Molecular detection of Babesia spp. in ticks in northern Serbia
2012
Аутори
Mihaljica, DarkoRadulović, Željko
Tomanović, Snežana
Ćakić, Sanja
Penezić, Aleksandra
Milutinović, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.
Кључне речи:
ticks / PCR / Babesia spp. / SerbiaИзвор:
Archives of Biological Sciences, 2012, 64, 4, 1591-1598Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Ензоотски трансмисиони циклуси патогених микроорганизама које преносе крпељи (RS-MESTD-Basic Research (BR or ON)-173006)
DOI: 10.2298/ABS1204591M
ISSN: 0354-4664
WoS: 000312046200043
Scopus: 2-s2.0-84867792020
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Mihaljica, Darko AU - Radulović, Željko AU - Tomanović, Snežana AU - Ćakić, Sanja AU - Penezić, Aleksandra AU - Milutinović, Marija PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/407 AB - In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Molecular detection of Babesia spp. in ticks in northern Serbia EP - 1598 IS - 4 SP - 1591 VL - 64 DO - 10.2298/ABS1204591M ER -
@article{ author = "Mihaljica, Darko and Radulović, Željko and Tomanović, Snežana and Ćakić, Sanja and Penezić, Aleksandra and Milutinović, Marija", year = "2012", abstract = "In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Molecular detection of Babesia spp. in ticks in northern Serbia", pages = "1598-1591", number = "4", volume = "64", doi = "10.2298/ABS1204591M" }
Mihaljica, D., Radulović, Ž., Tomanović, S., Ćakić, S., Penezić, A.,& Milutinović, M.. (2012). Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 64(4), 1591-1598. https://doi.org/10.2298/ABS1204591M
Mihaljica D, Radulović Ž, Tomanović S, Ćakić S, Penezić A, Milutinović M. Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences. 2012;64(4):1591-1598. doi:10.2298/ABS1204591M .
Mihaljica, Darko, Radulović, Željko, Tomanović, Snežana, Ćakić, Sanja, Penezić, Aleksandra, Milutinović, Marija, "Molecular detection of Babesia spp. in ticks in northern Serbia" in Archives of Biological Sciences, 64, no. 4 (2012):1591-1598, https://doi.org/10.2298/ABS1204591M . .