TY  - JOUR
AU  - Lijeskić, Olivera
AU  - Bauman, Neda
AU  - Marković, Miloš
AU  - Srbljanović, Jelena
AU  - Bobić, Branko
AU  - Zlatković, Đorđe
AU  - Štajner, Tijana
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1462
AB  - The aim of this study was to evaluate immunogenicity and longevity of the humoral immune response within six months after the homologous (BNT162b2/BNT162b2) or heterologous (BBIBP-CorV/BNT162b2) third dose, and to assess breakthrough infections among vaccinees during the Omicron wave in Serbia. Serum samples were analyzed at four timepoints: five months after the primary series; three weeks, three months, and six months after the boost. IgG antibodies against the receptor-binding domain of the spike protein were detected using enzyme-linked fluorescence assay. Both homologous (n = 55) and heterologous group (n = 36) showed a highly significant increase in antibody concentrations (p < 0.001) three weeks after the boost. A moderate inverse correlation between the age of recipients and the antibody levels at three weeks post-boost was observed in the homologous group (p = 0.02, r = −0.37), while the same correlation was not significant for heterologous group (p = 0.55, r = −0.15). Heterologous group had significantly higher antibody concentrations than homologous group at three weeks (Median 851.4(IQR 766.6–894.1); 784.3(676.9–847.4); p = 0.03) and three months post-boost (766.6(534.8–798.9); 496.8(361.6–664.0); p < 0.001). However, a significant decline in antibody response over time was noted for both strategies. The overall incidence of breakthrough cases was estimated at 36.36% (20/55) for homologous, and 16.67% (6/36) for heterologous group, but none of them required hospitalization. Although observed incidence in the homologous group was more than double when compared to the heterologous group, this difference was not statistically significant, most likely due to the small sample size. In conclusion, waning immunity after inactivated vaccine can be recovered by BNT162b2 heterologous boost regardless of the age of recipients, and both boost strategies induced potent humoral immune response and protection against severe COVID-19 during the Omicron wave. However, as the observed incidence of breakthrough infections was higher in the homologous group, although non-significant, this finding could indicate an advantage of heterologous approach.
PB  - Elsevier
T2  - Vaccine
T2  - Vaccine
T1  - SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost
EP  - 1672
IS  - 7
SP  - 1665
VL  - 42
DO  - 10.1016/j.vaccine.2024.01.085
ER  - 

@article{
author = "Lijeskić, Olivera and Bauman, Neda and Marković, Miloš and Srbljanović, Jelena and Bobić, Branko and Zlatković, Đorđe and Štajner, Tijana",
year = "2024",
abstract = "The aim of this study was to evaluate immunogenicity and longevity of the humoral immune response within six months after the homologous (BNT162b2/BNT162b2) or heterologous (BBIBP-CorV/BNT162b2) third dose, and to assess breakthrough infections among vaccinees during the Omicron wave in Serbia. Serum samples were analyzed at four timepoints: five months after the primary series; three weeks, three months, and six months after the boost. IgG antibodies against the receptor-binding domain of the spike protein were detected using enzyme-linked fluorescence assay. Both homologous (n = 55) and heterologous group (n = 36) showed a highly significant increase in antibody concentrations (p < 0.001) three weeks after the boost. A moderate inverse correlation between the age of recipients and the antibody levels at three weeks post-boost was observed in the homologous group (p = 0.02, r = −0.37), while the same correlation was not significant for heterologous group (p = 0.55, r = −0.15). Heterologous group had significantly higher antibody concentrations than homologous group at three weeks (Median 851.4(IQR 766.6–894.1); 784.3(676.9–847.4); p = 0.03) and three months post-boost (766.6(534.8–798.9); 496.8(361.6–664.0); p < 0.001). However, a significant decline in antibody response over time was noted for both strategies. The overall incidence of breakthrough cases was estimated at 36.36% (20/55) for homologous, and 16.67% (6/36) for heterologous group, but none of them required hospitalization. Although observed incidence in the homologous group was more than double when compared to the heterologous group, this difference was not statistically significant, most likely due to the small sample size. In conclusion, waning immunity after inactivated vaccine can be recovered by BNT162b2 heterologous boost regardless of the age of recipients, and both boost strategies induced potent humoral immune response and protection against severe COVID-19 during the Omicron wave. However, as the observed incidence of breakthrough infections was higher in the homologous group, although non-significant, this finding could indicate an advantage of heterologous approach.",
publisher = "Elsevier",
journal = "Vaccine, Vaccine",
title = "SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost",
pages = "1672-1665",
number = "7",
volume = "42",
doi = "10.1016/j.vaccine.2024.01.085"
}

Lijeskić, O., Bauman, N., Marković, M., Srbljanović, J., Bobić, B., Zlatković, Đ.,& Štajner, T.. (2024). SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost. in Vaccine
Elsevier., 42(7), 1665-1672.
https://doi.org/10.1016/j.vaccine.2024.01.085

Lijeskić O, Bauman N, Marković M, Srbljanović J, Bobić B, Zlatković Đ, Štajner T. SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost. in Vaccine. 2024;42(7):1665-1672.
doi:10.1016/j.vaccine.2024.01.085 .

Lijeskić, Olivera, Bauman, Neda, Marković, Miloš, Srbljanović, Jelena, Bobić, Branko, Zlatković, Đorđe, Štajner, Tijana, "SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost" in Vaccine, 42, no. 7 (2024):1665-1672,
https://doi.org/10.1016/j.vaccine.2024.01.085 . .

TY  - JOUR
AU  - Bauman, Neda
AU  - Srbljanović, Jelena
AU  - Čolović Čalovski, Ivana
AU  - Lijeskić, Olivera
AU  - Ćirković, Vladimir
AU  - Trajković, Jelena
AU  - Bobić, Branko
AU  - Ilić, Andjelija Ž
AU  - Štajner, Tijana
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1473
AB  - Toxoplasma gondii is an obligate intracellular parasite existing in three infectious life stages—tachyzoites, bradyzoites, and sporozoites. Rupture of tissue cysts and re-conversion of bradyzoites to tachyzoites leads to reactivated toxoplasmosis (RT) in an immunocompromised host. The aim of this study was to apply ImageJ software for analysis of T. gondii brain cysts obtained from a newly established in vivo model of RT. Mice chronically infected with T. gondii (BGD1 and BGD26 strains) were treated with cyclophosphamide and hydrocortisone (experimental group—EG) or left untreated as infection controls (ICs). RT in mice was confirmed by qPCR (PCR+); mice remaining chronically infected were PCR−. A total of 90 images of cysts were analyzed for fractal dimension (FD), lacunarity (L), diameter (D), circularity (C), and packing density (PD). Circularity was significantly higher in PCR+ compared to IC mice (p < 0.05 for BGD1, p < 0.001 for the BGD26 strain). A significant negative correlation between D and PD was observed only in IC for the BGD1 strain (ρ = −0.384, p = 0.048), while fractal parameters were stable. Significantly higher D, C, and PD and lower lacunarity, L, were noticed in the BGD1 compared to the more aggressive BGD26 strain. In conclusion, these results demonstrate the complexity of structural alterations of T. gondii cysts in an immunocompromised host and emphasize the application potential of ImageJ in the experimental models of toxoplasmosis.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Fractal and Fractional
T2  - Fractal and Fractional
T1  - Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis
IS  - 3
SP  - 175
VL  - 8
DO  - 10.3390/fractalfract8030175
ER  - 

@article{
author = "Bauman, Neda and Srbljanović, Jelena and Čolović Čalovski, Ivana and Lijeskić, Olivera and Ćirković, Vladimir and Trajković, Jelena and Bobić, Branko and Ilić, Andjelija Ž and Štajner, Tijana",
year = "2024",
abstract = "Toxoplasma gondii is an obligate intracellular parasite existing in three infectious life stages—tachyzoites, bradyzoites, and sporozoites. Rupture of tissue cysts and re-conversion of bradyzoites to tachyzoites leads to reactivated toxoplasmosis (RT) in an immunocompromised host. The aim of this study was to apply ImageJ software for analysis of T. gondii brain cysts obtained from a newly established in vivo model of RT. Mice chronically infected with T. gondii (BGD1 and BGD26 strains) were treated with cyclophosphamide and hydrocortisone (experimental group—EG) or left untreated as infection controls (ICs). RT in mice was confirmed by qPCR (PCR+); mice remaining chronically infected were PCR−. A total of 90 images of cysts were analyzed for fractal dimension (FD), lacunarity (L), diameter (D), circularity (C), and packing density (PD). Circularity was significantly higher in PCR+ compared to IC mice (p < 0.05 for BGD1, p < 0.001 for the BGD26 strain). A significant negative correlation between D and PD was observed only in IC for the BGD1 strain (ρ = −0.384, p = 0.048), while fractal parameters were stable. Significantly higher D, C, and PD and lower lacunarity, L, were noticed in the BGD1 compared to the more aggressive BGD26 strain. In conclusion, these results demonstrate the complexity of structural alterations of T. gondii cysts in an immunocompromised host and emphasize the application potential of ImageJ in the experimental models of toxoplasmosis.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Fractal and Fractional, Fractal and Fractional",
title = "Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis",
number = "3",
pages = "175",
volume = "8",
doi = "10.3390/fractalfract8030175"
}

Bauman, N., Srbljanović, J., Čolović Čalovski, I., Lijeskić, O., Ćirković, V., Trajković, J., Bobić, B., Ilić, A. Ž.,& Štajner, T.. (2024). Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis. in Fractal and Fractional
Multidisciplinary Digital Publishing Institute (MDPI)., 8(3), 175.
https://doi.org/10.3390/fractalfract8030175

Bauman N, Srbljanović J, Čolović Čalovski I, Lijeskić O, Ćirković V, Trajković J, Bobić B, Ilić AŽ, Štajner T. Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis. in Fractal and Fractional. 2024;8(3):175.
doi:10.3390/fractalfract8030175 .

Bauman, Neda, Srbljanović, Jelena, Čolović Čalovski, Ivana, Lijeskić, Olivera, Ćirković, Vladimir, Trajković, Jelena, Bobić, Branko, Ilić, Andjelija Ž, Štajner, Tijana, "Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis" in Fractal and Fractional, 8, no. 3 (2024):175,
https://doi.org/10.3390/fractalfract8030175 . .

TY  - JOUR
AU  - Marković-Denić, Ljiljana
AU  - Stopić, Milena
AU  - Bobić, Branko
AU  - Nikolić, Vladimir
AU  - Đilas, Iva
AU  - Jovanović Srzentić, Snežana
AU  - Štajner, Tijana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1359
AB  - Toxoplasmosis, caused by the cosmopolitan protozoan Toxoplasma gondii, has particular implications during pregnancy due to the possible transmission of infection to the fetus. Very few studies have assessed seroprevalence and the risk factors for toxoplasmosis in healthy pregnant women. The aim of this study was to examine the seroprevalence of T. gondii infection in healthy pregnant women and to identify the associated risk factors for toxoplasmosis. The cross-sectional study involved 300 healthy pregnant women who came to the Institute for Blood Transfusion in Belgrade between November 2018 and February 2019 for routine blood group and Rh factor testing before delivery, who were also tested using serological screening for the presence of specific antibodies. Positives were further examined using enzyme immunoassay. Of the total sera of participants analyzed, 38 were positive for specific IgG, resulting in a seroprevalence rate of 12.7% (95% Confidence Interval (CI) 9.1–17.0%). All pregnant women presented negative anti-T. gondii IgM antibodies. The multivariate logistic regression analysis revealed that living in a house with a garden was independently associated with the risk of T. gondii infections, while eating chicken meat was connected with a lower risk compared to eating other types of meat with an odds ratio (OR) of 2.5 (95% CI 1.21–5.02) and an OR of 0.3 (95% CI 0.09–0.83), respectively. Although the prevalence of anti-T. gondii IgG antibodies is relatively low, it is essential to maintain and adapt evidence-based preventive measures for toxoplasmosis continually.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Pathogens
T2  - Pathogens
T1  - Factors Associated with Toxoplasma gondii Seroprevalence in Pregnant Women: A Cross-Sectional Study in Belgrade, Serbia
IS  - 10
SP  - 1240
VL  - 12
DO  - 10.3390/pathogens12101240
ER  - 

@article{
author = "Marković-Denić, Ljiljana and Stopić, Milena and Bobić, Branko and Nikolić, Vladimir and Đilas, Iva and Jovanović Srzentić, Snežana and Štajner, Tijana",
year = "2023",
abstract = "Toxoplasmosis, caused by the cosmopolitan protozoan Toxoplasma gondii, has particular implications during pregnancy due to the possible transmission of infection to the fetus. Very few studies have assessed seroprevalence and the risk factors for toxoplasmosis in healthy pregnant women. The aim of this study was to examine the seroprevalence of T. gondii infection in healthy pregnant women and to identify the associated risk factors for toxoplasmosis. The cross-sectional study involved 300 healthy pregnant women who came to the Institute for Blood Transfusion in Belgrade between November 2018 and February 2019 for routine blood group and Rh factor testing before delivery, who were also tested using serological screening for the presence of specific antibodies. Positives were further examined using enzyme immunoassay. Of the total sera of participants analyzed, 38 were positive for specific IgG, resulting in a seroprevalence rate of 12.7% (95% Confidence Interval (CI) 9.1–17.0%). All pregnant women presented negative anti-T. gondii IgM antibodies. The multivariate logistic regression analysis revealed that living in a house with a garden was independently associated with the risk of T. gondii infections, while eating chicken meat was connected with a lower risk compared to eating other types of meat with an odds ratio (OR) of 2.5 (95% CI 1.21–5.02) and an OR of 0.3 (95% CI 0.09–0.83), respectively. Although the prevalence of anti-T. gondii IgG antibodies is relatively low, it is essential to maintain and adapt evidence-based preventive measures for toxoplasmosis continually.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Pathogens, Pathogens",
title = "Factors Associated with Toxoplasma gondii Seroprevalence in Pregnant Women: A Cross-Sectional Study in Belgrade, Serbia",
number = "10",
pages = "1240",
volume = "12",
doi = "10.3390/pathogens12101240"
}

Marković-Denić, L., Stopić, M., Bobić, B., Nikolić, V., Đilas, I., Jovanović Srzentić, S.,& Štajner, T.. (2023). Factors Associated with Toxoplasma gondii Seroprevalence in Pregnant Women: A Cross-Sectional Study in Belgrade, Serbia. in Pathogens
Multidisciplinary Digital Publishing Institute (MDPI)., 12(10), 1240.
https://doi.org/10.3390/pathogens12101240

Marković-Denić L, Stopić M, Bobić B, Nikolić V, Đilas I, Jovanović Srzentić S, Štajner T. Factors Associated with Toxoplasma gondii Seroprevalence in Pregnant Women: A Cross-Sectional Study in Belgrade, Serbia. in Pathogens. 2023;12(10):1240.
doi:10.3390/pathogens12101240 .

Marković-Denić, Ljiljana, Stopić, Milena, Bobić, Branko, Nikolić, Vladimir, Đilas, Iva, Jovanović Srzentić, Snežana, Štajner, Tijana, "Factors Associated with Toxoplasma gondii Seroprevalence in Pregnant Women: A Cross-Sectional Study in Belgrade, Serbia" in Pathogens, 12, no. 10 (2023):1240,
https://doi.org/10.3390/pathogens12101240 . .

TY  - CONF
AU  - Štajner, Tijana
AU  - Vujić, Dragana
AU  - Nestorović, Emilija
AU  - Srbljanović, Jelena
AU  - Bauman, Neda
AU  - Lijeskić, Olivera
AU  - Zečević, Željko
AU  - Simić, Marija
AU  - Terzić, Duško
AU  - Jovanović, Snežana
AU  - Dakić, Zorica
AU  - Bobić, Branko
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1450
AB  - Toksoplazmoza je česta ali kod pacijenata lečenih transplantacijom uglavnom zanemarena i pogrešno
dijagnostikovana oportunistička infekcija koja može ugroziti engraftment ali može i evoluirati u
životno ugrožavajuću diseminovanu infekciju. Nakon transplantacije, infekcija parazitom Toxoplasma
gondii se može razviti kao reaktivacija hronične infekcije ili može biti preneta graftom.
Naša osmogodišnja prospektivna studija bila je usmerena na dijagnostiku i monitoring toksoplazmatske
infekcije (TI) kod primalaca matičnih ćelija hematopoeze (haematopoietic stem cell
transplant, HSCT) u centru koji primenjuje protokol uzdržavanja od profilakse do engraftmenta, i
kod primalaca transplantata srca (heart transplant, HT) koji su na kontinuiranoj profilaksi trimetoprim-
sulfametoksazolom (TMP-SMX).
Cilj nam je bio utvrđivanje incidence TI u ova dva vrlo različita transplantaciona režima, i to pre nego
što evoluira u klinički manifestnu, potencijalno fatalnu bolest (Toxoplasma disease, TD). Pre-transplantacioni
serološki i qPCR skrining u post-transplantacionom toku zamenjen je redovnim qPCR
monitoringom iz uzoraka periferne krvi (peripheral blood, PB) usmerenim na Toxoplasma 529 bp gen. Kod primalaca HSCT, qPCR je rađen jednom nedeljno dok je kod primalaca HT qPCR rađen jednom
mesečno prva dva meseca post-HT i potom jednom godišnje. TI je dijagnostikovana na bazi
pozitivnog PCR rezultata iz bar jednog uzorka PB. TI je dijagnostikovana kod 21/104 (20.2%) primalaca
HSCT, prevashodno nakon alogene (19/75) i retko nakon autologne HSCT (2/29). Više od
50% slučajeva TI dijagnostikovano je tokom prvog meseca post-HSCT, pre engraftmenta odnosno
tokom uzdržavanja od profilakse. Sa druge strane, TI je dijagnostikovana kod 3/37 (8.1%) primalaca
HT. Uprkos primeni TMP-SMX, qPCR je postao pozitivan godinu dana posle HT kod dva i dve godine
post-HSCT kod trećeg pacijenta. Infekcija je bila preneta graftom kod 2/3 (seronegativni) a reaktivirana
kod 1/3 primalaca HT (seropozitivni primalac HT poreklom od seropozitivnog donora).
Naši rezultati potvrđuju da je sistemski qPCR monitoring iz uzoraka PB dragocen u dijagnostici TI
ne samo kod primalaca HSCT već i kod primalaca solidnih organa, posebno nakon HT. Učestalost
qPCR monitoringa se mora adaptirati shodno specifičnostima transplantacionog protokola, pre
svega primeni profilakse ali i osnovnoj dijagnozi, na način koji omogućava pravovremenu primenu
specifične terapije u svakom slučaju TI.
AB  - Toxoplasmosis is a common but often neglected and misdiagnosed opportunistic infection in transplant
recipients, which can not only compromise the engraftment, but also evolve into life-threatening
disseminated infection. Post-transplantation, Toxoplasma gondii infection can develop as a reactivation
of chronic infection or could be graft-transmitted. We conducted an eight-year-long prospective
study on the diagnosis and monitoring of Toxoplasma infection (TI) in haematopoietic stem cell
transplant (HSCT) recipients in a setting that withholds prophylaxis until engraftment, and in heart
transplant (HT) recipients on continuous trimethoprim-sulfamethoxazole (TMP-SMX) prophylaxis.
The objective was to determine the incidence of TI before it evolves into clinical, potentially fatal
Toxoplasma disease (TD), in these two very different transplantation settings. Pre-transplantation
serological and qPCR screening was followed by post-transplantation peripheral blood (PB)-based
qPCR monitoring targeting the Toxoplasma 529 bp gene. In HSCT recipients, qPCR was performed
weekly while in HT recipients, qPCR was performed monthly for two months post-HT and then
yearly. TI was diagnosed based on a positive PCR result in at least one PB sample.
TI was diagnosed in 21/104 (20.2%) HSCT recipients, predominantly after allogeneic (19/75) and
rarely after autologous HSCT (2/29). Over 50% of TI cases were diagnosed during the first month
post-HSCT, while awaiting engraftment without prophylaxis. On the other hand, TI was diagnosed
in 3/37 (8.1%) HT recipients. Regardless of the TMP-SMX prophylaxis, qPCR became positive one
year after HT in two and two years post-HSCT in third patient. Infection was graft-transmitted in 2/3
(seronegative) and reactivated in 1/3 OHT (seropositive recipient of a seropositive donor’s heart
transplant).
The presented results show that systematic PB-based qPCR monitoring is a valuable resource for
the diagnosis of TI not only in HSCT but also in solid organ recipients, especially after HT. Frequency
of qPCR monitoring should be adjusted according to the specificity of the transplantation setting,
especially in terms of prophylaxis but also an underlying diagnosis, in a manner allowing for prompt
introduction of specific treatment in each case of TI.
PB  - Belgrade: Serbian Society for Microbiology
C3  - 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
T1  - Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije
T1  - Transplantation-related risk of Toxoplasma gondii infection: the National Reference Laboratory prospective cohort study results
EP  - 73
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1450
ER  - 

