TY  - JOUR
AU  - Krga, Irena
AU  - Ruskovska, Tatjana
AU  - Milenković, Dragan
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1294
PB  - Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Editorial: Plant food bioactives, genomics, and health effects
SP  - 1166149
VL  - 10
DO  - 10.3389/fnut.2023.1166149
ER  - 

@article{
author = "Krga, Irena and Ruskovska, Tatjana and Milenković, Dragan",
year = "2023",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Editorial: Plant food bioactives, genomics, and health effects",
pages = "1166149",
volume = "10",
doi = "10.3389/fnut.2023.1166149"
}

Krga, I., Ruskovska, T.,& Milenković, D.. (2023). Editorial: Plant food bioactives, genomics, and health effects. in Frontiers in Nutrition
Frontiers Media S.A.., 10, 1166149.
https://doi.org/10.3389/fnut.2023.1166149

Krga I, Ruskovska T, Milenković D. Editorial: Plant food bioactives, genomics, and health effects. in Frontiers in Nutrition. 2023;10:1166149.
doi:10.3389/fnut.2023.1166149 .

Krga, Irena, Ruskovska, Tatjana, Milenković, Dragan, "Editorial: Plant food bioactives, genomics, and health effects" in Frontiers in Nutrition, 10 (2023):1166149,
https://doi.org/10.3389/fnut.2023.1166149 . .

TY  - JOUR
AU  - Bochenek, Heidi
AU  - Krga, Irena
AU  - Sergi, Domenico
AU  - Kouvari, Matina
AU  - Zec, Manja
AU  - Naumovski, Nenad
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1203
AB  - Cardiovascular disease (CVD) and cancers are overall still identified as the two most prevalent non-communicable diseases globally. Their prevention and potential reversal (in particular CVD risk) was seen effective with the modification of dietary intake that was applied in several different populations. Although the findings from epidemiological studies provide support that adhering to dietary patterns such as the Mediterranean diet can reduce incidence and prevalence of CVD and some forms of cancer, the mechanistic aspects of disease modulation associated with both diseases can be seen in dietary management. Several studies have already explored the potential modes of action of certain nutrients in well controlled large clinical trials. However, the clinical trials designed to determine the effects of adhering to a particular diet are relatively hard to conduct and these studies are faced with several obstacles particularly in the populations that are identified with a high risk of CVD or different cancers. Therefore, it is important to understand potential underlying and shared mechanisms of action and to explore how healthy dietary patterns may modulate the occurrence, initiation, and progression of such diseases. The aim of this review is to summarise and conceptualize the current understanding relating to healthy dietary patterns, and briefly discuss the opportunities that epigenetic research may bring and how it may assist to further interpret epidemiological and clinical evidence.
PB  - IMR Press Limited
T2  - Reviews in Cariovascular Medicine
T1  - Dietary patterns, caloric restrictions for management of cardiovascular disease and cancer; a brief review
IS  - 1
SP  - 041
VL  - 23
DO  - 10.31083/j.rcm2301041
ER  - 

@article{
author = "Bochenek, Heidi and Krga, Irena and Sergi, Domenico and Kouvari, Matina and Zec, Manja and Naumovski, Nenad",
year = "2022",
abstract = "Cardiovascular disease (CVD) and cancers are overall still identified as the two most prevalent non-communicable diseases globally. Their prevention and potential reversal (in particular CVD risk) was seen effective with the modification of dietary intake that was applied in several different populations. Although the findings from epidemiological studies provide support that adhering to dietary patterns such as the Mediterranean diet can reduce incidence and prevalence of CVD and some forms of cancer, the mechanistic aspects of disease modulation associated with both diseases can be seen in dietary management. Several studies have already explored the potential modes of action of certain nutrients in well controlled large clinical trials. However, the clinical trials designed to determine the effects of adhering to a particular diet are relatively hard to conduct and these studies are faced with several obstacles particularly in the populations that are identified with a high risk of CVD or different cancers. Therefore, it is important to understand potential underlying and shared mechanisms of action and to explore how healthy dietary patterns may modulate the occurrence, initiation, and progression of such diseases. The aim of this review is to summarise and conceptualize the current understanding relating to healthy dietary patterns, and briefly discuss the opportunities that epigenetic research may bring and how it may assist to further interpret epidemiological and clinical evidence.",
publisher = "IMR Press Limited",
journal = "Reviews in Cariovascular Medicine",
title = "Dietary patterns, caloric restrictions for management of cardiovascular disease and cancer; a brief review",
number = "1",
pages = "041",
volume = "23",
doi = "10.31083/j.rcm2301041"
}

Bochenek, H., Krga, I., Sergi, D., Kouvari, M., Zec, M.,& Naumovski, N.. (2022). Dietary patterns, caloric restrictions for management of cardiovascular disease and cancer; a brief review. in Reviews in Cariovascular Medicine
IMR Press Limited., 23(1), 041.
https://doi.org/10.31083/j.rcm2301041

Bochenek H, Krga I, Sergi D, Kouvari M, Zec M, Naumovski N. Dietary patterns, caloric restrictions for management of cardiovascular disease and cancer; a brief review. in Reviews in Cariovascular Medicine. 2022;23(1):041.
doi:10.31083/j.rcm2301041 .

Bochenek, Heidi, Krga, Irena, Sergi, Domenico, Kouvari, Matina, Zec, Manja, Naumovski, Nenad, "Dietary patterns, caloric restrictions for management of cardiovascular disease and cancer; a brief review" in Reviews in Cariovascular Medicine, 23, no. 1 (2022):041,
https://doi.org/10.31083/j.rcm2301041 . .

TY  - JOUR
AU  - Krga, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Barber-Chamoux, Nicolas
AU  - Dubray, Claude
AU  - Morand, Christine
AU  - Milenković, Dragan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1243
AB  - Grapefruit is a rich source of flavanones, phytochemicals suggested excreting
vasculoprotective effects. We previously showed that flavanones in grapefruit juice
(GFJ) reduced postmenopausal women’s pulse-wave velocity (PWV), a measure of
arterial stiffness. However, mechanisms of flavanone action in humans are largely
unknown. This study aimed to decipher molecular mechanisms of flavanones by
multi-omics analysis in PBMCs of volunteers consuming GFJ and flavanone-free
control drink for 6 months. Modulated genes and microRNAs (miRNAs) were identified
using microarrays. Bioinformatics analyses assessed their functions, interactions and
correlations with previously observed changes in PWV. GFJ modified gene and
miRNA expressions. Integrated analysis of modulated genes and miRNA-target genes
suggests regulation of inflammation, immune response, cell interaction and mobility.
Bioinformatics identified putative mediators of the observed nutrigenomic effect
(STAT3, NF-κB) and molecular docking demonstrated potential binding of flavanone
metabolites to transcription factors and cell-signaling proteins. We also observed 34
significant correlations between changes in gene expression and PWV. Moreover, global
gene expression was negatively correlated with gene expression profiles in arterial
stiffness and hypertension. This study revealed molecular mechanisms underlying
vasculoprotective effects of flavanones, including interactions with transcription factors
and gene and miRNA expression changes that inversely correlate with gene expression
profiles associated with cardiovascular risk factors.
PB  - Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women
SP  - 907595
VL  - 9
VL  - 9
DO  - 10.3389/fnut.2022.907595
ER  - 

@article{
author = "Krga, Irena and Corral-Jara, Karla Fabiola and Barber-Chamoux, Nicolas and Dubray, Claude and Morand, Christine and Milenković, Dragan",
year = "2022",
abstract = "Grapefruit is a rich source of flavanones, phytochemicals suggested excreting
vasculoprotective effects. We previously showed that flavanones in grapefruit juice
(GFJ) reduced postmenopausal women’s pulse-wave velocity (PWV), a measure of
arterial stiffness. However, mechanisms of flavanone action in humans are largely
unknown. This study aimed to decipher molecular mechanisms of flavanones by
multi-omics analysis in PBMCs of volunteers consuming GFJ and flavanone-free
control drink for 6 months. Modulated genes and microRNAs (miRNAs) were identified
using microarrays. Bioinformatics analyses assessed their functions, interactions and
correlations with previously observed changes in PWV. GFJ modified gene and
miRNA expressions. Integrated analysis of modulated genes and miRNA-target genes
suggests regulation of inflammation, immune response, cell interaction and mobility.
Bioinformatics identified putative mediators of the observed nutrigenomic effect
(STAT3, NF-κB) and molecular docking demonstrated potential binding of flavanone
metabolites to transcription factors and cell-signaling proteins. We also observed 34
significant correlations between changes in gene expression and PWV. Moreover, global
gene expression was negatively correlated with gene expression profiles in arterial
stiffness and hypertension. This study revealed molecular mechanisms underlying
vasculoprotective effects of flavanones, including interactions with transcription factors
and gene and miRNA expression changes that inversely correlate with gene expression
profiles associated with cardiovascular risk factors.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women",
pages = "907595",
volume = "9, 9",
doi = "10.3389/fnut.2022.907595"
}

Krga, I., Corral-Jara, K. F., Barber-Chamoux, N., Dubray, C., Morand, C.,& Milenković, D.. (2022). Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women. in Frontiers in Nutrition
Frontiers Media S.A.., 9, 907595.
https://doi.org/10.3389/fnut.2022.907595

Krga I, Corral-Jara KF, Barber-Chamoux N, Dubray C, Morand C, Milenković D. Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women. in Frontiers in Nutrition. 2022;9:907595.
doi:10.3389/fnut.2022.907595 .

Krga, Irena, Corral-Jara, Karla Fabiola, Barber-Chamoux, Nicolas, Dubray, Claude, Morand, Christine, Milenković, Dragan, "Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women" in Frontiers in Nutrition, 9 (2022):907595,
https://doi.org/10.3389/fnut.2022.907595 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Budić-Leto, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Ajdžanović, Vladimir
AU  - Arola-Arnal, Anna
AU  - Bravo, Francisca Isabel
AU  - Deligiannidou, Georgia Erini
AU  - Havlik, Jaroslav
AU  - Janeva, Milkica
AU  - Kistanova, Elena
AU  - Kontogiorgis, Christos
AU  - Krga, Irena
AU  - Massaro, Marika
AU  - Miler, Marko
AU  - Harnafi, Hicham
AU  - Milosevic, Verica
AU  - Morand, Christine
AU  - Scoditti, Egeria
AU  - Suárez, Manuel
AU  - Vauzour, David
AU  - Milenkovic, Dragan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1234
AB  - Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.
PB  - Elsevier
T2  - Ageing Research Reviews
T2  - Ageing Research Reviews
T1  - Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans – Evidence from untargeted mRNA and miRNA studies
IS  - 101649
VL  - 79
DO  - 10.1016/j.arr.2022.101649
ER  - 

@article{
author = "Ruskovska, Tatjana and Budić-Leto, Irena and Corral-Jara, Karla Fabiola and Ajdžanović, Vladimir and Arola-Arnal, Anna and Bravo, Francisca Isabel and Deligiannidou, Georgia Erini and Havlik, Jaroslav and Janeva, Milkica and Kistanova, Elena and Kontogiorgis, Christos and Krga, Irena and Massaro, Marika and Miler, Marko and Harnafi, Hicham and Milosevic, Verica and Morand, Christine and Scoditti, Egeria and Suárez, Manuel and Vauzour, David and Milenkovic, Dragan",
year = "2022",
abstract = "Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.",
publisher = "Elsevier",
journal = "Ageing Research Reviews, Ageing Research Reviews",
title = "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans – Evidence from untargeted mRNA and miRNA studies",
number = "101649",
volume = "79",
doi = "10.1016/j.arr.2022.101649"
}

Ruskovska, T., Budić-Leto, I., Corral-Jara, K. F., Ajdžanović, V., Arola-Arnal, A., Bravo, F. I., Deligiannidou, G. E., Havlik, J., Janeva, M., Kistanova, E., Kontogiorgis, C., Krga, I., Massaro, M., Miler, M., Harnafi, H., Milosevic, V., Morand, C., Scoditti, E., Suárez, M., Vauzour, D.,& Milenkovic, D.. (2022). Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans – Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews
Elsevier., 79(101649).
https://doi.org/10.1016/j.arr.2022.101649

Ruskovska T, Budić-Leto I, Corral-Jara KF, Ajdžanović V, Arola-Arnal A, Bravo FI, Deligiannidou GE, Havlik J, Janeva M, Kistanova E, Kontogiorgis C, Krga I, Massaro M, Miler M, Harnafi H, Milosevic V, Morand C, Scoditti E, Suárez M, Vauzour D, Milenkovic D. Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans – Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews. 2022;79(101649).
doi:10.1016/j.arr.2022.101649 .

Ruskovska, Tatjana, Budić-Leto, Irena, Corral-Jara, Karla Fabiola, Ajdžanović, Vladimir, Arola-Arnal, Anna, Bravo, Francisca Isabel, Deligiannidou, Georgia Erini, Havlik, Jaroslav, Janeva, Milkica, Kistanova, Elena, Kontogiorgis, Christos, Krga, Irena, Massaro, Marika, Miler, Marko, Harnafi, Hicham, Milosevic, Verica, Morand, Christine, Scoditti, Egeria, Suárez, Manuel, Vauzour, David, Milenkovic, Dragan, "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans – Evidence from untargeted mRNA and miRNA studies" in Ageing Research Reviews, 79, no. 101649 (2022),
https://doi.org/10.1016/j.arr.2022.101649 . .

