TY  - JOUR
AU  - Mandić, Danijela
AU  - Nežić, Lana
AU  - Amdžić, Ljiljana
AU  - Vojinović, Nataša
AU  - Gajanin, Radoslav
AU  - Popović, Miroslav
AU  - Đeri, Jugoslav
AU  - Todorović Balint, Milena
AU  - Dumanović, Jelena
AU  - Milovanović, Zoran
AU  - Grujić-Milanović, Jelica
AU  - Škrbić, Ranko
AU  - Jaćević, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1343
AB  - Background: Approximately 40% of patients with diffuse large B-cell lymphoma (DLBCL) experience treatment resistance to the first-line R-CHOP regimen. ATP binding cassette (ABC) transporters and survivin might play a role in multidrug resistance (MDR) in various tumors. The aim was to investigate if the coexpression of ABC transporters and survivin was associated with R-CHOP treatment response. Methods: The expression of Bcl-2, survivin, P-glycoprotein/ABCB1, MRP1/ABCC1, and BCRP/ABCC2 was analyzed using immunohistochemistry in tumor specimens obtained from patients with DLBCL, and classified according to the treatment response as Remission, Relapsed, and (primary) Refractory groups. All patients received R-CHOP or equivalent treatment. Results: Bcl-2 was in strong positive correlation with clinical parameters and all biomarkers except P-gp/ABCB1. The overexpression of MRP1/ABCC1, survivin, and BCRP/ABCC2 presented as high immunoreactive scores (IRSs) was detected in the Refractory and Relapsed groups (p < 0.05 vs. Remission), respectively, whereas the IRS of P-gp/ABCB1 was low. Significant correlations were found among either MRP1/ABCC1 and survivin or BCRP/ABCC2 in the Refractory and Relapsed groups, respectively. In multiple linear regression analysis, ECOG status along with MRP1/ABCC1 or survivin and BRCP/ABCG2 was significantly associated with the prediction of the R-CHOP treatment response. Conclusions: DLBCL might harbor certain molecular signatures such as MRP1/ABCC1, survivin, and BCRP/ABCC2 overexpression that can predict resistance to R-CHOP.
PB  - MDPI (Multidisciplinary Digital Publishing Institute)
T2  - Cancers
T2  - Cancers
T1  - Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment
IS  - 16
SP  - 4106
VL  - 15
DO  - 10.3390/cancers15164106
ER  - 

@article{
author = "Mandić, Danijela and Nežić, Lana and Amdžić, Ljiljana and Vojinović, Nataša and Gajanin, Radoslav and Popović, Miroslav and Đeri, Jugoslav and Todorović Balint, Milena and Dumanović, Jelena and Milovanović, Zoran and Grujić-Milanović, Jelica and Škrbić, Ranko and Jaćević, Vesna",
year = "2023",
abstract = "Background: Approximately 40% of patients with diffuse large B-cell lymphoma (DLBCL) experience treatment resistance to the first-line R-CHOP regimen. ATP binding cassette (ABC) transporters and survivin might play a role in multidrug resistance (MDR) in various tumors. The aim was to investigate if the coexpression of ABC transporters and survivin was associated with R-CHOP treatment response. Methods: The expression of Bcl-2, survivin, P-glycoprotein/ABCB1, MRP1/ABCC1, and BCRP/ABCC2 was analyzed using immunohistochemistry in tumor specimens obtained from patients with DLBCL, and classified according to the treatment response as Remission, Relapsed, and (primary) Refractory groups. All patients received R-CHOP or equivalent treatment. Results: Bcl-2 was in strong positive correlation with clinical parameters and all biomarkers except P-gp/ABCB1. The overexpression of MRP1/ABCC1, survivin, and BCRP/ABCC2 presented as high immunoreactive scores (IRSs) was detected in the Refractory and Relapsed groups (p < 0.05 vs. Remission), respectively, whereas the IRS of P-gp/ABCB1 was low. Significant correlations were found among either MRP1/ABCC1 and survivin or BCRP/ABCC2 in the Refractory and Relapsed groups, respectively. In multiple linear regression analysis, ECOG status along with MRP1/ABCC1 or survivin and BRCP/ABCG2 was significantly associated with the prediction of the R-CHOP treatment response. Conclusions: DLBCL might harbor certain molecular signatures such as MRP1/ABCC1, survivin, and BCRP/ABCC2 overexpression that can predict resistance to R-CHOP.",
publisher = "MDPI (Multidisciplinary Digital Publishing Institute)",
journal = "Cancers, Cancers",
title = "Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment",
number = "16",
pages = "4106",
volume = "15",
doi = "10.3390/cancers15164106"
}

