TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Massaro, Marika
AU  - Carluccio, Maria Annunziata
AU  - Arola-Arnal, Anna
AU  - Muguerza, Begona
AU  - Vanden Berghe, Wim
AU  - Declerk, Ken
AU  - Isabel Bravo, Francisca
AU  - Calabriso, Nadia
AU  - Combet, Emilie
AU  - Gibney, Eileen R.
AU  - Gomes, Andreia
AU  - Gonthier, Marie-Paule
AU  - Kistanova, Elena
AU  - Krga, Irena
AU  - Mena, Pedro
AU  - Morand, Christine
AU  - dos Santos, Claudia Nunes
AU  - De Pascual-Teresa, Sonia
AU  - Rodriguez-Mateos, Ana
AU  - Scoditti, Egeria
AU  - Suarez, Manuel
AU  - Milenković, Dragan
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1058
AB  - Flavanol intake positively influences several cardiometabolic risk factors in humans. However, the specific molecular mechanisms of action of flavanols, in terms of gene regulation, in the cell types relevant to cardiometabolic disease have never been systematically addressed. On this basis, we conducted a systematic literature review and a comprehensive bioinformatic analysis of genes whose expression is affected by flavanols in cells defining cardiometabolic health: hepatocytes, adipocytes, endothelial cells, smooth muscle cells and immune cells. A systematic literature search was performed using the following pre-defined criteria: treatment with pure compounds and metabolites (no extracts) at low concentrations that are close to their plasma concentrations. Differentially expressed genes were analyzed using bioinformatics tools to identify gene ontologies, networks, cellular pathways and interactions, as well as transcriptional and post-transcriptional regulators. The systematic literature search identified 54 differentially expressed genes at the mRNA level inin vitromodels of cardiometabolic disease exposed to flavanols and their metabolites. Global bioinformatic analysis revealed that these genes are predominantly involved in inflammation, leukocyte adhesion and transendothelial migration, and lipid metabolism. We observed that, although the investigated cells responded differentially to flavanol exposure, the involvement of anti-inflammatory responses is a common mechanism of flavanol action. We also identified potential transcriptional regulators of gene expression: transcriptional factors, such as GATA2, NFKB1, FOXC1 or PPARG, and post-transcriptional regulators: miRNAs, such as mir-335-5p, let-7b-5p, mir-26b-5p or mir-16-5p. In parallel, we analyzed the nutrigenomic effects of flavanols in intestinal cells and demonstrated their predominant involvement in the metabolism of circulating lipoproteins. In conclusion, the results of this systematic analysis of the nutrigenomic effects of flavanols provide a more comprehensive picture of their molecular mechanisms of action and will support the future setup of genetic studies to pave the way for individualized dietary recommendations.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease
EP  - 5064
IS  - 6
SP  - 5040
VL  - 11
DO  - 10.1039/d0fo00701c
ER  - 

