractory groups. All patients received R-CHOP or equivalent treatment. Results: Bcl-2 was in strong positive correlation with clinical parameters and all biomarkers except P-gp/ABCB1. The overexpression of MRP1/ABCC1, survivin, and BCRP/ABCC2 presented as high immunoreactive scores (IRSs) was detected in the Refractory and Relapsed groups (p < 0.05 vs. Remission), respectively, whereas the IRS of P-gp/ABCB1 was low. Significant correlations were found among either MRP1/ABCC1 and survivin or BCRP/ABCC2 in the Refractory and Relapsed groups, respectively. In multiple linear regression analysis, ECOG status along with MRP1/ABCC1 or survivin and BRCP/ABCG2 was significantly associated with the prediction of the R-CHOP treatment response. Conclusions: DLBCL might harbor certain molecular signatures such as MRP1/ABCC1, survivin, and BCRP/ABCC2 overexpression that can predict resistance to R-CHOP.
PB  - MDPI (Multidisciplinary Digital Publishing Institute)
T2  - Cancers
T2  - Cancers
T1  - Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment
IS  - 16
SP  - 4106
VL  - 15
DO  - 10.3390/cancers15164106
ER  - 

@article{
author = "Mandić, Danijela and Nežić, Lana and Amdžić, Ljiljana and Vojinović, Nataša and Gajanin, Radoslav and Popović, Miroslav and Đeri, Jugoslav and Todorović Balint, Milena and Dumanović, Jelena and Milovanović, Zoran and Grujić-Milanović, Jelica and Škrbić, Ranko and Jaćević, Vesna",
year = "2023",
abstract = "Background: Approximately 40% of patients with diffuse large B-cell lymphoma (DLBCL) experience treatment resistance to the first-line R-CHOP regimen. ATP binding cassette (ABC) transporters and survivin might play a role in multidrug resistance (MDR) in various tumors. The aim was to investigate if the coexpression of ABC transporters and survivin was associated with R-CHOP treatment response. Methods: The expression of Bcl-2, survivin, P-glycoprotein/ABCB1, MRP1/ABCC1, and BCRP/ABCC2 was analyzed using immunohistochemistry in tumor specimens obtained from patients with DLBCL, and classified according to the treatment response as Remission, Relapsed, and (primary) Refractory groups. All patients received R-CHOP or equivalent treatment. Results: Bcl-2 was in strong positive correlation with clinical parameters and all biomarkers except P-gp/ABCB1. The overexpression of MRP1/ABCC1, survivin, and BCRP/ABCC2 presented as high immunoreactive scores (IRSs) was detected in the Refractory and Relapsed groups (p < 0.05 vs. Remission), respectively, whereas the IRS of P-gp/ABCB1 was low. Significant correlations were found among either MRP1/ABCC1 and survivin or BCRP/ABCC2 in the Refractory and Relapsed groups, respectively. In multiple linear regression analysis, ECOG status along with MRP1/ABCC1 or survivin and BRCP/ABCG2 was significantly associated with the prediction of the R-CHOP treatment response. Conclusions: DLBCL might harbor certain molecular signatures such as MRP1/ABCC1, survivin, and BCRP/ABCC2 overexpression that can predict resistance to R-CHOP.",
publisher = "MDPI (Multidisciplinary Digital Publishing Institute)",
journal = "Cancers, Cancers",
title = "Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment",
number = "16",
pages = "4106",
volume = "15",
doi = "10.3390/cancers15164106"
}

Mandić, D., Nežić, L., Amdžić, L., Vojinović, N., Gajanin, R., Popović, M., Đeri, J., Todorović Balint, M., Dumanović, J., Milovanović, Z., Grujić-Milanović, J., Škrbić, R.,& Jaćević, V.. (2023). Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment. in Cancers
MDPI (Multidisciplinary Digital Publishing Institute)., 15(16), 4106.
https://doi.org/10.3390/cancers15164106

Mandić D, Nežić L, Amdžić L, Vojinović N, Gajanin R, Popović M, Đeri J, Todorović Balint M, Dumanović J, Milovanović Z, Grujić-Milanović J, Škrbić R, Jaćević V. Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment. in Cancers. 2023;15(16):4106.
doi:10.3390/cancers15164106 .

Mandić, Danijela, Nežić, Lana, Amdžić, Ljiljana, Vojinović, Nataša, Gajanin, Radoslav, Popović, Miroslav, Đeri, Jugoslav, Todorović Balint, Milena, Dumanović, Jelena, Milovanović, Zoran, Grujić-Milanović, Jelica, Škrbić, Ranko, Jaćević, Vesna, "Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment" in Cancers, 15, no. 16 (2023):4106,
https://doi.org/10.3390/cancers15164106 . .

TY  - JOUR
AU  - Jaćević, Vesna
AU  - Dumanović, Jelena
AU  - Grujić-Milanović, Jelica
AU  - Milovanović, Zoran
AU  - Amidžić, Ljiljana
AU  - Vojinović, Nataša
AU  - Nežić, Lana
AU  - Marković, Bojan
AU  - Dobričić, Vladimir
AU  - Milosavljević, Petar
AU  - Nepovimova, Eugenie
AU  - Kuča, Kamil
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1342
AB  - Oxidative stress status and morphological injuries in the brain of Wistar rats induced by repeated application of selected acetylcholinesterase reactivators - asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Each oxime in a dose of 0.1 of LD50/kg im was given 2x/week for 4 weeks. Markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, glutathione reductase, GR, and glutathione peroxidase, GPx), were estimated in the brain tissue homogenates on day 35 of the study. Brain alterations were carefully quantified by semiquantitative grading scales - brain damage score (BDS). Oxidative stress parameters, MDA and AOPP were significantly highest in the asoxime-, obidoxime- and K075-treated groups (p < 0.001). The activity of SOD and CAT was significantly elevated in the obidoxime-, K048-, and K075-treated groups (p < 0.001). Besides, GR was markedly decreased in the obidoxime- and K074-treated groups (p < 0.01), while treatment with K048, K074 and K075 induced extremely high elevation in GPx levels (p < 0.001). In the same groups of rats, brain alterations associated with polymorphonuclear cell infiltrate were significantly more severe than those observed in animals receiving only asoxime or K027 (p < 0.001). The presented results confirmed that treatment with different oximes significantly improved the oxidative status and attenuated signs of inflammation in rats' brains. Presented results, together with our previously published data can help to predict likely adverse systemic toxic effects, and target organ systems, which are crucial for establishing risk categories, as well as in dose selection of K-oximes as drug candidates.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T2  - Chemico-Biological InteractionsChem Biol Interact
T1  - Oxidative stress status assessment of rats' brains injury following subacute exposure to K-oximes
SP  - 110658
VL  - 383
DO  - 10.1016/j.cbi.2023.110658
ER  - 

