TY  - JOUR
AU  - Penezić, Ana Z.
AU  - Aćimović, Jelena M.
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Takić, Marija
AU  - Mandić, Ljuba M.
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/946
AB  - Cys34 thiol group of human serum albumin (HSA) represents major plasma antioxidant. Its reactivity is influenced by multiple factors. The influence of fatty acids (FA; saturated, mono, and poly unsaturated acids from fish oil) binding to HSA, on copper(II) binding affinity and Cys34 thiol group accessibility/reactivity, in the presence of carbonylation agent (methylglyoxal, MG) was examined. HSA-copper(II) content, thiol group reactivity, and HSA carbonylation level were monitored spectrophotometrically. Changes in HSA were followed by fluorescence spectroscopy and native PAG electrophoresis. FA/HSA molar ratio was screened by GC. Together, binding of copper(II) ions and FA to HSA increase the reactivity of Cys34 thiol group (depending on the type of FA), with constant contribution of copper(II) ions of one-third. Carbonylation of FA-HSA-Cu(II) complexes caused a decrease in the Cys34 thiol group content, accompanied by a decrease in the content of HSA-bound copper. The carbonylation level of guanidine groups was not affected by FAs and copper(II) binding. Fluorescent emission spectra of FA-HSA-Cu(II)-MG complexes showed conformational changes in HSA molecule. Although binding of fatty acids and copper ions caused a significant increase in the thiol group reactivity, Cys34 thiol from FA-HSA-Cu(II) complexes reacted with MG in smaller extent than expected, probably as a consequence of conformational changes introduced by carbonylation. Increase in the percentage of reacted-free thiol groups with MG (due to FA and copper binding) may not seem to be very significant, but it is very important in complex biological systems, where catalytic metal is present. [GRAPHICS] .
PB  - Springer, New York
T2  - Journal of Biological Inorganic Chemistry
T1  - The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding
EP  - 70
IS  - 1
SP  - 61
VL  - 24
DO  - 10.1007/s00775-018-1628-7
ER  - 

@article{
author = "Penezić, Ana Z. and Aćimović, Jelena M. and Pavićević, Ivan D. and Jovanović, Vesna B. and Takić, Marija and Mandić, Ljuba M.",
year = "2019",
abstract = "Cys34 thiol group of human serum albumin (HSA) represents major plasma antioxidant. Its reactivity is influenced by multiple factors. The influence of fatty acids (FA; saturated, mono, and poly unsaturated acids from fish oil) binding to HSA, on copper(II) binding affinity and Cys34 thiol group accessibility/reactivity, in the presence of carbonylation agent (methylglyoxal, MG) was examined. HSA-copper(II) content, thiol group reactivity, and HSA carbonylation level were monitored spectrophotometrically. Changes in HSA were followed by fluorescence spectroscopy and native PAG electrophoresis. FA/HSA molar ratio was screened by GC. Together, binding of copper(II) ions and FA to HSA increase the reactivity of Cys34 thiol group (depending on the type of FA), with constant contribution of copper(II) ions of one-third. Carbonylation of FA-HSA-Cu(II) complexes caused a decrease in the Cys34 thiol group content, accompanied by a decrease in the content of HSA-bound copper. The carbonylation level of guanidine groups was not affected by FAs and copper(II) binding. Fluorescent emission spectra of FA-HSA-Cu(II)-MG complexes showed conformational changes in HSA molecule. Although binding of fatty acids and copper ions caused a significant increase in the thiol group reactivity, Cys34 thiol from FA-HSA-Cu(II) complexes reacted with MG in smaller extent than expected, probably as a consequence of conformational changes introduced by carbonylation. Increase in the percentage of reacted-free thiol groups with MG (due to FA and copper binding) may not seem to be very significant, but it is very important in complex biological systems, where catalytic metal is present. [GRAPHICS] .",
publisher = "Springer, New York",
journal = "Journal of Biological Inorganic Chemistry",
title = "The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding",
pages = "70-61",
number = "1",
volume = "24",
doi = "10.1007/s00775-018-1628-7"
}

Penezić, A. Z., Aćimović, J. M., Pavićević, I. D., Jovanović, V. B., Takić, M.,& Mandić, L. M.. (2019). The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding. in Journal of Biological Inorganic Chemistry
Springer, New York., 24(1), 61-70.
https://doi.org/10.1007/s00775-018-1628-7

Penezić AZ, Aćimović JM, Pavićević ID, Jovanović VB, Takić M, Mandić LM. The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding. in Journal of Biological Inorganic Chemistry. 2019;24(1):61-70.
doi:10.1007/s00775-018-1628-7 .

Penezić, Ana Z., Aćimović, Jelena M., Pavićević, Ivan D., Jovanović, Vesna B., Takić, Marija, Mandić, Ljuba M., "The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding" in Journal of Biological Inorganic Chemistry, 24, no. 1 (2019):61-70,
https://doi.org/10.1007/s00775-018-1628-7 . .

TY  - JOUR
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope
AU  - Jones, Glenville
AU  - Kaufmann, Martin
AU  - Kaluđerović, Jovana
AU  - Aleksić-Veličković, Vesna
AU  - Mandić, Ljuba M.
AU  - Glibetić, Marija
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/781
AB  - Objective: The objective of the present study was to determine if vitamin D intake and status are associated with pre-eclampsia in a country without a vitamin D fortification policy. Design: A case-control study of pregnancies with (case) and without (control) pre-eclampsia was conducted from January to April when UVB is minimal. Maternal and cord blood obtained at delivery were measured for plasma 25-hydroxycholecalciferol (25-OH-D-3), 3-epimer of 25-OH-D-3 (3-epi-25-OH-D-3) and 24,25-dihydroxycholecalciferol (24,25-(OH)(2)D-3) by LC-MS/MS and maternal 1,25-dihydroxyvitamin D (1,25-(OH) 2D). Differences between groups were tested with ANOVA and Bonferroni post hoc tests (P  lt  0.05). Setting: Clinical Center of Serbia. Subjects: Pregnant women with and without pre-eclampsia (n 60) and their infants. Results: Exogenous vitamin D intake (0.95-16.25 mu g/d (38-650 IU/d)) was not significantly different between groups. Women with pre-eclampsia delivered infants at an earlier gestational age and had significantly lower mean total plasma 25-hydroxyvitamin D (25-OH-D; case: 11.2 (SD 5.1); control: 16.1 (SD 5.7) ng/ml; P=0.0006), 25-OH-D-3 (case: 10.0 (SD 4.9); control: 14.2 (SD 5.8) ng/ml; P=0.002), 3-epi-25-OH-D-3 (case: 0.5 (SD 0.2); control: 0.7 (SD 0.2) ng/ml; P=0.0007) and 1,25-(OH)(2)D (case: 56.5 (SD 26.6); control: 81.0 (SD 25.7) pg/ml; P=0.018), while 24,25-(OH)(2)D-3 was not different between groups. Infants did not differ in total plasma 25-OH-D, 25-OH-D-3, 3-epi-25-OH-D-3 and 24,25-(OH)(2)D-3, but the mean proportion of 3-epi-25-OH-D-3 was higher in the infant case group (case: 7.9 (SD 1.1); control: 7.0 (SD 1.4) % of total 25-OH-D-3; P=0.005). Conclusions: A high prevalence of vitamin D deficiency, as defined by plasma 25-OH-D lt 12 ng/ml, was observed in 47 % of all mothers and 77 % of all infants. These data underscore the need for prenatal vitamin D supplementation and a food fortification policy in Serbia.
PB  - Cambridge Univ Press, Cambridge
T2  - Public Health Nutrition
T1  - Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy
EP  - 1835
IS  - 10
SP  - 1825
VL  - 20
DO  - 10.1017/S1368980016000409
ER  - 