@conference{
author = "Štajner, Tijana and Vujić, Dragana and Nestorović, Emilija and Srbljanović, Jelena and Bauman, Neda and Lijeskić, Olivera and Zečević, Željko and Simić, Marija and Terzić, Duško and Jovanović, Snežana and Dakić, Zorica and Bobić, Branko",
year = "2023",
abstract = "Toksoplazmoza je česta ali kod pacijenata lečenih transplantacijom uglavnom zanemarena i pogrešno
dijagnostikovana oportunistička infekcija koja može ugroziti engraftment ali može i evoluirati u
životno ugrožavajuću diseminovanu infekciju. Nakon transplantacije, infekcija parazitom Toxoplasma
gondii se može razviti kao reaktivacija hronične infekcije ili može biti preneta graftom.
Naša osmogodišnja prospektivna studija bila je usmerena na dijagnostiku i monitoring toksoplazmatske
infekcije (TI) kod primalaca matičnih ćelija hematopoeze (haematopoietic stem cell
transplant, HSCT) u centru koji primenjuje protokol uzdržavanja od profilakse do engraftmenta, i
kod primalaca transplantata srca (heart transplant, HT) koji su na kontinuiranoj profilaksi trimetoprim-
sulfametoksazolom (TMP-SMX).
Cilj nam je bio utvrđivanje incidence TI u ova dva vrlo različita transplantaciona režima, i to pre nego
što evoluira u klinički manifestnu, potencijalno fatalnu bolest (Toxoplasma disease, TD). Pre-transplantacioni
serološki i qPCR skrining u post-transplantacionom toku zamenjen je redovnim qPCR
monitoringom iz uzoraka periferne krvi (peripheral blood, PB) usmerenim na Toxoplasma 529 bp gen. Kod primalaca HSCT, qPCR je rađen jednom nedeljno dok je kod primalaca HT qPCR rađen jednom
mesečno prva dva meseca post-HT i potom jednom godišnje. TI je dijagnostikovana na bazi
pozitivnog PCR rezultata iz bar jednog uzorka PB. TI je dijagnostikovana kod 21/104 (20.2%) primalaca
HSCT, prevashodno nakon alogene (19/75) i retko nakon autologne HSCT (2/29). Više od
50% slučajeva TI dijagnostikovano je tokom prvog meseca post-HSCT, pre engraftmenta odnosno
tokom uzdržavanja od profilakse. Sa druge strane, TI je dijagnostikovana kod 3/37 (8.1%) primalaca
HT. Uprkos primeni TMP-SMX, qPCR je postao pozitivan godinu dana posle HT kod dva i dve godine
post-HSCT kod trećeg pacijenta. Infekcija je bila preneta graftom kod 2/3 (seronegativni) a reaktivirana
kod 1/3 primalaca HT (seropozitivni primalac HT poreklom od seropozitivnog donora).
Naši rezultati potvrđuju da je sistemski qPCR monitoring iz uzoraka PB dragocen u dijagnostici TI
ne samo kod primalaca HSCT već i kod primalaca solidnih organa, posebno nakon HT. Učestalost
qPCR monitoringa se mora adaptirati shodno specifičnostima transplantacionog protokola, pre
svega primeni profilakse ali i osnovnoj dijagnozi, na način koji omogućava pravovremenu primenu
specifične terapije u svakom slučaju TI., Toxoplasmosis is a common but often neglected and misdiagnosed opportunistic infection in transplant
recipients, which can not only compromise the engraftment, but also evolve into life-threatening
disseminated infection. Post-transplantation, Toxoplasma gondii infection can develop as a reactivation
of chronic infection or could be graft-transmitted. We conducted an eight-year-long prospective
study on the diagnosis and monitoring of Toxoplasma infection (TI) in haematopoietic stem cell
transplant (HSCT) recipients in a setting that withholds prophylaxis until engraftment, and in heart
transplant (HT) recipients on continuous trimethoprim-sulfamethoxazole (TMP-SMX) prophylaxis.
The objective was to determine the incidence of TI before it evolves into clinical, potentially fatal
Toxoplasma disease (TD), in these two very different transplantation settings. Pre-transplantation
serological and qPCR screening was followed by post-transplantation peripheral blood (PB)-based
qPCR monitoring targeting the Toxoplasma 529 bp gene. In HSCT recipients, qPCR was performed
weekly while in HT recipients, qPCR was performed monthly for two months post-HT and then
yearly. TI was diagnosed based on a positive PCR result in at least one PB sample.
TI was diagnosed in 21/104 (20.2%) HSCT recipients, predominantly after allogeneic (19/75) and
rarely after autologous HSCT (2/29). Over 50% of TI cases were diagnosed during the first month
post-HSCT, while awaiting engraftment without prophylaxis. On the other hand, TI was diagnosed
in 3/37 (8.1%) HT recipients. Regardless of the TMP-SMX prophylaxis, qPCR became positive one
year after HT in two and two years post-HSCT in third patient. Infection was graft-transmitted in 2/3
(seronegative) and reactivated in 1/3 OHT (seropositive recipient of a seropositive donor’s heart
transplant).
The presented results show that systematic PB-based qPCR monitoring is a valuable resource for
the diagnosis of TI not only in HSCT but also in solid organ recipients, especially after HT. Frequency
of qPCR monitoring should be adjusted according to the specificity of the transplantation setting,
especially in terms of prophylaxis but also an underlying diagnosis, in a manner allowing for prompt
introduction of specific treatment in each case of TI.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book",
title = "Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije, Transplantation-related risk of Toxoplasma gondii infection: the National Reference Laboratory prospective cohort study results",
pages = "73-70",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1450"
}

Štajner, T., Vujić, D., Nestorović, E., Srbljanović, J., Bauman, N., Lijeskić, O., Zečević, Ž., Simić, M., Terzić, D., Jovanović, S., Dakić, Z.,& Bobić, B.. (2023). Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
Belgrade: Serbian Society for Microbiology., 70-73.
https://hdl.handle.net/21.15107/rcub_rimi_1450

Štajner T, Vujić D, Nestorović E, Srbljanović J, Bauman N, Lijeskić O, Zečević Ž, Simić M, Terzić D, Jovanović S, Dakić Z, Bobić B. Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book. 2023;:70-73.
https://hdl.handle.net/21.15107/rcub_rimi_1450 .

Štajner, Tijana, Vujić, Dragana, Nestorović, Emilija, Srbljanović, Jelena, Bauman, Neda, Lijeskić, Olivera, Zečević, Željko, Simić, Marija, Terzić, Duško, Jovanović, Snežana, Dakić, Zorica, Bobić, Branko, "Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije" in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book (2023):70-73,
https://hdl.handle.net/21.15107/rcub_rimi_1450 .

TY  - CONF
AU  - Lijeskić, Olivera
AU  - Srbljanović, Jelena
AU  - Bauman, Neda
AU  - Bobić, Branko
AU  - Štajner, Tijana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1452
AB  - Implementacija treće doze vakcine protiv SARS-CoV-2 u preporuke širom sveta otvorila je polje
istraživanja heterologog pristupa revakcinaciji, odnosno kombinacije primarne serije vakcine i treće
doze različite vakcinalne platforme. Iako je literatura bogata radovima na temu heterologog pristupa,
imunogenost i trajanje humoralnog imunskog odgovora nakon kombinacije inaktivisane BBIBPCorV
i iRNK vakcine nisu dovoljno istraženi. Stoga, cilj ove studije bio je ispitivanje razlike u imunogenosti
i dugotrajnosti humoralnog imunskog odgovora u okviru perioda od šest meseci nakon
treće doze kod homologog (tri doze BNT162b2) i heterologog (BBIBP-CorV/BNT162b2) pristupa
revakcinaciji tokom Omikron talasa u Srbiji. U studiju je uključen 91 ispitanik, od kojih se 55 odlučilo
za homologi a 36 za heterologi pristup.
Serumi ispitanika analizirani su u četiri vremenske tačke: šest meseci nakon prve doze, a zatim tri nedelje,
tri meseca i šest meseci nakon treće doze. IgG antitela specifična za receptor-vezujući domen “šiljastog”
(eng. spike) proteina detektovana su BioMerieux VIDAS SARS-CoV-2 IgG testom. Tri nedelje
nakon treće doze, oba pristupa revakcinaciji dovela su do značajnog porasta u koncentraciji antitela
(p<0.0001). Štaviše, ispitanici koji su se opredelili za heterologu kombinaciju imali su statistički
značajno više koncentracije antitela od homologe grupe, u kontrolnim vremenskim tačkama na tri
nedelje i tri meseca nakon treće doze (p=0.025, p=0.0006). Međutim, značajan pad humoralnog
imunskog odgovora zapažen je tokom vremena kod oba pristupa. Većina infekcija nakon vakcinacije
registrovana je u periodu između tri i šest meseci nakon treće doze (n=22), a ukupna incidencija
ovih infekcija za posmatrani period iznosila je 36.36% (20/55) nakon homologog i 16.67% (6/36)
nakon heterologog pristupa.
Međutim, ispitanici sa potvrđenom infekcijom nakon vakcinacije nisu imali pneumoniju niti su bili
hospitalizovani. Iako je heterologi pristup indukovao više koncentracije antitela, naši rezultati ukazuju
da su i heterologi i homologi pristup indukovali potentan humoralni imunski odgovor i odgovarajuću
zaštitu od hospitalizacije i smrtnog ishoda tokom Omikron talasa. Međutim, opadanje imunskog
odgovora opaženo kod oba vakcinalna pristupa u periodu od šest meseci, kao i konstantna opasnost
od pojave novih pretećih varijanti, ukazuje na potrebu preispitivanja trenutne vakcinalne strategije.
AB  - Worldwide implementation of the third dose of vaccine against SARS-CoV-2 opened a new field of
research concerning the heterologous boost i.e., the combination of the primary vaccine series and
a different vaccinal platform for the third dose. Although literature is replete with studies of heterologous
boosts, longevity and immunogenicity of the inactivated BBIBP-CorV and mRNA BNT162b2
combination remains under-explored. Thus, the aim of this study was to evaluate the differences in
immunogenicity and longevity of the humoral immune response within six months after the third
dose in both homologous (BNT162b2) and heterologous (BBIBP-CorV/BNT162b2) vaccination setting,
and to assess the real-life data in the middle of the Omicron surge in Serbia.
A total of 91 individuals were included in this study, of which 55 received homologous and 36 heterologous
boost. Serum samples were analyzed at four timepoints: six months after the first dose;
three weeks, three months, and six months after the third dose. Specific IgG antibodies against the
receptor-binding domain of the spike protein were detected using BioMerieux VIDAS SARS-CoV-2
IgG kit. Both groups showed a highly significant increase in antibody concentrations (p<0.0001)
three weeks after the boost.
Furthermore, comparison per timepoint has shown that recipients of heterologous boost had significantly
higher antibody concentrations than homologous group, at three weeks and three months
after the boost (p=0.025, p=0.0006). However, a significant decline in antibody response over time
was noted for both strategies. The majority of breakthrough infections were registered in the period
between three and six months after the boost (n=22).Furthermore, total incidence was estimated at 36.36% (20/55) for homologous group, and 16.67%
(6/36) for heterologous group. Most importantly, none of the recipients of the third dose developed
pneumonia during the breakthrough infection, and none were hospitalized. In conclusion, although
heterologous approach resulted in higher antibody concentrations, our findings imply that both
homologous and heterologous boost induce potent humoral immune response and adequate protection
against hospitalization and death in the Omicron setting. However, waning immune
response registered for both types of boosts within six months and constant threats of new emerging
variants, calls for an update of vaccine strategy.
PB  - Belgrade: Serbian Society for Microbiology
C3  - 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
T1  - Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji
T1  - SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: homologous versus heterologous boost
EP  - 82
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1452
ER  - 

@conference{
author = "Lijeskić, Olivera and Srbljanović, Jelena and Bauman, Neda and Bobić, Branko and Štajner, Tijana",
year = "2023",
abstract = "Implementacija treće doze vakcine protiv SARS-CoV-2 u preporuke širom sveta otvorila je polje
istraživanja heterologog pristupa revakcinaciji, odnosno kombinacije primarne serije vakcine i treće
doze različite vakcinalne platforme. Iako je literatura bogata radovima na temu heterologog pristupa,
imunogenost i trajanje humoralnog imunskog odgovora nakon kombinacije inaktivisane BBIBPCorV
i iRNK vakcine nisu dovoljno istraženi. Stoga, cilj ove studije bio je ispitivanje razlike u imunogenosti
i dugotrajnosti humoralnog imunskog odgovora u okviru perioda od šest meseci nakon
treće doze kod homologog (tri doze BNT162b2) i heterologog (BBIBP-CorV/BNT162b2) pristupa
revakcinaciji tokom Omikron talasa u Srbiji. U studiju je uključen 91 ispitanik, od kojih se 55 odlučilo
za homologi a 36 za heterologi pristup.
Serumi ispitanika analizirani su u četiri vremenske tačke: šest meseci nakon prve doze, a zatim tri nedelje,
tri meseca i šest meseci nakon treće doze. IgG antitela specifična za receptor-vezujući domen “šiljastog”
(eng. spike) proteina detektovana su BioMerieux VIDAS SARS-CoV-2 IgG testom. Tri nedelje
nakon treće doze, oba pristupa revakcinaciji dovela su do značajnog porasta u koncentraciji antitela
(p<0.0001). Štaviše, ispitanici koji su se opredelili za heterologu kombinaciju imali su statistički
značajno više koncentracije antitela od homologe grupe, u kontrolnim vremenskim tačkama na tri
nedelje i tri meseca nakon treće doze (p=0.025, p=0.0006). Međutim, značajan pad humoralnog
imunskog odgovora zapažen je tokom vremena kod oba pristupa. Većina infekcija nakon vakcinacije
registrovana je u periodu između tri i šest meseci nakon treće doze (n=22), a ukupna incidencija
ovih infekcija za posmatrani period iznosila je 36.36% (20/55) nakon homologog i 16.67% (6/36)
nakon heterologog pristupa.
Međutim, ispitanici sa potvrđenom infekcijom nakon vakcinacije nisu imali pneumoniju niti su bili
hospitalizovani. Iako je heterologi pristup indukovao više koncentracije antitela, naši rezultati ukazuju
da su i heterologi i homologi pristup indukovali potentan humoralni imunski odgovor i odgovarajuću
zaštitu od hospitalizacije i smrtnog ishoda tokom Omikron talasa. Međutim, opadanje imunskog
odgovora opaženo kod oba vakcinalna pristupa u periodu od šest meseci, kao i konstantna opasnost
od pojave novih pretećih varijanti, ukazuje na potrebu preispitivanja trenutne vakcinalne strategije., Worldwide implementation of the third dose of vaccine against SARS-CoV-2 opened a new field of
research concerning the heterologous boost i.e., the combination of the primary vaccine series and
a different vaccinal platform for the third dose. Although literature is replete with studies of heterologous
boosts, longevity and immunogenicity of the inactivated BBIBP-CorV and mRNA BNT162b2
combination remains under-explored. Thus, the aim of this study was to evaluate the differences in
immunogenicity and longevity of the humoral immune response within six months after the third
dose in both homologous (BNT162b2) and heterologous (BBIBP-CorV/BNT162b2) vaccination setting,
and to assess the real-life data in the middle of the Omicron surge in Serbia.
A total of 91 individuals were included in this study, of which 55 received homologous and 36 heterologous
boost. Serum samples were analyzed at four timepoints: six months after the first dose;
three weeks, three months, and six months after the third dose. Specific IgG antibodies against the
receptor-binding domain of the spike protein were detected using BioMerieux VIDAS SARS-CoV-2
IgG kit. Both groups showed a highly significant increase in antibody concentrations (p<0.0001)
three weeks after the boost.
Furthermore, comparison per timepoint has shown that recipients of heterologous boost had significantly
higher antibody concentrations than homologous group, at three weeks and three months
after the boost (p=0.025, p=0.0006). However, a significant decline in antibody response over time
was noted for both strategies. The majority of breakthrough infections were registered in the period
between three and six months after the boost (n=22).Furthermore, total incidence was estimated at 36.36% (20/55) for homologous group, and 16.67%
(6/36) for heterologous group. Most importantly, none of the recipients of the third dose developed
pneumonia during the breakthrough infection, and none were hospitalized. In conclusion, although
heterologous approach resulted in higher antibody concentrations, our findings imply that both
homologous and heterologous boost induce potent humoral immune response and adequate protection
against hospitalization and death in the Omicron setting. However, waning immune
response registered for both types of boosts within six months and constant threats of new emerging
variants, calls for an update of vaccine strategy.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book",
title = "Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji, SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: homologous versus heterologous boost",
pages = "82-79",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1452"
}

Lijeskić, O., Srbljanović, J., Bauman, N., Bobić, B.,& Štajner, T.. (2023). Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
Belgrade: Serbian Society for Microbiology., 79-82.
https://hdl.handle.net/21.15107/rcub_rimi_1452

Lijeskić O, Srbljanović J, Bauman N, Bobić B, Štajner T. Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book. 2023;:79-82.
https://hdl.handle.net/21.15107/rcub_rimi_1452 .