@article{
author = "Jaćević, Vesna and Dumanović, Jelena and Grujić-Milanović, Jelica and Milovanović, Zoran and Amidžić, Ljiljana and Vojinović, Nataša and Nežić, Lana and Marković, Bojan and Dobričić, Vladimir and Milosavljević, Petar and Nepovimova, Eugenie and Kuča, Kamil",
year = "2023",
abstract = "Oxidative stress status and morphological injuries in the brain of Wistar rats induced by repeated application of selected acetylcholinesterase reactivators - asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Each oxime in a dose of 0.1 of LD50/kg im was given 2x/week for 4 weeks. Markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, glutathione reductase, GR, and glutathione peroxidase, GPx), were estimated in the brain tissue homogenates on day 35 of the study. Brain alterations were carefully quantified by semiquantitative grading scales - brain damage score (BDS). Oxidative stress parameters, MDA and AOPP were significantly highest in the asoxime-, obidoxime- and K075-treated groups (p < 0.001). The activity of SOD and CAT was significantly elevated in the obidoxime-, K048-, and K075-treated groups (p < 0.001). Besides, GR was markedly decreased in the obidoxime- and K074-treated groups (p < 0.01), while treatment with K048, K074 and K075 induced extremely high elevation in GPx levels (p < 0.001). In the same groups of rats, brain alterations associated with polymorphonuclear cell infiltrate were significantly more severe than those observed in animals receiving only asoxime or K027 (p < 0.001). The presented results confirmed that treatment with different oximes significantly improved the oxidative status and attenuated signs of inflammation in rats' brains. Presented results, together with our previously published data can help to predict likely adverse systemic toxic effects, and target organ systems, which are crucial for establishing risk categories, as well as in dose selection of K-oximes as drug candidates.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions, Chemico-Biological InteractionsChem Biol Interact",
title = "Oxidative stress status assessment of rats' brains injury following subacute exposure to K-oximes",
pages = "110658",
volume = "383",
doi = "10.1016/j.cbi.2023.110658"
}

Jaćević, V., Dumanović, J., Grujić-Milanović, J., Milovanović, Z., Amidžić, L., Vojinović, N., Nežić, L., Marković, B., Dobričić, V., Milosavljević, P., Nepovimova, E.,& Kuča, K.. (2023). Oxidative stress status assessment of rats' brains injury following subacute exposure to K-oximes. in Chemico-Biological Interactions
Elsevier., 383, 110658.
https://doi.org/10.1016/j.cbi.2023.110658

Jaćević V, Dumanović J, Grujić-Milanović J, Milovanović Z, Amidžić L, Vojinović N, Nežić L, Marković B, Dobričić V, Milosavljević P, Nepovimova E, Kuča K. Oxidative stress status assessment of rats' brains injury following subacute exposure to K-oximes. in Chemico-Biological Interactions. 2023;383:110658.
doi:10.1016/j.cbi.2023.110658 .

Jaćević, Vesna, Dumanović, Jelena, Grujić-Milanović, Jelica, Milovanović, Zoran, Amidžić, Ljiljana, Vojinović, Nataša, Nežić, Lana, Marković, Bojan, Dobričić, Vladimir, Milosavljević, Petar, Nepovimova, Eugenie, Kuča, Kamil, "Oxidative stress status assessment of rats' brains injury following subacute exposure to K-oximes" in Chemico-Biological Interactions, 383 (2023):110658,
https://doi.org/10.1016/j.cbi.2023.110658 . .

TY  - JOUR
AU  - Grujić-Milanović, Jelica
AU  - Jaćević, Vesna
AU  - Miloradović, Zoran
AU  - Milanović, Slađan
AU  - Jovović, Đurđica
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1263
AB  - Objective Background: The main cause of death among patients with malignant hypertension is a kidney failure. The promising field in essential and malignant hypertension therapy could be centered on the amelioration of oxidative stress using antioxidant molecules like resveratrol. Resveratrol is a potent antioxidative agent naturally occurred in many plants that possess health-promoting properties. Methods: In the present study, we investigated the therapeutic potential of resveratrol, a polyphenol with anti-oxidative activity, in NG-L-Arginine Methyl Ester (L-NAME) treated spontaneously hypertensive rats (SHR) - malignantly hypertensive rats (MHR). Results: Resveratrol significantly improves oxidative damages by modulation of antioxidant enzymes and suppression of prooxidant factors in the kidney tissue of MHR. Enhanced antioxidant defense in the kidney improves renal function and ameliorates the morphological changes in this target organ. Besides, protective properties of resveratrol are followed by the restoration of the nitrogen oxide (NO) pathway. 4) Conclusion: Antioxidant therapy with resveratrol could represent promising therapeutical approach in hypertension, especially malignant, against kidney damage.
T2  - Biomedicine & Pharmacotherapy
T1  - Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability
SP  - 113642
VL  - 154
DO  - 10.1016/j.biopha.2022.113642
ER  - 