Mandić, D., Nežić, L., Amdžić, L., Vojinović, N., Gajanin, R., Popović, M., Đeri, J., Todorović Balint, M., Dumanović, J., Milovanović, Z., Grujić-Milanović, J., Škrbić, R.,& Jaćević, V.. (2023). Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment. in Cancers
MDPI (Multidisciplinary Digital Publishing Institute)., 15(16), 4106.
https://doi.org/10.3390/cancers15164106

Mandić D, Nežić L, Amdžić L, Vojinović N, Gajanin R, Popović M, Đeri J, Todorović Balint M, Dumanović J, Milovanović Z, Grujić-Milanović J, Škrbić R, Jaćević V. Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment. in Cancers. 2023;15(16):4106.
doi:10.3390/cancers15164106 .

Mandić, Danijela, Nežić, Lana, Amdžić, Ljiljana, Vojinović, Nataša, Gajanin, Radoslav, Popović, Miroslav, Đeri, Jugoslav, Todorović Balint, Milena, Dumanović, Jelena, Milovanović, Zoran, Grujić-Milanović, Jelica, Škrbić, Ranko, Jaćević, Vesna, "Overexpression of MRP1/ABCC1, Survivin and BCRP/ABCC2 Predicts the Resistance of Diffuse Large B-Cell Lymphoma to R-CHOP Treatment" in Cancers, 15, no. 16 (2023):4106,
https://doi.org/10.3390/cancers15164106 . .

TY  - JOUR
AU  - Grabež, Milkica
AU  - Škrbić, Ranko
AU  - Stojiljković, Miloš P.
AU  - Vučić, Vesna M.
AU  - Grujić, Vesna Rudić
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan M.
AU  - Suručić, Relja
AU  - Šavikin, Katarina
AU  - Bigović, Dubravka
AU  - Vasiljević, Nađa
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1217
AB  - Type 2 diabetes mellitus (T2DM) is commonly associated with hyperglycemia, dyslipidemia, oxidative stress and inflammation which are well known cardiovascular risk factors. Pomegranate peel polyphenols have a proven hypolipemic, antioxidant and anti-inflammatory activity. However, there is a lack of clinical studies that would confirm its antioxidant and anti-inflammatory effects in diabetic patients. The potential of pomegranate peel extract (PoPEx) to counteract inflammation and oxidative stress in T2DM patients was investigated. For this purpose, a randomized, double-blind placebo-controlled study involving adult T2DM patients treated with PoPEx or placebo for eight-weeks was conducted. Methods: Patients were randomly divided into two groups: the first group (n = 30) received capsules containing PoPEx 250 mg twice daily, while the placebo group (n = 30) received placebo capsules twice daily. Plasma concentration of inflammatory factors (interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and high sensitivity C reactive protein (hsCRP)), oxidative stress biomarkers (thiobarbituric acid reactive substances (TBARS), nitrites (NO2−), superoxide anion radical (O2−), hydrogen peroxide (H2O2), total antioxidant capacity (TAC)), homocysteine and lipid profile were analyzed. Results: The PoPEx treatment showed a significant reduction of inflammatory factors (IL-6, TNF-α, hsCRP), oxidative stress biomarkers (TBARS, NO2−, O2−) and homocysteine, while the TAC was increased. Moreover, a significant improvement in lipid profile was observed in the PoPEx group. Additional analysis showed a significant inverse correlation between the decrements of all measured inflammatory markers and TAC in the PoPEx group. Conclusions: The study demonstrated that eight-week-long PoPEx administration had favorable effects on inflammatory status and oxidative stress biomarkers in diabetic patients.
T2  - Reviews in Cardiovascular Medicine
T2  - Reviews in Cardiovascular Medicine
T1  - A prospective, randomized, double-blind, placebo-controlled trial of polyphenols on the outcomes of inflammatory factors and oxidative stress in patients with type 2 diabetes mellitus
IS  - 2
SP  - 57
VL  - 23
DO  - 10.31083/j.rcm2302057
ER  - 