@article{
author = "Ruskovska, Tatjana and Massaro, Marika and Carluccio, Maria Annunziata and Arola-Arnal, Anna and Muguerza, Begona and Vanden Berghe, Wim and Declerk, Ken and Isabel Bravo, Francisca and Calabriso, Nadia and Combet, Emilie and Gibney, Eileen R. and Gomes, Andreia and Gonthier, Marie-Paule and Kistanova, Elena and Krga, Irena and Mena, Pedro and Morand, Christine and dos Santos, Claudia Nunes and De Pascual-Teresa, Sonia and Rodriguez-Mateos, Ana and Scoditti, Egeria and Suarez, Manuel and Milenković, Dragan",
year = "2020",
abstract = "Flavanol intake positively influences several cardiometabolic risk factors in humans. However, the specific molecular mechanisms of action of flavanols, in terms of gene regulation, in the cell types relevant to cardiometabolic disease have never been systematically addressed. On this basis, we conducted a systematic literature review and a comprehensive bioinformatic analysis of genes whose expression is affected by flavanols in cells defining cardiometabolic health: hepatocytes, adipocytes, endothelial cells, smooth muscle cells and immune cells. A systematic literature search was performed using the following pre-defined criteria: treatment with pure compounds and metabolites (no extracts) at low concentrations that are close to their plasma concentrations. Differentially expressed genes were analyzed using bioinformatics tools to identify gene ontologies, networks, cellular pathways and interactions, as well as transcriptional and post-transcriptional regulators. The systematic literature search identified 54 differentially expressed genes at the mRNA level inin vitromodels of cardiometabolic disease exposed to flavanols and their metabolites. Global bioinformatic analysis revealed that these genes are predominantly involved in inflammation, leukocyte adhesion and transendothelial migration, and lipid metabolism. We observed that, although the investigated cells responded differentially to flavanol exposure, the involvement of anti-inflammatory responses is a common mechanism of flavanol action. We also identified potential transcriptional regulators of gene expression: transcriptional factors, such as GATA2, NFKB1, FOXC1 or PPARG, and post-transcriptional regulators: miRNAs, such as mir-335-5p, let-7b-5p, mir-26b-5p or mir-16-5p. In parallel, we analyzed the nutrigenomic effects of flavanols in intestinal cells and demonstrated their predominant involvement in the metabolism of circulating lipoproteins. In conclusion, the results of this systematic analysis of the nutrigenomic effects of flavanols provide a more comprehensive picture of their molecular mechanisms of action and will support the future setup of genetic studies to pave the way for individualized dietary recommendations.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease",
pages = "5064-5040",
number = "6",
volume = "11",
doi = "10.1039/d0fo00701c"
}

Ruskovska, T., Massaro, M., Carluccio, M. A., Arola-Arnal, A., Muguerza, B., Vanden Berghe, W., Declerk, K., Isabel Bravo, F., Calabriso, N., Combet, E., Gibney, E. R., Gomes, A., Gonthier, M., Kistanova, E., Krga, I., Mena, P., Morand, C., dos Santos, C. N., De Pascual-Teresa, S., Rodriguez-Mateos, A., Scoditti, E., Suarez, M.,& Milenković, D.. (2020). Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease. in Food & Function
Royal Soc Chemistry, Cambridge., 11(6), 5040-5064.
https://doi.org/10.1039/d0fo00701c

Ruskovska T, Massaro M, Carluccio MA, Arola-Arnal A, Muguerza B, Vanden Berghe W, Declerk K, Isabel Bravo F, Calabriso N, Combet E, Gibney ER, Gomes A, Gonthier M, Kistanova E, Krga I, Mena P, Morand C, dos Santos CN, De Pascual-Teresa S, Rodriguez-Mateos A, Scoditti E, Suarez M, Milenković D. Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease. in Food & Function. 2020;11(6):5040-5064.
doi:10.1039/d0fo00701c .

Ruskovska, Tatjana, Massaro, Marika, Carluccio, Maria Annunziata, Arola-Arnal, Anna, Muguerza, Begona, Vanden Berghe, Wim, Declerk, Ken, Isabel Bravo, Francisca, Calabriso, Nadia, Combet, Emilie, Gibney, Eileen R., Gomes, Andreia, Gonthier, Marie-Paule, Kistanova, Elena, Krga, Irena, Mena, Pedro, Morand, Christine, dos Santos, Claudia Nunes, De Pascual-Teresa, Sonia, Rodriguez-Mateos, Ana, Scoditti, Egeria, Suarez, Manuel, Milenković, Dragan, "Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease" in Food & Function, 11, no. 6 (2020):5040-5064,
https://doi.org/10.1039/d0fo00701c . .

