TY  - JOUR
AU  - Pokimica, Biljana
AU  - Garcia-Conesa, Maria-Teresa
AU  - Zec, Manja M.
AU  - Debeljak-Martačić, Jasmina
AU  - Ranković, Slavica G.
AU  - Vidović, Nevena Đ.
AU  - Petrović-Oggiano, Gordana P.
AU  - Konić-Ristić, Aleksandra
AU  - Glibetić, Marija
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/977
AB  - Chokeberry polyphenols have been suggested to reduce cholesterol and blood pressure and thus protect against cardiovascular diseases (CVD), but the evidence in humans is limited and inconsistent. This randomized double-blinded three-parallel groups trial investigated the changes in various anthropometric and clinical biomarkers, and in plasma phospholipids fatty acids (PPFA) in volunteers at cardiovascular risk after a four-week intervention with 100 mL/day of (1) chokeberry juice with a high-dose of polyphenols (1177.11 mg gallic acid equivalents, GAE); (2) chokeberry juice with a low-dose of polyphenols (294.28 mg GAE) and; (3) a nutritionally matched polyphenol-free placebo drink. Our results indicate that the intake of chokeberry juice containing either the low or the high dose of polyphenols cannot be linked with a reduction in total- and low-density lipoprotein (LDL)cholesterol or in systolic (SBP) and diastolic (DBP) blood pressure in comparison with the consumption of the placebo drink. However, we found evidence of moderate changes in the PPFA, i.e., increased saturated fatty acids (SFA), mostly palmitic acid, and reduced n-6 polyunsaturated fatty acids (PUFA), principally linoleic acid (LA) with the intake of chokeberry against the placebo. These effects may be associated with the polyphenols but we could not differentiate a clear dose-response effect. Further research is still needed to elucidate the contribution of the polyphenolic fraction to the potential cardiovascular effects of the chokeberry and to build up the evidence of its potential benefit via the modulation of PPFA composition.
PB  - MDPI, Basel
T2  - Nutrients
T1  - Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk
IS  - 4
VL  - 11
DO  - 10.3390/nu11040850
ER  - 

@article{
author = "Pokimica, Biljana and Garcia-Conesa, Maria-Teresa and Zec, Manja M. and Debeljak-Martačić, Jasmina and Ranković, Slavica G. and Vidović, Nevena Đ. and Petrović-Oggiano, Gordana P. and Konić-Ristić, Aleksandra and Glibetić, Marija",
year = "2019",
abstract = "Chokeberry polyphenols have been suggested to reduce cholesterol and blood pressure and thus protect against cardiovascular diseases (CVD), but the evidence in humans is limited and inconsistent. This randomized double-blinded three-parallel groups trial investigated the changes in various anthropometric and clinical biomarkers, and in plasma phospholipids fatty acids (PPFA) in volunteers at cardiovascular risk after a four-week intervention with 100 mL/day of (1) chokeberry juice with a high-dose of polyphenols (1177.11 mg gallic acid equivalents, GAE); (2) chokeberry juice with a low-dose of polyphenols (294.28 mg GAE) and; (3) a nutritionally matched polyphenol-free placebo drink. Our results indicate that the intake of chokeberry juice containing either the low or the high dose of polyphenols cannot be linked with a reduction in total- and low-density lipoprotein (LDL)cholesterol or in systolic (SBP) and diastolic (DBP) blood pressure in comparison with the consumption of the placebo drink. However, we found evidence of moderate changes in the PPFA, i.e., increased saturated fatty acids (SFA), mostly palmitic acid, and reduced n-6 polyunsaturated fatty acids (PUFA), principally linoleic acid (LA) with the intake of chokeberry against the placebo. These effects may be associated with the polyphenols but we could not differentiate a clear dose-response effect. Further research is still needed to elucidate the contribution of the polyphenolic fraction to the potential cardiovascular effects of the chokeberry and to build up the evidence of its potential benefit via the modulation of PPFA composition.",
publisher = "MDPI, Basel",
journal = "Nutrients",
title = "Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk",
number = "4",
volume = "11",
doi = "10.3390/nu11040850"
}