Lijeskić, Olivera, Srbljanović, Jelena, Bauman, Neda, Bobić, Branko, Štajner, Tijana, "Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji" in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book (2023):79-82,
https://hdl.handle.net/21.15107/rcub_rimi_1452 .

TY  - CONF
AU  - Srbljanović, Jelena
AU  - Štajner, Tijana
AU  - Bauman, Neda
AU  - Lijeskić, Olivera
AU  - Opsenica, Igor
AU  - Šolaja, Bogdan
AU  - Bobić, Branko
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1453
AB  - With an estimated 247 million cases annually and 619.000 deaths (in 2021) malaria remains a major
disease of the developing world and globally the most important parasitic disease. Because of
widespread resistance to available antimalarials including chloroquine (CQ) and its derivatives, new
drugs are urgently needed. Here we report on the antimalarial efficacy of new 4-aminoquinoline
derivatives, with modifications at the linker and at the quinoline nucleus.
In vitro screening was performed by the lactate dehydrogenase assay, based on measurement of the
plasmodial lactate dehydrogenase activity in both a CQ-sensitive (3D7) and a CQ-resistant (Dd2)
strain of Plasmodium falciparum, with a CQ as a control. In vivo antimalarial activity was investigated
in C57BL/6 mice infected with Plasmodium berghei ANKA strain by the modified Thompson test.
Compounds were first tested for toxicity. A total of 37 compounds were screened in vitro. Of the 22
that passed the first screening, 18 had IC50 values lower than CQ in the Dd2 strain while only one
was efficient in the 3D7 strain. However, even 15 compounds showed in vivo activity, significantly
(P<0.05) prolonging survival of treated vs. untreated mice. Among these, seven compounds afforded
the survival of 20–100% of treated mice up to Day 31, with or without the detection of parasites
in peripheral blood.
Most importantly, three of these, including ClAQ1, FClAQ1 and ClAQ8, afforded survival of 100% of
animals, the first two at 80 and 160 mg/kg/day and the last only at 160 mg/kg/day.Survival was associated with complete parasite clearance, as shown by both microscopy and qPCR.
Of note, continuous monitoring of parasitemia allowed the observation of a potentially important
phenomenon, that a number of compounds were able to confer resistance to cerebral malaria and
afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.
By comparing the antimalarial activity of this group of novel compounds, we found that even minor
structural modifications substantially affect activity. The results of this extensive study are important,
as they may guide future work involving structural modifications of aminoquinolines, and as a contribution
to the knowledge in the field of malarial chemotherapy.
AB  - Malarija ostaje globalno najznačajnija parazitska infekcija sa procenjenih 247 miliona slučajeva
i 619.000 smrtnih slučajeva godišnje (2021.). Zbog široko rasprostranjene rezistencije na dostupne
antimalarike, uključujući hlorokvin (CQ) i njegove derivate, hitno su potrebni novi lekovi.
U ovom istraživanju ispitana je potencijalna antimalarijska aktivnost 37 novosintetisanih aminohinolina
sa hemijskim modifikacijama na aminohinolinskom jezgru i bočnom lancu. In vitro skrining aktivnosti
jedinjenja vršen je kolorimetrijskim esejom laktat dehidrogenaze na dva soja Plasmodium falciparum,
osetljivim (3D7) i rezistentnim (Dd2) na CQ, uz CQ kao pozitivnu kontrolu. Aktivnost u in
vivo sistemu je ispitana na ženkama miševa soja C57Bl/6 inficiranim ANKA sojem Plasmodium berghei
primenom modifikovanog Thompson-ovog testa. Ispitivanju aktivnosti jedinjenja prethodila je
faza kliničkog praćenja zdravih životinja terapiranih eksperimentalnim jedinjenjima. Od 37 jedinjenja
ispitanih u fazi in vitro skrininga, 22 koja su inhibirala ≥50% rast bar jednog od dva soja P. falciparum
odabrana su za titraciju do IC50 vrednosti.
Prema soju rezistentnom na CQ, 18 jedinjenja se pokazalo aktivnijim od CQ, dok je među njima samo
jedno jedinjenje bilo aktivnije i prema osetljivom soju. Čak 15 jedinjenja ispitanih u in vivo sistemu
značajno je produžilo život inficiranim životinjama u odnosu na kontrolnu grupu (P < 0.05).
Među njima, sedam jedinjenja je omogućilo preživljavanje 20–100% tretiranih miševa do dana 31, sa
ili bez nalaza parazita u perifernoj krvi. Posebno treba istaći tri jedinjenja koja su dovela do izlečenja
svih tretiranih životinja, ClAQ1 i FClAQ1 (80 i 160 mg/kg/dan) i ClAQ8 (160 mg/kg/dan).Preživljavanje je bilo praćeno i kompletnim klirensom parazita što je dokazano mikroskopskim pregledom
razmaza kao i qPCR analizom krvi i tkiva jetre preživelih životinja. Važno je pomenuti da
je kontinuirano praćenje parazitemije svih tretiranih miševa omogućilo da se zapazi potencijalno
značajan fenomen.
Naime, neka jedinjenja su omogućila da miševi postanu otporni na razvoj cerebralne malarije
i uzrokovala da miševi skloni razvoju neurološkog sindroma tolerišu preživljavanje sa izuzetno velikim
brojem parazita. Poređenjem antimalarijske aktivnosti novosintetisanih aminohinolina uočeno je da
i male strukturne promene u velikoj meri menjaju aktivnost. Rezultati ovog opsežnog istraživanja
su od značaja za buduća istraživanja strukturne modifikacije aminohinolina i doprinose proširenju
znanja u oblasti hemioterapije malarije.
PB  - Belgrade: Serbian Society for Microbiology
C3  - 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
T1  - Experimental treatment of malaria – new perspectives
T1  - Eksperimentalna terapija malarije – novi vidici
EP  - 92
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1453
ER  - 

@conference{
author = "Srbljanović, Jelena and Štajner, Tijana and Bauman, Neda and Lijeskić, Olivera and Opsenica, Igor and Šolaja, Bogdan and Bobić, Branko",
year = "2023",
abstract = "With an estimated 247 million cases annually and 619.000 deaths (in 2021) malaria remains a major
disease of the developing world and globally the most important parasitic disease. Because of
widespread resistance to available antimalarials including chloroquine (CQ) and its derivatives, new
drugs are urgently needed. Here we report on the antimalarial efficacy of new 4-aminoquinoline
derivatives, with modifications at the linker and at the quinoline nucleus.
In vitro screening was performed by the lactate dehydrogenase assay, based on measurement of the
plasmodial lactate dehydrogenase activity in both a CQ-sensitive (3D7) and a CQ-resistant (Dd2)
strain of Plasmodium falciparum, with a CQ as a control. In vivo antimalarial activity was investigated
in C57BL/6 mice infected with Plasmodium berghei ANKA strain by the modified Thompson test.
Compounds were first tested for toxicity. A total of 37 compounds were screened in vitro. Of the 22
that passed the first screening, 18 had IC50 values lower than CQ in the Dd2 strain while only one
was efficient in the 3D7 strain. However, even 15 compounds showed in vivo activity, significantly
(P<0.05) prolonging survival of treated vs. untreated mice. Among these, seven compounds afforded
the survival of 20–100% of treated mice up to Day 31, with or without the detection of parasites
in peripheral blood.
Most importantly, three of these, including ClAQ1, FClAQ1 and ClAQ8, afforded survival of 100% of
animals, the first two at 80 and 160 mg/kg/day and the last only at 160 mg/kg/day.Survival was associated with complete parasite clearance, as shown by both microscopy and qPCR.
Of note, continuous monitoring of parasitemia allowed the observation of a potentially important
phenomenon, that a number of compounds were able to confer resistance to cerebral malaria and
afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.
By comparing the antimalarial activity of this group of novel compounds, we found that even minor
structural modifications substantially affect activity. The results of this extensive study are important,
as they may guide future work involving structural modifications of aminoquinolines, and as a contribution
to the knowledge in the field of malarial chemotherapy., Malarija ostaje globalno najznačajnija parazitska infekcija sa procenjenih 247 miliona slučajeva
i 619.000 smrtnih slučajeva godišnje (2021.). Zbog široko rasprostranjene rezistencije na dostupne
antimalarike, uključujući hlorokvin (CQ) i njegove derivate, hitno su potrebni novi lekovi.
U ovom istraživanju ispitana je potencijalna antimalarijska aktivnost 37 novosintetisanih aminohinolina
sa hemijskim modifikacijama na aminohinolinskom jezgru i bočnom lancu. In vitro skrining aktivnosti
jedinjenja vršen je kolorimetrijskim esejom laktat dehidrogenaze na dva soja Plasmodium falciparum,
osetljivim (3D7) i rezistentnim (Dd2) na CQ, uz CQ kao pozitivnu kontrolu. Aktivnost u in
vivo sistemu je ispitana na ženkama miševa soja C57Bl/6 inficiranim ANKA sojem Plasmodium berghei
primenom modifikovanog Thompson-ovog testa. Ispitivanju aktivnosti jedinjenja prethodila je
faza kliničkog praćenja zdravih životinja terapiranih eksperimentalnim jedinjenjima. Od 37 jedinjenja
ispitanih u fazi in vitro skrininga, 22 koja su inhibirala ≥50% rast bar jednog od dva soja P. falciparum
odabrana su za titraciju do IC50 vrednosti.
Prema soju rezistentnom na CQ, 18 jedinjenja se pokazalo aktivnijim od CQ, dok je među njima samo
jedno jedinjenje bilo aktivnije i prema osetljivom soju. Čak 15 jedinjenja ispitanih u in vivo sistemu
značajno je produžilo život inficiranim životinjama u odnosu na kontrolnu grupu (P < 0.05).
Među njima, sedam jedinjenja je omogućilo preživljavanje 20–100% tretiranih miševa do dana 31, sa
ili bez nalaza parazita u perifernoj krvi. Posebno treba istaći tri jedinjenja koja su dovela do izlečenja
svih tretiranih životinja, ClAQ1 i FClAQ1 (80 i 160 mg/kg/dan) i ClAQ8 (160 mg/kg/dan).Preživljavanje je bilo praćeno i kompletnim klirensom parazita što je dokazano mikroskopskim pregledom
razmaza kao i qPCR analizom krvi i tkiva jetre preživelih životinja. Važno je pomenuti da
je kontinuirano praćenje parazitemije svih tretiranih miševa omogućilo da se zapazi potencijalno
značajan fenomen.
Naime, neka jedinjenja su omogućila da miševi postanu otporni na razvoj cerebralne malarije
i uzrokovala da miševi skloni razvoju neurološkog sindroma tolerišu preživljavanje sa izuzetno velikim
brojem parazita. Poređenjem antimalarijske aktivnosti novosintetisanih aminohinolina uočeno je da
i male strukturne promene u velikoj meri menjaju aktivnost. Rezultati ovog opsežnog istraživanja
su od značaja za buduća istraživanja strukturne modifikacije aminohinolina i doprinose proširenju
znanja u oblasti hemioterapije malarije.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book",
title = "Experimental treatment of malaria – new perspectives, Eksperimentalna terapija malarije – novi vidici",
pages = "92-89",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1453"
}

Srbljanović, J., Štajner, T., Bauman, N., Lijeskić, O., Opsenica, I., Šolaja, B.,& Bobić, B.. (2023). Experimental treatment of malaria – new perspectives. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
Belgrade: Serbian Society for Microbiology., 89-92.
https://hdl.handle.net/21.15107/rcub_rimi_1453

Srbljanović J, Štajner T, Bauman N, Lijeskić O, Opsenica I, Šolaja B, Bobić B. Experimental treatment of malaria – new perspectives. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book. 2023;:89-92.
https://hdl.handle.net/21.15107/rcub_rimi_1453 .

Srbljanović, Jelena, Štajner, Tijana, Bauman, Neda, Lijeskić, Olivera, Opsenica, Igor, Šolaja, Bogdan, Bobić, Branko, "Experimental treatment of malaria – new perspectives" in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book (2023):89-92,
https://hdl.handle.net/21.15107/rcub_rimi_1453 .

TY  - JOUR
AU  - Stelzle, Dominik
AU  - Abraham, Annette
AU  - Kaminski, Miriam
AU  - Schmidt, Veronika
AU  - De Meijere, Robert
AU  - Bustos, Javier A
AU  - Garcia, Hector Hugo
AU  - Sahu, Priyadarshi Soumyaranjan
AU  - Bobić, Branko
AU  - Cretu, Carmen
AU  - Chiodini, Peter
AU  - Dermauw, Veronique
AU  - Devleesschauwer, Brecht
AU  - Dorny, Pierre
AU  - Fonseca, Ana
AU  - Gabriël, Sarah
AU  - Morales, Maria Ángeles Gómez
AU  - Laranjo-González, Minerva
AU  - Hoerauf, Achim
AU  - Hunter, Ewan
AU  - Jambou, Ronan
AU  - Jurhar-Pavlova, Maja
AU  - Reiter-Owona, Ingrid
AU  - Sotiraki, Smaragda
AU  - Trevisan, Chiara
AU  - Vilhena, Manuela
AU  - Walker, Naomi F
AU  - Zammarchi, Lorenzo
AU  - Winkler, Andrea Sylvia
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1320
AB  - Objectives: Neurocysticercosis (NCC) is a parasitic disease caused by the larval stage of the tapeworm Taenia solium. NCC mainly occurs in Africa, Latin America and South-East Asia and can cause a variety of clinical
signs/symptoms. Although it is a rare disease in Europe, it should nonetheless be considered as a differential
diagnosis. The aim of this study was to describe clinical characteristics and management of patients with NCC
diagnosed and treated in Europe.
Methods: We conducted a systematic search of published and unpublished data on patients diagnosed with NCC
in Europe (2000–2019) and extracted demographic, clinical and radiological information on each case, if available. Results: Out of 293 identified NCC cases, 59% of patients presented initially with epileptic seizures (21% focal onset); 52% presented with headache and 54% had other neurological signs/symptoms. The majority of patients had a travel or migration history (76%), mostly from/to Latin America (38%), Africa (32%) or Asia (30%). Treatment varied largely depending on cyst location and number. The outcome was favorable in 90% of the cases.
Conclusions: Management of NCC in Europe varied considerably but often had a good outcome. Travel and
migration to and from areas endemic for T. solium will likely result in continued low prevalence of NCC in Europe.Therefore, training and guidance of clinicians is recommended for optimal patient management.
PB  - Oxford University Press
T2  - Journal of Travel Medicine
T1  - Clinical characteristics and management of neurocysticercosis patients: a retrospective assessment of case reports from Europe
IS  - 1
SP  - taac102
VL  - 30
DO  - 10.1093/jtm/taac102
ER  - 

@article{
author = "Stelzle, Dominik and Abraham, Annette and Kaminski, Miriam and Schmidt, Veronika and De Meijere, Robert and Bustos, Javier A and Garcia, Hector Hugo and Sahu, Priyadarshi Soumyaranjan and Bobić, Branko and Cretu, Carmen and Chiodini, Peter and Dermauw, Veronique and Devleesschauwer, Brecht and Dorny, Pierre and Fonseca, Ana and Gabriël, Sarah and Morales, Maria Ángeles Gómez and Laranjo-González, Minerva and Hoerauf, Achim and Hunter, Ewan and Jambou, Ronan and Jurhar-Pavlova, Maja and Reiter-Owona, Ingrid and Sotiraki, Smaragda and Trevisan, Chiara and Vilhena, Manuela and Walker, Naomi F and Zammarchi, Lorenzo and Winkler, Andrea Sylvia",
year = "2023",
abstract = "Objectives: Neurocysticercosis (NCC) is a parasitic disease caused by the larval stage of the tapeworm Taenia solium. NCC mainly occurs in Africa, Latin America and South-East Asia and can cause a variety of clinical
signs/symptoms. Although it is a rare disease in Europe, it should nonetheless be considered as a differential
diagnosis. The aim of this study was to describe clinical characteristics and management of patients with NCC
diagnosed and treated in Europe.
Methods: We conducted a systematic search of published and unpublished data on patients diagnosed with NCC
in Europe (2000–2019) and extracted demographic, clinical and radiological information on each case, if available. Results: Out of 293 identified NCC cases, 59% of patients presented initially with epileptic seizures (21% focal onset); 52% presented with headache and 54% had other neurological signs/symptoms. The majority of patients had a travel or migration history (76%), mostly from/to Latin America (38%), Africa (32%) or Asia (30%). Treatment varied largely depending on cyst location and number. The outcome was favorable in 90% of the cases.
Conclusions: Management of NCC in Europe varied considerably but often had a good outcome. Travel and
migration to and from areas endemic for T. solium will likely result in continued low prevalence of NCC in Europe.Therefore, training and guidance of clinicians is recommended for optimal patient management.",
publisher = "Oxford University Press",
journal = "Journal of Travel Medicine",
title = "Clinical characteristics and management of neurocysticercosis patients: a retrospective assessment of case reports from Europe",
number = "1",
pages = "taac102",
volume = "30",
doi = "10.1093/jtm/taac102"
}

Stelzle, D., Abraham, A., Kaminski, M., Schmidt, V., De Meijere, R., Bustos, J. A., Garcia, H. H., Sahu, P. S., Bobić, B., Cretu, C., Chiodini, P., Dermauw, V., Devleesschauwer, B., Dorny, P., Fonseca, A., Gabriël, S., Morales, M. Á. G., Laranjo-González, M., Hoerauf, A., Hunter, E., Jambou, R., Jurhar-Pavlova, M., Reiter-Owona, I., Sotiraki, S., Trevisan, C., Vilhena, M., Walker, N. F., Zammarchi, L.,& Winkler, A. S.. (2023). Clinical characteristics and management of neurocysticercosis patients: a retrospective assessment of case reports from Europe. in Journal of Travel Medicine
Oxford University Press., 30(1), taac102.
https://doi.org/10.1093/jtm/taac102

Stelzle D, Abraham A, Kaminski M, Schmidt V, De Meijere R, Bustos JA, Garcia HH, Sahu PS, Bobić B, Cretu C, Chiodini P, Dermauw V, Devleesschauwer B, Dorny P, Fonseca A, Gabriël S, Morales MÁG, Laranjo-González M, Hoerauf A, Hunter E, Jambou R, Jurhar-Pavlova M, Reiter-Owona I, Sotiraki S, Trevisan C, Vilhena M, Walker NF, Zammarchi L, Winkler AS. Clinical characteristics and management of neurocysticercosis patients: a retrospective assessment of case reports from Europe. in Journal of Travel Medicine. 2023;30(1):taac102.
doi:10.1093/jtm/taac102 .