@article{
author = "Grujić-Milanović, Jelica and Jaćević, Vesna and Miloradović, Zoran and Milanović, Slađan and Jovović, Đurđica and Ivanov, Milan and Karanović, Danijela and Vajić, Una Jovana and Mihailović-Stanojević, Nevena",
year = "2022",
abstract = "Objective Background: The main cause of death among patients with malignant hypertension is a kidney failure. The promising field in essential and malignant hypertension therapy could be centered on the amelioration of oxidative stress using antioxidant molecules like resveratrol. Resveratrol is a potent antioxidative agent naturally occurred in many plants that possess health-promoting properties. Methods: In the present study, we investigated the therapeutic potential of resveratrol, a polyphenol with anti-oxidative activity, in NG-L-Arginine Methyl Ester (L-NAME) treated spontaneously hypertensive rats (SHR) - malignantly hypertensive rats (MHR). Results: Resveratrol significantly improves oxidative damages by modulation of antioxidant enzymes and suppression of prooxidant factors in the kidney tissue of MHR. Enhanced antioxidant defense in the kidney improves renal function and ameliorates the morphological changes in this target organ. Besides, protective properties of resveratrol are followed by the restoration of the nitrogen oxide (NO) pathway. 4) Conclusion: Antioxidant therapy with resveratrol could represent promising therapeutical approach in hypertension, especially malignant, against kidney damage.",
journal = "Biomedicine & Pharmacotherapy",
title = "Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability",
pages = "113642",
volume = "154",
doi = "10.1016/j.biopha.2022.113642"
}

Grujić-Milanović, J., Jaćević, V., Miloradović, Z., Milanović, S., Jovović, Đ., Ivanov, M., Karanović, D., Vajić, U. J.,& Mihailović-Stanojević, N.. (2022). Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability. in Biomedicine & Pharmacotherapy, 154, 113642.
https://doi.org/10.1016/j.biopha.2022.113642

Grujić-Milanović J, Jaćević V, Miloradović Z, Milanović S, Jovović Đ, Ivanov M, Karanović D, Vajić UJ, Mihailović-Stanojević N. Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability. in Biomedicine & Pharmacotherapy. 2022;154:113642.
doi:10.1016/j.biopha.2022.113642 .

Grujić-Milanović, Jelica, Jaćević, Vesna, Miloradović, Zoran, Milanović, Slađan, Jovović, Đurđica, Ivanov, Milan, Karanović, Danijela, Vajić, Una Jovana, Mihailović-Stanojević, Nevena, "Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability" in Biomedicine & Pharmacotherapy, 154 (2022):113642,
https://doi.org/10.1016/j.biopha.2022.113642 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Grujić-Milanović, Jelica
AU  - Vejnović, Branislav
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1237
AB  - Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.
PB  - Taylor & Francis
T2  - Italian Journal of Animal Science
T1  - Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows
EP  - 858
IS  - 1
SP  - 847
VL  - 21
DO  - 10.1080/1828051X.2022.2070034
ER  - 

@article{
author = "Drvenica, Ivana and Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Grujić-Milanović, Jelica and Vejnović, Branislav and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna",
year = "2022",
abstract = "Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.",
publisher = "Taylor & Francis",
journal = "Italian Journal of Animal Science",
title = "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows",
pages = "858-847",
number = "1",
volume = "21",
doi = "10.1080/1828051X.2022.2070034"
}

Drvenica, I., Stojić, M., Fratrić, N., Kovačić, M., Grujić-Milanović, J., Vejnović, B., Marković, D., Gvozdić, D.,& Ilić, V.. (2022). Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science
Taylor & Francis., 21(1), 847-858.
https://doi.org/10.1080/1828051X.2022.2070034

Drvenica I, Stojić M, Fratrić N, Kovačić M, Grujić-Milanović J, Vejnović B, Marković D, Gvozdić D, Ilić V. Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science. 2022;21(1):847-858.
doi:10.1080/1828051X.2022.2070034 .

Drvenica, Ivana, Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Grujić-Milanović, Jelica, Vejnović, Branislav, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows" in Italian Journal of Animal Science, 21, no. 1 (2022):847-858,
https://doi.org/10.1080/1828051X.2022.2070034 . .

TY  - CONF
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Vajić, Una Jovana
AU  - Jovović, Đurđica
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1438
AB  - Uvod. Reaktivne vrste kiseonika (RVK), važni signalni molekuli, u uslovima prekomerne produkcije dovode do poremećaja funkcije i strukture organa i tkiva, uključujući bubreg. RVK doprinose dijabetičnim nefropatijama, hipertenzijom i ishemijom izazvanim bolestima bubrega, kao i toksičnim nefropatijama. Cilj i metode. Proučavali smo, na eksperimentalnim modelima, uticaj RVK na nastanak i razvoj fokalno-segmentne glomerulo-skleroze i postishemične akutne bubrežne slabosti. Rezultati. Naši rezultati su pokazali štetno delovanje reperfuzijom indukovane produkcije RVK na protok krvi kroz bubrežnu arteriju, glomerusku i tubulsku funkciju bubrega, kao i strukturu bubrežnog tkiva. Sa druge strane, uočili smo brojne interakcije ovih molekula sa azotnim monoksidom, sa kojim formiraju visoko reaktivne peroksinitrite i oštećuju tkivo bubrega. Evidentna je i povezanost RVK sa renin angiotenzin sistemom, jer je blokada receptora za angiotenzin 2 dovela do poboljšanja strukture i funcije bubrega u oba modela nefropatija. Akutno uklanjanje RVK mimeticima superoksid dismutaze, kao i pretretman hiperbaričnim kiseonikom, su takođe dovodili do usporavanja toka i razvoja nefropatija, sa određenim, neočekivanim, odstupanjima u ishodima kombinovane terapije. Zaključak. RVK igraju značajnu ulogu u patofiziološkim mehanizmima nefropatija, a strategije njihovog neutralisanja predstavljaju obećavajući pristup terapiji ovih poremećaja.
PB  - Beograd: Srpsko biološko društvo
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.
T1  - Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja
SP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1438
ER  - 