@article{
author = "Grabež, Milkica and Škrbić, Ranko and Stojiljković, Miloš P. and Vučić, Vesna M. and Grujić, Vesna Rudić and Jakovljević, Vladimir and Đurić, Dragan M. and Suručić, Relja and Šavikin, Katarina and Bigović, Dubravka and Vasiljević, Nađa",
year = "2022",
abstract = "Type 2 diabetes mellitus (T2DM) is commonly associated with hyperglycemia, dyslipidemia, oxidative stress and inflammation which are well known cardiovascular risk factors. Pomegranate peel polyphenols have a proven hypolipemic, antioxidant and anti-inflammatory activity. However, there is a lack of clinical studies that would confirm its antioxidant and anti-inflammatory effects in diabetic patients. The potential of pomegranate peel extract (PoPEx) to counteract inflammation and oxidative stress in T2DM patients was investigated. For this purpose, a randomized, double-blind placebo-controlled study involving adult T2DM patients treated with PoPEx or placebo for eight-weeks was conducted. Methods: Patients were randomly divided into two groups: the first group (n = 30) received capsules containing PoPEx 250 mg twice daily, while the placebo group (n = 30) received placebo capsules twice daily. Plasma concentration of inflammatory factors (interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and high sensitivity C reactive protein (hsCRP)), oxidative stress biomarkers (thiobarbituric acid reactive substances (TBARS), nitrites (NO2−), superoxide anion radical (O2−), hydrogen peroxide (H2O2), total antioxidant capacity (TAC)), homocysteine and lipid profile were analyzed. Results: The PoPEx treatment showed a significant reduction of inflammatory factors (IL-6, TNF-α, hsCRP), oxidative stress biomarkers (TBARS, NO2−, O2−) and homocysteine, while the TAC was increased. Moreover, a significant improvement in lipid profile was observed in the PoPEx group. Additional analysis showed a significant inverse correlation between the decrements of all measured inflammatory markers and TAC in the PoPEx group. Conclusions: The study demonstrated that eight-week-long PoPEx administration had favorable effects on inflammatory status and oxidative stress biomarkers in diabetic patients.",
journal = "Reviews in Cardiovascular Medicine, Reviews in Cardiovascular Medicine",
title = "A prospective, randomized, double-blind, placebo-controlled trial of polyphenols on the outcomes of inflammatory factors and oxidative stress in patients with type 2 diabetes mellitus",
number = "2",
pages = "57",
volume = "23",
doi = "10.31083/j.rcm2302057"
}

Grabež, M., Škrbić, R., Stojiljković, M. P., Vučić, V. M., Grujić, V. R., Jakovljević, V., Đurić, D. M., Suručić, R., Šavikin, K., Bigović, D.,& Vasiljević, N.. (2022). A prospective, randomized, double-blind, placebo-controlled trial of polyphenols on the outcomes of inflammatory factors and oxidative stress in patients with type 2 diabetes mellitus. in Reviews in Cardiovascular Medicine, 23(2), 57.
https://doi.org/10.31083/j.rcm2302057

Grabež M, Škrbić R, Stojiljković MP, Vučić VM, Grujić VR, Jakovljević V, Đurić DM, Suručić R, Šavikin K, Bigović D, Vasiljević N. A prospective, randomized, double-blind, placebo-controlled trial of polyphenols on the outcomes of inflammatory factors and oxidative stress in patients with type 2 diabetes mellitus. in Reviews in Cardiovascular Medicine. 2022;23(2):57.
doi:10.31083/j.rcm2302057 .

Grabež, Milkica, Škrbić, Ranko, Stojiljković, Miloš P., Vučić, Vesna M., Grujić, Vesna Rudić, Jakovljević, Vladimir, Đurić, Dragan M., Suručić, Relja, Šavikin, Katarina, Bigović, Dubravka, Vasiljević, Nađa, "A prospective, randomized, double-blind, placebo-controlled trial of polyphenols on the outcomes of inflammatory factors and oxidative stress in patients with type 2 diabetes mellitus" in Reviews in Cardiovascular Medicine, 23, no. 2 (2022):57,
https://doi.org/10.31083/j.rcm2302057 . .