@article{
author = "Đekić-Ivanković, Marija and Weiler, Hope and Jones, Glenville and Kaufmann, Martin and Kaluđerović, Jovana and Aleksić-Veličković, Vesna and Mandić, Ljuba M. and Glibetić, Marija",
year = "2017",
abstract = "Objective: The objective of the present study was to determine if vitamin D intake and status are associated with pre-eclampsia in a country without a vitamin D fortification policy. Design: A case-control study of pregnancies with (case) and without (control) pre-eclampsia was conducted from January to April when UVB is minimal. Maternal and cord blood obtained at delivery were measured for plasma 25-hydroxycholecalciferol (25-OH-D-3), 3-epimer of 25-OH-D-3 (3-epi-25-OH-D-3) and 24,25-dihydroxycholecalciferol (24,25-(OH)(2)D-3) by LC-MS/MS and maternal 1,25-dihydroxyvitamin D (1,25-(OH) 2D). Differences between groups were tested with ANOVA and Bonferroni post hoc tests (P  lt  0.05). Setting: Clinical Center of Serbia. Subjects: Pregnant women with and without pre-eclampsia (n 60) and their infants. Results: Exogenous vitamin D intake (0.95-16.25 mu g/d (38-650 IU/d)) was not significantly different between groups. Women with pre-eclampsia delivered infants at an earlier gestational age and had significantly lower mean total plasma 25-hydroxyvitamin D (25-OH-D; case: 11.2 (SD 5.1); control: 16.1 (SD 5.7) ng/ml; P=0.0006), 25-OH-D-3 (case: 10.0 (SD 4.9); control: 14.2 (SD 5.8) ng/ml; P=0.002), 3-epi-25-OH-D-3 (case: 0.5 (SD 0.2); control: 0.7 (SD 0.2) ng/ml; P=0.0007) and 1,25-(OH)(2)D (case: 56.5 (SD 26.6); control: 81.0 (SD 25.7) pg/ml; P=0.018), while 24,25-(OH)(2)D-3 was not different between groups. Infants did not differ in total plasma 25-OH-D, 25-OH-D-3, 3-epi-25-OH-D-3 and 24,25-(OH)(2)D-3, but the mean proportion of 3-epi-25-OH-D-3 was higher in the infant case group (case: 7.9 (SD 1.1); control: 7.0 (SD 1.4) % of total 25-OH-D-3; P=0.005). Conclusions: A high prevalence of vitamin D deficiency, as defined by plasma 25-OH-D lt 12 ng/ml, was observed in 47 % of all mothers and 77 % of all infants. These data underscore the need for prenatal vitamin D supplementation and a food fortification policy in Serbia.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Public Health Nutrition",
title = "Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy",
pages = "1835-1825",
number = "10",
volume = "20",
doi = "10.1017/S1368980016000409"
}

Đekić-Ivanković, M., Weiler, H., Jones, G., Kaufmann, M., Kaluđerović, J., Aleksić-Veličković, V., Mandić, L. M.,& Glibetić, M.. (2017). Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy. in Public Health Nutrition
Cambridge Univ Press, Cambridge., 20(10), 1825-1835.
https://doi.org/10.1017/S1368980016000409

Đekić-Ivanković M, Weiler H, Jones G, Kaufmann M, Kaluđerović J, Aleksić-Veličković V, Mandić LM, Glibetić M. Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy. in Public Health Nutrition. 2017;20(10):1825-1835.
doi:10.1017/S1368980016000409 .

Đekić-Ivanković, Marija, Weiler, Hope, Jones, Glenville, Kaufmann, Martin, Kaluđerović, Jovana, Aleksić-Veličković, Vesna, Mandić, Ljuba M., Glibetić, Marija, "Vitamin D status in mothers with pre-eclampsia and their infants: a case-control study from Serbia, a country without a vitamin D fortification policy" in Public Health Nutrition, 20, no. 10 (2017):1825-1835,
https://doi.org/10.1017/S1368980016000409 . .

TY  - JOUR
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Takić, Marija
AU  - Aćimović, Jelena M.
AU  - Penezić, Ana Z.
AU  - Mandić, Ljuba M.
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/698
AB  - Non-esterified fatty acids bound to the human serum albumin (HSA) contribute to several HSAs properties of special concern in pathologies, for instance to the reactivity of the free HSA-Cys34 thiol group (important antioxidative thiol pool in plasma), and to the affinity for binding of molecules and ions (for example cobalt as a prominent biomarker in heart ischemia). Therefore, the method for determination of FAs bound to HSA was developed. FAs were released from HSA (previously isolated from serum by ammonium sulfate precipitation) using acidic copper(II) sulfate in phosphoric acid, extracted by n-heptane-chloroform (4:1, v/v) mixture, spotted on TL silica-gel and then developed with n-heptane-chloroform-acetic acid (5:3:03, v/v/v). Common office flatbed scanner and software solution for densitometric image analysis, developed in R, were used. The linearity of calibration curve in concentration range from 0.1 to 5.0 mmol/L stearic acid was achieved. The method was proved to be precise (with RSD of 1.4-4.7%) and accurate. Accuracy was examined by standard addition method (recoveries 97.2-102.5%) and by comparison to results of GC. The method is sample saving, technically less demanding, and cheap, and therefore suitable for determination of FAs/HSA ratio when elevated concentrations of free FAs are reliable diagnostic/risk parameter of pathological states.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Pharmaceutical & Biomedical Analysis
T1  - Quantification of total content of non-esterified fatty acids bound to human serum albumin
EP  - 49
SP  - 43
VL  - 129
DO  - 10.1016/j.jpba.2016.06.043
ER  - 