@conference{
author = "Miloradović, Zoran and Mihailović-Stanojević, Nevena and Ivanov, Milan and Karanović, Danijela and Grujić-Milanović, Jelica and Vajić, Una Jovana and Jovović, Đurđica",
year = "2022",
abstract = "Uvod. Reaktivne vrste kiseonika (RVK), važni signalni molekuli, u uslovima prekomerne produkcije dovode do poremećaja funkcije i strukture organa i tkiva, uključujući bubreg. RVK doprinose dijabetičnim nefropatijama, hipertenzijom i ishemijom izazvanim bolestima bubrega, kao i toksičnim nefropatijama. Cilj i metode. Proučavali smo, na eksperimentalnim modelima, uticaj RVK na nastanak i razvoj fokalno-segmentne glomerulo-skleroze i postishemične akutne bubrežne slabosti. Rezultati. Naši rezultati su pokazali štetno delovanje reperfuzijom indukovane produkcije RVK na protok krvi kroz bubrežnu arteriju, glomerusku i tubulsku funkciju bubrega, kao i strukturu bubrežnog tkiva. Sa druge strane, uočili smo brojne interakcije ovih molekula sa azotnim monoksidom, sa kojim formiraju visoko reaktivne peroksinitrite i oštećuju tkivo bubrega. Evidentna je i povezanost RVK sa renin angiotenzin sistemom, jer je blokada receptora za angiotenzin 2 dovela do poboljšanja strukture i funcije bubrega u oba modela nefropatija. Akutno uklanjanje RVK mimeticima superoksid dismutaze, kao i pretretman hiperbaričnim kiseonikom, su takođe dovodili do usporavanja toka i razvoja nefropatija, sa određenim, neočekivanim, odstupanjima u ishodima kombinovane terapije. Zaključak. RVK igraju značajnu ulogu u patofiziološkim mehanizmima nefropatija, a strategije njihovog neutralisanja predstavljaju obećavajući pristup terapiji ovih poremećaja.",
publisher = "Beograd: Srpsko biološko društvo",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.",
title = "Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja",
pages = "6",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1438"
}

Miloradović, Z., Mihailović-Stanojević, N., Ivanov, M., Karanović, D., Grujić-Milanović, J., Vajić, U. J.,& Jovović, Đ.. (2022). Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.
Beograd: Srpsko biološko društvo., 6.
https://hdl.handle.net/21.15107/rcub_rimi_1438

Miloradović Z, Mihailović-Stanojević N, Ivanov M, Karanović D, Grujić-Milanović J, Vajić UJ, Jovović Đ. Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.. 2022;:6.
https://hdl.handle.net/21.15107/rcub_rimi_1438 .

Miloradović, Zoran, Mihailović-Stanojević, Nevena, Ivanov, Milan, Karanović, Danijela, Grujić-Milanović, Jelica, Vajić, Una Jovana, Jovović, Đurđica, "Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija. (2022):6,
https://hdl.handle.net/21.15107/rcub_rimi_1438 .

TY  - JOUR
AU  - Grujić-Milanović, Jelica
AU  - Jaćević, Vesna
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Milosavljević, Ivica
AU  - Milanović, Slađan
AU  - Mihailović-Stanojević, Nevena
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1137
AB  - Hypertension is one of the most prevalent and powerful contributors of cardiovascular diseases. Malignant hypertension is a relatively rare but extremely severe form of hypertension accompanied with heart, brain, and renal impairment. Resveratrol, a recently described grape-derived, polyphenolic antioxidant molecule, has been proposed as an effective agent in the prevention of cardiovascular diseases. This study was designed to examine chronic resveratrol administration on blood pressure, oxidative stress, and inflammation, with special emphasis on cardiac structure and function in two models of experimental hypertension. The experiments were performed in spontaneously (SHRs) and malignantly hypertensive rats (MHRs). The chronic administration of resveratrol significantly decreased blood pressure in both spontaneously and malignant hypertensive animals. The resveratrol treatment ameliorated morphological changes in the heart tissue. The immunohistochemistry of the heart tissue after resveratrol treatment showed that both TGF-β and Bax were not present in the myocytes of SHRs and were present mainly in the myocytes of MHRs. Resveratrol suppressed lipid peroxidation and significantly improved oxidative status and release of NO. These results suggest that resveratrol prevents hypertrophic and apoptotic consequences induced by high blood pressure with more pronounced effects in malignant hypertension.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties
IS  - 9
SP  - 5006
VL  - 22
DO  - 10.3390/ijms22095006
ER  - 

@article{
author = "Grujić-Milanović, Jelica and Jaćević, Vesna and Miloradović, Zoran and Jovović, Đurđica and Milosavljević, Ivica and Milanović, Slađan and Mihailović-Stanojević, Nevena",
year = "2021",
abstract = "Hypertension is one of the most prevalent and powerful contributors of cardiovascular diseases. Malignant hypertension is a relatively rare but extremely severe form of hypertension accompanied with heart, brain, and renal impairment. Resveratrol, a recently described grape-derived, polyphenolic antioxidant molecule, has been proposed as an effective agent in the prevention of cardiovascular diseases. This study was designed to examine chronic resveratrol administration on blood pressure, oxidative stress, and inflammation, with special emphasis on cardiac structure and function in two models of experimental hypertension. The experiments were performed in spontaneously (SHRs) and malignantly hypertensive rats (MHRs). The chronic administration of resveratrol significantly decreased blood pressure in both spontaneously and malignant hypertensive animals. The resveratrol treatment ameliorated morphological changes in the heart tissue. The immunohistochemistry of the heart tissue after resveratrol treatment showed that both TGF-β and Bax were not present in the myocytes of SHRs and were present mainly in the myocytes of MHRs. Resveratrol suppressed lipid peroxidation and significantly improved oxidative status and release of NO. These results suggest that resveratrol prevents hypertrophic and apoptotic consequences induced by high blood pressure with more pronounced effects in malignant hypertension.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties",
number = "9",
pages = "5006",
volume = "22",
doi = "10.3390/ijms22095006"
}

Grujić-Milanović, J., Jaćević, V., Miloradović, Z., Jovović, Đ., Milosavljević, I., Milanović, S.,& Mihailović-Stanojević, N.. (2021). Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties. in International Journal of Molecular Sciences
MDPI., 22(9), 5006.
https://doi.org/10.3390/ijms22095006

Grujić-Milanović J, Jaćević V, Miloradović Z, Jovović Đ, Milosavljević I, Milanović S, Mihailović-Stanojević N. Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties. in International Journal of Molecular Sciences. 2021;22(9):5006.
doi:10.3390/ijms22095006 .

Grujić-Milanović, Jelica, Jaćević, Vesna, Miloradović, Zoran, Jovović, Đurđica, Milosavljević, Ivica, Milanović, Slađan, Mihailović-Stanojević, Nevena, "Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties" in International Journal of Molecular Sciences, 22, no. 9 (2021):5006,
https://doi.org/10.3390/ijms22095006 . .