Stelzle, Dominik, Abraham, Annette, Kaminski, Miriam, Schmidt, Veronika, De Meijere, Robert, Bustos, Javier A, Garcia, Hector Hugo, Sahu, Priyadarshi Soumyaranjan, Bobić, Branko, Cretu, Carmen, Chiodini, Peter, Dermauw, Veronique, Devleesschauwer, Brecht, Dorny, Pierre, Fonseca, Ana, Gabriël, Sarah, Morales, Maria Ángeles Gómez, Laranjo-González, Minerva, Hoerauf, Achim, Hunter, Ewan, Jambou, Ronan, Jurhar-Pavlova, Maja, Reiter-Owona, Ingrid, Sotiraki, Smaragda, Trevisan, Chiara, Vilhena, Manuela, Walker, Naomi F, Zammarchi, Lorenzo, Winkler, Andrea Sylvia, "Clinical characteristics and management of neurocysticercosis patients: a retrospective assessment of case reports from Europe" in Journal of Travel Medicine, 30, no. 1 (2023):taac102,
https://doi.org/10.1093/jtm/taac102 . .

TY  - JOUR
AU  - Casulli, Adriano
AU  - Abela-Ridder, Bernadette
AU  - Petrone, Daniele
AU  - Fabiani, Massimo
AU  - Bobić, Branko
AU  - Carmena, David
AU  - Šoba, Barbara
AU  - Zerem, Enver
AU  - Gargaté, Maria João
AU  - Kuzmanovska, Gordana
AU  - Calomfirescu, Cristian
AU  - Rainova, Iskra
AU  - Sotiraki, Smaragda
AU  - Lungu, Vera
AU  - Dezsényi, Balázs
AU  - Herrador, Zaida
AU  - Karamon, Jacek
AU  - Maksimov, Pavlo
AU  - Oksanen, Antti
AU  - Millon, Laurence
AU  - Sviben, Mario
AU  - Shkjezi, Renata
AU  - Gjoni, Valbona
AU  - Akshija, Ilir
AU  - Saarma, Urmas
AU  - Torgerson, Paul R.
AU  - Šnábel, Viliam
AU  - Antolová, Daniela
AU  - Muhović, Damir
AU  - Besim, Hasan
AU  - Chereau, Fanny
AU  - Belhassen García, Moncef
AU  - Chappuis, François
AU  - Gloor, Severin
AU  - Stoeckle, Marcel
AU  - Müllhaupt, Beat
AU  - Manno, Valerio
AU  - Santoro, Azzurra
AU  - Santolamazza, Federica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1323
AB  - The neglected zoonosis cystic echinococcosis affects mainly pastoral and rural communities in both low-income and upper-middle-income countries. In Europe, it should be regarded as an orphan and rare disease. Although human cystic echinococcosis is a notifiable parasitic infectious disease in most European countries, in practice it is largely under-reported by national health systems. To fill this gap, we extracted data on the number, incidence, and trend of human cases in Europe through a systematic review approach, using both the scientific and grey literature and accounting for the period of publication from 1997 to 2021. The highest number of possible human cases at the national level was calculated from various data sources to generate a descriptive model of human cystic echinococcosis in Europe. We identified 64 745 human cystic echinococcosis cases from 40 European countries. The mean annual incidence from 1997 to 2020 throughout Europe was 0·64 cases per 100 000 people and in EU member states was 0·50 cases per 100 000 people. Based on incidence rates and trends detected in this study, the current epicentre of cystic echinococcosis in Europe is in the southeastern European countries, whereas historical endemic European Mediterranean countries have recorded a decrease in the number of cases over the time.
PB  - Elsevier
T2  - The Lancet Infectious Diseases
T1  - Unveiling the incidences and trends of the neglected zoonosis cystic echinococcosis in Europe: a systematic review from the MEmE project
EP  - e107
IS  - 3
SP  - e95
VL  - 23
DO  - 10.1016/S1473-3099(22)00638-7
ER  - 

@article{
author = "Casulli, Adriano and Abela-Ridder, Bernadette and Petrone, Daniele and Fabiani, Massimo and Bobić, Branko and Carmena, David and Šoba, Barbara and Zerem, Enver and Gargaté, Maria João and Kuzmanovska, Gordana and Calomfirescu, Cristian and Rainova, Iskra and Sotiraki, Smaragda and Lungu, Vera and Dezsényi, Balázs and Herrador, Zaida and Karamon, Jacek and Maksimov, Pavlo and Oksanen, Antti and Millon, Laurence and Sviben, Mario and Shkjezi, Renata and Gjoni, Valbona and Akshija, Ilir and Saarma, Urmas and Torgerson, Paul R. and Šnábel, Viliam and Antolová, Daniela and Muhović, Damir and Besim, Hasan and Chereau, Fanny and Belhassen García, Moncef and Chappuis, François and Gloor, Severin and Stoeckle, Marcel and Müllhaupt, Beat and Manno, Valerio and Santoro, Azzurra and Santolamazza, Federica",
year = "2023",
abstract = "The neglected zoonosis cystic echinococcosis affects mainly pastoral and rural communities in both low-income and upper-middle-income countries. In Europe, it should be regarded as an orphan and rare disease. Although human cystic echinococcosis is a notifiable parasitic infectious disease in most European countries, in practice it is largely under-reported by national health systems. To fill this gap, we extracted data on the number, incidence, and trend of human cases in Europe through a systematic review approach, using both the scientific and grey literature and accounting for the period of publication from 1997 to 2021. The highest number of possible human cases at the national level was calculated from various data sources to generate a descriptive model of human cystic echinococcosis in Europe. We identified 64 745 human cystic echinococcosis cases from 40 European countries. The mean annual incidence from 1997 to 2020 throughout Europe was 0·64 cases per 100 000 people and in EU member states was 0·50 cases per 100 000 people. Based on incidence rates and trends detected in this study, the current epicentre of cystic echinococcosis in Europe is in the southeastern European countries, whereas historical endemic European Mediterranean countries have recorded a decrease in the number of cases over the time.",
publisher = "Elsevier",
journal = "The Lancet Infectious Diseases",
title = "Unveiling the incidences and trends of the neglected zoonosis cystic echinococcosis in Europe: a systematic review from the MEmE project",
pages = "e107-e95",
number = "3",
volume = "23",
doi = "10.1016/S1473-3099(22)00638-7"
}

Casulli, A., Abela-Ridder, B., Petrone, D., Fabiani, M., Bobić, B., Carmena, D., Šoba, B., Zerem, E., Gargaté, M. J., Kuzmanovska, G., Calomfirescu, C., Rainova, I., Sotiraki, S., Lungu, V., Dezsényi, B., Herrador, Z., Karamon, J., Maksimov, P., Oksanen, A., Millon, L., Sviben, M., Shkjezi, R., Gjoni, V., Akshija, I., Saarma, U., Torgerson, P. R., Šnábel, V., Antolová, D., Muhović, D., Besim, H., Chereau, F., Belhassen García, M., Chappuis, F., Gloor, S., Stoeckle, M., Müllhaupt, B., Manno, V., Santoro, A.,& Santolamazza, F.. (2023). Unveiling the incidences and trends of the neglected zoonosis cystic echinococcosis in Europe: a systematic review from the MEmE project. in The Lancet Infectious Diseases
Elsevier., 23(3), e95-e107.
https://doi.org/10.1016/S1473-3099(22)00638-7

Casulli A, Abela-Ridder B, Petrone D, Fabiani M, Bobić B, Carmena D, Šoba B, Zerem E, Gargaté MJ, Kuzmanovska G, Calomfirescu C, Rainova I, Sotiraki S, Lungu V, Dezsényi B, Herrador Z, Karamon J, Maksimov P, Oksanen A, Millon L, Sviben M, Shkjezi R, Gjoni V, Akshija I, Saarma U, Torgerson PR, Šnábel V, Antolová D, Muhović D, Besim H, Chereau F, Belhassen García M, Chappuis F, Gloor S, Stoeckle M, Müllhaupt B, Manno V, Santoro A, Santolamazza F. Unveiling the incidences and trends of the neglected zoonosis cystic echinococcosis in Europe: a systematic review from the MEmE project. in The Lancet Infectious Diseases. 2023;23(3):e95-e107.
doi:10.1016/S1473-3099(22)00638-7 .

Casulli, Adriano, Abela-Ridder, Bernadette, Petrone, Daniele, Fabiani, Massimo, Bobić, Branko, Carmena, David, Šoba, Barbara, Zerem, Enver, Gargaté, Maria João, Kuzmanovska, Gordana, Calomfirescu, Cristian, Rainova, Iskra, Sotiraki, Smaragda, Lungu, Vera, Dezsényi, Balázs, Herrador, Zaida, Karamon, Jacek, Maksimov, Pavlo, Oksanen, Antti, Millon, Laurence, Sviben, Mario, Shkjezi, Renata, Gjoni, Valbona, Akshija, Ilir, Saarma, Urmas, Torgerson, Paul R., Šnábel, Viliam, Antolová, Daniela, Muhović, Damir, Besim, Hasan, Chereau, Fanny, Belhassen García, Moncef, Chappuis, François, Gloor, Severin, Stoeckle, Marcel, Müllhaupt, Beat, Manno, Valerio, Santoro, Azzurra, Santolamazza, Federica, "Unveiling the incidences and trends of the neglected zoonosis cystic echinococcosis in Europe: a systematic review from the MEmE project" in The Lancet Infectious Diseases, 23, no. 3 (2023):e95-e107,
https://doi.org/10.1016/S1473-3099(22)00638-7 . .

TY  - JOUR
AU  - Stopić, Milena
AU  - Štajner, Tijana
AU  - Marković-Denić, Ljiljana
AU  - Nikolić, Vladimir
AU  - Đilas, Iva
AU  - Jovanović Srzentić, Snežana
AU  - Đurković-Đaković, Olgica
AU  - Bobić, Branko
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1224
AB  - Toxoplasmosis is a globally distributed parasitic zoonosis, affecting approximately one third of the human population. Epidemiological studies on toxoplasmosis conducted in Serbia so far have been focused on women of childbearing age, without a clear insight into the prevalence in the general population. We conducted a cross-sectional study in a representative sample of the healthy adult population consisting of 1095 blood donors of both genders to establish the prevalence and risk factors for Toxoplasma gondii infection. Data on the demographic and clinical characteristics of all study participants, as well as on their lifestyle habits, were collected by means of a questionnaire. The overall prevalence of infection was 20.5% (224/1095) and the avidity of the specific IgG antibodies detected was high in a vast majority of the seropositive donors (98.2%). Interestingly, the remaining 1.8% of the specific IgG positive samples were of borderline avidity (4/224), in complete absence of specific IgM. The multivariate logistic regression analysis showed that independent risk factors included age (from OR (95% CI) 1.9 (1.13–3.28) in the 30–39 age group, to 6.8 (3.27–14.24) in the age group of >60 years), suburban living (OR (95% CI) 2.2 (1.43–3.34)) and contact with soil (OR (95% CI) 1.4 (1.01–1.94)). This first large-scale study on toxoplasmosis in the general population in Serbia shows the lowest prevalence ever reported in this country. Moreover, the novel perspective on risk factors provides an updated basis for future prevention programs.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Microorganisms
T1  - Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors
IS  - 3
SP  - 492
VL  - 10
DO  - 10.3390/microorganisms10030492
ER  - 

@article{
author = "Stopić, Milena and Štajner, Tijana and Marković-Denić, Ljiljana and Nikolić, Vladimir and Đilas, Iva and Jovanović Srzentić, Snežana and Đurković-Đaković, Olgica and Bobić, Branko",
year = "2022",
abstract = "Toxoplasmosis is a globally distributed parasitic zoonosis, affecting approximately one third of the human population. Epidemiological studies on toxoplasmosis conducted in Serbia so far have been focused on women of childbearing age, without a clear insight into the prevalence in the general population. We conducted a cross-sectional study in a representative sample of the healthy adult population consisting of 1095 blood donors of both genders to establish the prevalence and risk factors for Toxoplasma gondii infection. Data on the demographic and clinical characteristics of all study participants, as well as on their lifestyle habits, were collected by means of a questionnaire. The overall prevalence of infection was 20.5% (224/1095) and the avidity of the specific IgG antibodies detected was high in a vast majority of the seropositive donors (98.2%). Interestingly, the remaining 1.8% of the specific IgG positive samples were of borderline avidity (4/224), in complete absence of specific IgM. The multivariate logistic regression analysis showed that independent risk factors included age (from OR (95% CI) 1.9 (1.13–3.28) in the 30–39 age group, to 6.8 (3.27–14.24) in the age group of >60 years), suburban living (OR (95% CI) 2.2 (1.43–3.34)) and contact with soil (OR (95% CI) 1.4 (1.01–1.94)). This first large-scale study on toxoplasmosis in the general population in Serbia shows the lowest prevalence ever reported in this country. Moreover, the novel perspective on risk factors provides an updated basis for future prevention programs.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Microorganisms",
title = "Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors",
number = "3",
pages = "492",
volume = "10",
doi = "10.3390/microorganisms10030492"
}

Stopić, M., Štajner, T., Marković-Denić, L., Nikolić, V., Đilas, I., Jovanović Srzentić, S., Đurković-Đaković, O.,& Bobić, B.. (2022). Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors. in Microorganisms
Multidisciplinary Digital Publishing Institute (MDPI)., 10(3), 492.
https://doi.org/10.3390/microorganisms10030492

Stopić M, Štajner T, Marković-Denić L, Nikolić V, Đilas I, Jovanović Srzentić S, Đurković-Đaković O, Bobić B. Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors. in Microorganisms. 2022;10(3):492.
doi:10.3390/microorganisms10030492 .

Stopić, Milena, Štajner, Tijana, Marković-Denić, Ljiljana, Nikolić, Vladimir, Đilas, Iva, Jovanović Srzentić, Snežana, Đurković-Đaković, Olgica, Bobić, Branko, "Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors" in Microorganisms, 10, no. 3 (2022):492,
https://doi.org/10.3390/microorganisms10030492 . .

TY  - JOUR
AU  - Betić, Nikola
AU  - Karabasil, Neđeljko
AU  - Đurković-Đaković, Olgica
AU  - Ćirković, Vladimir
AU  - Bobić, Branko
AU  - Branković Lazić, Ivana
AU  - Đorđević, Vesna
AU  - Klun, Ivana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1240
AB  - Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.
T2  - Microorganisms
T1  - Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures
IS  - 5
SP  - 1069
VL  - 10
DO  - 10.3390/microorganisms10051069
ER  - 

@article{
author = "Betić, Nikola and Karabasil, Neđeljko and Đurković-Đaković, Olgica and Ćirković, Vladimir and Bobić, Branko and Branković Lazić, Ivana and Đorđević, Vesna and Klun, Ivana",
year = "2022",
abstract = "Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.",
journal = "Microorganisms",
title = "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures",
number = "5",
pages = "1069",
volume = "10",
doi = "10.3390/microorganisms10051069"
}

Betić, N., Karabasil, N., Đurković-Đaković, O., Ćirković, V., Bobić, B., Branković Lazić, I., Đorđević, V.,& Klun, I.. (2022). Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms, 10(5), 1069.
https://doi.org/10.3390/microorganisms10051069

Betić N, Karabasil N, Đurković-Đaković O, Ćirković V, Bobić B, Branković Lazić I, Đorđević V, Klun I. Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms. 2022;10(5):1069.
doi:10.3390/microorganisms10051069 .

Betić, Nikola, Karabasil, Neđeljko, Đurković-Đaković, Olgica, Ćirković, Vladimir, Bobić, Branko, Branković Lazić, Ivana, Đorđević, Vesna, Klun, Ivana, "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures" in Microorganisms, 10, no. 5 (2022):1069,
https://doi.org/10.3390/microorganisms10051069 . .