TY  - JOUR
AU  - Grabež, Milkica
AU  - Škrbić, Ranko
AU  - Stojiljković, Miloš P.
AU  - Rudić-Grujić, Vesna
AU  - Paunović, Marija P.
AU  - Arsić, Aleksandra
AU  - Petrović, Snježana
AU  - Vučić, Vesna M.
AU  - Mirjanić-Azarić, Bosa
AU  - Savikin, Katarina
AU  - Menković, Nebojša
AU  - Janković, Teodora
AU  - Vasiljević, Nada
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1033
AB  - Pomegranate peel contains high levels of various phytochemicals. We evaluated the effects of pomegranate peel extract (PoPEx) consumption on plasma lipid profile, fatty acids (FA) level and blood pressure (BP) in patients with diabetes mellitus type 2 (DMT2). Thirty-seven subjects were recruited in this double blind, placebo controlled randomized trial. The study group (n = 19) received over 8 week's capsules containing PoPEx twice a daily, while the placebo group received placebo. Treatment with PoPEx induced a significant lowering of both systolic and diastolic BP. The plasma levels of triglycerides, low-density lipoprotein cholesterol/high-density lipoprotein cholesterol ratio (LDL-C/HDL-C), and HbA1c were significantly decreased, while the level of HDL-C was significantly increased, compared with placebo intake. Moreover, the PoPEX treatment significantly improved the plasma lipids fatty acids content. It is concluded that consumption of PoPEx in DMT2 subject had favourable effects on some metabolic parameters, BP, lipid profile and plasma lipid FA composition.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Beneficial effects of pomegranate peel extract on plasma lipid profile, fatty acids levels and blood pressure in patients with diabetes mellitus type-2: A randomized, double-blind, placebo-controlled study
SP  - 103692
VL  - 64
DO  - 10.1016/j.jff.2019.103692
ER  - 

@article{
author = "Grabež, Milkica and Škrbić, Ranko and Stojiljković, Miloš P. and Rudić-Grujić, Vesna and Paunović, Marija P. and Arsić, Aleksandra and Petrović, Snježana and Vučić, Vesna M. and Mirjanić-Azarić, Bosa and Savikin, Katarina and Menković, Nebojša and Janković, Teodora and Vasiljević, Nada",
year = "2020",
abstract = "Pomegranate peel contains high levels of various phytochemicals. We evaluated the effects of pomegranate peel extract (PoPEx) consumption on plasma lipid profile, fatty acids (FA) level and blood pressure (BP) in patients with diabetes mellitus type 2 (DMT2). Thirty-seven subjects were recruited in this double blind, placebo controlled randomized trial. The study group (n = 19) received over 8 week's capsules containing PoPEx twice a daily, while the placebo group received placebo. Treatment with PoPEx induced a significant lowering of both systolic and diastolic BP. The plasma levels of triglycerides, low-density lipoprotein cholesterol/high-density lipoprotein cholesterol ratio (LDL-C/HDL-C), and HbA1c were significantly decreased, while the level of HDL-C was significantly increased, compared with placebo intake. Moreover, the PoPEX treatment significantly improved the plasma lipids fatty acids content. It is concluded that consumption of PoPEx in DMT2 subject had favourable effects on some metabolic parameters, BP, lipid profile and plasma lipid FA composition.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Beneficial effects of pomegranate peel extract on plasma lipid profile, fatty acids levels and blood pressure in patients with diabetes mellitus type-2: A randomized, double-blind, placebo-controlled study",
pages = "103692",
volume = "64",
doi = "10.1016/j.jff.2019.103692"
}

Grabež, M., Škrbić, R., Stojiljković, M. P., Rudić-Grujić, V., Paunović, M. P., Arsić, A., Petrović, S., Vučić, V. M., Mirjanić-Azarić, B., Savikin, K., Menković, N., Janković, T.,& Vasiljević, N.. (2020). Beneficial effects of pomegranate peel extract on plasma lipid profile, fatty acids levels and blood pressure in patients with diabetes mellitus type-2: A randomized, double-blind, placebo-controlled study. in Journal of Functional Foods
Elsevier, Amsterdam., 64, 103692.
https://doi.org/10.1016/j.jff.2019.103692

Grabež M, Škrbić R, Stojiljković MP, Rudić-Grujić V, Paunović MP, Arsić A, Petrović S, Vučić VM, Mirjanić-Azarić B, Savikin K, Menković N, Janković T, Vasiljević N. Beneficial effects of pomegranate peel extract on plasma lipid profile, fatty acids levels and blood pressure in patients with diabetes mellitus type-2: A randomized, double-blind, placebo-controlled study. in Journal of Functional Foods. 2020;64:103692.
doi:10.1016/j.jff.2019.103692 .

Grabež, Milkica, Škrbić, Ranko, Stojiljković, Miloš P., Rudić-Grujić, Vesna, Paunović, Marija P., Arsić, Aleksandra, Petrović, Snježana, Vučić, Vesna M., Mirjanić-Azarić, Bosa, Savikin, Katarina, Menković, Nebojša, Janković, Teodora, Vasiljević, Nada, "Beneficial effects of pomegranate peel extract on plasma lipid profile, fatty acids levels and blood pressure in patients with diabetes mellitus type-2: A randomized, double-blind, placebo-controlled study" in Journal of Functional Foods, 64 (2020):103692,
https://doi.org/10.1016/j.jff.2019.103692 . .