TY  - JOUR
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Marković-Lipkovski, Jasmina
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/5
AB  - Olive (Olea europaea L.) leaf extract (OLE) possesses powerful antioxidant, antihyperlipidemic, and anti-inflammatory properties. The aim was to investigated the effects of OLE on the hyperlipidemia, antioxidant defense, heme oxygenase/biliverdin reductase (HO/BVR) pathway, inflammation, and fibrosis in spontaneously hypertensive rats with focal segmental glomerulosclerosis (FSGS, a progressive form of chronic kidney disease) induced by adriamycin (2 mg/kg, i.v., twice in a 21-day period). Daily treatment of OLE (80 mg/kg, p.o.) for 6 weeks suppressed protein oxidation and lipid peroxidation (p  lt  .01 and p  lt  .001, respectively), significantly increased antioxidant enzymes activities and normalized antioxidant capacity, leading to the improvement of antioxidant defense independently of the HO/BVR pathway. Furthermore, the values of triglycerides (p  lt  .01), total, and low-density lipoprotein cholesterol (p  lt  .05, both) were improved by OLE. OLE strongly prevented glomerulosclerosis, interstitial inflammation, and fibrosis (renal injury score, FSGS: 8 +/- 0.45 vs. FSGS+OLE: 4.20 +/- 1.07; p  lt  .01), as evidenced by normalized fibronectin content (p  lt  .001), suppressed interstitial inflammatory cells infiltration and collagen deposition, without changing cytokines expressions. OLE decreased blood pressure with a tendency to reduce urine albumin loss. These data suggest that OLE may be effective in slowing down the progression of FSGS.
PB  - Wiley, Hoboken
T2  - Phytotherapy Research
T1  - Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis
EP  - 1545
IS  - 3
SP  - 1534
VL  - 35
DO  - 10.1002/ptr.6920
ER  - 

@article{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Grujić-Milanović, Jelica and Marković-Lipkovski, Jasmina and Dekanski, Dragana and Jovović, Đurđica",
year = "2021",
abstract = "Olive (Olea europaea L.) leaf extract (OLE) possesses powerful antioxidant, antihyperlipidemic, and anti-inflammatory properties. The aim was to investigated the effects of OLE on the hyperlipidemia, antioxidant defense, heme oxygenase/biliverdin reductase (HO/BVR) pathway, inflammation, and fibrosis in spontaneously hypertensive rats with focal segmental glomerulosclerosis (FSGS, a progressive form of chronic kidney disease) induced by adriamycin (2 mg/kg, i.v., twice in a 21-day period). Daily treatment of OLE (80 mg/kg, p.o.) for 6 weeks suppressed protein oxidation and lipid peroxidation (p  lt  .01 and p  lt  .001, respectively), significantly increased antioxidant enzymes activities and normalized antioxidant capacity, leading to the improvement of antioxidant defense independently of the HO/BVR pathway. Furthermore, the values of triglycerides (p  lt  .01), total, and low-density lipoprotein cholesterol (p  lt  .05, both) were improved by OLE. OLE strongly prevented glomerulosclerosis, interstitial inflammation, and fibrosis (renal injury score, FSGS: 8 +/- 0.45 vs. FSGS+OLE: 4.20 +/- 1.07; p  lt  .01), as evidenced by normalized fibronectin content (p  lt  .001), suppressed interstitial inflammatory cells infiltration and collagen deposition, without changing cytokines expressions. OLE decreased blood pressure with a tendency to reduce urine albumin loss. These data suggest that OLE may be effective in slowing down the progression of FSGS.",
publisher = "Wiley, Hoboken",
journal = "Phytotherapy Research",
title = "Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis",
pages = "1545-1534",
number = "3",
volume = "35",
doi = "10.1002/ptr.6920"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Vajić, U. J., Grujić-Milanović, J., Marković-Lipkovski, J., Dekanski, D.,& Jovović, Đ.. (2021). Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis. in Phytotherapy Research
Wiley, Hoboken., 35(3), 1534-1545.
https://doi.org/10.1002/ptr.6920

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Vajić UJ, Grujić-Milanović J, Marković-Lipkovski J, Dekanski D, Jovović Đ. Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis. in Phytotherapy Research. 2021;35(3):1534-1545.
doi:10.1002/ptr.6920 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Grujić-Milanović, Jelica, Marković-Lipkovski, Jasmina, Dekanski, Dragana, Jovović, Đurđica, "Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis" in Phytotherapy Research, 35, no. 3 (2021):1534-1545,
https://doi.org/10.1002/ptr.6920 . .

TY  - JOUR
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Banjac, Vojislav V.
AU  - Vidosavljević, Strahinja
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Jovović, Đurđica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1132
AB  - Lifestyle modifications such as increase in high-fat food consumption importantly increases the risks for cardiovascular disease. The principal objective of this study is to analyze effects of different high fat diet (HFD) sources on haemodynamic parameters, lipid and oxidative profile, myeloperoxidase activity, and markers of inflammation (IL-6/pentraxin-3). HFD containing 20% of fat, provided by lard (saturated) or soybean oil (unsaturated), as well as control diet were administering to three groups (L, SO and C). Food efficiency ratio and plasma lipids were significantly elevated in both HFD groups. However, only SO group showed an increase in systolic arterial pressure, oxidative stress index, myeloperoxidase activity, liver lipids as well as markers of inflammation: IL-6 and pentraxin-3 (PTX3). In summary, these results indicate inflammogenic potential of excessive soybean oil consumption in triggering liver damage.
PB  - Elsevier
T2  - Biomedicine & Pharmacotherapy
T1  - Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats
SP  - 111691
VL  - 139
DO  - 10.1016/j.biopha.2021.111691
ER  - 

@article{
author = "Grujić-Milanović, Jelica and Miloradović, Zoran and Mihailović-Stanojević, Nevena and Banjac, Vojislav V. and Vidosavljević, Strahinja and Ivanov, Milan and Karanović, Danijela and Vajić, Una Jovana and Jovović, Đurđica",
year = "2021",
abstract = "Lifestyle modifications such as increase in high-fat food consumption importantly increases the risks for cardiovascular disease. The principal objective of this study is to analyze effects of different high fat diet (HFD) sources on haemodynamic parameters, lipid and oxidative profile, myeloperoxidase activity, and markers of inflammation (IL-6/pentraxin-3). HFD containing 20% of fat, provided by lard (saturated) or soybean oil (unsaturated), as well as control diet were administering to three groups (L, SO and C). Food efficiency ratio and plasma lipids were significantly elevated in both HFD groups. However, only SO group showed an increase in systolic arterial pressure, oxidative stress index, myeloperoxidase activity, liver lipids as well as markers of inflammation: IL-6 and pentraxin-3 (PTX3). In summary, these results indicate inflammogenic potential of excessive soybean oil consumption in triggering liver damage.",
publisher = "Elsevier",
journal = "Biomedicine & Pharmacotherapy",
title = "Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats",
pages = "111691",
volume = "139",
doi = "10.1016/j.biopha.2021.111691"
}