TY  - JOUR
AU  - Guegan, Helene
AU  - Štajner, Tijana
AU  - Bobić, Branko
AU  - Press, Cindy
AU  - Olariu, Rares T.
AU  - Olson, Kjerstie
AU  - Srbljanović, Jelena
AU  - Montoya, Jose G.
AU  - Đurković-Đaković, Olgica
AU  - Robert-Gangneux, Florence
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1129
AB  - Neonatal diagnosis of congenital toxoplasmosis is based on a combination of serological and molecular tests. Maternal screening and treatment differ according to national policies and may impact the sensitivity of diagnostic methods in infants at birth. In this multicenter study, 115 neonates born to 61 treated (53%) and 54 (47%) untreated women were retrospectively included in three centers (France, Serbia, and the United States) to assess the impact of maternal anti-Toxoplasma treatment on the performance of neonatal workup at birth (neosynthesized anti-Toxoplasma IgM, IgA, and IgG and quantitative PCR [qPCR]) using univariate and multivariate approaches. Independently of the time of maternal seroconversion, the serological techniques were impacted differently by maternal treatment. The detection of IgM by immunosorbent agglutination assay (ISAGA) and Western blotting (WB) dropped from 90.7% and 88.2% in untreated neonates to 533% and 51.9% in treated neonates (P  lt  0.05), whereas IgM enzyme-linked immunosorbent assay (ELISA) and IgA ISAGA were not significantly affected by maternal treatment. A 2-fold reduction in the sensitivity of neosynthesized IgG by WB was also observed in the case of treatment during pregnancy (37.7% versus 82.3%). Interestingly, the effect of treatment was shown to be duration dependent, especially for IgM detection, when the treatment course exceeded 8 weeks, whatever the therapy. The sensitivity of Toxoplasma PCR in blood was also lowered by maternal treatment from 39.1% to 23.2%. These results highlight that anti-Toxoplasma therapy during pregnancy may set back biological evidence of neonatal infection at birth and underline the need for a careful serological follow-up of infants with normal workup.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Clinical Microbiology
T1  - Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate
IS  - 2
VL  - 59
DO  - 10.1128/JCM.01368-20
ER  - 

@article{
author = "Guegan, Helene and Štajner, Tijana and Bobić, Branko and Press, Cindy and Olariu, Rares T. and Olson, Kjerstie and Srbljanović, Jelena and Montoya, Jose G. and Đurković-Đaković, Olgica and Robert-Gangneux, Florence",
year = "2021",
abstract = "Neonatal diagnosis of congenital toxoplasmosis is based on a combination of serological and molecular tests. Maternal screening and treatment differ according to national policies and may impact the sensitivity of diagnostic methods in infants at birth. In this multicenter study, 115 neonates born to 61 treated (53%) and 54 (47%) untreated women were retrospectively included in three centers (France, Serbia, and the United States) to assess the impact of maternal anti-Toxoplasma treatment on the performance of neonatal workup at birth (neosynthesized anti-Toxoplasma IgM, IgA, and IgG and quantitative PCR [qPCR]) using univariate and multivariate approaches. Independently of the time of maternal seroconversion, the serological techniques were impacted differently by maternal treatment. The detection of IgM by immunosorbent agglutination assay (ISAGA) and Western blotting (WB) dropped from 90.7% and 88.2% in untreated neonates to 533% and 51.9% in treated neonates (P  lt  0.05), whereas IgM enzyme-linked immunosorbent assay (ELISA) and IgA ISAGA were not significantly affected by maternal treatment. A 2-fold reduction in the sensitivity of neosynthesized IgG by WB was also observed in the case of treatment during pregnancy (37.7% versus 82.3%). Interestingly, the effect of treatment was shown to be duration dependent, especially for IgM detection, when the treatment course exceeded 8 weeks, whatever the therapy. The sensitivity of Toxoplasma PCR in blood was also lowered by maternal treatment from 39.1% to 23.2%. These results highlight that anti-Toxoplasma therapy during pregnancy may set back biological evidence of neonatal infection at birth and underline the need for a careful serological follow-up of infants with normal workup.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Clinical Microbiology",
title = "Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate",
number = "2",
volume = "59",
doi = "10.1128/JCM.01368-20"
}

Guegan, H., Štajner, T., Bobić, B., Press, C., Olariu, R. T., Olson, K., Srbljanović, J., Montoya, J. G., Đurković-Đaković, O.,& Robert-Gangneux, F.. (2021). Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate. in Journal of Clinical Microbiology
Amer Soc Microbiology, Washington., 59(2).
https://doi.org/10.1128/JCM.01368-20

Guegan H, Štajner T, Bobić B, Press C, Olariu RT, Olson K, Srbljanović J, Montoya JG, Đurković-Đaković O, Robert-Gangneux F. Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate. in Journal of Clinical Microbiology. 2021;59(2).
doi:10.1128/JCM.01368-20 .

Guegan, Helene, Štajner, Tijana, Bobić, Branko, Press, Cindy, Olariu, Rares T., Olson, Kjerstie, Srbljanović, Jelena, Montoya, Jose G., Đurković-Đaković, Olgica, Robert-Gangneux, Florence, "Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate" in Journal of Clinical Microbiology, 59, no. 2 (2021),
https://doi.org/10.1128/JCM.01368-20 . .

TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Ćirković, Vladimir
AU  - Bauman, Neda
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Lijeskić, Olivera
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1190
AB  - In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.
PB  - MDPI
T2  - Microorganisms
T1  - Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange
IS  - 12
SP  - 2526
VL  - 9
DO  - 10.3390/microorganisms9122526
ER  - 

@article{
author = "Uzelac, Aleksandra and Klun, Ivana and Ćirković, Vladimir and Bauman, Neda and Bobić, Branko and Štajner, Tijana and Srbljanović, Jelena and Lijeskić, Olivera and Đurković-Đaković, Olgica",
year = "2021",
abstract = "In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange",
number = "12",
pages = "2526",
volume = "9",
doi = "10.3390/microorganisms9122526"
}

Uzelac, A., Klun, I., Ćirković, V., Bauman, N., Bobić, B., Štajner, T., Srbljanović, J., Lijeskić, O.,& Đurković-Đaković, O.. (2021). Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms
MDPI., 9(12), 2526.
https://doi.org/10.3390/microorganisms9122526

Uzelac A, Klun I, Ćirković V, Bauman N, Bobić B, Štajner T, Srbljanović J, Lijeskić O, Đurković-Đaković O. Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms. 2021;9(12):2526.
doi:10.3390/microorganisms9122526 .

Uzelac, Aleksandra, Klun, Ivana, Ćirković, Vladimir, Bauman, Neda, Bobić, Branko, Štajner, Tijana, Srbljanović, Jelena, Lijeskić, Olivera, Đurković-Đaković, Olgica, "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange" in Microorganisms, 9, no. 12 (2021):2526,
https://doi.org/10.3390/microorganisms9122526 . .

TY  - JOUR
AU  - Lijeskić, Olivera
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Radosavljević, Aleksandra
AU  - Bobić, Branko
AU  - Klun, Ivana
AU  - Stanojević-Paović, Anka
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1198
AB  - Introduction: Ocular toxoplasmosis is the most common cause of infectious posterior uveitis worldwide. It can be prenatal or postnatal in origin. Despite estimations that postnatal ocular toxoplasmosis is more prevalent, only several cases of proven postnatal ocular toxoplasmosis have been reported in non-epidemic settings. Here, the clinical evolution of ocular toxoplasmosis of conclusively proven postnatal origin in immunocompetent patients is reported.Methodology: Postnatal ocular toxoplasmosis was diagnosed based on clinical diagnosis supported by the longitudinal detection of Toxoplasma gondii-specific IgG, IgM and IgA antibodies in the serum as well as by direct detection of the parasite (bioassay) and/or its DNA (real-time PCR) in aqueous humor.Results: Three cases involved adults in whom ocular toxoplasmosis developed during primary T. gondii infection, as part of the clinical presentation in two and as the sole manifestation in one patient. The fourth patient was a case of inactive ocular toxoplasmosis in a 14-year-old boy, where postnatal infection was confirmed by exclusion of maternal infection. The causative parasite strain was genotyped in only one case and it belonged to genotype II, the dominant type in Europe. One patient acquired the infection in Africa, suggesting an atypical strain.Conclusions: The distinction between prenatal and postnatal ocular toxoplasmosis is only possible in particular clinical situations, and requires extensive laboratory investigation. Genotyping of the parasite strain involved may be important, particularly if atypical strains are suspected, requiring tailored treatment approaches.
T2  - The Journal of Infection in Developing Countries
T1  - Postnatal ocular toxoplasmosis in immunocompetent patients
EP  - 1522
IS  - 10
SP  - 1515
VL  - 15
DO  - 10.3855/jidc.14824
ER  - 

@article{
author = "Lijeskić, Olivera and Štajner, Tijana and Srbljanović, Jelena and Radosavljević, Aleksandra and Bobić, Branko and Klun, Ivana and Stanojević-Paović, Anka and Đurković-Đaković, Olgica",
year = "2021",
abstract = "Introduction: Ocular toxoplasmosis is the most common cause of infectious posterior uveitis worldwide. It can be prenatal or postnatal in origin. Despite estimations that postnatal ocular toxoplasmosis is more prevalent, only several cases of proven postnatal ocular toxoplasmosis have been reported in non-epidemic settings. Here, the clinical evolution of ocular toxoplasmosis of conclusively proven postnatal origin in immunocompetent patients is reported.Methodology: Postnatal ocular toxoplasmosis was diagnosed based on clinical diagnosis supported by the longitudinal detection of Toxoplasma gondii-specific IgG, IgM and IgA antibodies in the serum as well as by direct detection of the parasite (bioassay) and/or its DNA (real-time PCR) in aqueous humor.Results: Three cases involved adults in whom ocular toxoplasmosis developed during primary T. gondii infection, as part of the clinical presentation in two and as the sole manifestation in one patient. The fourth patient was a case of inactive ocular toxoplasmosis in a 14-year-old boy, where postnatal infection was confirmed by exclusion of maternal infection. The causative parasite strain was genotyped in only one case and it belonged to genotype II, the dominant type in Europe. One patient acquired the infection in Africa, suggesting an atypical strain.Conclusions: The distinction between prenatal and postnatal ocular toxoplasmosis is only possible in particular clinical situations, and requires extensive laboratory investigation. Genotyping of the parasite strain involved may be important, particularly if atypical strains are suspected, requiring tailored treatment approaches.",
journal = "The Journal of Infection in Developing Countries",
title = "Postnatal ocular toxoplasmosis in immunocompetent patients",
pages = "1522-1515",
number = "10",
volume = "15",
doi = "10.3855/jidc.14824"
}

Lijeskić, O., Štajner, T., Srbljanović, J., Radosavljević, A., Bobić, B., Klun, I., Stanojević-Paović, A.,& Đurković-Đaković, O.. (2021). Postnatal ocular toxoplasmosis in immunocompetent patients. in The Journal of Infection in Developing Countries, 15(10), 1515-1522.
https://doi.org/10.3855/jidc.14824

Lijeskić O, Štajner T, Srbljanović J, Radosavljević A, Bobić B, Klun I, Stanojević-Paović A, Đurković-Đaković O. Postnatal ocular toxoplasmosis in immunocompetent patients. in The Journal of Infection in Developing Countries. 2021;15(10):1515-1522.
doi:10.3855/jidc.14824 .

Lijeskić, Olivera, Štajner, Tijana, Srbljanović, Jelena, Radosavljević, Aleksandra, Bobić, Branko, Klun, Ivana, Stanojević-Paović, Anka, Đurković-Đaković, Olgica, "Postnatal ocular toxoplasmosis in immunocompetent patients" in The Journal of Infection in Developing Countries, 15, no. 10 (2021):1515-1522,
https://doi.org/10.3855/jidc.14824 . .

TY  - JOUR
AU  - Bobić, Branko
AU  - Ćirković, Vladimir
AU  - Klun, Ivana
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Bauman, Neda
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1267
AB  - Taenia solium is a zoonotic parasite that causes taeniasis and cysticercosis in humans (as final hosts) and cysticercosis in pigs (as intermediate hosts). The Russian Federation (RF) is traditionally considered as endemic for this zoonosis. However, the epidemiological data on T. solium infection have not been reviewed for the past 20 years, in which time dynamic economical and societal changes have occurred in the RF. The aim of this systematic review was to analyse the status of T. solium infection in RF in the 2000–2019 period. A literature search was conducted, which collected published articles, grey literature and official data on the epidemiology of T. solium taeniasis and cysticercosis in the RF published from 2000. From a total of 2021 articles and 24 official reports originally returned by the search, data were extracted from 12 full text articles and 11 official reports. Taenia solium taeniasis was continuously reported in the RF between 2000 and 2019, with a tenfold decrease in the incidence, from 0.2 per 100,000 population in 2000 to 0.023/100,000 in 2019. Also, the number of administrative units where taeniasis was detected continuously decreased. Cysticercosis in pigs had a declining trend after 2006. In conclusion, although decreasing, T. solium infection is still endemic in several regions and suspected to be endemic in most of the RF.
PB  - Cambridge University Press
T2  - Journal of Helminthology
T1  - Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review
SP  - e49
VL  - 95
DO  - 10.1017/S0022149X21000432
ER  - 

@article{
author = "Bobić, Branko and Ćirković, Vladimir and Klun, Ivana and Štajner, Tijana and Srbljanović, Jelena and Bauman, Neda and Đurković-Đaković, Olgica",
year = "2021",
abstract = "Taenia solium is a zoonotic parasite that causes taeniasis and cysticercosis in humans (as final hosts) and cysticercosis in pigs (as intermediate hosts). The Russian Federation (RF) is traditionally considered as endemic for this zoonosis. However, the epidemiological data on T. solium infection have not been reviewed for the past 20 years, in which time dynamic economical and societal changes have occurred in the RF. The aim of this systematic review was to analyse the status of T. solium infection in RF in the 2000–2019 period. A literature search was conducted, which collected published articles, grey literature and official data on the epidemiology of T. solium taeniasis and cysticercosis in the RF published from 2000. From a total of 2021 articles and 24 official reports originally returned by the search, data were extracted from 12 full text articles and 11 official reports. Taenia solium taeniasis was continuously reported in the RF between 2000 and 2019, with a tenfold decrease in the incidence, from 0.2 per 100,000 population in 2000 to 0.023/100,000 in 2019. Also, the number of administrative units where taeniasis was detected continuously decreased. Cysticercosis in pigs had a declining trend after 2006. In conclusion, although decreasing, T. solium infection is still endemic in several regions and suspected to be endemic in most of the RF.",
publisher = "Cambridge University Press",
journal = "Journal of Helminthology",
title = "Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review",
pages = "e49",
volume = "95",
doi = "10.1017/S0022149X21000432"
}

Bobić, B., Ćirković, V., Klun, I., Štajner, T., Srbljanović, J., Bauman, N.,& Đurković-Đaković, O.. (2021). Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review. in Journal of Helminthology
Cambridge University Press., 95, e49.
https://doi.org/10.1017/S0022149X21000432

Bobić B, Ćirković V, Klun I, Štajner T, Srbljanović J, Bauman N, Đurković-Đaković O. Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review. in Journal of Helminthology. 2021;95:e49.
doi:10.1017/S0022149X21000432 .

Bobić, Branko, Ćirković, Vladimir, Klun, Ivana, Štajner, Tijana, Srbljanović, Jelena, Bauman, Neda, Đurković-Đaković, Olgica, "Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review" in Journal of Helminthology, 95 (2021):e49,
https://doi.org/10.1017/S0022149X21000432 . .

TY  - JOUR
AU  - Srbljanović, Jelena
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Uzelac, Aleksandra
AU  - Opsenica, Igor
AU  - Terzić-Jovanović, Nataša
AU  - Bauman, Neda
AU  - Šolaja, Bogdan
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/998
AB  - Objectives: Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. Methods: In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falciparum strain 3D7 and CQ-resistant (CQ(R)) P. falciparum strain Dd2. In vivo activity was evaluated by a modified Thompson test using C57BL/6 mice infected with Plasmodium berghei ANKA strain. Results: Nine of the ten compounds had a lower 50% inhibitory concentration (IC50) than CQ against the CQ(R) strain Dd2. Five of these compounds that were available for in vivo evaluation were shown to be nontoxic. All five compounds administered at a dose of 160 mg/kg/day for 3 days prolonged the survival of treated compared with untreated mice. Untreated control mice died by Day 7 with a mean parasitaemia of 15%. Among treated mice, a dichotomous outcome was observed, with a two-third majority of treated mice dying by Day 17 with a low mean parasitaemia of 5%, whilst one-third survived longer with a mean hyperparasitaemia of 70%; specifically, five of these mice survived a mean of 25 days, whilst two even survived past Day 31. Conclusions: The significant antimalarial potential of this aminoquinoline series is illustrated by its excellent in vitro activity against the CQ(R) P. falciparum strain and significant in vivo activity. Interestingly, compounds CIAQ7, CIAQ9 and CIAQ11 were able to confer resistance to cerebral malaria and afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Global Antimicrobial Resistance
T1  - Aminoquinolines afford resistance to cerebral malaria in susceptible mice
EP  - 25
SP  - 20
VL  - 23
DO  - 10.1016/j.jgar.2020.07.027
ER  - 

@article{
author = "Srbljanović, Jelena and Bobić, Branko and Štajner, Tijana and Uzelac, Aleksandra and Opsenica, Igor and Terzić-Jovanović, Nataša and Bauman, Neda and Šolaja, Bogdan and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Objectives: Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. Methods: In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falciparum strain 3D7 and CQ-resistant (CQ(R)) P. falciparum strain Dd2. In vivo activity was evaluated by a modified Thompson test using C57BL/6 mice infected with Plasmodium berghei ANKA strain. Results: Nine of the ten compounds had a lower 50% inhibitory concentration (IC50) than CQ against the CQ(R) strain Dd2. Five of these compounds that were available for in vivo evaluation were shown to be nontoxic. All five compounds administered at a dose of 160 mg/kg/day for 3 days prolonged the survival of treated compared with untreated mice. Untreated control mice died by Day 7 with a mean parasitaemia of 15%. Among treated mice, a dichotomous outcome was observed, with a two-third majority of treated mice dying by Day 17 with a low mean parasitaemia of 5%, whilst one-third survived longer with a mean hyperparasitaemia of 70%; specifically, five of these mice survived a mean of 25 days, whilst two even survived past Day 31. Conclusions: The significant antimalarial potential of this aminoquinoline series is illustrated by its excellent in vitro activity against the CQ(R) P. falciparum strain and significant in vivo activity. Interestingly, compounds CIAQ7, CIAQ9 and CIAQ11 were able to confer resistance to cerebral malaria and afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Global Antimicrobial Resistance",
title = "Aminoquinolines afford resistance to cerebral malaria in susceptible mice",
pages = "25-20",
volume = "23",
doi = "10.1016/j.jgar.2020.07.027"
}

Srbljanović, J., Bobić, B., Štajner, T., Uzelac, A., Opsenica, I., Terzić-Jovanović, N., Bauman, N., Šolaja, B.,& Đurković-Đaković, O.. (2020). Aminoquinolines afford resistance to cerebral malaria in susceptible mice. in Journal of Global Antimicrobial Resistance
Elsevier Sci Ltd, Oxford., 23, 20-25.
https://doi.org/10.1016/j.jgar.2020.07.027

Srbljanović J, Bobić B, Štajner T, Uzelac A, Opsenica I, Terzić-Jovanović N, Bauman N, Šolaja B, Đurković-Đaković O. Aminoquinolines afford resistance to cerebral malaria in susceptible mice. in Journal of Global Antimicrobial Resistance. 2020;23:20-25.
doi:10.1016/j.jgar.2020.07.027 .