@article{
author = "Pavićević, Ivan D. and Jovanović, Vesna B. and Takić, Marija and Aćimović, Jelena M. and Penezić, Ana Z. and Mandić, Ljuba M.",
year = "2016",
abstract = "Non-esterified fatty acids bound to the human serum albumin (HSA) contribute to several HSAs properties of special concern in pathologies, for instance to the reactivity of the free HSA-Cys34 thiol group (important antioxidative thiol pool in plasma), and to the affinity for binding of molecules and ions (for example cobalt as a prominent biomarker in heart ischemia). Therefore, the method for determination of FAs bound to HSA was developed. FAs were released from HSA (previously isolated from serum by ammonium sulfate precipitation) using acidic copper(II) sulfate in phosphoric acid, extracted by n-heptane-chloroform (4:1, v/v) mixture, spotted on TL silica-gel and then developed with n-heptane-chloroform-acetic acid (5:3:03, v/v/v). Common office flatbed scanner and software solution for densitometric image analysis, developed in R, were used. The linearity of calibration curve in concentration range from 0.1 to 5.0 mmol/L stearic acid was achieved. The method was proved to be precise (with RSD of 1.4-4.7%) and accurate. Accuracy was examined by standard addition method (recoveries 97.2-102.5%) and by comparison to results of GC. The method is sample saving, technically less demanding, and cheap, and therefore suitable for determination of FAs/HSA ratio when elevated concentrations of free FAs are reliable diagnostic/risk parameter of pathological states.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Pharmaceutical & Biomedical Analysis",
title = "Quantification of total content of non-esterified fatty acids bound to human serum albumin",
pages = "49-43",
volume = "129",
doi = "10.1016/j.jpba.2016.06.043"
}

Pavićević, I. D., Jovanović, V. B., Takić, M., Aćimović, J. M., Penezić, A. Z.,& Mandić, L. M.. (2016). Quantification of total content of non-esterified fatty acids bound to human serum albumin. in Journal of Pharmaceutical & Biomedical Analysis
Elsevier Science Bv, Amsterdam., 129, 43-49.
https://doi.org/10.1016/j.jpba.2016.06.043

Pavićević ID, Jovanović VB, Takić M, Aćimović JM, Penezić AZ, Mandić LM. Quantification of total content of non-esterified fatty acids bound to human serum albumin. in Journal of Pharmaceutical & Biomedical Analysis. 2016;129:43-49.
doi:10.1016/j.jpba.2016.06.043 .

Pavićević, Ivan D., Jovanović, Vesna B., Takić, Marija, Aćimović, Jelena M., Penezić, Ana Z., Mandić, Ljuba M., "Quantification of total content of non-esterified fatty acids bound to human serum albumin" in Journal of Pharmaceutical & Biomedical Analysis, 129 (2016):43-49,
https://doi.org/10.1016/j.jpba.2016.06.043 . .

TY  - JOUR
AU  - Takić, Marija
AU  - Jovanović, Vesna B.
AU  - Pavićević, Ivan D.
AU  - Uzelac, Tamara N.
AU  - Aćimović, Jelena M.
AU  - Ristić-Medić, Danijela
AU  - Mandić, Ljuba M.
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/730
AB  - The interaction of polyphenolic molecules with human serum albumin (HSA) could lead to changes in the reactivity of the HSA Cys34 thiol group (HSA-SH). The influences of enterolactone (EL) and enterodiol (ED) binding on HSA-SH reactivity in fatty acid (FA)-free HSA, and in HSA with bound stearic acid (S) in S/HSA molar ratios of 1 : 1 and 4 : 1, were investigated by the determination of the pseudo first order rate constants (k') for the thiol reaction with 5,5'-dithiobis-(2-nitrobenzoic acid). The binding affinities and binding sites of EL and ED were also determined, using fluorescence measurements of the intrinsic fluorescence of Trp214 and diazepam (binding site marker). EL and ED binding to HSA increased the reactivity of HSA-SH in all assayed HSA-enterolignan complexes by 9.1-33.1%. The strongest effects were obtained for FA-free HSA-enterolignan complexes. S modulated/reduced the effect of EL on HSA-SH reactivity, while its influence on the effect of ED was negligible. The binding of enterolignans to HSA was investigated: the binding constants were the highest for FA-free HSA (EL: 11.64 x 10(4) M-1 and ED: 5.59 x 10(4) M-1 at 37 degrees C) and the lowest for S/HSA 4 : 1-enterolignan complexes (EL: 2.43 x 10(4) M-1 and ED: 1.92 x 10(4) M-1). When the S/HSA ratio was increased, the binding affinities and number of binding sites for EL and ED were decreased. At the same time, a high correlation between binding constants and increased Cys34 reactivity was found (r = 0.974). Competitive experiments using diazepam indicated that the binding of ED and of EL was located in the hydrophobic pocket of site II in HSA. Overall, it is evident that stearic acid could modulate the enterolignan effects on HSA-SH reactivity as well as their binding to HSA. This finding could be important for pharmacokinetics and the expression of enterolignan antioxidant effects in vivo after an intake of lignan rich food.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity
EP  - 1226
IS  - 2
SP  - 1217
VL  - 7
DO  - 10.1039/c5fo01346a
ER  - 

@article{
author = "Takić, Marija and Jovanović, Vesna B. and Pavićević, Ivan D. and Uzelac, Tamara N. and Aćimović, Jelena M. and Ristić-Medić, Danijela and Mandić, Ljuba M.",
year = "2016",
abstract = "The interaction of polyphenolic molecules with human serum albumin (HSA) could lead to changes in the reactivity of the HSA Cys34 thiol group (HSA-SH). The influences of enterolactone (EL) and enterodiol (ED) binding on HSA-SH reactivity in fatty acid (FA)-free HSA, and in HSA with bound stearic acid (S) in S/HSA molar ratios of 1 : 1 and 4 : 1, were investigated by the determination of the pseudo first order rate constants (k') for the thiol reaction with 5,5'-dithiobis-(2-nitrobenzoic acid). The binding affinities and binding sites of EL and ED were also determined, using fluorescence measurements of the intrinsic fluorescence of Trp214 and diazepam (binding site marker). EL and ED binding to HSA increased the reactivity of HSA-SH in all assayed HSA-enterolignan complexes by 9.1-33.1%. The strongest effects were obtained for FA-free HSA-enterolignan complexes. S modulated/reduced the effect of EL on HSA-SH reactivity, while its influence on the effect of ED was negligible. The binding of enterolignans to HSA was investigated: the binding constants were the highest for FA-free HSA (EL: 11.64 x 10(4) M-1 and ED: 5.59 x 10(4) M-1 at 37 degrees C) and the lowest for S/HSA 4 : 1-enterolignan complexes (EL: 2.43 x 10(4) M-1 and ED: 1.92 x 10(4) M-1). When the S/HSA ratio was increased, the binding affinities and number of binding sites for EL and ED were decreased. At the same time, a high correlation between binding constants and increased Cys34 reactivity was found (r = 0.974). Competitive experiments using diazepam indicated that the binding of ED and of EL was located in the hydrophobic pocket of site II in HSA. Overall, it is evident that stearic acid could modulate the enterolignan effects on HSA-SH reactivity as well as their binding to HSA. This finding could be important for pharmacokinetics and the expression of enterolignan antioxidant effects in vivo after an intake of lignan rich food.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity",
pages = "1226-1217",
number = "2",
volume = "7",
doi = "10.1039/c5fo01346a"
}