Grujić-Milanović, J., Miloradović, Z., Mihailović-Stanojević, N., Banjac, V. V., Vidosavljević, S., Ivanov, M., Karanović, D., Vajić, U. J.,& Jovović, Đ.. (2021). Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats. in Biomedicine & Pharmacotherapy
Elsevier., 139, 111691.
https://doi.org/10.1016/j.biopha.2021.111691

Grujić-Milanović J, Miloradović Z, Mihailović-Stanojević N, Banjac VV, Vidosavljević S, Ivanov M, Karanović D, Vajić UJ, Jovović Đ. Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats. in Biomedicine & Pharmacotherapy. 2021;139:111691.
doi:10.1016/j.biopha.2021.111691 .

Grujić-Milanović, Jelica, Miloradović, Zoran, Mihailović-Stanojević, Nevena, Banjac, Vojislav V., Vidosavljević, Strahinja, Ivanov, Milan, Karanović, Danijela, Vajić, Una Jovana, Jovović, Đurđica, "Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats" in Biomedicine & Pharmacotherapy, 139 (2021):111691,
https://doi.org/10.1016/j.biopha.2021.111691 . .

TY  - JOUR
AU  - Bruić, Marija
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Živković, Lada
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1164
AB  - Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.
PB  - Elsevier
T2  - The International Journal of Biochemistry & Cell Biology
T1  - DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats
SP  - 106088
VL  - 141
DO  - 10.1016/j.biocel.2021.106088
ER  - 

@article{
author = "Bruić, Marija and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Živković, Lada and Mihailović-Stanojević, Nevena and Karanović, Danijela and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.",
publisher = "Elsevier",
journal = "The International Journal of Biochemistry & Cell Biology",
title = "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats",
pages = "106088",
volume = "141",
doi = "10.1016/j.biocel.2021.106088"
}

Bruić, M., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Živković, L., Mihailović-Stanojević, N., Karanović, D.,& Spremo-Potparević, B.. (2021). DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in The International Journal of Biochemistry & Cell Biology
Elsevier., 141, 106088.
https://doi.org/10.1016/j.biocel.2021.106088

Bruić M, Grujić-Milanović J, Miloradović Z, Jovović Đ, Živković L, Mihailović-Stanojević N, Karanović D, Spremo-Potparević B. DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in The International Journal of Biochemistry & Cell Biology. 2021;141:106088.
doi:10.1016/j.biocel.2021.106088 .

Bruić, Marija, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Živković, Lada, Mihailović-Stanojević, Nevena, Karanović, Danijela, Spremo-Potparević, Biljana, "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats" in The International Journal of Biochemistry & Cell Biology, 141 (2021):106088,
https://doi.org/10.1016/j.biocel.2021.106088 . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Fratrić, Natalija
AU  - Arsić, Aleksandra
AU  - Mojsilović, Slavko
AU  - Drvenica, Ivana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Ilić, Vesna
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1010
AB  - Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes
EP  - 74
SP  - 63
VL  - 133
DO  - 10.1016/j.rvsc.2020.09.004
ER  - 

@article{
author = "Kovačić, Marijana and Fratrić, Natalija and Arsić, Aleksandra and Mojsilović, Slavko and Drvenica, Ivana and Marković, Dragana and Maslovarić, Irina and Grujić-Milanović, Jelica and Ivanov, Milan and Ilić, Vesna",
year = "2020",
abstract = "Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes",
pages = "74-63",
volume = "133",
doi = "10.1016/j.rvsc.2020.09.004"
}

Kovačić, M., Fratrić, N., Arsić, A., Mojsilović, S., Drvenica, I., Marković, D., Maslovarić, I., Grujić-Milanović, J., Ivanov, M.,& Ilić, V.. (2020). Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 133, 63-74.
https://doi.org/10.1016/j.rvsc.2020.09.004

Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić-Milanović J, Ivanov M, Ilić V. Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science. 2020;133:63-74.
doi:10.1016/j.rvsc.2020.09.004 .

Kovačić, Marijana, Fratrić, Natalija, Arsić, Aleksandra, Mojsilović, Slavko, Drvenica, Ivana, Marković, Dragana, Maslovarić, Irina, Grujić-Milanović, Jelica, Ivanov, Milan, Ilić, Vesna, "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes" in Research in Veterinary Science, 133 (2020):63-74,
https://doi.org/10.1016/j.rvsc.2020.09.004 . .

TY  - CONF
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Grujić-Milanović, Jelica
AU  - Vajić, Una Jovana
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1048
PB  - Oxford Univ Press, Oxford
C3  - Nephrology Dialysis Transplantation
T1  - Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis
EP  - 1242
SP  - 1242
VL  - 35
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1048
ER  - 

@conference{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Grujić-Milanović, Jelica and Vajić, Una Jovana and Dekanski, Dragana and Jovović, Đurđica",
year = "2020",
publisher = "Oxford Univ Press, Oxford",
journal = "Nephrology Dialysis Transplantation",
title = "Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis",
pages = "1242-1242",
volume = "35",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1048"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Grujić-Milanović, J., Vajić, U. J., Dekanski, D.,& Jovović, Đ.. (2020). Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis. in Nephrology Dialysis Transplantation
Oxford Univ Press, Oxford., 35, 1242-1242.
https://hdl.handle.net/21.15107/rcub_rimi_1048

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Grujić-Milanović J, Vajić UJ, Dekanski D, Jovović Đ. Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis. in Nephrology Dialysis Transplantation. 2020;35:1242-1242.
https://hdl.handle.net/21.15107/rcub_rimi_1048 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Grujić-Milanović, Jelica, Vajić, Una Jovana, Dekanski, Dragana, Jovović, Đurđica, "Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis" in Nephrology Dialysis Transplantation, 35 (2020):1242-1242,
https://hdl.handle.net/21.15107/rcub_rimi_1048 .