TY  - JOUR
AU  - Pokimica, Biljana
AU  - Garcia-Conesa, Maria-Teresa
AU  - Zec, Manja M.
AU  - Debeljak-Martačić, Jasmina
AU  - Ranković, Slavica G.
AU  - Vidović, Nevena Đ.
AU  - Petrović-Oggiano, Gordana P.
AU  - Konić-Ristić, Aleksandra
AU  - Glibetić, Marija
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/977
AB  - Chokeberry polyphenols have been suggested to reduce cholesterol and blood pressure and thus protect against cardiovascular diseases (CVD), but the evidence in humans is limited and inconsistent. This randomized double-blinded three-parallel groups trial investigated the changes in various anthropometric and clinical biomarkers, and in plasma phospholipids fatty acids (PPFA) in volunteers at cardiovascular risk after a four-week intervention with 100 mL/day of (1) chokeberry juice with a high-dose of polyphenols (1177.11 mg gallic acid equivalents, GAE); (2) chokeberry juice with a low-dose of polyphenols (294.28 mg GAE) and; (3) a nutritionally matched polyphenol-free placebo drink. Our results indicate that the intake of chokeberry juice containing either the low or the high dose of polyphenols cannot be linked with a reduction in total- and low-density lipoprotein (LDL)cholesterol or in systolic (SBP) and diastolic (DBP) blood pressure in comparison with the consumption of the placebo drink. However, we found evidence of moderate changes in the PPFA, i.e., increased saturated fatty acids (SFA), mostly palmitic acid, and reduced n-6 polyunsaturated fatty acids (PUFA), principally linoleic acid (LA) with the intake of chokeberry against the placebo. These effects may be associated with the polyphenols but we could not differentiate a clear dose-response effect. Further research is still needed to elucidate the contribution of the polyphenolic fraction to the potential cardiovascular effects of the chokeberry and to build up the evidence of its potential benefit via the modulation of PPFA composition.
PB  - MDPI, Basel
T2  - Nutrients
T1  - Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk
IS  - 4
VL  - 11
DO  - 10.3390/nu11040850
ER  - 

@article{
author = "Pokimica, Biljana and Garcia-Conesa, Maria-Teresa and Zec, Manja M. and Debeljak-Martačić, Jasmina and Ranković, Slavica G. and Vidović, Nevena Đ. and Petrović-Oggiano, Gordana P. and Konić-Ristić, Aleksandra and Glibetić, Marija",
year = "2019",
abstract = "Chokeberry polyphenols have been suggested to reduce cholesterol and blood pressure and thus protect against cardiovascular diseases (CVD), but the evidence in humans is limited and inconsistent. This randomized double-blinded three-parallel groups trial investigated the changes in various anthropometric and clinical biomarkers, and in plasma phospholipids fatty acids (PPFA) in volunteers at cardiovascular risk after a four-week intervention with 100 mL/day of (1) chokeberry juice with a high-dose of polyphenols (1177.11 mg gallic acid equivalents, GAE); (2) chokeberry juice with a low-dose of polyphenols (294.28 mg GAE) and; (3) a nutritionally matched polyphenol-free placebo drink. Our results indicate that the intake of chokeberry juice containing either the low or the high dose of polyphenols cannot be linked with a reduction in total- and low-density lipoprotein (LDL)cholesterol or in systolic (SBP) and diastolic (DBP) blood pressure in comparison with the consumption of the placebo drink. However, we found evidence of moderate changes in the PPFA, i.e., increased saturated fatty acids (SFA), mostly palmitic acid, and reduced n-6 polyunsaturated fatty acids (PUFA), principally linoleic acid (LA) with the intake of chokeberry against the placebo. These effects may be associated with the polyphenols but we could not differentiate a clear dose-response effect. Further research is still needed to elucidate the contribution of the polyphenolic fraction to the potential cardiovascular effects of the chokeberry and to build up the evidence of its potential benefit via the modulation of PPFA composition.",
publisher = "MDPI, Basel",
journal = "Nutrients",
title = "Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk",
number = "4",
volume = "11",
doi = "10.3390/nu11040850"
}

Pokimica, B., Garcia-Conesa, M., Zec, M. M., Debeljak-Martačić, J., Ranković, S. G., Vidović, N. Đ., Petrović-Oggiano, G. P., Konić-Ristić, A.,& Glibetić, M.. (2019). Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk. in Nutrients
MDPI, Basel., 11(4).
https://doi.org/10.3390/nu11040850

Pokimica B, Garcia-Conesa M, Zec MM, Debeljak-Martačić J, Ranković SG, Vidović NĐ, Petrović-Oggiano GP, Konić-Ristić A, Glibetić M. Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk. in Nutrients. 2019;11(4).
doi:10.3390/nu11040850 .