TY  - CONF
AU  - Krga, Irena
AU  - Zec, Manja
AU  - Takić, Marija
AU  - Debeljak-Martačić, Jasmina
AU  - Korićanac, Goran
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Glibetić, Marija
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1384
PB  - Belgrade : University of Belgrade
C3  - UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts
T1  - Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1384
ER  - 

@conference{
author = "Krga, Irena and Zec, Manja and Takić, Marija and Debeljak-Martačić, Jasmina and Korićanac, Goran and Ranković, Slavica and Popović, Tamara and Glibetić, Marija",
year = "2021",
publisher = "Belgrade : University of Belgrade",
journal = "UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts",
title = "Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1384"
}

Krga, I., Zec, M., Takić, M., Debeljak-Martačić, J., Korićanac, G., Ranković, S., Popović, T.,& Glibetić, M.. (2021). Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats. in UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts
Belgrade : University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimi_1384

Krga I, Zec M, Takić M, Debeljak-Martačić J, Korićanac G, Ranković S, Popović T, Glibetić M. Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats. in UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_rimi_1384 .

Krga, Irena, Zec, Manja, Takić, Marija, Debeljak-Martačić, Jasmina, Korićanac, Goran, Ranković, Slavica, Popović, Tamara, Glibetić, Marija, "Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats" in UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts (2021),
https://hdl.handle.net/21.15107/rcub_rimi_1384 .

TY  - JOUR
AU  - Ramaccini, Daniela
AU  - Montoya-Uribe, Vanessa
AU  - Aan, Femke J.
AU  - Modesti, Lorenzo
AU  - Potes, Yaiza
AU  - Wieckowski, Mariusz R.
AU  - Krga, Irena
AU  - Glibetić, Marija
AU  - Pinton, Paolo
AU  - Giorgi, Carlotta
AU  - Matter, Michelle L.
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1079
AB  - Cardiac tissue requires a persistent production of energy in order to exert its pumping function. Therefore, the maintenance of this function relies on mitochondria that represent the "powerhouse" of all cardiac activities. Mitochondria being one of the key players for the proper functioning of the mammalian heart suggests continual regulation and organization. Mitochondria adapt to cellular energy demands via fusion-fission events and, as a proof-reading ability, undergo mitophagy in cases of abnormalities. Ca2+ fluxes play a pivotal role in regulating all mitochondrial functions, including ATP production, metabolism, oxidative stress balance and apoptosis. Communication between mitochondria and others organelles, especially the sarcoplasmic reticulum is required for optimal function. Consequently, abnormal mitochondrial activity results in decreased energy production leading to pathological conditions. In this review, we will describe how mitochondrial function or dysfunction impacts cardiac activities and the development of dilated cardiomyopathy.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cell and Developmental Biology
T1  - Mitochondrial Function and Dysfunction in Dilated Cardiomyopathy
SP  - 624216
VL  - 8
DO  - 10.3389/fcell.2020.624216
ER  - 

@article{
author = "Ramaccini, Daniela and Montoya-Uribe, Vanessa and Aan, Femke J. and Modesti, Lorenzo and Potes, Yaiza and Wieckowski, Mariusz R. and Krga, Irena and Glibetić, Marija and Pinton, Paolo and Giorgi, Carlotta and Matter, Michelle L.",
year = "2021",
abstract = "Cardiac tissue requires a persistent production of energy in order to exert its pumping function. Therefore, the maintenance of this function relies on mitochondria that represent the "powerhouse" of all cardiac activities. Mitochondria being one of the key players for the proper functioning of the mammalian heart suggests continual regulation and organization. Mitochondria adapt to cellular energy demands via fusion-fission events and, as a proof-reading ability, undergo mitophagy in cases of abnormalities. Ca2+ fluxes play a pivotal role in regulating all mitochondrial functions, including ATP production, metabolism, oxidative stress balance and apoptosis. Communication between mitochondria and others organelles, especially the sarcoplasmic reticulum is required for optimal function. Consequently, abnormal mitochondrial activity results in decreased energy production leading to pathological conditions. In this review, we will describe how mitochondrial function or dysfunction impacts cardiac activities and the development of dilated cardiomyopathy.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cell and Developmental Biology",
title = "Mitochondrial Function and Dysfunction in Dilated Cardiomyopathy",
pages = "624216",
volume = "8",
doi = "10.3389/fcell.2020.624216"
}

Ramaccini, D., Montoya-Uribe, V., Aan, F. J., Modesti, L., Potes, Y., Wieckowski, M. R., Krga, I., Glibetić, M., Pinton, P., Giorgi, C.,& Matter, M. L.. (2021). Mitochondrial Function and Dysfunction in Dilated Cardiomyopathy. in Frontiers in Cell and Developmental Biology
Frontiers Media Sa, Lausanne., 8, 624216.
https://doi.org/10.3389/fcell.2020.624216

Ramaccini D, Montoya-Uribe V, Aan FJ, Modesti L, Potes Y, Wieckowski MR, Krga I, Glibetić M, Pinton P, Giorgi C, Matter ML. Mitochondrial Function and Dysfunction in Dilated Cardiomyopathy. in Frontiers in Cell and Developmental Biology. 2021;8:624216.
doi:10.3389/fcell.2020.624216 .

Ramaccini, Daniela, Montoya-Uribe, Vanessa, Aan, Femke J., Modesti, Lorenzo, Potes, Yaiza, Wieckowski, Mariusz R., Krga, Irena, Glibetić, Marija, Pinton, Paolo, Giorgi, Carlotta, Matter, Michelle L., "Mitochondrial Function and Dysfunction in Dilated Cardiomyopathy" in Frontiers in Cell and Developmental Biology, 8 (2021):624216,
https://doi.org/10.3389/fcell.2020.624216 . .

TY  - JOUR
AU  - Zec, Manja M.
AU  - Krga, Irena
AU  - Stojković, Ljiljana
AU  - Živković, Maja
AU  - Pokimica, Biljana
AU  - Stanković, Aleksandra
AU  - Glibetić, Marija
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1085
AB  - Dietary polyphenols promote cardiometabolic health and are linked with long-chain polyunsaturated fatty acids in plasma phospholipids (LC-PUFA). The FADS2 polymorphisms are associated with LC-PUFA metabolism and overweight/obesity. This 4-week study examined the link between polyphenol intake, FADS2 variants (rs174593, rs174616, rs174576) and obesity in 62 overweight adults (BMI  gt = 25), allocated to consume 100 mL daily of either: Aronia juice, a rich source of polyphenols, with 1177.11 mg polyphenols (expressed as gallic acid equivalents)/100 mL (AJ, n = 22), Aronia juice with 294.28 mg polyphenols/100 mL (MJ, n = 20), or nutritionally matched polyphenol-lacking placebo as a control (PLB, n = 20). We analyzed LC-PUFA (% of total pool) by gas chromatography and FADS2 variants by real-time PCR. Four-week changes in LC-PUFA, BMI, and body weight were included in statistical models, controlling for gender and PUFA intake. Only upon AJ and MJ, the presence of FADS2 variant alleles affected changes in linoleic, arachidonic, and eicosapentaenoic acid (EPA). Upon MJ treatment, changes in EPA were inversely linked with changes in BMI (beta= -0.73, p = 0.029) and weight gain (beta= -2.17, p = 0.024). Only in subjects drinking AJ, the link between changes in EPA and anthropometric indices was modified by the rs174576 variant allele. Our results indicate the interaction between FADS2, fatty acid metabolism, and polyphenol intake in overweight subjects.
PB  - MDPI, Basel
T2  - Nutrients
T1  - Is There a FADS2-Modulated Link between Long-Chain Polyunsaturated Fatty Acids in Plasma Phospholipids and Polyphenol Intake in Adult Subjects Who Are Overweight?
IS  - 2
SP  - 296
VL  - 13
DO  - 10.3390/nu13020296
ER  - 

@article{
author = "Zec, Manja M. and Krga, Irena and Stojković, Ljiljana and Živković, Maja and Pokimica, Biljana and Stanković, Aleksandra and Glibetić, Marija",
year = "2021",
abstract = "Dietary polyphenols promote cardiometabolic health and are linked with long-chain polyunsaturated fatty acids in plasma phospholipids (LC-PUFA). The FADS2 polymorphisms are associated with LC-PUFA metabolism and overweight/obesity. This 4-week study examined the link between polyphenol intake, FADS2 variants (rs174593, rs174616, rs174576) and obesity in 62 overweight adults (BMI  gt = 25), allocated to consume 100 mL daily of either: Aronia juice, a rich source of polyphenols, with 1177.11 mg polyphenols (expressed as gallic acid equivalents)/100 mL (AJ, n = 22), Aronia juice with 294.28 mg polyphenols/100 mL (MJ, n = 20), or nutritionally matched polyphenol-lacking placebo as a control (PLB, n = 20). We analyzed LC-PUFA (% of total pool) by gas chromatography and FADS2 variants by real-time PCR. Four-week changes in LC-PUFA, BMI, and body weight were included in statistical models, controlling for gender and PUFA intake. Only upon AJ and MJ, the presence of FADS2 variant alleles affected changes in linoleic, arachidonic, and eicosapentaenoic acid (EPA). Upon MJ treatment, changes in EPA were inversely linked with changes in BMI (beta= -0.73, p = 0.029) and weight gain (beta= -2.17, p = 0.024). Only in subjects drinking AJ, the link between changes in EPA and anthropometric indices was modified by the rs174576 variant allele. Our results indicate the interaction between FADS2, fatty acid metabolism, and polyphenol intake in overweight subjects.",
publisher = "MDPI, Basel",
journal = "Nutrients",
title = "Is There a FADS2-Modulated Link between Long-Chain Polyunsaturated Fatty Acids in Plasma Phospholipids and Polyphenol Intake in Adult Subjects Who Are Overweight?",
number = "2",
pages = "296",
volume = "13",
doi = "10.3390/nu13020296"
}

Zec, M. M., Krga, I., Stojković, L., Živković, M., Pokimica, B., Stanković, A.,& Glibetić, M.. (2021). Is There a FADS2-Modulated Link between Long-Chain Polyunsaturated Fatty Acids in Plasma Phospholipids and Polyphenol Intake in Adult Subjects Who Are Overweight?. in Nutrients
MDPI, Basel., 13(2), 296.
https://doi.org/10.3390/nu13020296

Zec MM, Krga I, Stojković L, Živković M, Pokimica B, Stanković A, Glibetić M. Is There a FADS2-Modulated Link between Long-Chain Polyunsaturated Fatty Acids in Plasma Phospholipids and Polyphenol Intake in Adult Subjects Who Are Overweight?. in Nutrients. 2021;13(2):296.
doi:10.3390/nu13020296 .

Zec, Manja M., Krga, Irena, Stojković, Ljiljana, Živković, Maja, Pokimica, Biljana, Stanković, Aleksandra, Glibetić, Marija, "Is There a FADS2-Modulated Link between Long-Chain Polyunsaturated Fatty Acids in Plasma Phospholipids and Polyphenol Intake in Adult Subjects Who Are Overweight?" in Nutrients, 13, no. 2 (2021):296,
https://doi.org/10.3390/nu13020296 . .