TY  - CONF
AU  - Ivanov, Milan
AU  - Brkić, Predrag
AU  - Mihailović-Stanojević, Nevena
AU  - Jovović, Đurđica
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Jeremić, Rada
AU  - Đelić, Marina
AU  - Milacić, D.
AU  - Miloradović, Zoran
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/852
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Hypertension
T1  - Hyperbaric oxygen preconditioning improves renal haemodynamic and kidneyfunction in spontaneously hypertensive rats with ischemic acute kidney injury
EP  - E77
SP  - E77
VL  - 36
DO  - 10.1097/01.hjh.0000539184.07956.a9
ER  - 

@conference{
author = "Ivanov, Milan and Brkić, Predrag and Mihailović-Stanojević, Nevena and Jovović, Đurđica and Vajić, Una Jovana and Karanović, Danijela and Grujić-Milanović, Jelica and Jeremić, Rada and Đelić, Marina and Milacić, D. and Miloradović, Zoran",
year = "2018",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Hypertension",
title = "Hyperbaric oxygen preconditioning improves renal haemodynamic and kidneyfunction in spontaneously hypertensive rats with ischemic acute kidney injury",
pages = "E77-E77",
volume = "36",
doi = "10.1097/01.hjh.0000539184.07956.a9"
}

Ivanov, M., Brkić, P., Mihailović-Stanojević, N., Jovović, Đ., Vajić, U. J., Karanović, D., Grujić-Milanović, J., Jeremić, R., Đelić, M., Milacić, D.,& Miloradović, Z.. (2018). Hyperbaric oxygen preconditioning improves renal haemodynamic and kidneyfunction in spontaneously hypertensive rats with ischemic acute kidney injury. in Journal of Hypertension
Lippincott Williams & Wilkins, Philadelphia., 36, E77-E77.
https://doi.org/10.1097/01.hjh.0000539184.07956.a9

Ivanov M, Brkić P, Mihailović-Stanojević N, Jovović Đ, Vajić UJ, Karanović D, Grujić-Milanović J, Jeremić R, Đelić M, Milacić D, Miloradović Z. Hyperbaric oxygen preconditioning improves renal haemodynamic and kidneyfunction in spontaneously hypertensive rats with ischemic acute kidney injury. in Journal of Hypertension. 2018;36:E77-E77.
doi:10.1097/01.hjh.0000539184.07956.a9 .

Ivanov, Milan, Brkić, Predrag, Mihailović-Stanojević, Nevena, Jovović, Đurđica, Vajić, Una Jovana, Karanović, Danijela, Grujić-Milanović, Jelica, Jeremić, Rada, Đelić, Marina, Milacić, D., Miloradović, Zoran, "Hyperbaric oxygen preconditioning improves renal haemodynamic and kidneyfunction in spontaneously hypertensive rats with ischemic acute kidney injury" in Journal of Hypertension, 36 (2018):E77-E77,
https://doi.org/10.1097/01.hjh.0000539184.07956.a9 . .

TY  - JOUR
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Savikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/846
AB  - Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Phytomedicine
T1  - Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats
EP  - 45
SP  - 39
VL  - 46
DO  - 10.1016/j.phymed.2018.04.037
ER  - 

@article{
author = "Vajić, Una Jovana and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Ivanov, Milan and Karanović, Danijela and Savikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2018",
abstract = "Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Phytomedicine",
title = "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats",
pages = "45-39",
volume = "46",
doi = "10.1016/j.phymed.2018.04.037"
}

Vajić, U. J., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Ivanov, M., Karanović, D., Savikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2018). Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 46, 39-45.
https://doi.org/10.1016/j.phymed.2018.04.037

Vajić UJ, Grujić-Milanović J, Miloradović Z, Jovović Đ, Ivanov M, Karanović D, Savikin K, Bugarski B, Mihailović-Stanojević N. Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine. 2018;46:39-45.
doi:10.1016/j.phymed.2018.04.037 .

Vajić, Una Jovana, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Ivanov, Milan, Karanović, Danijela, Savikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats" in Phytomedicine, 46 (2018):39-45,
https://doi.org/10.1016/j.phymed.2018.04.037 . .

TY  - JOUR
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Grujić-Milanović, Jelica
AU  - Karanović, Danijela
AU  - Dekanski, Dragana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/876
AB  - Haemodynamic alterations in carotid and renal arteries are associated with the severity of target organ damage in patients with hypertension. Dietary habits, such as the Mediterranean diet, regulate blood pressure and oxidative stress, thus reduce the mortality rate due to cardiovascular diseases. In this study, our aim was to evaluate the reducing activity, antioxidant capacity and metal chelating ability of standardized Olea europaea L. leaf extract (OLE), and to test its (5, 25, 50 mg/kg) acute in vivo effects, as well as oleuropein's (OP, 10 mg/kg) on oxidative stress, carotid, renal and systemic haemodynamic parameters (blood pressure, heart rate, cardiac output, peripheral resistance) in spontaneously hypertensive rats (SHR). OLE has a higher antioxidative capacity than BHT, higher reducing ability than vitamin C, and 23 times lower capacity for metal ion chelation than EDTA. All three doses of OLE, and OP, improved oxidative stress in SHR. OLE5 improved carotid and renal haemodynamics, without significant effects on systemic haemodynamics. Two different mechanisms of antihypertensive responses to OLE were observed, OLE25 was most effective in reducing cardiovascular risks by improving systemic and regional (carotid and renal) haemodynamics, peripheral and regional vascular resistance. OLE50 causes the improvement of blood pressure and cardiac performances, but tends to retain elevated vascular resistance, therefore, reducing the inflow of blood into the brain and kidneys of the SHR. The OP did not alter systemic or regional haemodynamics, suggesting others constituents responsible for changes of cardiac function, as well as carotid and renal haemodynamics in response to OLE50.
PB  - Excli Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein
EP  - 44
SP  - 29
VL  - 17
DO  - 10.17179/excli2017-1002
ER  - 