Pokimica, Biljana, Garcia-Conesa, Maria-Teresa, Zec, Manja M., Debeljak-Martačić, Jasmina, Ranković, Slavica G., Vidović, Nevena Đ., Petrović-Oggiano, Gordana P., Konić-Ristić, Aleksandra, Glibetić, Marija, "Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk" in Nutrients, 11, no. 4 (2019),
https://doi.org/10.3390/nu11040850 . .

TY  - JOUR
AU  - Nikolić, Marina
AU  - Konić-Ristić, Aleksandra
AU  - Gonzalez-Sarrias, Antonio
AU  - Istas, Geoffrey
AU  - Urpi-Sarda, Mireia
AU  - Dall'Asta, Margherita
AU  - Monfoulet, Laurent-Emanuel
AU  - Cloetens, Lieselotte
AU  - Bayram, Banu
AU  - Tumolo, Maria Rosaria
AU  - Chervenkov, Mihail
AU  - Scoditti, Egeria
AU  - Massaro, Marika
AU  - Tejera, Noemi
AU  - Abadjieva, Desislava
AU  - Chambers, Karen
AU  - Krga, Irena
AU  - Tomas-Barberan, Francisco A.
AU  - Morand, Christine
AU  - Feliciano, Rodrigo
AU  - Garcia-Villalba, Rocio
AU  - Garcia-Aloy, Mar
AU  - Mena, Pedro
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/933
AB  - Key messages The reporting quality of human studies on inter-individual variation in response to plant bioactives is generally low and should be significantly improved. There is no specific guidance for reporting studies on inter-individual variation in response to plant bioactives. The assessment of reporting quality using a scale approach is considered a valuable tool in assessing compliance with the recommendations in the submission phase or during the reviewing process. It also provides a quantitative measure of the quality of studies to be used in meta-analysis. Eleven reporting criteria were developed and supported by detailed definitions and guidance for their scoring. The POSITIVe quality index was tested and demonstrated to be valid, reliable, and responsive. The use of the quality index and its supporting explanatory material (dictionary) as a guide for researchers, peer-reviewers, and journal editors will foster further complete and transparent reporting of data on inter-individual variability. The criteria used in the quality index can serve as additional guidance to inform the design and conduction of further studies on inter-individual variations in response to plant bioactives. Better reporting is expected to lead to a better understanding of the mechanisms and factors involved and thus better study designs with greater impact on policies and practice. Purpose The quality of the study design and data reporting in human trials dealing with the inter-individual variability in response to the consumption of plant bioactives is, in general, low. There is a lack of recommendations supporting the scientific community on this topic. This study aimed at developing a quality index to assist the assessment of the reporting quality of intervention trials addressing the inter-individual variability in response to plant bioactive consumption. Recommendations for better designing and reporting studies were discussed. Methods The selection of the parameters used for the development of the quality index was carried out in agreement with the scientific community through a survey. Parameters were defined, grouped into categories, and scored for different quality levels. The applicability of the scoring system was tested in terms of consistency and effort, and its validity was assessed by comparison with a simultaneous evaluation by experts' criteria. Results The "POSITIVe quality index" included 11 reporting criteria grouped into four categories (Statistics, Reporting, Data presentation, and Individual data availability). It was supported by detailed definitions and guidance for their scoring. The quality index score was tested, and the index demonstrated to be valid, reliable, and responsive. Conclusions The evaluation of the reporting quality of studies addressing inter-individual variability in response to plant bioactives highlighted the aspects requiring major improvements. Specific tools and recommendations favoring a complete and transparent reporting on inter-individual variability have been provided to support the scientific community on this field.
PB  - Springer Heidelberg, Heidelberg
T2  - European Journal of Nutrition
T1  - Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations
EP  - S64
SP  - S49
VL  - 58
DO  - 10.1007/s00394-019-02069-3
ER  - 