Takić, M., Jovanović, V. B., Pavićević, I. D., Uzelac, T. N., Aćimović, J. M., Ristić-Medić, D.,& Mandić, L. M.. (2016). Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity. in Food & Function
Royal Soc Chemistry, Cambridge., 7(2), 1217-1226.
https://doi.org/10.1039/c5fo01346a

Takić M, Jovanović VB, Pavićević ID, Uzelac TN, Aćimović JM, Ristić-Medić D, Mandić LM. Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity. in Food & Function. 2016;7(2):1217-1226.
doi:10.1039/c5fo01346a .

Takić, Marija, Jovanović, Vesna B., Pavićević, Ivan D., Uzelac, Tamara N., Aćimović, Jelena M., Ristić-Medić, Danijela, Mandić, Ljuba M., "Binding of enterolactone and enterodiol to human serum albumin: increase of cysteine-34 thiol group reactivity" in Food & Function, 7, no. 2 (2016):1217-1226,
https://doi.org/10.1039/c5fo01346a . .

TY  - CONF
AU  - Aćimović, Jelena M.
AU  - Penezić, Ana Z.
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Takić, Marija
AU  - Uzelac, Tamara N.
AU  - Mandić, Ljuba M.
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/739
PB  - Wiley-Blackwell, Hoboken
C3  - FEBS Journal
T1  - Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal
EP  - 417
SP  - 417
VL  - 283
UR  - https://hdl.handle.net/21.15107/rcub_rimi_739
ER  - 

@conference{
author = "Aćimović, Jelena M. and Penezić, Ana Z. and Pavićević, Ivan D. and Jovanović, Vesna B. and Takić, Marija and Uzelac, Tamara N. and Mandić, Ljuba M.",
year = "2016",
publisher = "Wiley-Blackwell, Hoboken",
journal = "FEBS Journal",
title = "Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal",
pages = "417-417",
volume = "283",
url = "https://hdl.handle.net/21.15107/rcub_rimi_739"
}

Aćimović, J. M., Penezić, A. Z., Pavićević, I. D., Jovanović, V. B., Takić, M., Uzelac, T. N.,& Mandić, L. M.. (2016). Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal. in FEBS Journal
Wiley-Blackwell, Hoboken., 283, 417-417.
https://hdl.handle.net/21.15107/rcub_rimi_739

Aćimović JM, Penezić AZ, Pavićević ID, Jovanović VB, Takić M, Uzelac TN, Mandić LM. Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal. in FEBS Journal. 2016;283:417-417.
https://hdl.handle.net/21.15107/rcub_rimi_739 .

Aćimović, Jelena M., Penezić, Ana Z., Pavićević, Ivan D., Jovanović, Vesna B., Takić, Marija, Uzelac, Tamara N., Mandić, Ljuba M., "Binding of FAs and Cu(II) ions to HSA changes its Cys34 thiol group antioxidant capacity and carbonylation pattern with methylglyoxal" in FEBS Journal, 283 (2016):417-417,
https://hdl.handle.net/21.15107/rcub_rimi_739 .

TY  - JOUR
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope
AU  - Nikolić, Marina
AU  - Kadvan, Agnes
AU  - Gurinović, Mirjana
AU  - Mandić, Ljuba M.
AU  - Glibetić, Marija
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/729
AB  - Objective: The objective of the present study was to examine the external validity of an FFQ designed to estimate dietary vitamin D intake compared with a plasma biomarker and three repeated 24 h dietary recalls in women of reproductive age in Serbia, where there is no exposure to food fortified with vitamin D. The method of triads was applied. Design: In a cross-sectional study, 422 women completed the Women and Reproductive Health FFQ (WRH-FFQ) during the winter months. From a representative subgroup (n 44), three 24 h dietary recalls and anthropometric parameters were collected as well as a fasting blood sample for vitamin D biomarker analyses. Correlation coefficients were calculated between each of the dietary methods. Validity coefficients, as a correlation between the measured and estimated 'true' exposure, were calculated using the method of triads. Bland-Altman plots were also constructed. Setting: Three major universities in Serbia. Subjects: Healthy young women (n 422) aged 18-35 years. Results: The WRH-FFQ estimate of vitamin D intake for all participants was 4.0 (SD 3.3) mu g/d and 3.1 (SD 2.3) mu g/d for the subgroup. Bland-Altman plots for these intakes showed high agreement. Validity coefficients for the FFQ, 24 h recall and biomarker were. rho(QI) = 0.847 (95 % CI 0.564, 0.928), rho(RI) = 0.810 (95 % CI 0.537, 0.997) and rho(BI) = 0.499 (95 % CI 0.190, 0.840), while the correlation coefficients were 0.686, 0.422 and 0.404. Conclusions: The FFQ applied in the present study is a valid tool for assessing dietary vitamin D intake in women living in Serbia, a region without mandatory vitamin D food fortification.
PB  - Cambridge Univ Press, Cambridge
T2  - Public Health Nutrition
T1  - Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model
EP  - 445
IS  - 4
SP  - 437
VL  - 19
DO  - 10.1017/S136898001500138X
ER  - 

