dc.creator | Kukolj, Tamara | |
dc.creator | Lazarević, Jasmina | |
dc.creator | Borojević, Ana | |
dc.creator | Ralević, Uroš | |
dc.creator | Vujić, Dragana | |
dc.creator | Jauković, Aleksandra | |
dc.creator | Lazarević, Nenad | |
dc.creator | Bugarski, Diana | |
dc.date.accessioned | 2022-06-10T10:57:11Z | |
dc.date.available | 2022-06-10T10:57:11Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/1235 | |
dc.description.abstract | The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches. | |
dc.publisher | MDPI | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200015/RS// | |
dc.relation | "Start up for science” 2019/2020 - Center for Leadership Development and the Philip Morris Company in Serbia | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200024/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | International Journal of Molecular Sciences | |
dc.subject | human bone marrow mesenchymal stem/stromal cells (BM-MSCs) | |
dc.subject | Raman spectroscopy | |
dc.subject | single cell | |
dc.subject | inter-individual heterogeneity | |
dc.title | A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity | |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.issue | 9 | |
dc.citation.spage | 4915 | |
dc.citation.volume | 23 | |
dc.identifier.doi | 10.3390/ijms23094915 | |
dc.identifier.fulltext | http://rimi.imi.bg.ac.rs/bitstream/id/2743/A_Single-Cell_Raman_Spectroscopy_Analysis_pub_2022.pdf | |
dc.type.version | publishedVersion | |