TY  - JOUR
AU  - Milenković, Dragan
AU  - Krga, Irena
AU  - Dinel, Anne-Laure
AU  - Morand, Christine
AU  - Laye, Sophie
AU  - Castanon, Nathalie
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1146
AB  - Dietary anthocyanins may slow cognitive decline, improve cognitive performance and exert neuroprotective effects against neurodegenerative disorders. However, the underlying mechanisms of their action are not fully understood. This study investigated the effects of 12-week anthocyanin-rich bilberry extract supplementation (0.02%) on global gene expression in the hippocampus of ApoE-/- mice to help the understanding of molecular mechanisms underlying anthocyanin neuroprotective effects. Gene expression analysis identified 1698 differently expressed genes, with 611 downregulated and 1087 upregulated genes. Bioinformatics revealed that these genes regulate different biological processes, including neurogenesis, inflammation, metabolism, cell to cell adhesion, cytoskeleton organization, and Alzheimer's and Parkinson's disease pathology. The bioinformatic analysis also proposed potential miRNAs and transcription factors that could be involved in the mediation of these nutrigenomic effects. Results from molecular docking also suggested that anthocyanins could bind to top transcription factors with, as potential consequence, an impact on their gene expression regulation. Taken together, integrated analysis revealed a multi-target mode of action of anthocyanin-rich bilberry extract in the hippocampus underlying their neuroprotective properties.
PB  - Elsevier
T2  - Journal of Functional Foods
T1  - Nutrigenomic modification induced by anthocyanin-rich bilberry extract in the hippocampus of ApoE-/- mice
SP  - 104609
VL  - 85
DO  - 10.1016/j.jff.2021.104609
ER  - 

@article{
author = "Milenković, Dragan and Krga, Irena and Dinel, Anne-Laure and Morand, Christine and Laye, Sophie and Castanon, Nathalie",
year = "2021",
abstract = "Dietary anthocyanins may slow cognitive decline, improve cognitive performance and exert neuroprotective effects against neurodegenerative disorders. However, the underlying mechanisms of their action are not fully understood. This study investigated the effects of 12-week anthocyanin-rich bilberry extract supplementation (0.02%) on global gene expression in the hippocampus of ApoE-/- mice to help the understanding of molecular mechanisms underlying anthocyanin neuroprotective effects. Gene expression analysis identified 1698 differently expressed genes, with 611 downregulated and 1087 upregulated genes. Bioinformatics revealed that these genes regulate different biological processes, including neurogenesis, inflammation, metabolism, cell to cell adhesion, cytoskeleton organization, and Alzheimer's and Parkinson's disease pathology. The bioinformatic analysis also proposed potential miRNAs and transcription factors that could be involved in the mediation of these nutrigenomic effects. Results from molecular docking also suggested that anthocyanins could bind to top transcription factors with, as potential consequence, an impact on their gene expression regulation. Taken together, integrated analysis revealed a multi-target mode of action of anthocyanin-rich bilberry extract in the hippocampus underlying their neuroprotective properties.",
publisher = "Elsevier",
journal = "Journal of Functional Foods",
title = "Nutrigenomic modification induced by anthocyanin-rich bilberry extract in the hippocampus of ApoE-/- mice",
pages = "104609",
volume = "85",
doi = "10.1016/j.jff.2021.104609"
}

Milenković, D., Krga, I., Dinel, A., Morand, C., Laye, S.,& Castanon, N.. (2021). Nutrigenomic modification induced by anthocyanin-rich bilberry extract in the hippocampus of ApoE-/- mice. in Journal of Functional Foods
Elsevier., 85, 104609.
https://doi.org/10.1016/j.jff.2021.104609

Milenković D, Krga I, Dinel A, Morand C, Laye S, Castanon N. Nutrigenomic modification induced by anthocyanin-rich bilberry extract in the hippocampus of ApoE-/- mice. in Journal of Functional Foods. 2021;85:104609.
doi:10.1016/j.jff.2021.104609 .

Milenković, Dragan, Krga, Irena, Dinel, Anne-Laure, Morand, Christine, Laye, Sophie, Castanon, Nathalie, "Nutrigenomic modification induced by anthocyanin-rich bilberry extract in the hippocampus of ApoE-/- mice" in Journal of Functional Foods, 85 (2021):104609,
https://doi.org/10.1016/j.jff.2021.104609 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Budić-Leto, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Ajdžanović, Vladimir
AU  - Arola-Arnal, Anna
AU  - Bravo, Francisca Isabel
AU  - Deligiannidou, Georgia-Eirini
AU  - Havlik, Jaroslav
AU  - Janeva, Milkica
AU  - Kistanova, Elena
AU  - Kontogiorgis, Christos
AU  - Krga, Irena
AU  - Massaro, Marika
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Morand, Christine
AU  - Scoditti, Egeria
AU  - Suárez, Manuel
AU  - Vauzour, David
AU  - Milenković, Dragan
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1147
AB  - Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.
PB  - MDPI
T2  - Nutrients
T1  - Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies
IS  - 7
SP  - 2326
VL  - 13
DO  - 10.3390/nu13072326
ER  - 

@article{
author = "Ruskovska, Tatjana and Budić-Leto, Irena and Corral-Jara, Karla Fabiola and Ajdžanović, Vladimir and Arola-Arnal, Anna and Bravo, Francisca Isabel and Deligiannidou, Georgia-Eirini and Havlik, Jaroslav and Janeva, Milkica and Kistanova, Elena and Kontogiorgis, Christos and Krga, Irena and Massaro, Marika and Miler, Marko and Milošević, Verica and Morand, Christine and Scoditti, Egeria and Suárez, Manuel and Vauzour, David and Milenković, Dragan",
year = "2021",
abstract = "Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.",
publisher = "MDPI",
journal = "Nutrients",
title = "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies",
number = "7",
pages = "2326",
volume = "13",
doi = "10.3390/nu13072326"
}

Ruskovska, T., Budić-Leto, I., Corral-Jara, K. F., Ajdžanović, V., Arola-Arnal, A., Bravo, F. I., Deligiannidou, G., Havlik, J., Janeva, M., Kistanova, E., Kontogiorgis, C., Krga, I., Massaro, M., Miler, M., Milošević, V., Morand, C., Scoditti, E., Suárez, M., Vauzour, D.,& Milenković, D.. (2021). Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies. in Nutrients
MDPI., 13(7), 2326.
https://doi.org/10.3390/nu13072326

Ruskovska T, Budić-Leto I, Corral-Jara KF, Ajdžanović V, Arola-Arnal A, Bravo FI, Deligiannidou G, Havlik J, Janeva M, Kistanova E, Kontogiorgis C, Krga I, Massaro M, Miler M, Milošević V, Morand C, Scoditti E, Suárez M, Vauzour D, Milenković D. Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies. in Nutrients. 2021;13(7):2326.
doi:10.3390/nu13072326 .

Ruskovska, Tatjana, Budić-Leto, Irena, Corral-Jara, Karla Fabiola, Ajdžanović, Vladimir, Arola-Arnal, Anna, Bravo, Francisca Isabel, Deligiannidou, Georgia-Eirini, Havlik, Jaroslav, Janeva, Milkica, Kistanova, Elena, Kontogiorgis, Christos, Krga, Irena, Massaro, Marika, Miler, Marko, Milošević, Verica, Morand, Christine, Scoditti, Egeria, Suárez, Manuel, Vauzour, David, Milenković, Dragan, "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies" in Nutrients, 13, no. 7 (2021):2326,
https://doi.org/10.3390/nu13072326 . .

TY  - JOUR
AU  - Monfoulet, Laurent-Emmanuel
AU  - Ruskovska, Tatjana
AU  - Ajdžanović, Vladimir
AU  - Havlik, Jaroslav
AU  - Vauzour, David
AU  - Bayram, Banu
AU  - Krga, Irena
AU  - Corral-Jara, Karla-Fabiola
AU  - Kistanova, Elena
AU  - Abadjieva, Desislava
AU  - Massaro, Marika
AU  - Scoditti, Egeria
AU  - Deligiannidou, Eirini
AU  - Kontogiorgis, Christos
AU  - Arola-Arnal, Anna
AU  - van Schothorst, Evert M.
AU  - Morand, Christine
AU  - Milenković, Dragan
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1158
AB  - Scope: Flavanols are important polyphenols of the human diet with extensive demonstrations of their beneficial effects on cardiometabolic health. They contribute to preserve health acting on a large range of cellular processes. The underlying mechanisms of action of flavanols are not fully understood but involve a nutrigenomic regulation. Methods and Results: To further capture how the intake of dietary flavanols results in the modulation of gene expression, nutrigenomics data in response to dietary flavanols obtained from animal models of cardiometabolic diseases have been collected and submitted to a bioinformatics analysis. This systematic analysis shows that dietary flavanols modulate a large range of genes mainly involved in endocrine function, fatty acid metabolism, and inflammation. Several regulators of the gene expression have been predicted and include transcription factors, miRNAs and epigenetic factors. Conclusion: This review highlights the complex and multilevel action of dietary flavanols contributing to their strong potential to preserve cardiometabolic health. The identification of the potential molecular mediators and of the flavanol metabolites driving the nutrigenomic response in the target organs is still a pending question which the answer will contribute to optimize the beneficial health effects of dietary bioactives.
PB  - Wiley-Blackwell
T2  - Molecular Nutrition & Food Research
T1  - Molecular Determinants of the Cardiometabolic Improvements of Dietary Flavanols Identified by an Integrative Analysis of Nutrigenomic Data from a Systematic Review of Animal Studies
IS  - 16
SP  - 2100227
VL  - 65
DO  - 10.1002/mnfr.202100227
ER  - 

@article{
author = "Monfoulet, Laurent-Emmanuel and Ruskovska, Tatjana and Ajdžanović, Vladimir and Havlik, Jaroslav and Vauzour, David and Bayram, Banu and Krga, Irena and Corral-Jara, Karla-Fabiola and Kistanova, Elena and Abadjieva, Desislava and Massaro, Marika and Scoditti, Egeria and Deligiannidou, Eirini and Kontogiorgis, Christos and Arola-Arnal, Anna and van Schothorst, Evert M. and Morand, Christine and Milenković, Dragan",
year = "2021",
abstract = "Scope: Flavanols are important polyphenols of the human diet with extensive demonstrations of their beneficial effects on cardiometabolic health. They contribute to preserve health acting on a large range of cellular processes. The underlying mechanisms of action of flavanols are not fully understood but involve a nutrigenomic regulation. Methods and Results: To further capture how the intake of dietary flavanols results in the modulation of gene expression, nutrigenomics data in response to dietary flavanols obtained from animal models of cardiometabolic diseases have been collected and submitted to a bioinformatics analysis. This systematic analysis shows that dietary flavanols modulate a large range of genes mainly involved in endocrine function, fatty acid metabolism, and inflammation. Several regulators of the gene expression have been predicted and include transcription factors, miRNAs and epigenetic factors. Conclusion: This review highlights the complex and multilevel action of dietary flavanols contributing to their strong potential to preserve cardiometabolic health. The identification of the potential molecular mediators and of the flavanol metabolites driving the nutrigenomic response in the target organs is still a pending question which the answer will contribute to optimize the beneficial health effects of dietary bioactives.",
publisher = "Wiley-Blackwell",
journal = "Molecular Nutrition & Food Research",
title = "Molecular Determinants of the Cardiometabolic Improvements of Dietary Flavanols Identified by an Integrative Analysis of Nutrigenomic Data from a Systematic Review of Animal Studies",
number = "16",
pages = "2100227",
volume = "65",
doi = "10.1002/mnfr.202100227"
}

Monfoulet, L., Ruskovska, T., Ajdžanović, V., Havlik, J., Vauzour, D., Bayram, B., Krga, I., Corral-Jara, K., Kistanova, E., Abadjieva, D., Massaro, M., Scoditti, E., Deligiannidou, E., Kontogiorgis, C., Arola-Arnal, A., van Schothorst, E. M., Morand, C.,& Milenković, D.. (2021). Molecular Determinants of the Cardiometabolic Improvements of Dietary Flavanols Identified by an Integrative Analysis of Nutrigenomic Data from a Systematic Review of Animal Studies. in Molecular Nutrition & Food Research
Wiley-Blackwell., 65(16), 2100227.
https://doi.org/10.1002/mnfr.202100227

Monfoulet L, Ruskovska T, Ajdžanović V, Havlik J, Vauzour D, Bayram B, Krga I, Corral-Jara K, Kistanova E, Abadjieva D, Massaro M, Scoditti E, Deligiannidou E, Kontogiorgis C, Arola-Arnal A, van Schothorst EM, Morand C, Milenković D. Molecular Determinants of the Cardiometabolic Improvements of Dietary Flavanols Identified by an Integrative Analysis of Nutrigenomic Data from a Systematic Review of Animal Studies. in Molecular Nutrition & Food Research. 2021;65(16):2100227.
doi:10.1002/mnfr.202100227 .

Monfoulet, Laurent-Emmanuel, Ruskovska, Tatjana, Ajdžanović, Vladimir, Havlik, Jaroslav, Vauzour, David, Bayram, Banu, Krga, Irena, Corral-Jara, Karla-Fabiola, Kistanova, Elena, Abadjieva, Desislava, Massaro, Marika, Scoditti, Egeria, Deligiannidou, Eirini, Kontogiorgis, Christos, Arola-Arnal, Anna, van Schothorst, Evert M., Morand, Christine, Milenković, Dragan, "Molecular Determinants of the Cardiometabolic Improvements of Dietary Flavanols Identified by an Integrative Analysis of Nutrigenomic Data from a Systematic Review of Animal Studies" in Molecular Nutrition & Food Research, 65, no. 16 (2021):2100227,
https://doi.org/10.1002/mnfr.202100227 . .

