TY  - JOUR
AU  - Bošković, Maja
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Zec, Manja
AU  - Debeljak-Martačić, Jasmina
AU  - Stanković, Aleksandra
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1273
AB  - Increased fructose consumption has been linked with chronic inflammation and metabolic syndrome (MetS). Activation of the renin-angiotensin system (RAS) and NF-κB have been detected in MetS. Walnuts are a rich source of polyunsaturated omega-3 fatty acids (n-3 PUFA) that were suggested to exert anti-inflammatory effects related to cardio-metabolic health. We hypothesized that walnut supplementation has the capacity to revert unfavorable fructose-rich diet (FRD)-induced activation of cardiac RAS and NF-κB in male rats. Due to the lack of similar studies, we investigated the effects of walnut supplementation (6 weeks) on the expression of four RAS molecules (ACE, ACE2, AT1R, and AT2R) and NF-κB in rat heart after FRD (10% w/v, 9 weeks). In addition, we followed the changes in the n-6/n-3 PUFA ratio in the total pool of heart lipids after both treatments to elucidate the walnut effects on fatty acids in the heart. 36 animals (9 per group) participated in the experiment. FRD significantly increased the ACE protein level in the heart (p < 0.001). Walnut supplementation significantly increased the ACE2 protein level in the heart of FRD (p < 0.001). In addition, walnut supplementation showed a significant main effect on the arachidonic acid/eicosapentaenoic acid ratio (p = 0.004). Walnut supplementation significantly reduced this ratio, in comparison with both, the control group (C vs. FW, p < 0.05) and the FRD group (F vs. FW, p < 0.05). However, walnut treatment failed to revert the significant effect of fructose (p < 0.001) on the elevation of NF-κB protein level. Our results suggest a beneficial effect of walnut supplementation on ACE2 protein level and n-6/n-3 PUFA level in the heart of the animal model of MetS. Such results highlight the approach of omega-3-rich walnut supplementation in the stimulation of endogenous production of favorable molecules in the heart which could be an affordable nutritional treatment formaintenance of cardio-metabolic health.
PB  - Frontiers Media S.A.
T2  - Frontiers in Physiology
T1  - Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart
SP  - 942459
VL  - 13
DO  - 10.3389/fphys.2022.942459
ER  - 

@article{
author = "Bošković, Maja and Živković, Maja and Korićanac, Goran and Tepavčević, Snežana and Zec, Manja and Debeljak-Martačić, Jasmina and Stanković, Aleksandra",
year = "2022",
abstract = "Increased fructose consumption has been linked with chronic inflammation and metabolic syndrome (MetS). Activation of the renin-angiotensin system (RAS) and NF-κB have been detected in MetS. Walnuts are a rich source of polyunsaturated omega-3 fatty acids (n-3 PUFA) that were suggested to exert anti-inflammatory effects related to cardio-metabolic health. We hypothesized that walnut supplementation has the capacity to revert unfavorable fructose-rich diet (FRD)-induced activation of cardiac RAS and NF-κB in male rats. Due to the lack of similar studies, we investigated the effects of walnut supplementation (6 weeks) on the expression of four RAS molecules (ACE, ACE2, AT1R, and AT2R) and NF-κB in rat heart after FRD (10% w/v, 9 weeks). In addition, we followed the changes in the n-6/n-3 PUFA ratio in the total pool of heart lipids after both treatments to elucidate the walnut effects on fatty acids in the heart. 36 animals (9 per group) participated in the experiment. FRD significantly increased the ACE protein level in the heart (p < 0.001). Walnut supplementation significantly increased the ACE2 protein level in the heart of FRD (p < 0.001). In addition, walnut supplementation showed a significant main effect on the arachidonic acid/eicosapentaenoic acid ratio (p = 0.004). Walnut supplementation significantly reduced this ratio, in comparison with both, the control group (C vs. FW, p < 0.05) and the FRD group (F vs. FW, p < 0.05). However, walnut treatment failed to revert the significant effect of fructose (p < 0.001) on the elevation of NF-κB protein level. Our results suggest a beneficial effect of walnut supplementation on ACE2 protein level and n-6/n-3 PUFA level in the heart of the animal model of MetS. Such results highlight the approach of omega-3-rich walnut supplementation in the stimulation of endogenous production of favorable molecules in the heart which could be an affordable nutritional treatment formaintenance of cardio-metabolic health.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Physiology",
title = "Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart",
pages = "942459",
volume = "13",
doi = "10.3389/fphys.2022.942459"
}

Bošković, M., Živković, M., Korićanac, G., Tepavčević, S., Zec, M., Debeljak-Martačić, J.,& Stanković, A.. (2022). Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart. in Frontiers in Physiology
Frontiers Media S.A.., 13, 942459.
https://doi.org/10.3389/fphys.2022.942459

Bošković M, Živković M, Korićanac G, Tepavčević S, Zec M, Debeljak-Martačić J, Stanković A. Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart. in Frontiers in Physiology. 2022;13:942459.
doi:10.3389/fphys.2022.942459 .