@article{
author = "Đekić-Ivanković, Marija and Weiler, Hope and Nikolić, Marina and Kadvan, Agnes and Gurinović, Mirjana and Mandić, Ljuba M. and Glibetić, Marija",
year = "2016",
abstract = "Objective: The objective of the present study was to examine the external validity of an FFQ designed to estimate dietary vitamin D intake compared with a plasma biomarker and three repeated 24 h dietary recalls in women of reproductive age in Serbia, where there is no exposure to food fortified with vitamin D. The method of triads was applied. Design: In a cross-sectional study, 422 women completed the Women and Reproductive Health FFQ (WRH-FFQ) during the winter months. From a representative subgroup (n 44), three 24 h dietary recalls and anthropometric parameters were collected as well as a fasting blood sample for vitamin D biomarker analyses. Correlation coefficients were calculated between each of the dietary methods. Validity coefficients, as a correlation between the measured and estimated 'true' exposure, were calculated using the method of triads. Bland-Altman plots were also constructed. Setting: Three major universities in Serbia. Subjects: Healthy young women (n 422) aged 18-35 years. Results: The WRH-FFQ estimate of vitamin D intake for all participants was 4.0 (SD 3.3) mu g/d and 3.1 (SD 2.3) mu g/d for the subgroup. Bland-Altman plots for these intakes showed high agreement. Validity coefficients for the FFQ, 24 h recall and biomarker were. rho(QI) = 0.847 (95 % CI 0.564, 0.928), rho(RI) = 0.810 (95 % CI 0.537, 0.997) and rho(BI) = 0.499 (95 % CI 0.190, 0.840), while the correlation coefficients were 0.686, 0.422 and 0.404. Conclusions: The FFQ applied in the present study is a valid tool for assessing dietary vitamin D intake in women living in Serbia, a region without mandatory vitamin D food fortification.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Public Health Nutrition",
title = "Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model",
pages = "445-437",
number = "4",
volume = "19",
doi = "10.1017/S136898001500138X"
}

Đekić-Ivanković, M., Weiler, H., Nikolić, M., Kadvan, A., Gurinović, M., Mandić, L. M.,& Glibetić, M.. (2016). Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model. in Public Health Nutrition
Cambridge Univ Press, Cambridge., 19(4), 437-445.
https://doi.org/10.1017/S136898001500138X

Đekić-Ivanković M, Weiler H, Nikolić M, Kadvan A, Gurinović M, Mandić LM, Glibetić M. Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model. in Public Health Nutrition. 2016;19(4):437-445.
doi:10.1017/S136898001500138X .

Đekić-Ivanković, Marija, Weiler, Hope, Nikolić, Marina, Kadvan, Agnes, Gurinović, Mirjana, Mandić, Ljuba M., Glibetić, Marija, "Validity of an FFQ assessing the vitamin D intake of young Serbian women living in a region without food fortification: the method of triads model" in Public Health Nutrition, 19, no. 4 (2016):437-445,
https://doi.org/10.1017/S136898001500138X . .

TY  - CONF
AU  - Đekić-Ivanković, Marija
AU  - Weiler, Hope
AU  - Jones, Glenville
AU  - Kaufmann, Martin
AU  - Kaluđerović, Jovana
AU  - Aleksić-Veličković, Vesna
AU  - Mandić, Ljuba M.
AU  - Glibetić, Marija
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/628
PB  - Federation Amer Soc Exp Biol, Bethesda
C3  - FASEB Journal
T1  - Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia
VL  - 29
UR  - https://hdl.handle.net/21.15107/rcub_rimi_628
ER  - 

@conference{
author = "Đekić-Ivanković, Marija and Weiler, Hope and Jones, Glenville and Kaufmann, Martin and Kaluđerović, Jovana and Aleksić-Veličković, Vesna and Mandić, Ljuba M. and Glibetić, Marija",
year = "2015",
publisher = "Federation Amer Soc Exp Biol, Bethesda",
journal = "FASEB Journal",
title = "Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia",
volume = "29",
url = "https://hdl.handle.net/21.15107/rcub_rimi_628"
}

Đekić-Ivanković, M., Weiler, H., Jones, G., Kaufmann, M., Kaluđerović, J., Aleksić-Veličković, V., Mandić, L. M.,& Glibetić, M.. (2015). Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia. in FASEB Journal
Federation Amer Soc Exp Biol, Bethesda., 29.
https://hdl.handle.net/21.15107/rcub_rimi_628

Đekić-Ivanković M, Weiler H, Jones G, Kaufmann M, Kaluđerović J, Aleksić-Veličković V, Mandić LM, Glibetić M. Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia. in FASEB Journal. 2015;29.
https://hdl.handle.net/21.15107/rcub_rimi_628 .

Đekić-Ivanković, Marija, Weiler, Hope, Jones, Glenville, Kaufmann, Martin, Kaluđerović, Jovana, Aleksić-Veličković, Vesna, Mandić, Ljuba M., Glibetić, Marija, "Vitamin D Status is Low in Mothers with Preeclampsia and Their Infants: a Case Control Study from Serbia" in FASEB Journal, 29 (2015),
https://hdl.handle.net/21.15107/rcub_rimi_628 .

TY  - JOUR
AU  - Pavićević, Ivan D.
AU  - Jovanović, Vesna B.
AU  - Takić, Marija
AU  - Penezić, Ana Z.
AU  - Aćimović, Jelena M.
AU  - Mandić, Ljuba M.
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/534
AB  - Fatty acids (FAs) binding to human serum albumin (HSA) could lead to the changes of Cys-34 thiol group accessibility and reactivity, i.e. its scavenger capacity and antioxidant property. The influence of saturated, mono and poly unsaturated, and fish oil FAs binding to HSA on the carbonylation level and the reactivity of HSA-SH and HSA modified with methylglyoxal (MG-HSA-SH) was investigated. Changes of thiol group reactivity were followed by determination of pseudo first order rate constant (k') for thiols reaction with 5,5'-dithiobis(2-nitrobenzoic acid). HSA changes were monitored using native PAG electrophoresis and fluorescence spectroscopy. For FA/HSA molar ratios screening, qTLC and GC were used. FAs increase thiol group carbonylation levels from 8% to 20%. The k' values obtained for FAs-free HSA-SH and FAs-free MG-HSA-SH are almost equal (7.5 x 10(-3) and 7.7 x 10(-3) resp.). Binding of all FAs amplify the reactivity (k' values from 14.6 x 10(-3) to 26.0 x 10(-3) s(-1)) of HSA-SH group for 2-3.5 times in the order: palmitic, docosahexaenoic, fish oil extract, stearic, oleic, myristic and eicosapentaenoic acid, due to HSA conformational changes. FAs-bound MG-HSA-SH samples follow that pattern, but their k' values (from 9.8 x 10(-3) to 14.3 x 10(-3) s(-1)) were lower compared to unmodified HSA due to additional conformation changes of HSA molecules during carbonylation. Carbonylation level and reactivity of Cys34 thiol group of unmodified and carbonylated HSA depend on type of FAs bound to HSA, which implies the possibility for modulation of -SH reactivity (scavenger capacity and antioxidant property) by FAs as a supplement.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal
EP  - 50
SP  - 42
VL  - 224
DO  - 10.1016/j.cbi.2014.10.008
ER  - 