Srbljanović, Jelena, Bobić, Branko, Štajner, Tijana, Uzelac, Aleksandra, Opsenica, Igor, Terzić-Jovanović, Nataša, Bauman, Neda, Šolaja, Bogdan, Đurković-Đaković, Olgica, "Aminoquinolines afford resistance to cerebral malaria in susceptible mice" in Journal of Global Antimicrobial Resistance, 23 (2020):20-25,
https://doi.org/10.1016/j.jgar.2020.07.027 . .

TY  - JOUR
AU  - Bauman, Neda
AU  - Ilić, Anđelija
AU  - Lijeskić, Olivera
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Srbljanović, Jelena
AU  - Ćirković, Vladimir
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1046
AB  - Toxoplasma gondiiis an obligate intracellular parasite infecting up to one third of the human population. The central event in the pathogenesis of toxoplasmosis is the conversion of tachyzoites into encysted bradyzoites. A novel approach to analyze the structure ofin vivo-derived tissue cysts may be the increasingly used computational image analysis. The objective of this study was to quantify the geometrical complexity ofT.gondiicysts by morphological, particle, and fractal analysis, as well as to determine if it is impacted by parasite strain, cyst age, and host type. A total of 31 images ofT.gondiibrain cysts of four type-2 strains (Me49, and local isolates BGD1, BGD14, and BGD26) was analyzed using ImageJ software. The parameters of interest included diameter, circularity, packing density (PD), fractal dimension (FD), and lacunarity. Although cyst diameter varied widely, its negative correlation with PD was observed. Circularity was remarkably close to 1, indicating a perfectly round shape of the cysts. PD and FD did not vary among cysts of different strains, age, and derived from mice of different genetic background. Conversely, lacunarity, which is a measure of heterogeneity, was significantly lower for BGD1 strain vs. all other strains, and higher for Me49 vs. BGD14 and BGD26, but did not differ among Me49 cysts of different age, or those derived from genetically different mice. The results indicate a highly uniform structure and occupancy of the differentT.gondiitissue cysts. This study furthers the use of image analysis in describing the structural complexity ofT.gondiicyst morphology, and presents the first application of fractal analysis for this purpose. The presented results show that use of a freely available software is a cost-effective approach to advance automated image scoring forT.gondiicysts.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts
IS  - 8
SP  - e0234169
VL  - 15
DO  - 10.1371/journal.pone.0234169
ER  - 

@article{
author = "Bauman, Neda and Ilić, Anđelija and Lijeskić, Olivera and Uzelac, Aleksandra and Klun, Ivana and Srbljanović, Jelena and Ćirković, Vladimir and Bobić, Branko and Štajner, Tijana and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Toxoplasma gondiiis an obligate intracellular parasite infecting up to one third of the human population. The central event in the pathogenesis of toxoplasmosis is the conversion of tachyzoites into encysted bradyzoites. A novel approach to analyze the structure ofin vivo-derived tissue cysts may be the increasingly used computational image analysis. The objective of this study was to quantify the geometrical complexity ofT.gondiicysts by morphological, particle, and fractal analysis, as well as to determine if it is impacted by parasite strain, cyst age, and host type. A total of 31 images ofT.gondiibrain cysts of four type-2 strains (Me49, and local isolates BGD1, BGD14, and BGD26) was analyzed using ImageJ software. The parameters of interest included diameter, circularity, packing density (PD), fractal dimension (FD), and lacunarity. Although cyst diameter varied widely, its negative correlation with PD was observed. Circularity was remarkably close to 1, indicating a perfectly round shape of the cysts. PD and FD did not vary among cysts of different strains, age, and derived from mice of different genetic background. Conversely, lacunarity, which is a measure of heterogeneity, was significantly lower for BGD1 strain vs. all other strains, and higher for Me49 vs. BGD14 and BGD26, but did not differ among Me49 cysts of different age, or those derived from genetically different mice. The results indicate a highly uniform structure and occupancy of the differentT.gondiitissue cysts. This study furthers the use of image analysis in describing the structural complexity ofT.gondiicyst morphology, and presents the first application of fractal analysis for this purpose. The presented results show that use of a freely available software is a cost-effective approach to advance automated image scoring forT.gondiicysts.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts",
number = "8",
pages = "e0234169",
volume = "15",
doi = "10.1371/journal.pone.0234169"
}

Bauman, N., Ilić, A., Lijeskić, O., Uzelac, A., Klun, I., Srbljanović, J., Ćirković, V., Bobić, B., Štajner, T.,& Đurković-Đaković, O.. (2020). Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts. in PLoS One
Public Library Science, San Francisco., 15(8), e0234169.
https://doi.org/10.1371/journal.pone.0234169

Bauman N, Ilić A, Lijeskić O, Uzelac A, Klun I, Srbljanović J, Ćirković V, Bobić B, Štajner T, Đurković-Đaković O. Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts. in PLoS One. 2020;15(8):e0234169.
doi:10.1371/journal.pone.0234169 .

Bauman, Neda, Ilić, Anđelija, Lijeskić, Olivera, Uzelac, Aleksandra, Klun, Ivana, Srbljanović, Jelena, Ćirković, Vladimir, Bobić, Branko, Štajner, Tijana, Đurković-Đaković, Olgica, "Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts" in PLoS One, 15, no. 8 (2020):e0234169,
https://doi.org/10.1371/journal.pone.0234169 . .

TY  - JOUR
AU  - Eichenberger, Ramon M.
AU  - Thomas, Lian F.
AU  - Gabriel, Sarah
AU  - Bobić, Branko
AU  - Devleesschauwer, Brecht
AU  - Robertson, Lucy J.
AU  - Saratsis, Anastasios
AU  - Torgerson, Paul R.
AU  - Braae, Uffe C.
AU  - Dermauw, Veronique
AU  - Dorny, Pierre
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1056
AB  - BackgroundTaenia saginata is an important zoonotic parasite, causing taeniosis in humans and cysticercosis in bovines, the latter being a significant concern for the global beef industry. Many countries in East, Southeast and South Asia are experiencing rapid economic growth, and an increasing number of people in these countries are dependent on the livestock industry. Currently, however, an overview of the prevalence of T. saginata in this region is lacking. In this review, we analysed the available literature on T. saginata taeniosis and bovine cysticercosis for East, Southeast and South Asia.MethodsA systematic review was conducted, based on both published and grey literature. Articles published between 1990 and 2017 were mined for information on the occurrence, prevalence, and geographical distribution of T. saginata taeniosis and bovine cysticercosis in East, Southeast and South Asia.ResultsThe presence of T. saginata was described in 15 of 27 countries of the region, including Afghanistan, Cambodia, China, India, Indonesia, Japan, Lao PDR, Malaysia, Mongolia, Nepal, Pakistan, Philippines, South Korea, Thailand and Vietnam. The only country that reported an absence of T. saginata is Japan, although sporadic reports of imported cases and unconfirmed reports of autochthonous infections were identified. Nationwide surveys of taeniosis with systematic sample collection and high sample numbers were available for Cambodia, China, Lao PDR, and South Korea, although speciation of Taenia was not always performed. Regional prevalence of taeniosis and bovine cysticercosis in endemic regions ranged between 0.02-42.6%, and 0.76-46.7%, respectively. However, data for bovine cysticercosis were only available for five countries (Japan, Lao PDR, Mongolia, Pakistan and Vietnam).ConclusionsThe data indicate a widespread occurrence of T. saginata throughout East, Southeast and South Asia. Identification of Taenia spp. in human infections was frequently not performed, leading to gaps in knowledge about the distribution of human tapeworm infections, mainly in regions where different human Taenia species co-occur. A high prevalence of T. saginata taeniosis and bovine cysticercosis may reflect insufficiencies in sanitation, limited health education standards, and insufficient food safety measures. Therefore, there is a need to improve local surveillance, notification, and overall control systems.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia
IS  - 1
SP  - 234
VL  - 13
DO  - 10.1186/s13071-020-04095-1
ER  - 

@article{
author = "Eichenberger, Ramon M. and Thomas, Lian F. and Gabriel, Sarah and Bobić, Branko and Devleesschauwer, Brecht and Robertson, Lucy J. and Saratsis, Anastasios and Torgerson, Paul R. and Braae, Uffe C. and Dermauw, Veronique and Dorny, Pierre",
year = "2020",
abstract = "BackgroundTaenia saginata is an important zoonotic parasite, causing taeniosis in humans and cysticercosis in bovines, the latter being a significant concern for the global beef industry. Many countries in East, Southeast and South Asia are experiencing rapid economic growth, and an increasing number of people in these countries are dependent on the livestock industry. Currently, however, an overview of the prevalence of T. saginata in this region is lacking. In this review, we analysed the available literature on T. saginata taeniosis and bovine cysticercosis for East, Southeast and South Asia.MethodsA systematic review was conducted, based on both published and grey literature. Articles published between 1990 and 2017 were mined for information on the occurrence, prevalence, and geographical distribution of T. saginata taeniosis and bovine cysticercosis in East, Southeast and South Asia.ResultsThe presence of T. saginata was described in 15 of 27 countries of the region, including Afghanistan, Cambodia, China, India, Indonesia, Japan, Lao PDR, Malaysia, Mongolia, Nepal, Pakistan, Philippines, South Korea, Thailand and Vietnam. The only country that reported an absence of T. saginata is Japan, although sporadic reports of imported cases and unconfirmed reports of autochthonous infections were identified. Nationwide surveys of taeniosis with systematic sample collection and high sample numbers were available for Cambodia, China, Lao PDR, and South Korea, although speciation of Taenia was not always performed. Regional prevalence of taeniosis and bovine cysticercosis in endemic regions ranged between 0.02-42.6%, and 0.76-46.7%, respectively. However, data for bovine cysticercosis were only available for five countries (Japan, Lao PDR, Mongolia, Pakistan and Vietnam).ConclusionsThe data indicate a widespread occurrence of T. saginata throughout East, Southeast and South Asia. Identification of Taenia spp. in human infections was frequently not performed, leading to gaps in knowledge about the distribution of human tapeworm infections, mainly in regions where different human Taenia species co-occur. A high prevalence of T. saginata taeniosis and bovine cysticercosis may reflect insufficiencies in sanitation, limited health education standards, and insufficient food safety measures. Therefore, there is a need to improve local surveillance, notification, and overall control systems.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia",
number = "1",
pages = "234",
volume = "13",
doi = "10.1186/s13071-020-04095-1"
}

Eichenberger, R. M., Thomas, L. F., Gabriel, S., Bobić, B., Devleesschauwer, B., Robertson, L. J., Saratsis, A., Torgerson, P. R., Braae, U. C., Dermauw, V.,& Dorny, P.. (2020). Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia. in Parasites & Vectors
BMC, London., 13(1), 234.
https://doi.org/10.1186/s13071-020-04095-1

Eichenberger RM, Thomas LF, Gabriel S, Bobić B, Devleesschauwer B, Robertson LJ, Saratsis A, Torgerson PR, Braae UC, Dermauw V, Dorny P. Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia. in Parasites & Vectors. 2020;13(1):234.
doi:10.1186/s13071-020-04095-1 .

Eichenberger, Ramon M., Thomas, Lian F., Gabriel, Sarah, Bobić, Branko, Devleesschauwer, Brecht, Robertson, Lucy J., Saratsis, Anastasios, Torgerson, Paul R., Braae, Uffe C., Dermauw, Veronique, Dorny, Pierre, "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in East, Southeast and South Asia" in Parasites & Vectors, 13, no. 1 (2020):234,
https://doi.org/10.1186/s13071-020-04095-1 . .

TY  - JOUR
AU  - Stopić, Milena
AU  - Bobić, Branko
AU  - Dakić, Zorica
AU  - Srbljanović, Jelena
AU  - Štajner, Tijana
AU  - Konstantinović, Neda M.
AU  - Srećković, Katarina
AU  - Klun, Ivana
AU  - Korac, Miloš
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/909
AB  - Objectives: As is the case for all of Southeast Europe, Serbia is an area traditionally endemic for Taenia saginata and Taenia solium infections. This study was performed to analyse the epidemiological data on taeniosis and cysticercosis in Serbia for the period 1990-2018. Methods: Data on cases of T. saginata and T. solium infection were collected via a systematic search of published articles, the grey literature, and official reports, as well as by performing clinical observational studies of patients treated in the departments for infectious diseases of hospitals and university clinics in Serbia. Results: A total of 212 cases of taeniosis were reported, all between 1997 and 2004 when taeniosis was notifiable (incidence range 0.04-0.9/100 000 population/year). From 1990 to 2018, 170 cases of cysticercosis (all but one of neurocysticercosis), were registered (incidence range 0-0.29/100 000 population/year), with a strong decrease since 2000 and a single case in the last 9 years. The annual number of cases of both taeniosis (Pearson's r = 0.914, p = 0.001) and cysticercosis (Pearson's r = 0.582, p = 0.014) correlated with the consumer price index. Conclusions: In Serbia, T. saginata and T. solium infections are autochthonous but occur only sporadically. However, the potential for re-emergence exists, depending on the socio-economic state of the country.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Journal of Infectious Diseases
T1  - Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living
EP  - 141
SP  - 135
VL  - 86
DO  - 10.1016/j.ijid.2019.07.010
ER  - 

@article{
author = "Stopić, Milena and Bobić, Branko and Dakić, Zorica and Srbljanović, Jelena and Štajner, Tijana and Konstantinović, Neda M. and Srećković, Katarina and Klun, Ivana and Korac, Miloš and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Objectives: As is the case for all of Southeast Europe, Serbia is an area traditionally endemic for Taenia saginata and Taenia solium infections. This study was performed to analyse the epidemiological data on taeniosis and cysticercosis in Serbia for the period 1990-2018. Methods: Data on cases of T. saginata and T. solium infection were collected via a systematic search of published articles, the grey literature, and official reports, as well as by performing clinical observational studies of patients treated in the departments for infectious diseases of hospitals and university clinics in Serbia. Results: A total of 212 cases of taeniosis were reported, all between 1997 and 2004 when taeniosis was notifiable (incidence range 0.04-0.9/100 000 population/year). From 1990 to 2018, 170 cases of cysticercosis (all but one of neurocysticercosis), were registered (incidence range 0-0.29/100 000 population/year), with a strong decrease since 2000 and a single case in the last 9 years. The annual number of cases of both taeniosis (Pearson's r = 0.914, p = 0.001) and cysticercosis (Pearson's r = 0.582, p = 0.014) correlated with the consumer price index. Conclusions: In Serbia, T. saginata and T. solium infections are autochthonous but occur only sporadically. However, the potential for re-emergence exists, depending on the socio-economic state of the country.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Journal of Infectious Diseases",
title = "Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living",
pages = "141-135",
volume = "86",
doi = "10.1016/j.ijid.2019.07.010"
}

Stopić, M., Bobić, B., Dakić, Z., Srbljanović, J., Štajner, T., Konstantinović, N. M., Srećković, K., Klun, I., Korac, M.,& Đurković-Đaković, O.. (2019). Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living. in International Journal of Infectious Diseases
Elsevier Sci Ltd, Oxford., 86, 135-141.
https://doi.org/10.1016/j.ijid.2019.07.010

Stopić M, Bobić B, Dakić Z, Srbljanović J, Štajner T, Konstantinović NM, Srećković K, Klun I, Korac M, Đurković-Đaković O. Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living. in International Journal of Infectious Diseases. 2019;86:135-141.
doi:10.1016/j.ijid.2019.07.010 .

Stopić, Milena, Bobić, Branko, Dakić, Zorica, Srbljanović, Jelena, Štajner, Tijana, Konstantinović, Neda M., Srećković, Katarina, Klun, Ivana, Korac, Miloš, Đurković-Đaković, Olgica, "Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living" in International Journal of Infectious Diseases, 86 (2019):135-141,
https://doi.org/10.1016/j.ijid.2019.07.010 . .