Bošković, Maja, Živković, Maja, Korićanac, Goran, Tepavčević, Snežana, Zec, Manja, Debeljak-Martačić, Jasmina, Stanković, Aleksandra, "Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart" in Frontiers in Physiology, 13 (2022):942459,
https://doi.org/10.3389/fphys.2022.942459 . .

TY  - CONF
AU  - Krga, Irena
AU  - Zec, Manja
AU  - Takić, Marija
AU  - Debeljak-Martačić, Jasmina
AU  - Korićanac, Goran
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Glibetić, Marija
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1384
PB  - Belgrade : University of Belgrade
C3  - UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts
T1  - Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1384
ER  - 

@conference{
author = "Krga, Irena and Zec, Manja and Takić, Marija and Debeljak-Martačić, Jasmina and Korićanac, Goran and Ranković, Slavica and Popović, Tamara and Glibetić, Marija",
year = "2021",
publisher = "Belgrade : University of Belgrade",
journal = "UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts",
title = "Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1384"
}

Krga, I., Zec, M., Takić, M., Debeljak-Martačić, J., Korićanac, G., Ranković, S., Popović, T.,& Glibetić, M.. (2021). Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats. in UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts
Belgrade : University of Belgrade..
https://hdl.handle.net/21.15107/rcub_rimi_1384

Krga I, Zec M, Takić M, Debeljak-Martačić J, Korićanac G, Ranković S, Popović T, Glibetić M. Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats. in UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_rimi_1384 .

Krga, Irena, Zec, Manja, Takić, Marija, Debeljak-Martačić, Jasmina, Korićanac, Goran, Ranković, Slavica, Popović, Tamara, Glibetić, Marija, "Walnuts consumption modulates endigenous metabolic conversion towards long-chain fatty acids and effects individual fatty acid content in plasma and liver of fructose-fed rats" in UniFood conference, 2nd International UniFood Conference Belgrade, 24 - 25 September 2021 - Book of Abstracts (2021),
https://hdl.handle.net/21.15107/rcub_rimi_1384 .

TY  - JOUR
AU  - Bošković, Maja
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanišić, Jelena
AU  - Zec, Manja
AU  - Krga, Irena
AU  - Stanković, Aleksandra
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1144
AB  - The benefits of walnut (Juglans regia) consumption for metabolic health are known, but the molecular background underlying their putative antioxidant and anti-inflammatory/immunomodulatory effects is underexplored. We assessed that walnut supplementation (6 weeks) reverted unfavorable changes of the SIRT1/FoxO3a/MnSOD/catalase axis in the heart induced by fructose-rich diet (FRD). Intriguingly, Nox4 was increased by both FRD and walnut supplementation. FRD increased the cytosolic fraction and decreased the nuclear fraction of the uniquely elucidated ChREBP in the heart. The ChREBP nuclear fraction was decreased in control rats subjected to walnuts. In addition, walnut consumption was associated with a reduction in systolic BP in FRD and a decrease in fatty acid AA/EPA and AA/DHA ratios in plasma. In summary, the protective effect of walnut supplementation was detected in male rats following the fructose-induced decrease in antioxidative/anti-inflammatory capacity of cardiac tissue and increase in plasma predictors of low-grade inflammation. The current results provide a novel insight into the relationship between nutrients, cellular energy homeostasis, and the modulators of inflammatory/immune response in metabolic syndrome, emphasizing the heart and highlighting a track for translation into nutrition and dietary therapeutic approaches against metabolic disease.
PB  - Hindawi
T2  - Oxidative Medicine and Cellular Longevity
T1  - Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet
SP  - e5543025
VL  - 2021
DO  - 10.1155/2021/5543025
ER  - 

@article{
author = "Bošković, Maja and Živković, Maja and Korićanac, Goran and Stanišić, Jelena and Zec, Manja and Krga, Irena and Stanković, Aleksandra",
year = "2021",
abstract = "The benefits of walnut (Juglans regia) consumption for metabolic health are known, but the molecular background underlying their putative antioxidant and anti-inflammatory/immunomodulatory effects is underexplored. We assessed that walnut supplementation (6 weeks) reverted unfavorable changes of the SIRT1/FoxO3a/MnSOD/catalase axis in the heart induced by fructose-rich diet (FRD). Intriguingly, Nox4 was increased by both FRD and walnut supplementation. FRD increased the cytosolic fraction and decreased the nuclear fraction of the uniquely elucidated ChREBP in the heart. The ChREBP nuclear fraction was decreased in control rats subjected to walnuts. In addition, walnut consumption was associated with a reduction in systolic BP in FRD and a decrease in fatty acid AA/EPA and AA/DHA ratios in plasma. In summary, the protective effect of walnut supplementation was detected in male rats following the fructose-induced decrease in antioxidative/anti-inflammatory capacity of cardiac tissue and increase in plasma predictors of low-grade inflammation. The current results provide a novel insight into the relationship between nutrients, cellular energy homeostasis, and the modulators of inflammatory/immune response in metabolic syndrome, emphasizing the heart and highlighting a track for translation into nutrition and dietary therapeutic approaches against metabolic disease.",
publisher = "Hindawi",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet",
pages = "e5543025",
volume = "2021",
doi = "10.1155/2021/5543025"
}