@article{
author = "Pavićević, Ivan D. and Jovanović, Vesna B. and Takić, Marija and Penezić, Ana Z. and Aćimović, Jelena M. and Mandić, Ljuba M.",
year = "2014",
abstract = "Fatty acids (FAs) binding to human serum albumin (HSA) could lead to the changes of Cys-34 thiol group accessibility and reactivity, i.e. its scavenger capacity and antioxidant property. The influence of saturated, mono and poly unsaturated, and fish oil FAs binding to HSA on the carbonylation level and the reactivity of HSA-SH and HSA modified with methylglyoxal (MG-HSA-SH) was investigated. Changes of thiol group reactivity were followed by determination of pseudo first order rate constant (k') for thiols reaction with 5,5'-dithiobis(2-nitrobenzoic acid). HSA changes were monitored using native PAG electrophoresis and fluorescence spectroscopy. For FA/HSA molar ratios screening, qTLC and GC were used. FAs increase thiol group carbonylation levels from 8% to 20%. The k' values obtained for FAs-free HSA-SH and FAs-free MG-HSA-SH are almost equal (7.5 x 10(-3) and 7.7 x 10(-3) resp.). Binding of all FAs amplify the reactivity (k' values from 14.6 x 10(-3) to 26.0 x 10(-3) s(-1)) of HSA-SH group for 2-3.5 times in the order: palmitic, docosahexaenoic, fish oil extract, stearic, oleic, myristic and eicosapentaenoic acid, due to HSA conformational changes. FAs-bound MG-HSA-SH samples follow that pattern, but their k' values (from 9.8 x 10(-3) to 14.3 x 10(-3) s(-1)) were lower compared to unmodified HSA due to additional conformation changes of HSA molecules during carbonylation. Carbonylation level and reactivity of Cys34 thiol group of unmodified and carbonylated HSA depend on type of FAs bound to HSA, which implies the possibility for modulation of -SH reactivity (scavenger capacity and antioxidant property) by FAs as a supplement.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal",
pages = "50-42",
volume = "224",
doi = "10.1016/j.cbi.2014.10.008"
}

Pavićević, I. D., Jovanović, V. B., Takić, M., Penezić, A. Z., Aćimović, J. M.,& Mandić, L. M.. (2014). Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 224, 42-50.
https://doi.org/10.1016/j.cbi.2014.10.008

Pavićević ID, Jovanović VB, Takić M, Penezić AZ, Aćimović JM, Mandić LM. Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal. in Chemico-Biological Interactions. 2014;224:42-50.
doi:10.1016/j.cbi.2014.10.008 .

Pavićević, Ivan D., Jovanović, Vesna B., Takić, Marija, Penezić, Ana Z., Aćimović, Jelena M., Mandić, Ljuba M., "Fatty acids binding to human serum albumin: Changes of reactivity and glycation level of Cysteine-34 free thiol group with methylglyoxal" in Chemico-Biological Interactions, 224 (2014):42-50,
https://doi.org/10.1016/j.cbi.2014.10.008 . .

TY  - JOUR
AU  - Jovanović, Vesna B.
AU  - Pavićević, Ivan D.
AU  - Takić, Marija
AU  - Penezic-Romanjuk, Ana Z.
AU  - Aćimović, Jelena M.
AU  - Mandić, Ljuba M.
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/558
AB  - During investigation of the changes of the Cys34 thiol group of human serum albumin (HSA) (isolated by affinity chromatography with Cibacron Blue (CB)) in diabetes, we found that the HSA-SH content was higher (11-33%) than the total serum thiol content. The influence of fatty acids (FA) binding to HSA on this discrepancy was investigated in vitro (using fluorescence and CD spectroscopy and GC) and with HSA samples from diabetic (n=20) and control groups (n=17). HSA-bound FA determine the selection of HSA molecules by CB and enhance reactivity and/or accessibility of the SH group. A high content of polyunsaturated FA (35.6%) leads to weaker binding of HSA molecules to CB. Rate constants of DTNB reaction with the SH group of HSA applied to a CB column, bound-HSA and unbound-HSA fractions, were 4.8 x 10(-3), 21.6 x 10(-3), and 11.2 x 10(-3) s(-1), respectively. The HSA-SH group of diabetics is more reactive compared with control individuals (rate constants 20.9 x 10(-3)+/- 4.4 x 10(-3) vs 12.9 x 10(-3)+/- 2.6 x 10(-3) s(-1), P lt 0.05). Recovery values of the SH group obtained after chromatography of HSA with bound stearic acid ranged from 110 to 140%, while those for defatted HSA were from 98.5 to 101.7%. Thus, HSA-bound FA leads to an increase of HSA-SH content and a contribution to total serum thiols, which make the determination of the thiol group unreliable.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - The influence of fatty acids on determination of human serum albumin thiol group
EP  - 57
SP  - 50
VL  - 448
DO  - 10.1016/j.ab.2013.11.030
ER  - 

@article{
author = "Jovanović, Vesna B. and Pavićević, Ivan D. and Takić, Marija and Penezic-Romanjuk, Ana Z. and Aćimović, Jelena M. and Mandić, Ljuba M.",
year = "2014",
abstract = "During investigation of the changes of the Cys34 thiol group of human serum albumin (HSA) (isolated by affinity chromatography with Cibacron Blue (CB)) in diabetes, we found that the HSA-SH content was higher (11-33%) than the total serum thiol content. The influence of fatty acids (FA) binding to HSA on this discrepancy was investigated in vitro (using fluorescence and CD spectroscopy and GC) and with HSA samples from diabetic (n=20) and control groups (n=17). HSA-bound FA determine the selection of HSA molecules by CB and enhance reactivity and/or accessibility of the SH group. A high content of polyunsaturated FA (35.6%) leads to weaker binding of HSA molecules to CB. Rate constants of DTNB reaction with the SH group of HSA applied to a CB column, bound-HSA and unbound-HSA fractions, were 4.8 x 10(-3), 21.6 x 10(-3), and 11.2 x 10(-3) s(-1), respectively. The HSA-SH group of diabetics is more reactive compared with control individuals (rate constants 20.9 x 10(-3)+/- 4.4 x 10(-3) vs 12.9 x 10(-3)+/- 2.6 x 10(-3) s(-1), P lt 0.05). Recovery values of the SH group obtained after chromatography of HSA with bound stearic acid ranged from 110 to 140%, while those for defatted HSA were from 98.5 to 101.7%. Thus, HSA-bound FA leads to an increase of HSA-SH content and a contribution to total serum thiols, which make the determination of the thiol group unreliable.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "The influence of fatty acids on determination of human serum albumin thiol group",
pages = "57-50",
volume = "448",
doi = "10.1016/j.ab.2013.11.030"
}

Jovanović, V. B., Pavićević, I. D., Takić, M., Penezic-Romanjuk, A. Z., Aćimović, J. M.,& Mandić, L. M.. (2014). The influence of fatty acids on determination of human serum albumin thiol group. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 448, 50-57.
https://doi.org/10.1016/j.ab.2013.11.030

Jovanović VB, Pavićević ID, Takić M, Penezic-Romanjuk AZ, Aćimović JM, Mandić LM. The influence of fatty acids on determination of human serum albumin thiol group. in Analytical Biochemistry. 2014;448:50-57.
doi:10.1016/j.ab.2013.11.030 .