Pokimica, B., Garcia-Conesa, M., Zec, M. M., Debeljak-Martačić, J., Ranković, S. G., Vidović, N. Đ., Petrović-Oggiano, G. P., Konić-Ristić, A.,& Glibetić, M.. (2019). Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk. in Nutrients
MDPI, Basel., 11(4).
https://doi.org/10.3390/nu11040850

Pokimica B, Garcia-Conesa M, Zec MM, Debeljak-Martačić J, Ranković SG, Vidović NĐ, Petrović-Oggiano GP, Konić-Ristić A, Glibetić M. Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk. in Nutrients. 2019;11(4).
doi:10.3390/nu11040850 .

Pokimica, Biljana, Garcia-Conesa, Maria-Teresa, Zec, Manja M., Debeljak-Martačić, Jasmina, Ranković, Slavica G., Vidović, Nevena Đ., Petrović-Oggiano, Gordana P., Konić-Ristić, Aleksandra, Glibetić, Marija, "Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk" in Nutrients, 11, no. 4 (2019),
https://doi.org/10.3390/nu11040850 . .

TY  - JOUR
AU  - Pokimica, Biljana
AU  - Garcia-Conesa, Maria-Teresa
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/861
AB  - Pre-clinical cell and animal nutrigenomic studies have long suggested the modulation of the transcription of multiple gene targets in cells and tissues as a potential molecular mechanism of action underlying the beneficial effects attributed to plant-derived bioactive compounds. To try to demonstrate these molecular effects in humans, a considerable number of clinical trials have now explored the changes in the expression levels of selected genes in various human cell and tissue samples following intervention with different dietary sources of bioactive compounds. In this review, we have compiled a total of 75 human studies exploring gene expression changes using quantitative reverse transcription PCR (RT-qPCR). We have critically appraised the study design and methodology used as well as the gene expression results reported. We herein pinpoint some of the main drawbacks and gaps in the experimental strategies applied, as well as the high interindividual variability of the results and the limited evidence supporting some of the investigated genes as potential responsive targets. We reinforce the need to apply normalized procedures and follow well-established methodological guidelines in future studies in order to achieve improved and reliable results that would allow for more relevant and biologically meaningful results.
PB  - MDPI, Basel
T2  - Nutrients
T1  - Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies
IS  - 7
VL  - 10
DO  - 10.3390/nu10070807
ER  - 

@article{
author = "Pokimica, Biljana and Garcia-Conesa, Maria-Teresa",
year = "2018",
abstract = "Pre-clinical cell and animal nutrigenomic studies have long suggested the modulation of the transcription of multiple gene targets in cells and tissues as a potential molecular mechanism of action underlying the beneficial effects attributed to plant-derived bioactive compounds. To try to demonstrate these molecular effects in humans, a considerable number of clinical trials have now explored the changes in the expression levels of selected genes in various human cell and tissue samples following intervention with different dietary sources of bioactive compounds. In this review, we have compiled a total of 75 human studies exploring gene expression changes using quantitative reverse transcription PCR (RT-qPCR). We have critically appraised the study design and methodology used as well as the gene expression results reported. We herein pinpoint some of the main drawbacks and gaps in the experimental strategies applied, as well as the high interindividual variability of the results and the limited evidence supporting some of the investigated genes as potential responsive targets. We reinforce the need to apply normalized procedures and follow well-established methodological guidelines in future studies in order to achieve improved and reliable results that would allow for more relevant and biologically meaningful results.",
publisher = "MDPI, Basel",
journal = "Nutrients",
title = "Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies",
number = "7",
volume = "10",
doi = "10.3390/nu10070807"
}

Pokimica, B.,& Garcia-Conesa, M.. (2018). Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies. in Nutrients
MDPI, Basel., 10(7).
https://doi.org/10.3390/nu10070807

Pokimica B, Garcia-Conesa M. Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies. in Nutrients. 2018;10(7).
doi:10.3390/nu10070807 .

Pokimica, Biljana, Garcia-Conesa, Maria-Teresa, "Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies" in Nutrients, 10, no. 7 (2018),
https://doi.org/10.3390/nu10070807 . .