@article{
author = "Nikolić, Marina and Konić-Ristić, Aleksandra and Gonzalez-Sarrias, Antonio and Istas, Geoffrey and Urpi-Sarda, Mireia and Dall'Asta, Margherita and Monfoulet, Laurent-Emanuel and Cloetens, Lieselotte and Bayram, Banu and Tumolo, Maria Rosaria and Chervenkov, Mihail and Scoditti, Egeria and Massaro, Marika and Tejera, Noemi and Abadjieva, Desislava and Chambers, Karen and Krga, Irena and Tomas-Barberan, Francisco A. and Morand, Christine and Feliciano, Rodrigo and Garcia-Villalba, Rocio and Garcia-Aloy, Mar and Mena, Pedro",
year = "2019",
abstract = "Key messages The reporting quality of human studies on inter-individual variation in response to plant bioactives is generally low and should be significantly improved. There is no specific guidance for reporting studies on inter-individual variation in response to plant bioactives. The assessment of reporting quality using a scale approach is considered a valuable tool in assessing compliance with the recommendations in the submission phase or during the reviewing process. It also provides a quantitative measure of the quality of studies to be used in meta-analysis. Eleven reporting criteria were developed and supported by detailed definitions and guidance for their scoring. The POSITIVe quality index was tested and demonstrated to be valid, reliable, and responsive. The use of the quality index and its supporting explanatory material (dictionary) as a guide for researchers, peer-reviewers, and journal editors will foster further complete and transparent reporting of data on inter-individual variability. The criteria used in the quality index can serve as additional guidance to inform the design and conduction of further studies on inter-individual variations in response to plant bioactives. Better reporting is expected to lead to a better understanding of the mechanisms and factors involved and thus better study designs with greater impact on policies and practice. Purpose The quality of the study design and data reporting in human trials dealing with the inter-individual variability in response to the consumption of plant bioactives is, in general, low. There is a lack of recommendations supporting the scientific community on this topic. This study aimed at developing a quality index to assist the assessment of the reporting quality of intervention trials addressing the inter-individual variability in response to plant bioactive consumption. Recommendations for better designing and reporting studies were discussed. Methods The selection of the parameters used for the development of the quality index was carried out in agreement with the scientific community through a survey. Parameters were defined, grouped into categories, and scored for different quality levels. The applicability of the scoring system was tested in terms of consistency and effort, and its validity was assessed by comparison with a simultaneous evaluation by experts' criteria. Results The "POSITIVe quality index" included 11 reporting criteria grouped into four categories (Statistics, Reporting, Data presentation, and Individual data availability). It was supported by detailed definitions and guidance for their scoring. The quality index score was tested, and the index demonstrated to be valid, reliable, and responsive. Conclusions The evaluation of the reporting quality of studies addressing inter-individual variability in response to plant bioactives highlighted the aspects requiring major improvements. Specific tools and recommendations favoring a complete and transparent reporting on inter-individual variability have been provided to support the scientific community on this field.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "European Journal of Nutrition",
title = "Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations",
pages = "S64-S49",
volume = "58",
doi = "10.1007/s00394-019-02069-3"
}