@article{
author = "Ivanov, Milan and Vajić, Una Jovana and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Jovović, Đurđica and Grujić-Milanović, Jelica and Karanović, Danijela and Dekanski, Dragana",
year = "2018",
abstract = "Haemodynamic alterations in carotid and renal arteries are associated with the severity of target organ damage in patients with hypertension. Dietary habits, such as the Mediterranean diet, regulate blood pressure and oxidative stress, thus reduce the mortality rate due to cardiovascular diseases. In this study, our aim was to evaluate the reducing activity, antioxidant capacity and metal chelating ability of standardized Olea europaea L. leaf extract (OLE), and to test its (5, 25, 50 mg/kg) acute in vivo effects, as well as oleuropein's (OP, 10 mg/kg) on oxidative stress, carotid, renal and systemic haemodynamic parameters (blood pressure, heart rate, cardiac output, peripheral resistance) in spontaneously hypertensive rats (SHR). OLE has a higher antioxidative capacity than BHT, higher reducing ability than vitamin C, and 23 times lower capacity for metal ion chelation than EDTA. All three doses of OLE, and OP, improved oxidative stress in SHR. OLE5 improved carotid and renal haemodynamics, without significant effects on systemic haemodynamics. Two different mechanisms of antihypertensive responses to OLE were observed, OLE25 was most effective in reducing cardiovascular risks by improving systemic and regional (carotid and renal) haemodynamics, peripheral and regional vascular resistance. OLE50 causes the improvement of blood pressure and cardiac performances, but tends to retain elevated vascular resistance, therefore, reducing the inflow of blood into the brain and kidneys of the SHR. The OP did not alter systemic or regional haemodynamics, suggesting others constituents responsible for changes of cardiac function, as well as carotid and renal haemodynamics in response to OLE50.",
publisher = "Excli Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein",
pages = "44-29",
volume = "17",
doi = "10.17179/excli2017-1002"
}

Ivanov, M., Vajić, U. J., Mihailović-Stanojević, N., Miloradović, Z., Jovović, Đ., Grujić-Milanović, J., Karanović, D.,& Dekanski, D.. (2018). Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein. in EXCLI Journal
Excli Journal Managing Office, Dortmund., 17, 29-44.
https://doi.org/10.17179/excli2017-1002

Ivanov M, Vajić UJ, Mihailović-Stanojević N, Miloradović Z, Jovović Đ, Grujić-Milanović J, Karanović D, Dekanski D. Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein. in EXCLI Journal. 2018;17:29-44.
doi:10.17179/excli2017-1002 .

Ivanov, Milan, Vajić, Una Jovana, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Jovović, Đurđica, Grujić-Milanović, Jelica, Karanović, Danijela, Dekanski, Dragana, "Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein" in EXCLI Journal, 17 (2018):29-44,
https://doi.org/10.17179/excli2017-1002 . .

TY  - JOUR
AU  - Ćujić, Nada
AU  - Savikin, Katarina
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Jovović, Đurđica
AU  - Mihailović-Stanojević, Nevena
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/894
AB  - The potential beneficial effects of chronic consumption of dried chokeberry fruit extract (DCE) were evaluated in the present in vivo study. The study concentrates on haemodynamic parameters, lipid profile and oxidative stress in spontaneously hypertensive rats. DCE was characterized by HPLC/DAD method and individual anthocyanins, phenolic acids, and flavonoids were determined. Four week administration of DCE, rich in polyphenols, especially anthocyanins, significantly reduced systolic (P  lt  0.05), pulse pressure (P  lt  0.05) compared to control group, probably via significantly increased diuresis (P  lt  0.05). Significant decrease of plasma and erythrocytes TBARS was also found in the treated group. DCE consumption reduced lipid peroxidation through improving plasma FRAP reducing (P  lt  0.01). The activity of SOD in the treated group had been significantly lower (P  lt  0.01) compared to the control group. These results suggest that DCE could exert beneficial effects on cardiovascular health and has a potential as a nutritional supplement in arterial hypertension.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats
EP  - 339
SP  - 330
VL  - 44
DO  - 10.1016/j.jff.2018.02.027
ER  - 

@article{
author = "Ćujić, Nada and Savikin, Katarina and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Karanović, Danijela and Grujić-Milanović, Jelica and Jovović, Đurđica and Mihailović-Stanojević, Nevena",
year = "2018",
abstract = "The potential beneficial effects of chronic consumption of dried chokeberry fruit extract (DCE) were evaluated in the present in vivo study. The study concentrates on haemodynamic parameters, lipid profile and oxidative stress in spontaneously hypertensive rats. DCE was characterized by HPLC/DAD method and individual anthocyanins, phenolic acids, and flavonoids were determined. Four week administration of DCE, rich in polyphenols, especially anthocyanins, significantly reduced systolic (P  lt  0.05), pulse pressure (P  lt  0.05) compared to control group, probably via significantly increased diuresis (P  lt  0.05). Significant decrease of plasma and erythrocytes TBARS was also found in the treated group. DCE consumption reduced lipid peroxidation through improving plasma FRAP reducing (P  lt  0.01). The activity of SOD in the treated group had been significantly lower (P  lt  0.01) compared to the control group. These results suggest that DCE could exert beneficial effects on cardiovascular health and has a potential as a nutritional supplement in arterial hypertension.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats",
pages = "339-330",
volume = "44",
doi = "10.1016/j.jff.2018.02.027"
}

Ćujić, N., Savikin, K., Miloradović, Z., Ivanov, M., Vajić, U. J., Karanović, D., Grujić-Milanović, J., Jovović, Đ.,& Mihailović-Stanojević, N.. (2018). Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 44, 330-339.
https://doi.org/10.1016/j.jff.2018.02.027

Ćujić N, Savikin K, Miloradović Z, Ivanov M, Vajić UJ, Karanović D, Grujić-Milanović J, Jovović Đ, Mihailović-Stanojević N. Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats. in Journal of Functional Foods. 2018;44:330-339.
doi:10.1016/j.jff.2018.02.027 .

Ćujić, Nada, Savikin, Katarina, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Karanović, Danijela, Grujić-Milanović, Jelica, Jovović, Đurđica, Mihailović-Stanojević, Nevena, "Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats" in Journal of Functional Foods, 44 (2018):330-339,
https://doi.org/10.1016/j.jff.2018.02.027 . .