TY  - JOUR
AU  - Bošković, Maja
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanišić, Jelena
AU  - Zec, Manja
AU  - Krga, Irena
AU  - Stanković, Aleksandra
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1144
AB  - The benefits of walnut (Juglans regia) consumption for metabolic health are known, but the molecular background underlying their putative antioxidant and anti-inflammatory/immunomodulatory effects is underexplored. We assessed that walnut supplementation (6 weeks) reverted unfavorable changes of the SIRT1/FoxO3a/MnSOD/catalase axis in the heart induced by fructose-rich diet (FRD). Intriguingly, Nox4 was increased by both FRD and walnut supplementation. FRD increased the cytosolic fraction and decreased the nuclear fraction of the uniquely elucidated ChREBP in the heart. The ChREBP nuclear fraction was decreased in control rats subjected to walnuts. In addition, walnut consumption was associated with a reduction in systolic BP in FRD and a decrease in fatty acid AA/EPA and AA/DHA ratios in plasma. In summary, the protective effect of walnut supplementation was detected in male rats following the fructose-induced decrease in antioxidative/anti-inflammatory capacity of cardiac tissue and increase in plasma predictors of low-grade inflammation. The current results provide a novel insight into the relationship between nutrients, cellular energy homeostasis, and the modulators of inflammatory/immune response in metabolic syndrome, emphasizing the heart and highlighting a track for translation into nutrition and dietary therapeutic approaches against metabolic disease.
PB  - Hindawi
T2  - Oxidative Medicine and Cellular Longevity
T1  - Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet
SP  - e5543025
VL  - 2021
DO  - 10.1155/2021/5543025
ER  - 

@article{
author = "Bošković, Maja and Živković, Maja and Korićanac, Goran and Stanišić, Jelena and Zec, Manja and Krga, Irena and Stanković, Aleksandra",
year = "2021",
abstract = "The benefits of walnut (Juglans regia) consumption for metabolic health are known, but the molecular background underlying their putative antioxidant and anti-inflammatory/immunomodulatory effects is underexplored. We assessed that walnut supplementation (6 weeks) reverted unfavorable changes of the SIRT1/FoxO3a/MnSOD/catalase axis in the heart induced by fructose-rich diet (FRD). Intriguingly, Nox4 was increased by both FRD and walnut supplementation. FRD increased the cytosolic fraction and decreased the nuclear fraction of the uniquely elucidated ChREBP in the heart. The ChREBP nuclear fraction was decreased in control rats subjected to walnuts. In addition, walnut consumption was associated with a reduction in systolic BP in FRD and a decrease in fatty acid AA/EPA and AA/DHA ratios in plasma. In summary, the protective effect of walnut supplementation was detected in male rats following the fructose-induced decrease in antioxidative/anti-inflammatory capacity of cardiac tissue and increase in plasma predictors of low-grade inflammation. The current results provide a novel insight into the relationship between nutrients, cellular energy homeostasis, and the modulators of inflammatory/immune response in metabolic syndrome, emphasizing the heart and highlighting a track for translation into nutrition and dietary therapeutic approaches against metabolic disease.",
publisher = "Hindawi",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet",
pages = "e5543025",
volume = "2021",
doi = "10.1155/2021/5543025"
}

Bošković, M., Živković, M., Korićanac, G., Stanišić, J., Zec, M., Krga, I.,& Stanković, A.. (2021). Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet. in Oxidative Medicine and Cellular Longevity
Hindawi., 2021, e5543025.
https://doi.org/10.1155/2021/5543025

Bošković M, Živković M, Korićanac G, Stanišić J, Zec M, Krga I, Stanković A. Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet. in Oxidative Medicine and Cellular Longevity. 2021;2021:e5543025.
doi:10.1155/2021/5543025 .

Bošković, Maja, Živković, Maja, Korićanac, Goran, Stanišić, Jelena, Zec, Manja, Krga, Irena, Stanković, Aleksandra, "Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet" in Oxidative Medicine and Cellular Longevity, 2021 (2021):e5543025,
https://doi.org/10.1155/2021/5543025 . .

TY  - JOUR
AU  - Zec, Manja M.
AU  - Krga, Irena
AU  - Zandberg, Lizelle
AU  - Smuts, Cornelius M.
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1189
PB  - Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Editorial: Micronutrients and Fatty Acids in Precision Nutrition Strategies
SP  - 788215
VL  - 8
DO  - 10.3389/fnut.2021.788215
ER  - 

@article{
author = "Zec, Manja M. and Krga, Irena and Zandberg, Lizelle and Smuts, Cornelius M.",
year = "2021",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Editorial: Micronutrients and Fatty Acids in Precision Nutrition Strategies",
pages = "788215",
volume = "8",
doi = "10.3389/fnut.2021.788215"
}

Zec, M. M., Krga, I., Zandberg, L.,& Smuts, C. M.. (2021). Editorial: Micronutrients and Fatty Acids in Precision Nutrition Strategies. in Frontiers in Nutrition
Frontiers Media S.A.., 8, 788215.
https://doi.org/10.3389/fnut.2021.788215

Zec MM, Krga I, Zandberg L, Smuts CM. Editorial: Micronutrients and Fatty Acids in Precision Nutrition Strategies. in Frontiers in Nutrition. 2021;8:788215.
doi:10.3389/fnut.2021.788215 .

Zec, Manja M., Krga, Irena, Zandberg, Lizelle, Smuts, Cornelius M., "Editorial: Micronutrients and Fatty Acids in Precision Nutrition Strategies" in Frontiers in Nutrition, 8 (2021):788215,
https://doi.org/10.3389/fnut.2021.788215 . .

TY  - JOUR
AU  - Zec, Manja M.
AU  - Krga, Irena
AU  - Takić, Marija
AU  - Debeljak-Martačić, Jasmina
AU  - Korićanac, Goran
AU  - Ranković, Slavica G.
AU  - Popović, Tamara B.
AU  - Pantelić, Marija
AU  - Glibetić, Marija
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/984
AB  - Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats
EP  - 28145
IS  - 43
SP  - 28136
VL  - 5
DO  - 10.1021/acsomega.0c03784
ER  - 

@article{
author = "Zec, Manja M. and Krga, Irena and Takić, Marija and Debeljak-Martačić, Jasmina and Korićanac, Goran and Ranković, Slavica G. and Popović, Tamara B. and Pantelić, Marija and Glibetić, Marija",
year = "2020",
abstract = "Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats",
pages = "28145-28136",
number = "43",
volume = "5",
doi = "10.1021/acsomega.0c03784"
}

Zec, M. M., Krga, I., Takić, M., Debeljak-Martačić, J., Korićanac, G., Ranković, S. G., Popović, T. B., Pantelić, M.,& Glibetić, M.. (2020). Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega
Amer Chemical Soc, Washington., 5(43), 28136-28145.
https://doi.org/10.1021/acsomega.0c03784

Zec MM, Krga I, Takić M, Debeljak-Martačić J, Korićanac G, Ranković SG, Popović TB, Pantelić M, Glibetić M. Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega. 2020;5(43):28136-28145.
doi:10.1021/acsomega.0c03784 .

Zec, Manja M., Krga, Irena, Takić, Marija, Debeljak-Martačić, Jasmina, Korićanac, Goran, Ranković, Slavica G., Popović, Tamara B., Pantelić, Marija, Glibetić, Marija, "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats" in ACS Omega, 5, no. 43 (2020):28136-28145,
https://doi.org/10.1021/acsomega.0c03784 . .

TY  - JOUR
AU  - Stevanović, Vuk
AU  - Pantović, Ana
AU  - Krga, Irena
AU  - Zeković, Milica
AU  - Šarac, Ivana
AU  - Glibetić, Marija
AU  - Vidović, Nevena Đ.
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1021
AB  - Long-distance running, especially in non-professional runners, can increase cardiac arrest risk by enhancing platelet activation and aggregation. Polyphenols can exert cardioprotective effects by positively influencing platelet function. This study aimed to examine the acute effects of polyphenol-rich aronia juice consumption, before simulation of a half-marathon race, on platelet activation and aggregation with leukocytes in recreational runners. In this acute crossover study,10 healthy male runners (age 30.8 +/- 2.3 years) consumed breakfast with 200 mL, of aronia juice or 200 mL of placebo. They warmed-up and ran a simulated half-marathon race (21.1 km). Blood was collected at baseline, and at 15 min, 1 h, and 24 h after the run. All variables were analyzed with 4 (time) x 2 (group)ANOVA with repeated measures on both factors. Results revealed a significant effect of group on platelet activation parameters: P-selectin and CPIIb-IIIa expressions significantly decreased in the aronia group compared with the placebo group (F-[1.(9]) = 10.282, p = 0.011 and F-[1.9] = 7.860, p = 0.021, respectively). The effect of rime was significant on both platelet aggregation markers: platelet-monocyte and platelet-neutrophil aggregates were significantly lower after the race (F-[(3).7] = 4.227, p = 0.014 and F-[3.7] = 70.065, p = 0.000, respectively), with changes more pronounced in the later. All effects remained when platelets were exposed to an agonist. These results suggest that aronia consumption could counteract the half-marathon race-induced changes in platelet function. Novelty Aronia juice consumption significantly decreased the expression of platelet activation markers but did not affect platelet aggregation. The race itself did significantly reduce platelet-neutrophil aggregation. Aronia juice may serve as a supplement beverage for recreational runners to alleviate enhanced platelet reactivity caused by prolonged running.
PB  - Canadian Science Publishing, Ottawa
T2  - Applied Physiology Nutrition & Metabolism
T1  - Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners
EP  - 400
IS  - 4
SP  - 393
VL  - 45
DO  - 10.1139/apnm-2019-0267
ER  - 

@article{
author = "Stevanović, Vuk and Pantović, Ana and Krga, Irena and Zeković, Milica and Šarac, Ivana and Glibetić, Marija and Vidović, Nevena Đ.",
year = "2020",
abstract = "Long-distance running, especially in non-professional runners, can increase cardiac arrest risk by enhancing platelet activation and aggregation. Polyphenols can exert cardioprotective effects by positively influencing platelet function. This study aimed to examine the acute effects of polyphenol-rich aronia juice consumption, before simulation of a half-marathon race, on platelet activation and aggregation with leukocytes in recreational runners. In this acute crossover study,10 healthy male runners (age 30.8 +/- 2.3 years) consumed breakfast with 200 mL, of aronia juice or 200 mL of placebo. They warmed-up and ran a simulated half-marathon race (21.1 km). Blood was collected at baseline, and at 15 min, 1 h, and 24 h after the run. All variables were analyzed with 4 (time) x 2 (group)ANOVA with repeated measures on both factors. Results revealed a significant effect of group on platelet activation parameters: P-selectin and CPIIb-IIIa expressions significantly decreased in the aronia group compared with the placebo group (F-[1.(9]) = 10.282, p = 0.011 and F-[1.9] = 7.860, p = 0.021, respectively). The effect of rime was significant on both platelet aggregation markers: platelet-monocyte and platelet-neutrophil aggregates were significantly lower after the race (F-[(3).7] = 4.227, p = 0.014 and F-[3.7] = 70.065, p = 0.000, respectively), with changes more pronounced in the later. All effects remained when platelets were exposed to an agonist. These results suggest that aronia consumption could counteract the half-marathon race-induced changes in platelet function. Novelty Aronia juice consumption significantly decreased the expression of platelet activation markers but did not affect platelet aggregation. The race itself did significantly reduce platelet-neutrophil aggregation. Aronia juice may serve as a supplement beverage for recreational runners to alleviate enhanced platelet reactivity caused by prolonged running.",
publisher = "Canadian Science Publishing, Ottawa",
journal = "Applied Physiology Nutrition & Metabolism",
title = "Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners",
pages = "400-393",
number = "4",
volume = "45",
doi = "10.1139/apnm-2019-0267"
}

Stevanović, V., Pantović, A., Krga, I., Zeković, M., Šarac, I., Glibetić, M.,& Vidović, N. Đ.. (2020). Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners. in Applied Physiology Nutrition & Metabolism
Canadian Science Publishing, Ottawa., 45(4), 393-400.
https://doi.org/10.1139/apnm-2019-0267

Stevanović V, Pantović A, Krga I, Zeković M, Šarac I, Glibetić M, Vidović NĐ. Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners. in Applied Physiology Nutrition & Metabolism. 2020;45(4):393-400.
doi:10.1139/apnm-2019-0267 .

Stevanović, Vuk, Pantović, Ana, Krga, Irena, Zeković, Milica, Šarac, Ivana, Glibetić, Marija, Vidović, Nevena Đ., "Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners" in Applied Physiology Nutrition & Metabolism, 45, no. 4 (2020):393-400,
https://doi.org/10.1139/apnm-2019-0267 . .