TY  - JOUR
AU  - Hendrickx, Emilie
AU  - Thomas, Lian F.
AU  - Dorny, Pierre
AU  - Bobić, Branko
AU  - Braae, Uffe C.
AU  - Devleesschauwer, Brecht
AU  - Eichenberger, Ramon M.
AU  - Gabriel, Sarah
AU  - Saratsis, Anastasios
AU  - Torgerson, Paul R.
AU  - Robertson, Lucy J.
AU  - Dermauw, Veronique
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/924
AB  - Background: The zoonotic tapeworm Taenia saginata, although causing only minor discomfort in humans, is responsible for considerable economic losses in the livestock sector due to condemnation or downgrading of infected beef carcasses. An overview of current knowledge on the distribution and prevalence of this parasite in West and Central Africa is lacking. Methods: We conducted a systematic review, collecting information on published and grey literature about T. saginata taeniosis and bovine cysticercosis from 27 countries/territories in West and Central Africa, published between January 1st, 1990 and December 31st, 2017. Results: The literature search retrieved 1672 records, of which 51 and 45 were retained for a qualitative and quantitative synthesis, respectively. Non-specified human taeniosis cases were described for Nigeria, Cameroon, Senegal, Burkina Faso, Democratic Republic Congo, Guinea, and Ivory Coast (seven out of 27 countries/territories), while T. saginata taeniosis specifically was only reported for Cameroon. Most prevalence estimates for taeniosis ranged between 0-11%, while three studies from Nigeria reported prevalence estimates ranging between 23-50%. None of the studies included molecular confirmation of the causative species. The presence of bovine cysticercosis was reported for Benin, Burkina Faso, Cameroon, Central African Republic, Chad, Democratic Republic Congo, Ghana, Guinea, Ivory Coast, Mali, Niger, Nigeria, Senegal, and Tristan da Cunha (14 out of 27 countries/territories). Prevalence estimates ranged between 0-29%. Conclusions: Our systematic review has revealed that human taeniosis and bovine cysticercosis are seriously understudied in West and Central Africa. The high prevalence estimates of both conditions suggest an active dissemination of this parasite in the region, calling for a concerted One Health action from public health, veterinary health and food surveillance sectors.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in West and Central Africa
VL  - 12
DO  - 10.1186/s13071-019-3584-7
ER  - 

@article{
author = "Hendrickx, Emilie and Thomas, Lian F. and Dorny, Pierre and Bobić, Branko and Braae, Uffe C. and Devleesschauwer, Brecht and Eichenberger, Ramon M. and Gabriel, Sarah and Saratsis, Anastasios and Torgerson, Paul R. and Robertson, Lucy J. and Dermauw, Veronique",
year = "2019",
abstract = "Background: The zoonotic tapeworm Taenia saginata, although causing only minor discomfort in humans, is responsible for considerable economic losses in the livestock sector due to condemnation or downgrading of infected beef carcasses. An overview of current knowledge on the distribution and prevalence of this parasite in West and Central Africa is lacking. Methods: We conducted a systematic review, collecting information on published and grey literature about T. saginata taeniosis and bovine cysticercosis from 27 countries/territories in West and Central Africa, published between January 1st, 1990 and December 31st, 2017. Results: The literature search retrieved 1672 records, of which 51 and 45 were retained for a qualitative and quantitative synthesis, respectively. Non-specified human taeniosis cases were described for Nigeria, Cameroon, Senegal, Burkina Faso, Democratic Republic Congo, Guinea, and Ivory Coast (seven out of 27 countries/territories), while T. saginata taeniosis specifically was only reported for Cameroon. Most prevalence estimates for taeniosis ranged between 0-11%, while three studies from Nigeria reported prevalence estimates ranging between 23-50%. None of the studies included molecular confirmation of the causative species. The presence of bovine cysticercosis was reported for Benin, Burkina Faso, Cameroon, Central African Republic, Chad, Democratic Republic Congo, Ghana, Guinea, Ivory Coast, Mali, Niger, Nigeria, Senegal, and Tristan da Cunha (14 out of 27 countries/territories). Prevalence estimates ranged between 0-29%. Conclusions: Our systematic review has revealed that human taeniosis and bovine cysticercosis are seriously understudied in West and Central Africa. The high prevalence estimates of both conditions suggest an active dissemination of this parasite in the region, calling for a concerted One Health action from public health, veterinary health and food surveillance sectors.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in West and Central Africa",
volume = "12",
doi = "10.1186/s13071-019-3584-7"
}

Hendrickx, E., Thomas, L. F., Dorny, P., Bobić, B., Braae, U. C., Devleesschauwer, B., Eichenberger, R. M., Gabriel, S., Saratsis, A., Torgerson, P. R., Robertson, L. J.,& Dermauw, V.. (2019). Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in West and Central Africa. in Parasites & Vectors
BMC, London., 12.
https://doi.org/10.1186/s13071-019-3584-7

Hendrickx E, Thomas LF, Dorny P, Bobić B, Braae UC, Devleesschauwer B, Eichenberger RM, Gabriel S, Saratsis A, Torgerson PR, Robertson LJ, Dermauw V. Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in West and Central Africa. in Parasites & Vectors. 2019;12.
doi:10.1186/s13071-019-3584-7 .

Hendrickx, Emilie, Thomas, Lian F., Dorny, Pierre, Bobić, Branko, Braae, Uffe C., Devleesschauwer, Brecht, Eichenberger, Ramon M., Gabriel, Sarah, Saratsis, Anastasios, Torgerson, Paul R., Robertson, Lucy J., Dermauw, Veronique, "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in West and Central Africa" in Parasites & Vectors, 12 (2019),
https://doi.org/10.1186/s13071-019-3584-7 . .

TY  - JOUR
AU  - Saratsis, Anastasios
AU  - Sotiraki, Smaragda
AU  - Braae, Uffe C.
AU  - Devleesschauwer, Brecht
AU  - Dermauw, Veronique
AU  - Eichenberger, Ramon M.
AU  - Thomas, Lian F.
AU  - Bobić, Branko
AU  - Dorny, Pierre
AU  - Gabriel, Sarah
AU  - Robertson, Lucy J.
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/942
AB  - BackgroundThe zoonotic parasite Taenia saginata utilizes bovines as an intermediate host (causing cysticercosis) and humans as the definitive host (causing taeniosis). The public health burden of T. saginata is assumed to be low, but the economic burden is large, due to the resources utilized in the detection and condemnation of infected carcasses and carcass parts. As part of a collaborative effort to synthesize worldwide epidemiological data on this parasite, we present here the results of a systematic review on the distribution of T. saginata taeniosis and bovine cysticercosis in the Middle East and North Africa (MENA).MethodsInformation on the occurrence and prevalence of T. saginata taeniosis and cysticercosis in the MENA region was obtained through a systematic review of published and grey literature, including OIE reports, published between January 1st, 1990 and December 31st, 2017.ResultsA total of 63 publications were retrieved across the 21 MENA countries. Taenia saginata taeniosis was reported in 11 of these countries, whereas unspecified taeniosis was reported for a further seven. Microscopy-based prevalence values ranged between 0.02-8.6%. Bovine cysticercosis prevalence estimates based on meat inspection were only reported for Egypt and Israel, with prevalence data ranging between 0.2-20% and 0.1-9.1% for cattle and buffaloes, respectively. The presence of bovine cysticercosis could be confirmed for 10 additional countries through OIE reports.ConclusionsHuman taeniosis occurrence was confirmed for 86% (18/21) of the countries in the MENA region, although in several of these countries the species responsible was not specified. Religious prohibitions on the consumption of pork and the limited extent of pig farming across much of this region, however, suggest that many reported taeniosis cases are likely to be attributable to T. saginata rather than Taenia solium or Taenia asiatica. There was a paucity of data regarding both the prevalence and economic impact of bovine cysticercosis. More detailed epidemiological data on both T. saginata taeniosis and bovine cysticercosis could be obtained by adopting an integrated One Health approach, considering the characteristics (e.g. ecosystem related and sociopolitical aspects) of the MENA region. Compared with more conventional approaches, this could lead to an enhanced performance and cost-effectiveness of surveillance systems.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in the Middle East and North Africa
VL  - 12
DO  - 10.1186/s13071-019-3339-5
ER  - 

@article{
author = "Saratsis, Anastasios and Sotiraki, Smaragda and Braae, Uffe C. and Devleesschauwer, Brecht and Dermauw, Veronique and Eichenberger, Ramon M. and Thomas, Lian F. and Bobić, Branko and Dorny, Pierre and Gabriel, Sarah and Robertson, Lucy J.",
year = "2019",
abstract = "BackgroundThe zoonotic parasite Taenia saginata utilizes bovines as an intermediate host (causing cysticercosis) and humans as the definitive host (causing taeniosis). The public health burden of T. saginata is assumed to be low, but the economic burden is large, due to the resources utilized in the detection and condemnation of infected carcasses and carcass parts. As part of a collaborative effort to synthesize worldwide epidemiological data on this parasite, we present here the results of a systematic review on the distribution of T. saginata taeniosis and bovine cysticercosis in the Middle East and North Africa (MENA).MethodsInformation on the occurrence and prevalence of T. saginata taeniosis and cysticercosis in the MENA region was obtained through a systematic review of published and grey literature, including OIE reports, published between January 1st, 1990 and December 31st, 2017.ResultsA total of 63 publications were retrieved across the 21 MENA countries. Taenia saginata taeniosis was reported in 11 of these countries, whereas unspecified taeniosis was reported for a further seven. Microscopy-based prevalence values ranged between 0.02-8.6%. Bovine cysticercosis prevalence estimates based on meat inspection were only reported for Egypt and Israel, with prevalence data ranging between 0.2-20% and 0.1-9.1% for cattle and buffaloes, respectively. The presence of bovine cysticercosis could be confirmed for 10 additional countries through OIE reports.ConclusionsHuman taeniosis occurrence was confirmed for 86% (18/21) of the countries in the MENA region, although in several of these countries the species responsible was not specified. Religious prohibitions on the consumption of pork and the limited extent of pig farming across much of this region, however, suggest that many reported taeniosis cases are likely to be attributable to T. saginata rather than Taenia solium or Taenia asiatica. There was a paucity of data regarding both the prevalence and economic impact of bovine cysticercosis. More detailed epidemiological data on both T. saginata taeniosis and bovine cysticercosis could be obtained by adopting an integrated One Health approach, considering the characteristics (e.g. ecosystem related and sociopolitical aspects) of the MENA region. Compared with more conventional approaches, this could lead to an enhanced performance and cost-effectiveness of surveillance systems.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in the Middle East and North Africa",
volume = "12",
doi = "10.1186/s13071-019-3339-5"
}

Saratsis, A., Sotiraki, S., Braae, U. C., Devleesschauwer, B., Dermauw, V., Eichenberger, R. M., Thomas, L. F., Bobić, B., Dorny, P., Gabriel, S.,& Robertson, L. J.. (2019). Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in the Middle East and North Africa. in Parasites & Vectors
BMC, London., 12.
https://doi.org/10.1186/s13071-019-3339-5

Saratsis A, Sotiraki S, Braae UC, Devleesschauwer B, Dermauw V, Eichenberger RM, Thomas LF, Bobić B, Dorny P, Gabriel S, Robertson LJ. Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in the Middle East and North Africa. in Parasites & Vectors. 2019;12.
doi:10.1186/s13071-019-3339-5 .

Saratsis, Anastasios, Sotiraki, Smaragda, Braae, Uffe C., Devleesschauwer, Brecht, Dermauw, Veronique, Eichenberger, Ramon M., Thomas, Lian F., Bobić, Branko, Dorny, Pierre, Gabriel, Sarah, Robertson, Lucy J., "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in the Middle East and North Africa" in Parasites & Vectors, 12 (2019),
https://doi.org/10.1186/s13071-019-3339-5 . .

TY  - JOUR
AU  - Bobić, Branko
AU  - Villena, Isabelle
AU  - Stillwaggon, E.
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/951
AB  - Congenital toxoplasmosis (CT), the result of a primary infection of pregnant women with Toxoplasma gondii which was transmitted to the fetus, may result in mild to deep injuries occurring in the newborn or later in its development or in adolescence. The visual and cognitive impairment that can result imposes substantial economic costs on the individual and society. Numerous observational studies favor the conclusion that, with preventive measures currently available, it is possible to reduce the incidence of infections in pregnant women, the incidence of fetal infection by preventing transplacental transmission, and the gravity of injury in infected newborns. Treatment of infected newborns can also reduce the severity of consequences and the frequency of their occurrence later in life. Prevention programs, however, are applied in only a few countries; in most countries implementation of a national prevention program has not been considered or has been thought to be too expensive. This article lists the methods of prevention of CT and describes existing national prevention programs in France and Austria. It analyzes the economic costs and benefits of maternal screening for CT prevention and mitigation for society and for health systems. The economic feasibility of implementing national screening in low-prevalence, high-cost countries is illustrated with the example of the United States. New diagnostic tools are discussed and the implication of lower costs is considered, for countries with well-established screening programs as well as those with inadequate prenatal care networks.
T2  - Food & Waterborne Parasitology
T1  - Prevention and mitigation of congenital toxoplasmosis. Economic costs and benefits in diverse settings
VL  - 16
DO  - 10.1016/j.fawpar.2019.e00058
ER  - 

@article{
author = "Bobić, Branko and Villena, Isabelle and Stillwaggon, E.",
year = "2019",
abstract = "Congenital toxoplasmosis (CT), the result of a primary infection of pregnant women with Toxoplasma gondii which was transmitted to the fetus, may result in mild to deep injuries occurring in the newborn or later in its development or in adolescence. The visual and cognitive impairment that can result imposes substantial economic costs on the individual and society. Numerous observational studies favor the conclusion that, with preventive measures currently available, it is possible to reduce the incidence of infections in pregnant women, the incidence of fetal infection by preventing transplacental transmission, and the gravity of injury in infected newborns. Treatment of infected newborns can also reduce the severity of consequences and the frequency of their occurrence later in life. Prevention programs, however, are applied in only a few countries; in most countries implementation of a national prevention program has not been considered or has been thought to be too expensive. This article lists the methods of prevention of CT and describes existing national prevention programs in France and Austria. It analyzes the economic costs and benefits of maternal screening for CT prevention and mitigation for society and for health systems. The economic feasibility of implementing national screening in low-prevalence, high-cost countries is illustrated with the example of the United States. New diagnostic tools are discussed and the implication of lower costs is considered, for countries with well-established screening programs as well as those with inadequate prenatal care networks.",
journal = "Food & Waterborne Parasitology",
title = "Prevention and mitigation of congenital toxoplasmosis. Economic costs and benefits in diverse settings",
volume = "16",
doi = "10.1016/j.fawpar.2019.e00058"
}

Bobić, B., Villena, I.,& Stillwaggon, E.. (2019). Prevention and mitigation of congenital toxoplasmosis. Economic costs and benefits in diverse settings. in Food & Waterborne Parasitology, 16.
https://doi.org/10.1016/j.fawpar.2019.e00058

Bobić B, Villena I, Stillwaggon E. Prevention and mitigation of congenital toxoplasmosis. Economic costs and benefits in diverse settings. in Food & Waterborne Parasitology. 2019;16.
doi:10.1016/j.fawpar.2019.e00058 .

Bobić, Branko, Villena, Isabelle, Stillwaggon, E., "Prevention and mitigation of congenital toxoplasmosis. Economic costs and benefits in diverse settings" in Food & Waterborne Parasitology, 16 (2019),
https://doi.org/10.1016/j.fawpar.2019.e00058 . .

TY  - JOUR
AU  - Trevisan, Chiara
AU  - Sotiraki, Smaragda
AU  - Laranjo-Gonzalez, Minerva
AU  - Dermauw, Veronique
AU  - Wang, Ziqi
AU  - Kaerssin, Age
AU  - Cvetkovikj, Aleksandar
AU  - Winkler, Andrea S.
AU  - Abraham, Annette
AU  - Bobić, Branko
AU  - Lassen, Brian
AU  - Cretu, Carmen Michaela
AU  - Vasile, Cozma
AU  - Arvanitis, Dimitris
AU  - Deksne, Gunita
AU  - Ilievski, Boro
AU  - Kucsera, Istvan
AU  - Karamon, Jacek
AU  - Stefanovska, Jovana
AU  - Koudela, Bretislav
AU  - Jurhar-Pavlova, Maja
AU  - Varady, Marian
AU  - Pavlak, Marina
AU  - Sarkunas, Mindaugas
AU  - Kaminski, Miriam
AU  - Đurković-Đaković, Olgica
AU  - Jokelainen, Pikka
AU  - Jan, Dagny Stojcevic
AU  - Schmidt, Veronika
AU  - Dakić, Zorica
AU  - Gabriel, Sarah
AU  - Dorny, Pierre
AU  - Devleesschauwer, Brecht
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/963
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018)
VL  - 12
DO  - 10.1186/s13071-019-3328-8
ER  - 

@article{
author = "Trevisan, Chiara and Sotiraki, Smaragda and Laranjo-Gonzalez, Minerva and Dermauw, Veronique and Wang, Ziqi and Kaerssin, Age and Cvetkovikj, Aleksandar and Winkler, Andrea S. and Abraham, Annette and Bobić, Branko and Lassen, Brian and Cretu, Carmen Michaela and Vasile, Cozma and Arvanitis, Dimitris and Deksne, Gunita and Ilievski, Boro and Kucsera, Istvan and Karamon, Jacek and Stefanovska, Jovana and Koudela, Bretislav and Jurhar-Pavlova, Maja and Varady, Marian and Pavlak, Marina and Sarkunas, Mindaugas and Kaminski, Miriam and Đurković-Đaković, Olgica and Jokelainen, Pikka and Jan, Dagny Stojcevic and Schmidt, Veronika and Dakić, Zorica and Gabriel, Sarah and Dorny, Pierre and Devleesschauwer, Brecht",
year = "2019",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018)",
volume = "12",
doi = "10.1186/s13071-019-3328-8"
}

Trevisan, C., Sotiraki, S., Laranjo-Gonzalez, M., Dermauw, V., Wang, Z., Kaerssin, A., Cvetkovikj, A., Winkler, A. S., Abraham, A., Bobić, B., Lassen, B., Cretu, C. M., Vasile, C., Arvanitis, D., Deksne, G., Ilievski, B., Kucsera, I., Karamon, J., Stefanovska, J., Koudela, B., Jurhar-Pavlova, M., Varady, M., Pavlak, M., Sarkunas, M., Kaminski, M., Đurković-Đaković, O., Jokelainen, P., Jan, D. S., Schmidt, V., Dakić, Z., Gabriel, S., Dorny, P.,& Devleesschauwer, B.. (2019). Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018). in Parasites & Vectors
BMC, London., 12.
https://doi.org/10.1186/s13071-019-3328-8

Trevisan C, Sotiraki S, Laranjo-Gonzalez M, Dermauw V, Wang Z, Kaerssin A, Cvetkovikj A, Winkler AS, Abraham A, Bobić B, Lassen B, Cretu CM, Vasile C, Arvanitis D, Deksne G, Ilievski B, Kucsera I, Karamon J, Stefanovska J, Koudela B, Jurhar-Pavlova M, Varady M, Pavlak M, Sarkunas M, Kaminski M, Đurković-Đaković O, Jokelainen P, Jan DS, Schmidt V, Dakić Z, Gabriel S, Dorny P, Devleesschauwer B. Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018). in Parasites & Vectors. 2019;12.
doi:10.1186/s13071-019-3328-8 .

Trevisan, Chiara, Sotiraki, Smaragda, Laranjo-Gonzalez, Minerva, Dermauw, Veronique, Wang, Ziqi, Kaerssin, Age, Cvetkovikj, Aleksandar, Winkler, Andrea S., Abraham, Annette, Bobić, Branko, Lassen, Brian, Cretu, Carmen Michaela, Vasile, Cozma, Arvanitis, Dimitris, Deksne, Gunita, Ilievski, Boro, Kucsera, Istvan, Karamon, Jacek, Stefanovska, Jovana, Koudela, Bretislav, Jurhar-Pavlova, Maja, Varady, Marian, Pavlak, Marina, Sarkunas, Mindaugas, Kaminski, Miriam, Đurković-Đaković, Olgica, Jokelainen, Pikka, Jan, Dagny Stojcevic, Schmidt, Veronika, Dakić, Zorica, Gabriel, Sarah, Dorny, Pierre, Devleesschauwer, Brecht, "Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018)" in Parasites & Vectors, 12 (2019),
https://doi.org/10.1186/s13071-019-3328-8 . .

