A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
Аутори
Kukolj, TamaraLazarević, Jasmina
Borojević, Ana
Ralević, Uroš
Vujić, Dragana
Jauković, Aleksandra
Lazarević, Nenad
Bugarski, Diana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After exten...sive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.
Кључне речи:
human bone marrow mesenchymal stem/stromal cells (BM-MSCs) / Raman spectroscopy / single cell / inter-individual heterogeneityИзвор:
International Journal of Molecular Sciences, 2022, 23, 9, 4915-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200015 (Универзитет у Београду, Институт за медицинска истраживања) (RS-200015)
- "Start up for science” 2019/2020 - Center for Leadership Development and the Philip Morris Company in Serbia
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200024 (Универзитет у Београду, Институт за физику, Београд-Земун) (RS-200024)
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Kukolj, Tamara AU - Lazarević, Jasmina AU - Borojević, Ana AU - Ralević, Uroš AU - Vujić, Dragana AU - Jauković, Aleksandra AU - Lazarević, Nenad AU - Bugarski, Diana PY - 2022 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1235 AB - The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches. PB - MDPI T2 - International Journal of Molecular Sciences T1 - A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity IS - 9 SP - 4915 VL - 23 DO - 10.3390/ijms23094915 ER -
@article{ author = "Kukolj, Tamara and Lazarević, Jasmina and Borojević, Ana and Ralević, Uroš and Vujić, Dragana and Jauković, Aleksandra and Lazarević, Nenad and Bugarski, Diana", year = "2022", abstract = "The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.", publisher = "MDPI", journal = "International Journal of Molecular Sciences", title = "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity", number = "9", pages = "4915", volume = "23", doi = "10.3390/ijms23094915" }
Kukolj, T., Lazarević, J., Borojević, A., Ralević, U., Vujić, D., Jauković, A., Lazarević, N.,& Bugarski, D.. (2022). A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences MDPI., 23(9), 4915. https://doi.org/10.3390/ijms23094915
Kukolj T, Lazarević J, Borojević A, Ralević U, Vujić D, Jauković A, Lazarević N, Bugarski D. A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences. 2022;23(9):4915. doi:10.3390/ijms23094915 .
Kukolj, Tamara, Lazarević, Jasmina, Borojević, Ana, Ralević, Uroš, Vujić, Dragana, Jauković, Aleksandra, Lazarević, Nenad, Bugarski, Diana, "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity" in International Journal of Molecular Sciences, 23, no. 9 (2022):4915, https://doi.org/10.3390/ijms23094915 . .