TY  - JOUR
AU  - Pantović, Ana
AU  - Krga, Irena
AU  - Zeković, Milica
AU  - Šarac, Ivana
AU  - Glibetić, Marija
AU  - Vidović, Nevena Đ.
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1127
AB  - Long-distance running, especially in non-professional runners, can increase cardiac arrest risk by enhancing platelet activation and aggregation. Polyphenols can exert cardioprotective effects by positively influencing platelet function. This study aimed to examine the acute effects of polyphenol-rich aronia juice consumption, before simulation of a half-marathon race, on platelet activation and aggregation with leukocytes in recreational runners. In this acute crossover study,10 healthy male runners (age 30.8 +/- 2.3 years) consumed breakfast with 200 mL, of aronia juice or 200 mL of placebo. They warmed-up and ran a simulated half-marathon race (21.1 km). Blood was collected at baseline, and at 15 min, 1 h, and 24 h after the run. All variables were analyzed with 4 (time) x 2 (group)ANOVA with repeated measures on both factors. Results revealed a significant effect of group on platelet activation parameters: P-selectin and CPIIb-IIIa expressions significantly decreased in the aronia group compared with the placebo group (F-[1.(9]) = 10.282, p = 0.011 and F-[1.9] = 7.860, p = 0.021, respectively). The effect of rime was significant on both platelet aggregation markers: platelet-monocyte and platelet-neutrophil aggregates were significantly lower after the race (F-[(3).7] = 4.227, p = 0.014 and F-[3.7] = 70.065, p = 0.000, respectively), with changes more pronounced in the later. All effects remained when platelets were exposed to an agonist. These results suggest that aronia consumption could counteract the half-marathon race-induced changes in platelet function. Novelty Aronia juice consumption significantly decreased the expression of platelet activation markers but did not affect platelet aggregation. The race itself did significantly reduce platelet-neutrophil aggregation. Aronia juice may serve as a supplement beverage for recreational runners to alleviate enhanced platelet reactivity caused by prolonged running.
PB  - Canadian Science Publishing, Ottawa
T2  - Applied Physiology Nutrition & Metabolism
T1  - Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners
EP  - 400
IS  - 4
SP  - 393
VL  - 45
DO  - 10.1139/apnm-2019-0267
ER  - 

@article{
author = "Pantović, Ana and Krga, Irena and Zeković, Milica and Šarac, Ivana and Glibetić, Marija and Vidović, Nevena Đ.",
year = "2020",
abstract = "Long-distance running, especially in non-professional runners, can increase cardiac arrest risk by enhancing platelet activation and aggregation. Polyphenols can exert cardioprotective effects by positively influencing platelet function. This study aimed to examine the acute effects of polyphenol-rich aronia juice consumption, before simulation of a half-marathon race, on platelet activation and aggregation with leukocytes in recreational runners. In this acute crossover study,10 healthy male runners (age 30.8 +/- 2.3 years) consumed breakfast with 200 mL, of aronia juice or 200 mL of placebo. They warmed-up and ran a simulated half-marathon race (21.1 km). Blood was collected at baseline, and at 15 min, 1 h, and 24 h after the run. All variables were analyzed with 4 (time) x 2 (group)ANOVA with repeated measures on both factors. Results revealed a significant effect of group on platelet activation parameters: P-selectin and CPIIb-IIIa expressions significantly decreased in the aronia group compared with the placebo group (F-[1.(9]) = 10.282, p = 0.011 and F-[1.9] = 7.860, p = 0.021, respectively). The effect of rime was significant on both platelet aggregation markers: platelet-monocyte and platelet-neutrophil aggregates were significantly lower after the race (F-[(3).7] = 4.227, p = 0.014 and F-[3.7] = 70.065, p = 0.000, respectively), with changes more pronounced in the later. All effects remained when platelets were exposed to an agonist. These results suggest that aronia consumption could counteract the half-marathon race-induced changes in platelet function. Novelty Aronia juice consumption significantly decreased the expression of platelet activation markers but did not affect platelet aggregation. The race itself did significantly reduce platelet-neutrophil aggregation. Aronia juice may serve as a supplement beverage for recreational runners to alleviate enhanced platelet reactivity caused by prolonged running.",
publisher = "Canadian Science Publishing, Ottawa",
journal = "Applied Physiology Nutrition & Metabolism",
title = "Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners",
pages = "400-393",
number = "4",
volume = "45",
doi = "10.1139/apnm-2019-0267"
}

Pantović, A., Krga, I., Zeković, M., Šarac, I., Glibetić, M.,& Vidović, N. Đ.. (2020). Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners. in Applied Physiology Nutrition & Metabolism
Canadian Science Publishing, Ottawa., 45(4), 393-400.
https://doi.org/10.1139/apnm-2019-0267

Pantović A, Krga I, Zeković M, Šarac I, Glibetić M, Vidović NĐ. Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners. in Applied Physiology Nutrition & Metabolism. 2020;45(4):393-400.
doi:10.1139/apnm-2019-0267 .

Pantović, Ana, Krga, Irena, Zeković, Milica, Šarac, Ivana, Glibetić, Marija, Vidović, Nevena Đ., "Aronia juice consumption prior to half-marathon race can acutely affect platelet activation in recreational runners" in Applied Physiology Nutrition & Metabolism, 45, no. 4 (2020):393-400,
https://doi.org/10.1139/apnm-2019-0267 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Massaro, Marika
AU  - Carluccio, Maria Annunziata
AU  - Arola-Arnal, Anna
AU  - Muguerza, Begona
AU  - Vanden Berghe, Wim
AU  - Declerk, Ken
AU  - Isabel Bravo, Francisca
AU  - Calabriso, Nadia
AU  - Combet, Emilie
AU  - Gibney, Eileen R.
AU  - Gomes, Andreia
AU  - Gonthier, Marie-Paule
AU  - Kistanova, Elena
AU  - Krga, Irena
AU  - Mena, Pedro
AU  - Morand, Christine
AU  - dos Santos, Claudia Nunes
AU  - De Pascual-Teresa, Sonia
AU  - Rodriguez-Mateos, Ana
AU  - Scoditti, Egeria
AU  - Suarez, Manuel
AU  - Milenković, Dragan
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1058
AB  - Flavanol intake positively influences several cardiometabolic risk factors in humans. However, the specific molecular mechanisms of action of flavanols, in terms of gene regulation, in the cell types relevant to cardiometabolic disease have never been systematically addressed. On this basis, we conducted a systematic literature review and a comprehensive bioinformatic analysis of genes whose expression is affected by flavanols in cells defining cardiometabolic health: hepatocytes, adipocytes, endothelial cells, smooth muscle cells and immune cells. A systematic literature search was performed using the following pre-defined criteria: treatment with pure compounds and metabolites (no extracts) at low concentrations that are close to their plasma concentrations. Differentially expressed genes were analyzed using bioinformatics tools to identify gene ontologies, networks, cellular pathways and interactions, as well as transcriptional and post-transcriptional regulators. The systematic literature search identified 54 differentially expressed genes at the mRNA level inin vitromodels of cardiometabolic disease exposed to flavanols and their metabolites. Global bioinformatic analysis revealed that these genes are predominantly involved in inflammation, leukocyte adhesion and transendothelial migration, and lipid metabolism. We observed that, although the investigated cells responded differentially to flavanol exposure, the involvement of anti-inflammatory responses is a common mechanism of flavanol action. We also identified potential transcriptional regulators of gene expression: transcriptional factors, such as GATA2, NFKB1, FOXC1 or PPARG, and post-transcriptional regulators: miRNAs, such as mir-335-5p, let-7b-5p, mir-26b-5p or mir-16-5p. In parallel, we analyzed the nutrigenomic effects of flavanols in intestinal cells and demonstrated their predominant involvement in the metabolism of circulating lipoproteins. In conclusion, the results of this systematic analysis of the nutrigenomic effects of flavanols provide a more comprehensive picture of their molecular mechanisms of action and will support the future setup of genetic studies to pave the way for individualized dietary recommendations.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease
EP  - 5064
IS  - 6
SP  - 5040
VL  - 11
DO  - 10.1039/d0fo00701c
ER  - 

@article{
author = "Ruskovska, Tatjana and Massaro, Marika and Carluccio, Maria Annunziata and Arola-Arnal, Anna and Muguerza, Begona and Vanden Berghe, Wim and Declerk, Ken and Isabel Bravo, Francisca and Calabriso, Nadia and Combet, Emilie and Gibney, Eileen R. and Gomes, Andreia and Gonthier, Marie-Paule and Kistanova, Elena and Krga, Irena and Mena, Pedro and Morand, Christine and dos Santos, Claudia Nunes and De Pascual-Teresa, Sonia and Rodriguez-Mateos, Ana and Scoditti, Egeria and Suarez, Manuel and Milenković, Dragan",
year = "2020",
abstract = "Flavanol intake positively influences several cardiometabolic risk factors in humans. However, the specific molecular mechanisms of action of flavanols, in terms of gene regulation, in the cell types relevant to cardiometabolic disease have never been systematically addressed. On this basis, we conducted a systematic literature review and a comprehensive bioinformatic analysis of genes whose expression is affected by flavanols in cells defining cardiometabolic health: hepatocytes, adipocytes, endothelial cells, smooth muscle cells and immune cells. A systematic literature search was performed using the following pre-defined criteria: treatment with pure compounds and metabolites (no extracts) at low concentrations that are close to their plasma concentrations. Differentially expressed genes were analyzed using bioinformatics tools to identify gene ontologies, networks, cellular pathways and interactions, as well as transcriptional and post-transcriptional regulators. The systematic literature search identified 54 differentially expressed genes at the mRNA level inin vitromodels of cardiometabolic disease exposed to flavanols and their metabolites. Global bioinformatic analysis revealed that these genes are predominantly involved in inflammation, leukocyte adhesion and transendothelial migration, and lipid metabolism. We observed that, although the investigated cells responded differentially to flavanol exposure, the involvement of anti-inflammatory responses is a common mechanism of flavanol action. We also identified potential transcriptional regulators of gene expression: transcriptional factors, such as GATA2, NFKB1, FOXC1 or PPARG, and post-transcriptional regulators: miRNAs, such as mir-335-5p, let-7b-5p, mir-26b-5p or mir-16-5p. In parallel, we analyzed the nutrigenomic effects of flavanols in intestinal cells and demonstrated their predominant involvement in the metabolism of circulating lipoproteins. In conclusion, the results of this systematic analysis of the nutrigenomic effects of flavanols provide a more comprehensive picture of their molecular mechanisms of action and will support the future setup of genetic studies to pave the way for individualized dietary recommendations.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease",
pages = "5064-5040",
number = "6",
volume = "11",
doi = "10.1039/d0fo00701c"
}

Ruskovska, T., Massaro, M., Carluccio, M. A., Arola-Arnal, A., Muguerza, B., Vanden Berghe, W., Declerk, K., Isabel Bravo, F., Calabriso, N., Combet, E., Gibney, E. R., Gomes, A., Gonthier, M., Kistanova, E., Krga, I., Mena, P., Morand, C., dos Santos, C. N., De Pascual-Teresa, S., Rodriguez-Mateos, A., Scoditti, E., Suarez, M.,& Milenković, D.. (2020). Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease. in Food & Function
Royal Soc Chemistry, Cambridge., 11(6), 5040-5064.
https://doi.org/10.1039/d0fo00701c

Ruskovska T, Massaro M, Carluccio MA, Arola-Arnal A, Muguerza B, Vanden Berghe W, Declerk K, Isabel Bravo F, Calabriso N, Combet E, Gibney ER, Gomes A, Gonthier M, Kistanova E, Krga I, Mena P, Morand C, dos Santos CN, De Pascual-Teresa S, Rodriguez-Mateos A, Scoditti E, Suarez M, Milenković D. Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease. in Food & Function. 2020;11(6):5040-5064.
doi:10.1039/d0fo00701c .

Ruskovska, Tatjana, Massaro, Marika, Carluccio, Maria Annunziata, Arola-Arnal, Anna, Muguerza, Begona, Vanden Berghe, Wim, Declerk, Ken, Isabel Bravo, Francisca, Calabriso, Nadia, Combet, Emilie, Gibney, Eileen R., Gomes, Andreia, Gonthier, Marie-Paule, Kistanova, Elena, Krga, Irena, Mena, Pedro, Morand, Christine, dos Santos, Claudia Nunes, De Pascual-Teresa, Sonia, Rodriguez-Mateos, Ana, Scoditti, Egeria, Suarez, Manuel, Milenković, Dragan, "Systematic bioinformatic analysis of nutrigenomic data of flavanols in cell models of cardiometabolic disease" in Food & Function, 11, no. 6 (2020):5040-5064,
https://doi.org/10.1039/d0fo00701c . .