Jovanović, Vesna B., Pavićević, Ivan D., Takić, Marija, Penezic-Romanjuk, Ana Z., Aćimović, Jelena M., Mandić, Ljuba M., "The influence of fatty acids on determination of human serum albumin thiol group" in Analytical Biochemistry, 448 (2014):50-57,
https://doi.org/10.1016/j.ab.2013.11.030 . .

TY  - JOUR
AU  - Popović, Tamara B.
AU  - Borozan, Sunčica
AU  - Arsić, Aleksandra
AU  - Martacić, J. D.
AU  - Vučić, Vesna M.
AU  - Trbović, A.
AU  - Mandić, Ljuba M.
AU  - Glibetić, Marija
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/405
AB  - In the present study, we examined the effects of fish oil supplementation in 3 months old male Wistar rats on changes in plasma and liver lipid metabolism and oxidative stress parameters. Twenty Wistar rats were randomly divided into two groups of ten animals: control group and intervention group, treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid and 30 mg docosahexanoic acid. After intervention, biochemical parameters in plasma [triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL) and total cholesterol, urea, creatinine and uric acid], fatty acid (FAs) profile of liver phospholipids and parameters of oxidative stress in liver [activity of catalase, superoxide dismutase and paraoxonase (PON1), concentration of nitrites, lipid peroxidation (LPO), free thiol (SH) groups and lactate dehydrogenase (LDH) izoenzymes were determined. Treatment with fish oil improved FAs profile of liver phospholipids, increasing n-3 FAs and decreasing n-6/n-3 ratio. Significant decrease in plasma TG and LDL concentration, and increase in the level of HDL and uric acid were found in intervention group at the end of the study. Catalase activity, LPO, and nitrites concentration in liver were significantly decreased, after the supplementation, together with elevated PON1 activity. Applied treatment significantly improved plasma lipid profile, liver FAs composition and parameters of oxidative stress in male Wistar rats.
PB  - Wiley, Hoboken
T2  - Journal of Animal Physiology & Animal Nutrition
T1  - Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats
EP  - 1029
IS  - 6
SP  - 1020
VL  - 96
DO  - 10.1111/j.1439-0396.2011.01216.x
ER  - 

@article{
author = "Popović, Tamara B. and Borozan, Sunčica and Arsić, Aleksandra and Martacić, J. D. and Vučić, Vesna M. and Trbović, A. and Mandić, Ljuba M. and Glibetić, Marija",
year = "2012",
abstract = "In the present study, we examined the effects of fish oil supplementation in 3 months old male Wistar rats on changes in plasma and liver lipid metabolism and oxidative stress parameters. Twenty Wistar rats were randomly divided into two groups of ten animals: control group and intervention group, treated for 6 weeks with fish oil capsules containing 45 mg eicosapentanoic acid and 30 mg docosahexanoic acid. After intervention, biochemical parameters in plasma [triglycerides (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL) and total cholesterol, urea, creatinine and uric acid], fatty acid (FAs) profile of liver phospholipids and parameters of oxidative stress in liver [activity of catalase, superoxide dismutase and paraoxonase (PON1), concentration of nitrites, lipid peroxidation (LPO), free thiol (SH) groups and lactate dehydrogenase (LDH) izoenzymes were determined. Treatment with fish oil improved FAs profile of liver phospholipids, increasing n-3 FAs and decreasing n-6/n-3 ratio. Significant decrease in plasma TG and LDL concentration, and increase in the level of HDL and uric acid were found in intervention group at the end of the study. Catalase activity, LPO, and nitrites concentration in liver were significantly decreased, after the supplementation, together with elevated PON1 activity. Applied treatment significantly improved plasma lipid profile, liver FAs composition and parameters of oxidative stress in male Wistar rats.",
publisher = "Wiley, Hoboken",
journal = "Journal of Animal Physiology & Animal Nutrition",
title = "Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats",
pages = "1029-1020",
number = "6",
volume = "96",
doi = "10.1111/j.1439-0396.2011.01216.x"
}

Popović, T. B., Borozan, S., Arsić, A., Martacić, J. D., Vučić, V. M., Trbović, A., Mandić, L. M.,& Glibetić, M.. (2012). Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats. in Journal of Animal Physiology & Animal Nutrition
Wiley, Hoboken., 96(6), 1020-1029.
https://doi.org/10.1111/j.1439-0396.2011.01216.x

Popović TB, Borozan S, Arsić A, Martacić JD, Vučić VM, Trbović A, Mandić LM, Glibetić M. Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats. in Journal of Animal Physiology & Animal Nutrition. 2012;96(6):1020-1029.
doi:10.1111/j.1439-0396.2011.01216.x .

Popović, Tamara B., Borozan, Sunčica, Arsić, Aleksandra, Martacić, J. D., Vučić, Vesna M., Trbović, A., Mandić, Ljuba M., Glibetić, Marija, "Fish oil supplementation improved liver phospholipids fatty acid composition and parameters of oxidative stress in male wistar rats" in Journal of Animal Physiology & Animal Nutrition, 96, no. 6 (2012):1020-1029,
https://doi.org/10.1111/j.1439-0396.2011.01216.x . .