Bošković, M., Živković, M., Korićanac, G., Stanišić, J., Zec, M., Krga, I.,& Stanković, A.. (2021). Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet. in Oxidative Medicine and Cellular Longevity
Hindawi., 2021, e5543025.
https://doi.org/10.1155/2021/5543025

Bošković M, Živković M, Korićanac G, Stanišić J, Zec M, Krga I, Stanković A. Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet. in Oxidative Medicine and Cellular Longevity. 2021;2021:e5543025.
doi:10.1155/2021/5543025 .

Bošković, Maja, Živković, Maja, Korićanac, Goran, Stanišić, Jelena, Zec, Manja, Krga, Irena, Stanković, Aleksandra, "Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet" in Oxidative Medicine and Cellular Longevity, 2021 (2021):e5543025,
https://doi.org/10.1155/2021/5543025 . .

TY  - JOUR
AU  - Zec, Manja M.
AU  - Krga, Irena
AU  - Takić, Marija
AU  - Debeljak-Martačić, Jasmina
AU  - Korićanac, Goran
AU  - Ranković, Slavica G.
AU  - Popović, Tamara B.
AU  - Pantelić, Marija
AU  - Glibetić, Marija
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/984
AB  - Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats
EP  - 28145
IS  - 43
SP  - 28136
VL  - 5
DO  - 10.1021/acsomega.0c03784
ER  - 

@article{
author = "Zec, Manja M. and Krga, Irena and Takić, Marija and Debeljak-Martačić, Jasmina and Korićanac, Goran and Ranković, Slavica G. and Popović, Tamara B. and Pantelić, Marija and Glibetić, Marija",
year = "2020",
abstract = "Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats",
pages = "28145-28136",
number = "43",
volume = "5",
doi = "10.1021/acsomega.0c03784"
}

Zec, M. M., Krga, I., Takić, M., Debeljak-Martačić, J., Korićanac, G., Ranković, S. G., Popović, T. B., Pantelić, M.,& Glibetić, M.. (2020). Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega
Amer Chemical Soc, Washington., 5(43), 28136-28145.
https://doi.org/10.1021/acsomega.0c03784

Zec MM, Krga I, Takić M, Debeljak-Martačić J, Korićanac G, Ranković SG, Popović TB, Pantelić M, Glibetić M. Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega. 2020;5(43):28136-28145.
doi:10.1021/acsomega.0c03784 .

Zec, Manja M., Krga, Irena, Takić, Marija, Debeljak-Martačić, Jasmina, Korićanac, Goran, Ranković, Slavica G., Popović, Tamara B., Pantelić, Marija, Glibetić, Marija, "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats" in ACS Omega, 5, no. 43 (2020):28136-28145,
https://doi.org/10.1021/acsomega.0c03784 . .

TY  - JOUR
AU  - Stanisić, Jelena
AU  - Ivković, Tamara
AU  - Romić, Snježana
AU  - Zec, Manja M.
AU  - Culafić, Tijana
AU  - Stojiljković, Mojca
AU  - Korićanac, Goran
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/7
AB  - Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Food Sciences & Nutrition
T1  - Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2
DO  - 10.1080/09637486.2020.1796931
ER  - 

@article{
author = "Stanisić, Jelena and Ivković, Tamara and Romić, Snježana and Zec, Manja M. and Culafić, Tijana and Stojiljković, Mojca and Korićanac, Goran",
year = "2020",
abstract = "Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Food Sciences & Nutrition",
title = "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2",
doi = "10.1080/09637486.2020.1796931"
}

Stanisić, J., Ivković, T., Romić, S., Zec, M. M., Culafić, T., Stojiljković, M.,& Korićanac, G.. (2020). Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences & Nutrition
Taylor & Francis Ltd, Abingdon..
https://doi.org/10.1080/09637486.2020.1796931

Stanisić J, Ivković T, Romić S, Zec MM, Culafić T, Stojiljković M, Korićanac G. Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences & Nutrition. 2020;.
doi:10.1080/09637486.2020.1796931 .

Stanisić, Jelena, Ivković, Tamara, Romić, Snježana, Zec, Manja M., Culafić, Tijana, Stojiljković, Mojca, Korićanac, Goran, "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2" in International Journal of Food Sciences & Nutrition (2020),
https://doi.org/10.1080/09637486.2020.1796931 . .