TY  - JOUR
AU  - Torgerson, Paul R.
AU  - Abdybekova, Aida M.
AU  - Minbaeva, Gulnara
AU  - Shapiyeva, Zhanna
AU  - Thomas, Lian F.
AU  - Dermauw, Veronique
AU  - Devleesschauwer, Brecht
AU  - Gabriel, Sarah
AU  - Dorny, Pierre
AU  - Braae, Uffe C.
AU  - Saratsis, Anastasios
AU  - Robertson, Lucy J.
AU  - Bobić, Branko
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/970
AB  - BackgroundThe zoonotic parasite Taenia saginata transmits between humans, the definitive host (causing taeniosis), and bovines as the intermediate host (causing cysticercosis). Central and western Asia and the Caucasus have large cattle populations and beef consumption is widespread. However, an overview of the extent of human T. saginata infection and bovine cysticercosis is lacking. This review aims to summarize the distribution of T. saginata in this region.MethodsA systematic review was conducted, that gathered published and grey literature, and official data concerning T. saginata taeniosis and bovine cysticercosis in central and western Asia and the Caucasus published between January 1st, 1990 and December 31st, 2018. Where no data were available for a country within this period, published data from 1985-1990 were also accessed.ResultsFrom 10,786 articles initially scanned, we retrieved 98 full-text articles from which data were extracted. In addition, two unpublished datasets were provided on the incidence of human taeniosis. Data for human taeniosis and bovine cysticercosis were found for all countries except Turkmenistan. Human taeniosis prevalence varied from undetected to over 5.3%, with regional variations. Where bovine cysticercosis was detected, prevalences varied from case reports to 25%.ConclusionsThe public health burden of T. saginata is assumed to be small as the parasite is of low pathogenicity to humans. However, this review indicates that infection continues to be widespread and this may result in a large economic burden, due to the resources utilized in meat inspection and condemnation or processing with subsequent downgrading of infected carcasses.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in central and western Asia and the Caucasus
VL  - 12
DO  - 10.1186/s13071-019-3438-3
ER  - 

@article{
author = "Torgerson, Paul R. and Abdybekova, Aida M. and Minbaeva, Gulnara and Shapiyeva, Zhanna and Thomas, Lian F. and Dermauw, Veronique and Devleesschauwer, Brecht and Gabriel, Sarah and Dorny, Pierre and Braae, Uffe C. and Saratsis, Anastasios and Robertson, Lucy J. and Bobić, Branko",
year = "2019",
abstract = "BackgroundThe zoonotic parasite Taenia saginata transmits between humans, the definitive host (causing taeniosis), and bovines as the intermediate host (causing cysticercosis). Central and western Asia and the Caucasus have large cattle populations and beef consumption is widespread. However, an overview of the extent of human T. saginata infection and bovine cysticercosis is lacking. This review aims to summarize the distribution of T. saginata in this region.MethodsA systematic review was conducted, that gathered published and grey literature, and official data concerning T. saginata taeniosis and bovine cysticercosis in central and western Asia and the Caucasus published between January 1st, 1990 and December 31st, 2018. Where no data were available for a country within this period, published data from 1985-1990 were also accessed.ResultsFrom 10,786 articles initially scanned, we retrieved 98 full-text articles from which data were extracted. In addition, two unpublished datasets were provided on the incidence of human taeniosis. Data for human taeniosis and bovine cysticercosis were found for all countries except Turkmenistan. Human taeniosis prevalence varied from undetected to over 5.3%, with regional variations. Where bovine cysticercosis was detected, prevalences varied from case reports to 25%.ConclusionsThe public health burden of T. saginata is assumed to be small as the parasite is of low pathogenicity to humans. However, this review indicates that infection continues to be widespread and this may result in a large economic burden, due to the resources utilized in meat inspection and condemnation or processing with subsequent downgrading of infected carcasses.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in central and western Asia and the Caucasus",
volume = "12",
doi = "10.1186/s13071-019-3438-3"
}

Torgerson, P. R., Abdybekova, A. M., Minbaeva, G., Shapiyeva, Z., Thomas, L. F., Dermauw, V., Devleesschauwer, B., Gabriel, S., Dorny, P., Braae, U. C., Saratsis, A., Robertson, L. J.,& Bobić, B.. (2019). Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in central and western Asia and the Caucasus. in Parasites & Vectors
BMC, London., 12.
https://doi.org/10.1186/s13071-019-3438-3

Torgerson PR, Abdybekova AM, Minbaeva G, Shapiyeva Z, Thomas LF, Dermauw V, Devleesschauwer B, Gabriel S, Dorny P, Braae UC, Saratsis A, Robertson LJ, Bobić B. Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in central and western Asia and the Caucasus. in Parasites & Vectors. 2019;12.
doi:10.1186/s13071-019-3438-3 .

Torgerson, Paul R., Abdybekova, Aida M., Minbaeva, Gulnara, Shapiyeva, Zhanna, Thomas, Lian F., Dermauw, Veronique, Devleesschauwer, Brecht, Gabriel, Sarah, Dorny, Pierre, Braae, Uffe C., Saratsis, Anastasios, Robertson, Lucy J., Bobić, Branko, "Epidemiology of Taenia saginata taeniosis/cysticercosis: a systematic review of the distribution in central and western Asia and the Caucasus" in Parasites & Vectors, 12 (2019),
https://doi.org/10.1186/s13071-019-3438-3 . .

TY  - JOUR
AU  - Bobić, Branko
AU  - Thomas, Lian F.
AU  - Đurković-Đaković, Olgica
AU  - Devleesschauwer, Brecht
AU  - Dermauw, Veronique
AU  - Dorny, Pierre
AU  - Braae, Uffe C.
AU  - Robertson, Lucy J.
AU  - Saratsis, Anastasios
AU  - Eichenberger, Ramon M.
AU  - Torgerson, Paul R.
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/851
AB  - BackgroundRussia is traditionally an endemic area for Taenia saginata infection, where a programme for the prevention of infection has been implemented for sixty years. This paper aims, therefore, to review the recent epidemiology data of Taenia saginata infection in the Russian Federation.MethodsWe undertook a systematic review of published and grey literature, and official data for information on the incidence, prevalence and distribution of Taenia saginata taeniosis and cysticercosis in the Russian Federation between 1st January 1991 and 31st December 2017.ResultsFrom the 404 records returned by our search strategy, we identified 17 official county reports, 17 papers and one meeting abstract on the occurrence of taeniosis or cysticercosis from the Russian Federation, eligible for inclusion in this study. In the Russian Federation, Taenia saginata infection has been continuously present and notifiable in the study period between 1991-2016. In the same area, a continuous decrease in the incidence of human taeniosis cases was observed, from 1.4 to 0.04 cases per 100,000 inhabitants, as well as a reduction in the territory where the infection is reported. The prevalence of bovine cysticercosis, ranging between 0.1-19.0%, generally has a declining trend, especially after 2005.ConclusionsImportance of Taenia saginata infection as a medical and veterinary problem has been decreasing in the 21st century but it is still an infection with health and economic impact in the Russian Federation.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of Taenia saginata taeniosis/cysticercosis in the Russian Federation
IS  - 1
SP  - 636
VL  - 11
DO  - 10.1186/s13071-018-3236-3
ER  - 

@article{
author = "Bobić, Branko and Thomas, Lian F. and Đurković-Đaković, Olgica and Devleesschauwer, Brecht and Dermauw, Veronique and Dorny, Pierre and Braae, Uffe C. and Robertson, Lucy J. and Saratsis, Anastasios and Eichenberger, Ramon M. and Torgerson, Paul R.",
year = "2018",
abstract = "BackgroundRussia is traditionally an endemic area for Taenia saginata infection, where a programme for the prevention of infection has been implemented for sixty years. This paper aims, therefore, to review the recent epidemiology data of Taenia saginata infection in the Russian Federation.MethodsWe undertook a systematic review of published and grey literature, and official data for information on the incidence, prevalence and distribution of Taenia saginata taeniosis and cysticercosis in the Russian Federation between 1st January 1991 and 31st December 2017.ResultsFrom the 404 records returned by our search strategy, we identified 17 official county reports, 17 papers and one meeting abstract on the occurrence of taeniosis or cysticercosis from the Russian Federation, eligible for inclusion in this study. In the Russian Federation, Taenia saginata infection has been continuously present and notifiable in the study period between 1991-2016. In the same area, a continuous decrease in the incidence of human taeniosis cases was observed, from 1.4 to 0.04 cases per 100,000 inhabitants, as well as a reduction in the territory where the infection is reported. The prevalence of bovine cysticercosis, ranging between 0.1-19.0%, generally has a declining trend, especially after 2005.ConclusionsImportance of Taenia saginata infection as a medical and veterinary problem has been decreasing in the 21st century but it is still an infection with health and economic impact in the Russian Federation.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of Taenia saginata taeniosis/cysticercosis in the Russian Federation",
number = "1",
pages = "636",
volume = "11",
doi = "10.1186/s13071-018-3236-3"
}

Bobić, B., Thomas, L. F., Đurković-Đaković, O., Devleesschauwer, B., Dermauw, V., Dorny, P., Braae, U. C., Robertson, L. J., Saratsis, A., Eichenberger, R. M.,& Torgerson, P. R.. (2018). Epidemiology of Taenia saginata taeniosis/cysticercosis in the Russian Federation. in Parasites & Vectors
BMC, London., 11(1), 636.
https://doi.org/10.1186/s13071-018-3236-3

Bobić B, Thomas LF, Đurković-Đaković O, Devleesschauwer B, Dermauw V, Dorny P, Braae UC, Robertson LJ, Saratsis A, Eichenberger RM, Torgerson PR. Epidemiology of Taenia saginata taeniosis/cysticercosis in the Russian Federation. in Parasites & Vectors. 2018;11(1):636.
doi:10.1186/s13071-018-3236-3 .

Bobić, Branko, Thomas, Lian F., Đurković-Đaković, Olgica, Devleesschauwer, Brecht, Dermauw, Veronique, Dorny, Pierre, Braae, Uffe C., Robertson, Lucy J., Saratsis, Anastasios, Eichenberger, Ramon M., Torgerson, Paul R., "Epidemiology of Taenia saginata taeniosis/cysticercosis in the Russian Federation" in Parasites & Vectors, 11, no. 1 (2018):636,
https://doi.org/10.1186/s13071-018-3236-3 . .

TY  - JOUR
AU  - Trevisan, Chiara
AU  - Sotiraki, Smaragda
AU  - Laranjo-Gonzalez, Minerva
AU  - Dermauw, Veronique
AU  - Wang, Ziqi
AU  - Kaerssin, Age
AU  - Cvetkovikj, Aleksandar
AU  - Winkler, Andrea S.
AU  - Abraham, Annette
AU  - Bobić, Branko
AU  - Lassen, Brian
AU  - Cretu, Carmen Michaela
AU  - Vasile, Cozma
AU  - Arvanitis, Dimitris
AU  - Deksne, Gunita
AU  - Ilievski, Boro
AU  - Kucsera, Istvan
AU  - Karamon, Jacek
AU  - Stefanovska, Jovana
AU  - Koudela, Bretislav
AU  - Jurhar-Pavlova, Maja
AU  - Varady, Marian
AU  - Pavlak, Marina
AU  - Sarkunas, Mindaugas
AU  - Kaminski, Miriam
AU  - Đurković-Đaković, Olgica
AU  - Jokelainen, Pikka
AU  - Jan, Dagny Stojcevic
AU  - Schmidt, Veronika
AU  - Dakić, Zorica
AU  - Gabriel, Sarah
AU  - Dorny, Pierre
AU  - Devleesschauwer, Brecht
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/856
AB  - Background: Taenia solium and Taenia saginata are food-borne parasites of global importance. In eastern Europe only fragmented information is available on the epidemiology of these zoonotic parasites in humans and animal populations. In particular for T. solium, on-going transmission is suspected. The aim of this systematic review was to collect the available data and describe the current knowledge on the epidemiology of T. solium and T. saginata in eastern Europe. Methods: Literature published in international databases from 1990 to 2017 was systematically reviewed. Furthermore, local sources and unpublished data from national databases were retrieved from local eastern European experts. The study area included 22 countries. Results: Researchers from 18 out of the 22 countries provided data from local and unpublished sources, while no contacts could be established with researchers from Belarus, Kosovo, Malta and Ukraine. Taeniosis and human cysticercosis cases were reported in 14 and 15 out of the 22 countries, respectively. Estonia, the Former Yugoslav Republic of Macedonia, Lithuania, Moldova, Poland, Romania, Serbia, and Slovakia reported cases of porcine cysticercosis. Croatia, Czech Republic, Estonia, Former Yugoslav Republic of Macedonia, Moldova, Poland, Romania, Serbia, Slovakia, and Ukraine reported bovine cysticercosis. Conclusions: There is indication that taeniosis and cysticercosis are present across eastern Europe but information on the occurrence of T. solium and T. saginata across the region remains incomplete. Available data are scarce and species identification is in most cases absent. Given the public health impact of T. solium and the potential economic and trade implications due to T. saginata, notification of taeniosis and human cysticercosis should be implemented and surveillance and notification systems in animals should be improved.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe
VL  - 11
DO  - 10.1186/s13071-018-3153-5
ER  - 

@article{
author = "Trevisan, Chiara and Sotiraki, Smaragda and Laranjo-Gonzalez, Minerva and Dermauw, Veronique and Wang, Ziqi and Kaerssin, Age and Cvetkovikj, Aleksandar and Winkler, Andrea S. and Abraham, Annette and Bobić, Branko and Lassen, Brian and Cretu, Carmen Michaela and Vasile, Cozma and Arvanitis, Dimitris and Deksne, Gunita and Ilievski, Boro and Kucsera, Istvan and Karamon, Jacek and Stefanovska, Jovana and Koudela, Bretislav and Jurhar-Pavlova, Maja and Varady, Marian and Pavlak, Marina and Sarkunas, Mindaugas and Kaminski, Miriam and Đurković-Đaković, Olgica and Jokelainen, Pikka and Jan, Dagny Stojcevic and Schmidt, Veronika and Dakić, Zorica and Gabriel, Sarah and Dorny, Pierre and Devleesschauwer, Brecht",
year = "2018",
abstract = "Background: Taenia solium and Taenia saginata are food-borne parasites of global importance. In eastern Europe only fragmented information is available on the epidemiology of these zoonotic parasites in humans and animal populations. In particular for T. solium, on-going transmission is suspected. The aim of this systematic review was to collect the available data and describe the current knowledge on the epidemiology of T. solium and T. saginata in eastern Europe. Methods: Literature published in international databases from 1990 to 2017 was systematically reviewed. Furthermore, local sources and unpublished data from national databases were retrieved from local eastern European experts. The study area included 22 countries. Results: Researchers from 18 out of the 22 countries provided data from local and unpublished sources, while no contacts could be established with researchers from Belarus, Kosovo, Malta and Ukraine. Taeniosis and human cysticercosis cases were reported in 14 and 15 out of the 22 countries, respectively. Estonia, the Former Yugoslav Republic of Macedonia, Lithuania, Moldova, Poland, Romania, Serbia, and Slovakia reported cases of porcine cysticercosis. Croatia, Czech Republic, Estonia, Former Yugoslav Republic of Macedonia, Moldova, Poland, Romania, Serbia, Slovakia, and Ukraine reported bovine cysticercosis. Conclusions: There is indication that taeniosis and cysticercosis are present across eastern Europe but information on the occurrence of T. solium and T. saginata across the region remains incomplete. Available data are scarce and species identification is in most cases absent. Given the public health impact of T. solium and the potential economic and trade implications due to T. saginata, notification of taeniosis and human cysticercosis should be implemented and surveillance and notification systems in animals should be improved.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe",
volume = "11",
doi = "10.1186/s13071-018-3153-5"
}

Trevisan, C., Sotiraki, S., Laranjo-Gonzalez, M., Dermauw, V., Wang, Z., Kaerssin, A., Cvetkovikj, A., Winkler, A. S., Abraham, A., Bobić, B., Lassen, B., Cretu, C. M., Vasile, C., Arvanitis, D., Deksne, G., Ilievski, B., Kucsera, I., Karamon, J., Stefanovska, J., Koudela, B., Jurhar-Pavlova, M., Varady, M., Pavlak, M., Sarkunas, M., Kaminski, M., Đurković-Đaković, O., Jokelainen, P., Jan, D. S., Schmidt, V., Dakić, Z., Gabriel, S., Dorny, P.,& Devleesschauwer, B.. (2018). Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe. in Parasites & Vectors
BMC, London., 11.
https://doi.org/10.1186/s13071-018-3153-5

Trevisan C, Sotiraki S, Laranjo-Gonzalez M, Dermauw V, Wang Z, Kaerssin A, Cvetkovikj A, Winkler AS, Abraham A, Bobić B, Lassen B, Cretu CM, Vasile C, Arvanitis D, Deksne G, Ilievski B, Kucsera I, Karamon J, Stefanovska J, Koudela B, Jurhar-Pavlova M, Varady M, Pavlak M, Sarkunas M, Kaminski M, Đurković-Đaković O, Jokelainen P, Jan DS, Schmidt V, Dakić Z, Gabriel S, Dorny P, Devleesschauwer B. Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe. in Parasites & Vectors. 2018;11.
doi:10.1186/s13071-018-3153-5 .

Trevisan, Chiara, Sotiraki, Smaragda, Laranjo-Gonzalez, Minerva, Dermauw, Veronique, Wang, Ziqi, Kaerssin, Age, Cvetkovikj, Aleksandar, Winkler, Andrea S., Abraham, Annette, Bobić, Branko, Lassen, Brian, Cretu, Carmen Michaela, Vasile, Cozma, Arvanitis, Dimitris, Deksne, Gunita, Ilievski, Boro, Kucsera, Istvan, Karamon, Jacek, Stefanovska, Jovana, Koudela, Bretislav, Jurhar-Pavlova, Maja, Varady, Marian, Pavlak, Marina, Sarkunas, Mindaugas, Kaminski, Miriam, Đurković-Đaković, Olgica, Jokelainen, Pikka, Jan, Dagny Stojcevic, Schmidt, Veronika, Dakić, Zorica, Gabriel, Sarah, Dorny, Pierre, Devleesschauwer, Brecht, "Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe" in Parasites & Vectors, 11 (2018),
https://doi.org/10.1186/s13071-018-3153-5 . .