TY  - JOUR
AU  - Krga, Irena
AU  - Milenković, Dragan
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/958
AB  - Anthocyanins are phytochemicals widely found in plant foods, with berries and fruit-derived beverages as the main dietary sources. Accumulating evidence suggests the positive role of anthocyanins in preserving cardiovascular health. Epidemiological data show an association between anthocyanin intake and lower risk of myocardial infarction and cardiovascular-disease-related mortality. Clinical studies report the beneficial effects of the consumption of different anthocyanin-rich sources on surrogate markers of cardiovascular risk. Animal and in vitro evidence suggest the protective role of anthocyanins in dysfunctions related to the development of cardiovascular diseases. Still, the underlying molecular mechanisms of anthocyanin action seem complex and are not entirely clear. This review aims to give a comprehensive update on anthocyanins and their cardioprotective properties. It provides information on their sources; quantities consumed through diet; absorption; bioavailability; cardiovascular properties; and underlying mechanisms of action, including their effects on gene and protein expression and their interactions with cell-signaling pathways and miRNAs.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural & Food Chemistry
T1  - Anthocyanins: From Sources and Bioavailability to Cardiovascular-Health Benefits and Molecular Mechanisms of Action
EP  - 1783
IS  - 7
SP  - 1771
VL  - 67
DO  - 10.1021/acs.jafc.8b06737
ER  - 

@article{
author = "Krga, Irena and Milenković, Dragan",
year = "2019",
abstract = "Anthocyanins are phytochemicals widely found in plant foods, with berries and fruit-derived beverages as the main dietary sources. Accumulating evidence suggests the positive role of anthocyanins in preserving cardiovascular health. Epidemiological data show an association between anthocyanin intake and lower risk of myocardial infarction and cardiovascular-disease-related mortality. Clinical studies report the beneficial effects of the consumption of different anthocyanin-rich sources on surrogate markers of cardiovascular risk. Animal and in vitro evidence suggest the protective role of anthocyanins in dysfunctions related to the development of cardiovascular diseases. Still, the underlying molecular mechanisms of anthocyanin action seem complex and are not entirely clear. This review aims to give a comprehensive update on anthocyanins and their cardioprotective properties. It provides information on their sources; quantities consumed through diet; absorption; bioavailability; cardiovascular properties; and underlying mechanisms of action, including their effects on gene and protein expression and their interactions with cell-signaling pathways and miRNAs.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural & Food Chemistry",
title = "Anthocyanins: From Sources and Bioavailability to Cardiovascular-Health Benefits and Molecular Mechanisms of Action",
pages = "1783-1771",
number = "7",
volume = "67",
doi = "10.1021/acs.jafc.8b06737"
}

Krga, I.,& Milenković, D.. (2019). Anthocyanins: From Sources and Bioavailability to Cardiovascular-Health Benefits and Molecular Mechanisms of Action. in Journal of Agricultural & Food Chemistry
Amer Chemical Soc, Washington., 67(7), 1771-1783.
https://doi.org/10.1021/acs.jafc.8b06737

Krga I, Milenković D. Anthocyanins: From Sources and Bioavailability to Cardiovascular-Health Benefits and Molecular Mechanisms of Action. in Journal of Agricultural & Food Chemistry. 2019;67(7):1771-1783.
doi:10.1021/acs.jafc.8b06737 .

Krga, Irena, Milenković, Dragan, "Anthocyanins: From Sources and Bioavailability to Cardiovascular-Health Benefits and Molecular Mechanisms of Action" in Journal of Agricultural & Food Chemistry, 67, no. 7 (2019):1771-1783,
https://doi.org/10.1021/acs.jafc.8b06737 . .

TY  - JOUR
AU  - Nikolić, Marina
AU  - Konić-Ristić, Aleksandra
AU  - Gonzalez-Sarrias, Antonio
AU  - Istas, Geoffrey
AU  - Urpi-Sarda, Mireia
AU  - Dall'Asta, Margherita
AU  - Monfoulet, Laurent-Emanuel
AU  - Cloetens, Lieselotte
AU  - Bayram, Banu
AU  - Tumolo, Maria Rosaria
AU  - Chervenkov, Mihail
AU  - Scoditti, Egeria
AU  - Massaro, Marika
AU  - Tejera, Noemi
AU  - Abadjieva, Desislava
AU  - Chambers, Karen
AU  - Krga, Irena
AU  - Tomas-Barberan, Francisco A.
AU  - Morand, Christine
AU  - Feliciano, Rodrigo
AU  - Garcia-Villalba, Rocio
AU  - Garcia-Aloy, Mar
AU  - Mena, Pedro
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/933
AB  - Key messages The reporting quality of human studies on inter-individual variation in response to plant bioactives is generally low and should be significantly improved. There is no specific guidance for reporting studies on inter-individual variation in response to plant bioactives. The assessment of reporting quality using a scale approach is considered a valuable tool in assessing compliance with the recommendations in the submission phase or during the reviewing process. It also provides a quantitative measure of the quality of studies to be used in meta-analysis. Eleven reporting criteria were developed and supported by detailed definitions and guidance for their scoring. The POSITIVe quality index was tested and demonstrated to be valid, reliable, and responsive. The use of the quality index and its supporting explanatory material (dictionary) as a guide for researchers, peer-reviewers, and journal editors will foster further complete and transparent reporting of data on inter-individual variability. The criteria used in the quality index can serve as additional guidance to inform the design and conduction of further studies on inter-individual variations in response to plant bioactives. Better reporting is expected to lead to a better understanding of the mechanisms and factors involved and thus better study designs with greater impact on policies and practice. Purpose The quality of the study design and data reporting in human trials dealing with the inter-individual variability in response to the consumption of plant bioactives is, in general, low. There is a lack of recommendations supporting the scientific community on this topic. This study aimed at developing a quality index to assist the assessment of the reporting quality of intervention trials addressing the inter-individual variability in response to plant bioactive consumption. Recommendations for better designing and reporting studies were discussed. Methods The selection of the parameters used for the development of the quality index was carried out in agreement with the scientific community through a survey. Parameters were defined, grouped into categories, and scored for different quality levels. The applicability of the scoring system was tested in terms of consistency and effort, and its validity was assessed by comparison with a simultaneous evaluation by experts' criteria. Results The "POSITIVe quality index" included 11 reporting criteria grouped into four categories (Statistics, Reporting, Data presentation, and Individual data availability). It was supported by detailed definitions and guidance for their scoring. The quality index score was tested, and the index demonstrated to be valid, reliable, and responsive. Conclusions The evaluation of the reporting quality of studies addressing inter-individual variability in response to plant bioactives highlighted the aspects requiring major improvements. Specific tools and recommendations favoring a complete and transparent reporting on inter-individual variability have been provided to support the scientific community on this field.
PB  - Springer Heidelberg, Heidelberg
T2  - European Journal of Nutrition
T1  - Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations
EP  - S64
SP  - S49
VL  - 58
DO  - 10.1007/s00394-019-02069-3
ER  - 

@article{
author = "Nikolić, Marina and Konić-Ristić, Aleksandra and Gonzalez-Sarrias, Antonio and Istas, Geoffrey and Urpi-Sarda, Mireia and Dall'Asta, Margherita and Monfoulet, Laurent-Emanuel and Cloetens, Lieselotte and Bayram, Banu and Tumolo, Maria Rosaria and Chervenkov, Mihail and Scoditti, Egeria and Massaro, Marika and Tejera, Noemi and Abadjieva, Desislava and Chambers, Karen and Krga, Irena and Tomas-Barberan, Francisco A. and Morand, Christine and Feliciano, Rodrigo and Garcia-Villalba, Rocio and Garcia-Aloy, Mar and Mena, Pedro",
year = "2019",
abstract = "Key messages The reporting quality of human studies on inter-individual variation in response to plant bioactives is generally low and should be significantly improved. There is no specific guidance for reporting studies on inter-individual variation in response to plant bioactives. The assessment of reporting quality using a scale approach is considered a valuable tool in assessing compliance with the recommendations in the submission phase or during the reviewing process. It also provides a quantitative measure of the quality of studies to be used in meta-analysis. Eleven reporting criteria were developed and supported by detailed definitions and guidance for their scoring. The POSITIVe quality index was tested and demonstrated to be valid, reliable, and responsive. The use of the quality index and its supporting explanatory material (dictionary) as a guide for researchers, peer-reviewers, and journal editors will foster further complete and transparent reporting of data on inter-individual variability. The criteria used in the quality index can serve as additional guidance to inform the design and conduction of further studies on inter-individual variations in response to plant bioactives. Better reporting is expected to lead to a better understanding of the mechanisms and factors involved and thus better study designs with greater impact on policies and practice. Purpose The quality of the study design and data reporting in human trials dealing with the inter-individual variability in response to the consumption of plant bioactives is, in general, low. There is a lack of recommendations supporting the scientific community on this topic. This study aimed at developing a quality index to assist the assessment of the reporting quality of intervention trials addressing the inter-individual variability in response to plant bioactive consumption. Recommendations for better designing and reporting studies were discussed. Methods The selection of the parameters used for the development of the quality index was carried out in agreement with the scientific community through a survey. Parameters were defined, grouped into categories, and scored for different quality levels. The applicability of the scoring system was tested in terms of consistency and effort, and its validity was assessed by comparison with a simultaneous evaluation by experts' criteria. Results The "POSITIVe quality index" included 11 reporting criteria grouped into four categories (Statistics, Reporting, Data presentation, and Individual data availability). It was supported by detailed definitions and guidance for their scoring. The quality index score was tested, and the index demonstrated to be valid, reliable, and responsive. Conclusions The evaluation of the reporting quality of studies addressing inter-individual variability in response to plant bioactives highlighted the aspects requiring major improvements. Specific tools and recommendations favoring a complete and transparent reporting on inter-individual variability have been provided to support the scientific community on this field.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "European Journal of Nutrition",
title = "Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations",
pages = "S64-S49",
volume = "58",
doi = "10.1007/s00394-019-02069-3"
}

Nikolić, M., Konić-Ristić, A., Gonzalez-Sarrias, A., Istas, G., Urpi-Sarda, M., Dall'Asta, M., Monfoulet, L., Cloetens, L., Bayram, B., Tumolo, M. R., Chervenkov, M., Scoditti, E., Massaro, M., Tejera, N., Abadjieva, D., Chambers, K., Krga, I., Tomas-Barberan, F. A., Morand, C., Feliciano, R., Garcia-Villalba, R., Garcia-Aloy, M.,& Mena, P.. (2019). Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations. in European Journal of Nutrition
Springer Heidelberg, Heidelberg., 58, S49-S64.
https://doi.org/10.1007/s00394-019-02069-3

Nikolić M, Konić-Ristić A, Gonzalez-Sarrias A, Istas G, Urpi-Sarda M, Dall'Asta M, Monfoulet L, Cloetens L, Bayram B, Tumolo MR, Chervenkov M, Scoditti E, Massaro M, Tejera N, Abadjieva D, Chambers K, Krga I, Tomas-Barberan FA, Morand C, Feliciano R, Garcia-Villalba R, Garcia-Aloy M, Mena P. Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations. in European Journal of Nutrition. 2019;58:S49-S64.
doi:10.1007/s00394-019-02069-3 .

Nikolić, Marina, Konić-Ristić, Aleksandra, Gonzalez-Sarrias, Antonio, Istas, Geoffrey, Urpi-Sarda, Mireia, Dall'Asta, Margherita, Monfoulet, Laurent-Emanuel, Cloetens, Lieselotte, Bayram, Banu, Tumolo, Maria Rosaria, Chervenkov, Mihail, Scoditti, Egeria, Massaro, Marika, Tejera, Noemi, Abadjieva, Desislava, Chambers, Karen, Krga, Irena, Tomas-Barberan, Francisco A., Morand, Christine, Feliciano, Rodrigo, Garcia-Villalba, Rocio, Garcia-Aloy, Mar, Mena, Pedro, "Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations" in European Journal of Nutrition, 58 (2019):S49-S64,
https://doi.org/10.1007/s00394-019-02069-3 . .

TY  - JOUR
AU  - Krga, Irena
AU  - Vidović, Nevena Đ.
AU  - Milenković, Dragan
AU  - Konić-Ristić, Aleksandra
AU  - Stojanović, Filip
AU  - Morand, Christine
AU  - Glibetić, Marija
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/841
AB  - Accumulating evidence suggests that anthocyanins play an important role in the cardioprotective effects associated with consumption of anthocyanin-rich foods. These benefits may partly be attributed to their effects on platelets, significant contributors to cardiovascular disease development. This study aimed to investigate the impact of physiologically relevant concentrations of anthocyanins and their metabolites on platelet activation and platelet-leukocyte aggregation. Whole blood from seven healthy volunteers was treated with anthocyanins: cyanidin-3-arabinoside, cyanidin-3-glucoside, cyanidin-3-galactoside, delphinidin-3-glucoside and peonidin-3-glucoside at 0.1 mu M concentration or gut metabolites: 4-hydroxybenzaldehyde, protocatechuic, vanillic, ferulic and hippuric acids at 0.5 mu M, 0.2 mu M, 2 mu M, 1 mu M, 2 mu M concentration, respectively. Markers of adenosine diphosphate-induced platelet activation (P-selectin and GPIIb-IIIa expression) and platelet-monocyte and platelet-neutrophil aggregation were analyzed using flow cytometry. Cyanidin-3-arabinoside, delphinidin-3-glucoside, and peonidin-3-glucoside decreased agonist-induced P-selectin expression, while cyanidin-3-galactoside and cyanidin-3-arabinoside reduced platelet-neutrophil aggregation. Hippuric and protocatechuic acids inhibited P-selectin expression, ferulic acid reduced platelet-monocyte aggregation, while 4-hydroxybenzaldehyde affected P-selectin expression, platelet-neutrophil and monocyte aggregation. Only cyanidin-3-glucoside and protocatechuic acid decreased GPIIb-IIIa expression. These results demonstrate the bioactivity of anthocyanins and their gut metabolites at physiologically relevant concentrations on platelet function and interaction with leukocytes, presenting mechanisms by which they contribute to the beneficial effects of habitual consumption of anthocyanin-rich foods on cardiovascular health.
PB  - Elsevier Science Inc, New York
T2  - Archives of Biochemistry & Biophysics
T1  - Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils
EP  - 41
SP  - 34
VL  - 645
DO  - 10.1016/j.abb.2018.03.016
ER  - 