Nikolić, M., Konić-Ristić, A., Gonzalez-Sarrias, A., Istas, G., Urpi-Sarda, M., Dall'Asta, M., Monfoulet, L., Cloetens, L., Bayram, B., Tumolo, M. R., Chervenkov, M., Scoditti, E., Massaro, M., Tejera, N., Abadjieva, D., Chambers, K., Krga, I., Tomas-Barberan, F. A., Morand, C., Feliciano, R., Garcia-Villalba, R., Garcia-Aloy, M.,& Mena, P.. (2019). Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations. in European Journal of Nutrition
Springer Heidelberg, Heidelberg., 58, S49-S64.
https://doi.org/10.1007/s00394-019-02069-3

Nikolić M, Konić-Ristić A, Gonzalez-Sarrias A, Istas G, Urpi-Sarda M, Dall'Asta M, Monfoulet L, Cloetens L, Bayram B, Tumolo MR, Chervenkov M, Scoditti E, Massaro M, Tejera N, Abadjieva D, Chambers K, Krga I, Tomas-Barberan FA, Morand C, Feliciano R, Garcia-Villalba R, Garcia-Aloy M, Mena P. Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations. in European Journal of Nutrition. 2019;58:S49-S64.
doi:10.1007/s00394-019-02069-3 .

Nikolić, Marina, Konić-Ristić, Aleksandra, Gonzalez-Sarrias, Antonio, Istas, Geoffrey, Urpi-Sarda, Mireia, Dall'Asta, Margherita, Monfoulet, Laurent-Emanuel, Cloetens, Lieselotte, Bayram, Banu, Tumolo, Maria Rosaria, Chervenkov, Mihail, Scoditti, Egeria, Massaro, Marika, Tejera, Noemi, Abadjieva, Desislava, Chambers, Karen, Krga, Irena, Tomas-Barberan, Francisco A., Morand, Christine, Feliciano, Rodrigo, Garcia-Villalba, Rocio, Garcia-Aloy, Mar, Mena, Pedro, "Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations" in European Journal of Nutrition, 58 (2019):S49-S64,
https://doi.org/10.1007/s00394-019-02069-3 . .

TY  - JOUR
AU  - Pokimica, Biljana
AU  - Garcia-Conesa, Maria-Teresa
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/861
AB  - Pre-clinical cell and animal nutrigenomic studies have long suggested the modulation of the transcription of multiple gene targets in cells and tissues as a potential molecular mechanism of action underlying the beneficial effects attributed to plant-derived bioactive compounds. To try to demonstrate these molecular effects in humans, a considerable number of clinical trials have now explored the changes in the expression levels of selected genes in various human cell and tissue samples following intervention with different dietary sources of bioactive compounds. In this review, we have compiled a total of 75 human studies exploring gene expression changes using quantitative reverse transcription PCR (RT-qPCR). We have critically appraised the study design and methodology used as well as the gene expression results reported. We herein pinpoint some of the main drawbacks and gaps in the experimental strategies applied, as well as the high interindividual variability of the results and the limited evidence supporting some of the investigated genes as potential responsive targets. We reinforce the need to apply normalized procedures and follow well-established methodological guidelines in future studies in order to achieve improved and reliable results that would allow for more relevant and biologically meaningful results.
PB  - MDPI, Basel
T2  - Nutrients
T1  - Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies
IS  - 7
VL  - 10
DO  - 10.3390/nu10070807
ER  - 

@article{
author = "Pokimica, Biljana and Garcia-Conesa, Maria-Teresa",
year = "2018",
abstract = "Pre-clinical cell and animal nutrigenomic studies have long suggested the modulation of the transcription of multiple gene targets in cells and tissues as a potential molecular mechanism of action underlying the beneficial effects attributed to plant-derived bioactive compounds. To try to demonstrate these molecular effects in humans, a considerable number of clinical trials have now explored the changes in the expression levels of selected genes in various human cell and tissue samples following intervention with different dietary sources of bioactive compounds. In this review, we have compiled a total of 75 human studies exploring gene expression changes using quantitative reverse transcription PCR (RT-qPCR). We have critically appraised the study design and methodology used as well as the gene expression results reported. We herein pinpoint some of the main drawbacks and gaps in the experimental strategies applied, as well as the high interindividual variability of the results and the limited evidence supporting some of the investigated genes as potential responsive targets. We reinforce the need to apply normalized procedures and follow well-established methodological guidelines in future studies in order to achieve improved and reliable results that would allow for more relevant and biologically meaningful results.",
publisher = "MDPI, Basel",
journal = "Nutrients",
title = "Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies",
number = "7",
volume = "10",
doi = "10.3390/nu10070807"
}