TY  - JOUR
AU  - Takić, Marija
AU  - Ristić-Medić, Danijela
AU  - Mandić, Ljuba M.
AU  - Glibetić, Marija
AU  - Arsić, Aleksandra
AU  - Debeljak-Martačić, Jasmina
AU  - Petrović-Oggiano, Gordana P.
PY  - 2009
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/247
AB  - This study examines the relationship between erythrocyte phospholipid FA composition and insulin sensitiv­ity in obese patients on a typical Serbian diet. In 30 patients, two insulin sensitivity groups were formed and their FAs analyzed. The shares of 22:5n-3, 22:6n-3, and total n-3 PUFAs, as well as a measure of Δ5 desaturase activity (the 20:4n-6/20:3n-6 ratio) were lower in the insulin resistance group compared to the normal glucose tolerance group. The fasting insulin level and HOMA value were inversely related to the percentage of 22:5n-3, 22:6n-3, and total n-3 PUFAs. Our data indicate that the percentage of n-3 PUFAs in erythrocyte phospholipids is linked with insulin sensitivity parameters in obese patients.
AB  - Masnokiselinski sastav membrana, koji se bar delimično može modifikovati dijetarnim unosom masnih kiselina, igra važnu ulogu u senzitivnosti na insulin. Cilj ove studije bio je ispitivanje veze između senzitivnosti na insulin i masnokiselinskog sastava fosfolipida eritrocita kod gojaznih osoba. Trideset umereno gojaznih/gojaznih nedijabetičara sa hiperlipoproteinemijama (ukupni holesterol  gt  5.2 mmol/l, LDL-holesterol  gt  3.4 mmol/l) podeljeni su u dve grupe, koristeći HOMA vrednost kao parametar senzitivnosti na insulin: grupu sa normalnom tolerancom na glukozu (NTG) i grupu sa rezistencijom na insulin (IR). Ukupni lipidi eritrocita su estrahovani i fosfolipidi izolovani tankoslojnom hromatografijom. Posle metilovanja masne kiseline analizirane su gasno-tečnom hromatografijom. Zastupljenost 22:5n-3 (p  lt  0.01), 22:6n-3 (p  lt  0.01), ukupnih n-3 polinezasićenih masnih kiselina (p  lt  0.001) i odnos zastupljenosti 20:4n-6/20:3n-6 (pokazatelja aktivnosti delta-5 desaturaze) (p  lt  0.05) su sniženi u IR grupi u poređenju sa NGT grupom. Nivo insulina natašte i HOMA vrednost inverntno su povezani sa zastupljenošću 22:5n-3 (p  lt  0.05), 22:6n-3 (p  lt  0.01) i ukupnim n-3 polinezasićenim kiselinama (p  lt  0.01). Naši rezultati pokazuju da je nivo n-3 masnih kiselina u fosfolipidima eritrocita povezan sa senzitivnošću na insulin kod gojaznih osoba na ishrani tipičnoj za podneblje Srbije.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - N-3 polyunsaturated acids in erythrocyte phospholipids are associated with insulin sensitivity in obese patients on a typical Serbian diet
T1  - Zastupljenost n-3 polinezasićenih masnih kiselina u fosfolipidima eritrocita i insulinska senzitivnost kod gojaznih osoba pri tipičnoj ishrani podneblja Srbije
EP  - 43
IS  - 1
SP  - 37
VL  - 61
DO  - 10.2298/ABS0901037T
ER  - 

@article{
author = "Takić, Marija and Ristić-Medić, Danijela and Mandić, Ljuba M. and Glibetić, Marija and Arsić, Aleksandra and Debeljak-Martačić, Jasmina and Petrović-Oggiano, Gordana P.",
year = "2009",
abstract = "This study examines the relationship between erythrocyte phospholipid FA composition and insulin sensitiv­ity in obese patients on a typical Serbian diet. In 30 patients, two insulin sensitivity groups were formed and their FAs analyzed. The shares of 22:5n-3, 22:6n-3, and total n-3 PUFAs, as well as a measure of Δ5 desaturase activity (the 20:4n-6/20:3n-6 ratio) were lower in the insulin resistance group compared to the normal glucose tolerance group. The fasting insulin level and HOMA value were inversely related to the percentage of 22:5n-3, 22:6n-3, and total n-3 PUFAs. Our data indicate that the percentage of n-3 PUFAs in erythrocyte phospholipids is linked with insulin sensitivity parameters in obese patients., Masnokiselinski sastav membrana, koji se bar delimično može modifikovati dijetarnim unosom masnih kiselina, igra važnu ulogu u senzitivnosti na insulin. Cilj ove studije bio je ispitivanje veze između senzitivnosti na insulin i masnokiselinskog sastava fosfolipida eritrocita kod gojaznih osoba. Trideset umereno gojaznih/gojaznih nedijabetičara sa hiperlipoproteinemijama (ukupni holesterol  gt  5.2 mmol/l, LDL-holesterol  gt  3.4 mmol/l) podeljeni su u dve grupe, koristeći HOMA vrednost kao parametar senzitivnosti na insulin: grupu sa normalnom tolerancom na glukozu (NTG) i grupu sa rezistencijom na insulin (IR). Ukupni lipidi eritrocita su estrahovani i fosfolipidi izolovani tankoslojnom hromatografijom. Posle metilovanja masne kiseline analizirane su gasno-tečnom hromatografijom. Zastupljenost 22:5n-3 (p  lt  0.01), 22:6n-3 (p  lt  0.01), ukupnih n-3 polinezasićenih masnih kiselina (p  lt  0.001) i odnos zastupljenosti 20:4n-6/20:3n-6 (pokazatelja aktivnosti delta-5 desaturaze) (p  lt  0.05) su sniženi u IR grupi u poređenju sa NGT grupom. Nivo insulina natašte i HOMA vrednost inverntno su povezani sa zastupljenošću 22:5n-3 (p  lt  0.05), 22:6n-3 (p  lt  0.01) i ukupnim n-3 polinezasićenim kiselinama (p  lt  0.01). Naši rezultati pokazuju da je nivo n-3 masnih kiselina u fosfolipidima eritrocita povezan sa senzitivnošću na insulin kod gojaznih osoba na ishrani tipičnoj za podneblje Srbije.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "N-3 polyunsaturated acids in erythrocyte phospholipids are associated with insulin sensitivity in obese patients on a typical Serbian diet, Zastupljenost n-3 polinezasićenih masnih kiselina u fosfolipidima eritrocita i insulinska senzitivnost kod gojaznih osoba pri tipičnoj ishrani podneblja Srbije",
pages = "43-37",
number = "1",
volume = "61",
doi = "10.2298/ABS0901037T"
}

Takić, M., Ristić-Medić, D., Mandić, L. M., Glibetić, M., Arsić, A., Debeljak-Martačić, J.,& Petrović-Oggiano, G. P.. (2009). N-3 polyunsaturated acids in erythrocyte phospholipids are associated with insulin sensitivity in obese patients on a typical Serbian diet. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 61(1), 37-43.
https://doi.org/10.2298/ABS0901037T

Takić M, Ristić-Medić D, Mandić LM, Glibetić M, Arsić A, Debeljak-Martačić J, Petrović-Oggiano GP. N-3 polyunsaturated acids in erythrocyte phospholipids are associated with insulin sensitivity in obese patients on a typical Serbian diet. in Archives of Biological Sciences. 2009;61(1):37-43.
doi:10.2298/ABS0901037T .

Takić, Marija, Ristić-Medić, Danijela, Mandić, Ljuba M., Glibetić, Marija, Arsić, Aleksandra, Debeljak-Martačić, Jasmina, Petrović-Oggiano, Gordana P., "N-3 polyunsaturated acids in erythrocyte phospholipids are associated with insulin sensitivity in obese patients on a typical Serbian diet" in Archives of Biological Sciences, 61, no. 1 (2009):37-43,
https://doi.org/10.2298/ABS0901037T . .