@article{
author = "Krga, Irena and Vidović, Nevena Đ. and Milenković, Dragan and Konić-Ristić, Aleksandra and Stojanović, Filip and Morand, Christine and Glibetić, Marija",
year = "2018",
abstract = "Accumulating evidence suggests that anthocyanins play an important role in the cardioprotective effects associated with consumption of anthocyanin-rich foods. These benefits may partly be attributed to their effects on platelets, significant contributors to cardiovascular disease development. This study aimed to investigate the impact of physiologically relevant concentrations of anthocyanins and their metabolites on platelet activation and platelet-leukocyte aggregation. Whole blood from seven healthy volunteers was treated with anthocyanins: cyanidin-3-arabinoside, cyanidin-3-glucoside, cyanidin-3-galactoside, delphinidin-3-glucoside and peonidin-3-glucoside at 0.1 mu M concentration or gut metabolites: 4-hydroxybenzaldehyde, protocatechuic, vanillic, ferulic and hippuric acids at 0.5 mu M, 0.2 mu M, 2 mu M, 1 mu M, 2 mu M concentration, respectively. Markers of adenosine diphosphate-induced platelet activation (P-selectin and GPIIb-IIIa expression) and platelet-monocyte and platelet-neutrophil aggregation were analyzed using flow cytometry. Cyanidin-3-arabinoside, delphinidin-3-glucoside, and peonidin-3-glucoside decreased agonist-induced P-selectin expression, while cyanidin-3-galactoside and cyanidin-3-arabinoside reduced platelet-neutrophil aggregation. Hippuric and protocatechuic acids inhibited P-selectin expression, ferulic acid reduced platelet-monocyte aggregation, while 4-hydroxybenzaldehyde affected P-selectin expression, platelet-neutrophil and monocyte aggregation. Only cyanidin-3-glucoside and protocatechuic acid decreased GPIIb-IIIa expression. These results demonstrate the bioactivity of anthocyanins and their gut metabolites at physiologically relevant concentrations on platelet function and interaction with leukocytes, presenting mechanisms by which they contribute to the beneficial effects of habitual consumption of anthocyanin-rich foods on cardiovascular health.",
publisher = "Elsevier Science Inc, New York",
journal = "Archives of Biochemistry & Biophysics",
title = "Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils",
pages = "41-34",
volume = "645",
doi = "10.1016/j.abb.2018.03.016"
}

Krga, I., Vidović, N. Đ., Milenković, D., Konić-Ristić, A., Stojanović, F., Morand, C.,& Glibetić, M.. (2018). Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils. in Archives of Biochemistry & Biophysics
Elsevier Science Inc, New York., 645, 34-41.
https://doi.org/10.1016/j.abb.2018.03.016

Krga I, Vidović NĐ, Milenković D, Konić-Ristić A, Stojanović F, Morand C, Glibetić M. Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils. in Archives of Biochemistry & Biophysics. 2018;645:34-41.
doi:10.1016/j.abb.2018.03.016 .

Krga, Irena, Vidović, Nevena Đ., Milenković, Dragan, Konić-Ristić, Aleksandra, Stojanović, Filip, Morand, Christine, Glibetić, Marija, "Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils" in Archives of Biochemistry & Biophysics, 645 (2018):34-41,
https://doi.org/10.1016/j.abb.2018.03.016 . .

TY  - JOUR
AU  - Krga, Irena
AU  - Tamaian, Radu
AU  - Mercier, Sylvie
AU  - Boby, Celine
AU  - Monfoulet, Laurent-Emanuel
AU  - Glibetić, Marija
AU  - Morand, Christine
AU  - Milenković, Dragan
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/838
AB  - Cardioprotective effects of dietary anthocyanins are partly attributed to their ability to maintain endothelial function. However, the underlying cellular and molecular mechanisms of action are not fully understood. This study aimed to evaluate the effect of anthocyanins and their gut metabolites, at physiologically-relevant conditions, on endothelial cell (EC) function and decipher the underlying molecular mechanisms of action using integrated omics approaches. Primary EC were treated with a mixture of 0.1 mu M cyanidin-3-arabinoside, 0.1 mu M cyanidin-3-galactoside, 0.1 mu M cyanidin-3-glucoside, 0.1 mu M delphinidin-3-glucoside, 0.1 mu M peonidin-3-glucoside and 0.5 mu M 4-hydroxybenzaldehyde for 3 h or a mixture of gut metabolites: 0.2 mu M protocatechuic, 2 mu M vanillic, 1 mu M ferulic and 2 mu M hippuric acids for 18 h. Also, successive exposure of EC to both mixtures was performed to mimic anthocyanin pharmacokinetics following their intake. Inflammatory stress was induced using TNF alpha and monocytes added to assess adhesion and transmigration. Effects of these mixtures on gene, miRNA expression and their potential interaction with cell signalling were investigated. Anthocyanins and their gut metabolites significantly reduced monocyte adhesion and transendothelial migration. Gene expression analysis, using macroarrays, showed that tested compounds modulated the expression of genes involved in cell-cell adhesion, cytoskeleton organisation or focal adhesion. Bioinformatics analyses of gene expression data identified potential transcription factors involved in the observed nutrigenomic effects and signalling proteins regulating their activity. Molecular docking revealed cell signalling proteins to which these bioactives may bind to and potentially affect their activity and the activation of downstream signalling, effects that were in agreement with the results of Western blot analyses. Microarray analysis showed that anthocyanins and their gut metabolites affected miRNA expression in EC, especially those involved in regulation of EC permeability, contributing to the observed changes in EC function. Integration of these results revealed endothelial-protective properties of anthocyanins and their gut metabolites and deciphered new underlying multi-target and multi-layered mode of action.
PB  - Elsevier Science Inc, New York
T2  - Free Radical Biology & Medicine
T1  - Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability
EP  - 379
SP  - 364
VL  - 124
DO  - 10.1016/j.freeradbiomed.2018.06.027
ER  - 

@article{
author = "Krga, Irena and Tamaian, Radu and Mercier, Sylvie and Boby, Celine and Monfoulet, Laurent-Emanuel and Glibetić, Marija and Morand, Christine and Milenković, Dragan",
year = "2018",
abstract = "Cardioprotective effects of dietary anthocyanins are partly attributed to their ability to maintain endothelial function. However, the underlying cellular and molecular mechanisms of action are not fully understood. This study aimed to evaluate the effect of anthocyanins and their gut metabolites, at physiologically-relevant conditions, on endothelial cell (EC) function and decipher the underlying molecular mechanisms of action using integrated omics approaches. Primary EC were treated with a mixture of 0.1 mu M cyanidin-3-arabinoside, 0.1 mu M cyanidin-3-galactoside, 0.1 mu M cyanidin-3-glucoside, 0.1 mu M delphinidin-3-glucoside, 0.1 mu M peonidin-3-glucoside and 0.5 mu M 4-hydroxybenzaldehyde for 3 h or a mixture of gut metabolites: 0.2 mu M protocatechuic, 2 mu M vanillic, 1 mu M ferulic and 2 mu M hippuric acids for 18 h. Also, successive exposure of EC to both mixtures was performed to mimic anthocyanin pharmacokinetics following their intake. Inflammatory stress was induced using TNF alpha and monocytes added to assess adhesion and transmigration. Effects of these mixtures on gene, miRNA expression and their potential interaction with cell signalling were investigated. Anthocyanins and their gut metabolites significantly reduced monocyte adhesion and transendothelial migration. Gene expression analysis, using macroarrays, showed that tested compounds modulated the expression of genes involved in cell-cell adhesion, cytoskeleton organisation or focal adhesion. Bioinformatics analyses of gene expression data identified potential transcription factors involved in the observed nutrigenomic effects and signalling proteins regulating their activity. Molecular docking revealed cell signalling proteins to which these bioactives may bind to and potentially affect their activity and the activation of downstream signalling, effects that were in agreement with the results of Western blot analyses. Microarray analysis showed that anthocyanins and their gut metabolites affected miRNA expression in EC, especially those involved in regulation of EC permeability, contributing to the observed changes in EC function. Integration of these results revealed endothelial-protective properties of anthocyanins and their gut metabolites and deciphered new underlying multi-target and multi-layered mode of action.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology & Medicine",
title = "Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability",
pages = "379-364",
volume = "124",
doi = "10.1016/j.freeradbiomed.2018.06.027"
}

Krga, I., Tamaian, R., Mercier, S., Boby, C., Monfoulet, L., Glibetić, M., Morand, C.,& Milenković, D.. (2018). Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability. in Free Radical Biology & Medicine
Elsevier Science Inc, New York., 124, 364-379.
https://doi.org/10.1016/j.freeradbiomed.2018.06.027

Krga I, Tamaian R, Mercier S, Boby C, Monfoulet L, Glibetić M, Morand C, Milenković D. Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability. in Free Radical Biology & Medicine. 2018;124:364-379.
doi:10.1016/j.freeradbiomed.2018.06.027 .

Krga, Irena, Tamaian, Radu, Mercier, Sylvie, Boby, Celine, Monfoulet, Laurent-Emanuel, Glibetić, Marija, Morand, Christine, Milenković, Dragan, "Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability" in Free Radical Biology & Medicine, 124 (2018):364-379,
https://doi.org/10.1016/j.freeradbiomed.2018.06.027 . .

TY  - THES
AU  - Krga, Irena
PY  - 2018
UR  - http://nardus.mpn.gov.rs/handle/123456789/10767
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6606
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:19403/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50912015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1115
AB  - Increasing number of scientific evidence suggests the beneficial role of dietary anthocyanins, phytochemicals mainly present in berries and derived products, in cardiovascular health. These anthocyanin health benefits may be attributed to their effect on endothelial cells or platelets that represent the key players in the development of cardiovascular diseases (CVD). However, the exact molecular mechanisms underlying anthocyanin cardioprotective effects are not fully understood. The aim of this thesis was to investigate the effect of anthocyanins and their metabolites in vitro on endothelial and platelet function and identify the underlying mechanisms of their action using physiologically relevant conditions. Results from this thesis showed that the pretreatment of endothelial cells with physiologically relevant concentrations of circulating anthocyanins and their metabolites attenuated monocyte adhesion to activated endothelial cells as well as their transendothelial migration, which are the initial steps in the development of atherosclerosis that precede CVD. In agreement with these results, gene expression analysis revealed that the treatment of endothelial cells with these compounds modulated the expression of genes involved in regulation of cell-cell adhesion, actin cytoskeleton reorganisation, focal adhesion and leukocyte transmigration. Bioinformatics analyses of gene expression data allowed the identification of potential transcription factors involved in the observed nutrigenomic effects and cell signalling proteins regulating their activity. Molecular docking analyses further revealed cell signalling proteins to which these bioactives may bind to and potentially affect their activity and the activation of downstream signalling proteins and transcription factors, effects that were in agreement with the results of Western blot analyses. Anthocyanins and their metabolites also modulated the expression of microRNAs, especially those involved in regulation of endothelial cell permeability, contributing to the observed changes in endothelial cell function...
AB  - Kardiovaskularne bolesti predstavljaju oboljenja koja zahvataju srce i krvne sudove i najčešći su uzrok obolevanja i umiranja u svetu. Prema mestu javljanja mogu se podeliti na bolesti srca, cerebrovaskularne bolesti i bolesti perifernih krvnih sudova. Osnovni uzročnik kardiovaskularnih bolesti je ateroskleroza, hronično zapaljensko oboljenje velikih i srednje velikih arterijskih krvnih sudova koje se karakteriše aterosklerotičnim lezijama koje nastaju nakupljanjem lipida, ćelija i proteina vezivnog tkiva unutar zida krvnog suda. Ove patološke promene krvnih sudova ometaju normalan protok krvi, a u kasnijim stadijumima bolesti mogu dovesti i do pucanja plaka i aterotromboze, što za posledicu može imati infarkt miokarda ili šlog. Sam nastanak ateroskleroze povezan je sa poremećenom funkcijom endotela krvnog suda koja podstiče adheziju leukocita za aktivirane endotelne ćelije i njihovu transendotelnu migraciju. Unutar zida krvnog suda monociti se diferenciraju u makrofage, vrše ingestiju oksidovanog LDL holesterola i formiraju penušave ćelije čije nakupljanje vremenom dovodi do formiranja aterosklerotičnih lezija. Važnu ulogu u nastanku ateroskleroze imaju i trombociti. Interakcija aktiviranih trombocita sa leukocitima i endotelnim ćelijama podstiče adheziju leukocita i njihovu transendotelnu migraciju i time dodatno podstiče zapaljenski proces i razvoj ateroskleroze. Stoga, ispitivanje uzajamnog delovanja endotelnih ćelija, leukocita i trombocita predstavlja značajno područje istraživanja u cilju prevencije i kontrole kardiovaskularnih bolesti. Ishrana ima važnu ulogu, kako u nastanku i razvoju kardiovaskularnih bolest, tako i u njihovoj prevenciji i lečenju. Rezultati brojnih epidemioloških i kliničkih studija pokazuju povoljno delovanje ishrane bogate voćem i povrćem na kardiovaskularno zdravlje. Ovo pozitivno delovanje namirnica biljnog porekla pripisuje se delom njihovom niskom energetskom vrednošću, visokom sadržaju vlakana i esencijalnih mikronutrijenata, ali pre svega nenutritivnim, biološki aktivnim sastojcima, poput polifenola...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Effects of anthocyanins and their metabolites on the function of human endothelial cells and platelets in vitro
T1  - Uticaj antocijana i njihovih metabolita na funkciju endotelnih ćelija i trombocita čoveka in vitro
UR  - https://hdl.handle.net/21.15107/rcub_nardus_10767
ER  - 