Pokimica, B.,& Garcia-Conesa, M.. (2018). Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies. in Nutrients
MDPI, Basel., 10(7).
https://doi.org/10.3390/nu10070807

Pokimica B, Garcia-Conesa M. Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies. in Nutrients. 2018;10(7).
doi:10.3390/nu10070807 .

Pokimica, Biljana, Garcia-Conesa, Maria-Teresa, "Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies" in Nutrients, 10, no. 7 (2018),
https://doi.org/10.3390/nu10070807 . .

TY  - JOUR
AU  - Krga, Irena
AU  - Milenković, Dragan
AU  - Morand, Christine
AU  - Monfoulet, Laurent-Emanuel
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/702
AB  - Polyphenols are plant food microconstituents that are widely distributed in the human diet, with fruits and fruit-derived products as one of the main dietary sources. Epidemiological studies have shown an inverse relationship between the intake of different classes of polyphenols and the risk of myocardial infarction or cardiovascular disease (CVD) mortality. These compounds have been associated with the promotion of cardiovascular health as evidenced by clinical studies reporting beneficial effects of polyphenol-rich fruit consumption on intermediate markers of cardiovascular diseases. Additionally, animal and in vitro studies have indicated positive roles of polyphenols in preventing dysfunctions associated with the development of cardiovascular diseases. However, the mechanisms of action underlying their beneficial effects appear complex and are not fully understood. This review aims to provide an update on the nutrigenomic effects of different groups of polyphenols from fruits and especially focuses on their cardiovascular protective effects in cell and animal studies.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols
EP  - 3676
IS  - 9
SP  - 3656
VL  - 7
DO  - 10.1039/c6fo00596a
ER  - 

@article{
author = "Krga, Irena and Milenković, Dragan and Morand, Christine and Monfoulet, Laurent-Emanuel",
year = "2016",
abstract = "Polyphenols are plant food microconstituents that are widely distributed in the human diet, with fruits and fruit-derived products as one of the main dietary sources. Epidemiological studies have shown an inverse relationship between the intake of different classes of polyphenols and the risk of myocardial infarction or cardiovascular disease (CVD) mortality. These compounds have been associated with the promotion of cardiovascular health as evidenced by clinical studies reporting beneficial effects of polyphenol-rich fruit consumption on intermediate markers of cardiovascular diseases. Additionally, animal and in vitro studies have indicated positive roles of polyphenols in preventing dysfunctions associated with the development of cardiovascular diseases. However, the mechanisms of action underlying their beneficial effects appear complex and are not fully understood. This review aims to provide an update on the nutrigenomic effects of different groups of polyphenols from fruits and especially focuses on their cardiovascular protective effects in cell and animal studies.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols",
pages = "3676-3656",
number = "9",
volume = "7",
doi = "10.1039/c6fo00596a"
}

Krga, I., Milenković, D., Morand, C.,& Monfoulet, L.. (2016). An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols. in Food & Function
Royal Soc Chemistry, Cambridge., 7(9), 3656-3676.
https://doi.org/10.1039/c6fo00596a

Krga I, Milenković D, Morand C, Monfoulet L. An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols. in Food & Function. 2016;7(9):3656-3676.
doi:10.1039/c6fo00596a .

Krga, Irena, Milenković, Dragan, Morand, Christine, Monfoulet, Laurent-Emanuel, "An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols" in Food & Function, 7, no. 9 (2016):3656-3676,
https://doi.org/10.1039/c6fo00596a . .