@phdthesis{
author = "Krga, Irena",
year = "2018",
abstract = "Increasing number of scientific evidence suggests the beneficial role of dietary anthocyanins, phytochemicals mainly present in berries and derived products, in cardiovascular health. These anthocyanin health benefits may be attributed to their effect on endothelial cells or platelets that represent the key players in the development of cardiovascular diseases (CVD). However, the exact molecular mechanisms underlying anthocyanin cardioprotective effects are not fully understood. The aim of this thesis was to investigate the effect of anthocyanins and their metabolites in vitro on endothelial and platelet function and identify the underlying mechanisms of their action using physiologically relevant conditions. Results from this thesis showed that the pretreatment of endothelial cells with physiologically relevant concentrations of circulating anthocyanins and their metabolites attenuated monocyte adhesion to activated endothelial cells as well as their transendothelial migration, which are the initial steps in the development of atherosclerosis that precede CVD. In agreement with these results, gene expression analysis revealed that the treatment of endothelial cells with these compounds modulated the expression of genes involved in regulation of cell-cell adhesion, actin cytoskeleton reorganisation, focal adhesion and leukocyte transmigration. Bioinformatics analyses of gene expression data allowed the identification of potential transcription factors involved in the observed nutrigenomic effects and cell signalling proteins regulating their activity. Molecular docking analyses further revealed cell signalling proteins to which these bioactives may bind to and potentially affect their activity and the activation of downstream signalling proteins and transcription factors, effects that were in agreement with the results of Western blot analyses. Anthocyanins and their metabolites also modulated the expression of microRNAs, especially those involved in regulation of endothelial cell permeability, contributing to the observed changes in endothelial cell function..., Kardiovaskularne bolesti predstavljaju oboljenja koja zahvataju srce i krvne sudove i najčešći su uzrok obolevanja i umiranja u svetu. Prema mestu javljanja mogu se podeliti na bolesti srca, cerebrovaskularne bolesti i bolesti perifernih krvnih sudova. Osnovni uzročnik kardiovaskularnih bolesti je ateroskleroza, hronično zapaljensko oboljenje velikih i srednje velikih arterijskih krvnih sudova koje se karakteriše aterosklerotičnim lezijama koje nastaju nakupljanjem lipida, ćelija i proteina vezivnog tkiva unutar zida krvnog suda. Ove patološke promene krvnih sudova ometaju normalan protok krvi, a u kasnijim stadijumima bolesti mogu dovesti i do pucanja plaka i aterotromboze, što za posledicu može imati infarkt miokarda ili šlog. Sam nastanak ateroskleroze povezan je sa poremećenom funkcijom endotela krvnog suda koja podstiče adheziju leukocita za aktivirane endotelne ćelije i njihovu transendotelnu migraciju. Unutar zida krvnog suda monociti se diferenciraju u makrofage, vrše ingestiju oksidovanog LDL holesterola i formiraju penušave ćelije čije nakupljanje vremenom dovodi do formiranja aterosklerotičnih lezija. Važnu ulogu u nastanku ateroskleroze imaju i trombociti. Interakcija aktiviranih trombocita sa leukocitima i endotelnim ćelijama podstiče adheziju leukocita i njihovu transendotelnu migraciju i time dodatno podstiče zapaljenski proces i razvoj ateroskleroze. Stoga, ispitivanje uzajamnog delovanja endotelnih ćelija, leukocita i trombocita predstavlja značajno područje istraživanja u cilju prevencije i kontrole kardiovaskularnih bolesti. Ishrana ima važnu ulogu, kako u nastanku i razvoju kardiovaskularnih bolest, tako i u njihovoj prevenciji i lečenju. Rezultati brojnih epidemioloških i kliničkih studija pokazuju povoljno delovanje ishrane bogate voćem i povrćem na kardiovaskularno zdravlje. Ovo pozitivno delovanje namirnica biljnog porekla pripisuje se delom njihovom niskom energetskom vrednošću, visokom sadržaju vlakana i esencijalnih mikronutrijenata, ali pre svega nenutritivnim, biološki aktivnim sastojcima, poput polifenola...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Effects of anthocyanins and their metabolites on the function of human endothelial cells and platelets in vitro, Uticaj antocijana i njihovih metabolita na funkciju endotelnih ćelija i trombocita čoveka in vitro",
url = "https://hdl.handle.net/21.15107/rcub_nardus_10767"
}

Krga, I.. (2018). Effects of anthocyanins and their metabolites on the function of human endothelial cells and platelets in vitro. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_10767

Krga I. Effects of anthocyanins and their metabolites on the function of human endothelial cells and platelets in vitro. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_10767 .

Krga, Irena, "Effects of anthocyanins and their metabolites on the function of human endothelial cells and platelets in vitro" (2018),
https://hdl.handle.net/21.15107/rcub_nardus_10767 .

TY  - JOUR
AU  - Krga, Irena
AU  - Milenković, Dragan
AU  - Morand, Christine
AU  - Monfoulet, Laurent-Emanuel
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/702
AB  - Polyphenols are plant food microconstituents that are widely distributed in the human diet, with fruits and fruit-derived products as one of the main dietary sources. Epidemiological studies have shown an inverse relationship between the intake of different classes of polyphenols and the risk of myocardial infarction or cardiovascular disease (CVD) mortality. These compounds have been associated with the promotion of cardiovascular health as evidenced by clinical studies reporting beneficial effects of polyphenol-rich fruit consumption on intermediate markers of cardiovascular diseases. Additionally, animal and in vitro studies have indicated positive roles of polyphenols in preventing dysfunctions associated with the development of cardiovascular diseases. However, the mechanisms of action underlying their beneficial effects appear complex and are not fully understood. This review aims to provide an update on the nutrigenomic effects of different groups of polyphenols from fruits and especially focuses on their cardiovascular protective effects in cell and animal studies.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols
EP  - 3676
IS  - 9
SP  - 3656
VL  - 7
DO  - 10.1039/c6fo00596a
ER  - 

@article{
author = "Krga, Irena and Milenković, Dragan and Morand, Christine and Monfoulet, Laurent-Emanuel",
year = "2016",
abstract = "Polyphenols are plant food microconstituents that are widely distributed in the human diet, with fruits and fruit-derived products as one of the main dietary sources. Epidemiological studies have shown an inverse relationship between the intake of different classes of polyphenols and the risk of myocardial infarction or cardiovascular disease (CVD) mortality. These compounds have been associated with the promotion of cardiovascular health as evidenced by clinical studies reporting beneficial effects of polyphenol-rich fruit consumption on intermediate markers of cardiovascular diseases. Additionally, animal and in vitro studies have indicated positive roles of polyphenols in preventing dysfunctions associated with the development of cardiovascular diseases. However, the mechanisms of action underlying their beneficial effects appear complex and are not fully understood. This review aims to provide an update on the nutrigenomic effects of different groups of polyphenols from fruits and especially focuses on their cardiovascular protective effects in cell and animal studies.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols",
pages = "3676-3656",
number = "9",
volume = "7",
doi = "10.1039/c6fo00596a"
}

Krga, I., Milenković, D., Morand, C.,& Monfoulet, L.. (2016). An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols. in Food & Function
Royal Soc Chemistry, Cambridge., 7(9), 3656-3676.
https://doi.org/10.1039/c6fo00596a

Krga I, Milenković D, Morand C, Monfoulet L. An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols. in Food & Function. 2016;7(9):3656-3676.
doi:10.1039/c6fo00596a .

Krga, Irena, Milenković, Dragan, Morand, Christine, Monfoulet, Laurent-Emanuel, "An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols" in Food & Function, 7, no. 9 (2016):3656-3676,
https://doi.org/10.1039/c6fo00596a . .

TY  - JOUR
AU  - Krga, Irena
AU  - Monfoulet, Laurent-Emanuel
AU  - Konić-Ristić, Aleksandra
AU  - Mercier, Sylvie
AU  - Glibetić, Marija
AU  - Morand, Christine
AU  - Milenković, Dragan
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/718
AB  - An increasing number of evidence suggests a protective role of dietary anthocyanins against cardiovascular diseases. Anthocyanins' extensive metabolism indicates that their metabolites could be responsible for the protective effects associated with consumption of anthocyanin-rich foods. The aim of this work was to investigate the effect of plasma anthocyanins and their metabolites on the adhesion of monocytes to TNF alpha-activated endothelial cells and on the expression of genes encoding cell adhesion molecules. Human umbilical vein endothelial cells (HUVECs) were exposed to circulating anthocyanins: cyanidin-3-arabinoside, cyanidin-3-galactoside, cyanidin-3-glucoside, delphinidin-3-glucoside, peonidin-3-glucoside, anthocyanin degradation product: 4-hydroxybenzaidehyde, or to their gut metabolites: protocatechuic, vanillic, ferulic and hippuric acid, at physiologically-relevant concentrations (0.1-2 mu M) and time of exposure. Both anthocyanins and gut metabolites decreased the adhesion of monocytes to HUVECs, with a magnitude ranging from 18.1% to 47%. The mixture of anthocyanins and that of gut metabolites also reduced monocyte adhesion. However, no significant effect on the expression of genes encoding E-selectin, ICAM1 and VCAM1 was observed, suggesting that other molecular targets are involved in the observed effect. In conclusion, this study showed the potency of anthocyanins and their gut metabolites to modulate the adhesion of monocytes to endothelial cells, the initial step in atherosclerosis development, under physiologically-relevant conditions.
PB  - Elsevier Science Inc, New York
T2  - Archives of Biochemistry & Biophysics
T1  - Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations
EP  - 59
SP  - 51
VL  - 599
DO  - 10.1016/j.abb.2016.02.006
ER  - 

@article{
author = "Krga, Irena and Monfoulet, Laurent-Emanuel and Konić-Ristić, Aleksandra and Mercier, Sylvie and Glibetić, Marija and Morand, Christine and Milenković, Dragan",
year = "2016",
abstract = "An increasing number of evidence suggests a protective role of dietary anthocyanins against cardiovascular diseases. Anthocyanins' extensive metabolism indicates that their metabolites could be responsible for the protective effects associated with consumption of anthocyanin-rich foods. The aim of this work was to investigate the effect of plasma anthocyanins and their metabolites on the adhesion of monocytes to TNF alpha-activated endothelial cells and on the expression of genes encoding cell adhesion molecules. Human umbilical vein endothelial cells (HUVECs) were exposed to circulating anthocyanins: cyanidin-3-arabinoside, cyanidin-3-galactoside, cyanidin-3-glucoside, delphinidin-3-glucoside, peonidin-3-glucoside, anthocyanin degradation product: 4-hydroxybenzaidehyde, or to their gut metabolites: protocatechuic, vanillic, ferulic and hippuric acid, at physiologically-relevant concentrations (0.1-2 mu M) and time of exposure. Both anthocyanins and gut metabolites decreased the adhesion of monocytes to HUVECs, with a magnitude ranging from 18.1% to 47%. The mixture of anthocyanins and that of gut metabolites also reduced monocyte adhesion. However, no significant effect on the expression of genes encoding E-selectin, ICAM1 and VCAM1 was observed, suggesting that other molecular targets are involved in the observed effect. In conclusion, this study showed the potency of anthocyanins and their gut metabolites to modulate the adhesion of monocytes to endothelial cells, the initial step in atherosclerosis development, under physiologically-relevant conditions.",
publisher = "Elsevier Science Inc, New York",
journal = "Archives of Biochemistry & Biophysics",
title = "Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations",
pages = "59-51",
volume = "599",
doi = "10.1016/j.abb.2016.02.006"
}

Krga, I., Monfoulet, L., Konić-Ristić, A., Mercier, S., Glibetić, M., Morand, C.,& Milenković, D.. (2016). Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations. in Archives of Biochemistry & Biophysics
Elsevier Science Inc, New York., 599, 51-59.
https://doi.org/10.1016/j.abb.2016.02.006

Krga I, Monfoulet L, Konić-Ristić A, Mercier S, Glibetić M, Morand C, Milenković D. Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations. in Archives of Biochemistry & Biophysics. 2016;599:51-59.
doi:10.1016/j.abb.2016.02.006 .

Krga, Irena, Monfoulet, Laurent-Emanuel, Konić-Ristić, Aleksandra, Mercier, Sylvie, Glibetić, Marija, Morand, Christine, Milenković, Dragan, "Anthocyanins and their gut metabolites reduce the adhesion of monocyte to TNF alpha-activated endothelial cells at physiologically relevant concentrations" in Archives of Biochemistry & Biophysics, 599 (2016):51-59,
https://doi.org/10.1016/j.abb.2016.02.006 . .