Specific Binding of Paraprotein to Platelet Receptors as a Cause of Platelet Dysfunction in Monoclonal Gammopathies
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2013
Authors
Đunić, I.Elezović, Ivo
Vucić, M.
Srdić-Rajić, Tatjana
Konić-Ristić, Aleksandra
Ilić, Vesna
Milić, Nataša
Bila, Jelena
Suvajdžić-Vuković, Nada
Virijević, Marijana
Antić, Darko
Vidović, Ana
Tomin, Dragica
Article (Published version)
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Show full item recordAbstract
The study included 48 untreated patients with monoclonal gammopathies (MG). Paraprotein was isolated from the serum of 10 patients with decreased platelet aggregation. Platelet aggregation was measured before and after the addition of the isolated paraprotein to platelet-rich plasma (PRP) from 10 healthy donors, in vitro. Expression of platelet von Willebrand factor (vWF) receptor glycoprotein (GP)Ib and platelet collagen receptor GPVI was determined by flow cytometry in the PRP of healthy donors before and after the addition of isolated paraprotein using the monoclonal antibodies, CD42b (for GPIb) and CD36 (for GPVI). Flowcytometry showed that expression of CD42b and CD36 positive cells was reduced after the addition of isolated paraprotein to PRP from healthy donors (p lt 0.001). These investigations demonstrated that paraprotein causes platelet dysfunction in patients with MG due to specific binding to the platelet vWF receptor GPIb and platelet collagen receptor GPVI. Copyright (...c) 2013 S. Karger AG, Basel
Keywords:
Paraprotein-specific binding / Platelet dysfunction / Monoclonal gammopathiesSource:
Acta Haematologica, 2013, 130, 2, 101-107Publisher:
- Karger, Basel
DOI: 10.1159/000345418
ISSN: 0001-5792
PubMed: 23548666
WoS: 000323806000008
Scopus: 2-s2.0-84875637890
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Institut za medicinska istraživanjaTY - JOUR AU - Đunić, I. AU - Elezović, Ivo AU - Vucić, M. AU - Srdić-Rajić, Tatjana AU - Konić-Ristić, Aleksandra AU - Ilić, Vesna AU - Milić, Nataša AU - Bila, Jelena AU - Suvajdžić-Vuković, Nada AU - Virijević, Marijana AU - Antić, Darko AU - Vidović, Ana AU - Tomin, Dragica PY - 2013 UR - http://rimi.imi.bg.ac.rs/handle/123456789/510 AB - The study included 48 untreated patients with monoclonal gammopathies (MG). Paraprotein was isolated from the serum of 10 patients with decreased platelet aggregation. Platelet aggregation was measured before and after the addition of the isolated paraprotein to platelet-rich plasma (PRP) from 10 healthy donors, in vitro. Expression of platelet von Willebrand factor (vWF) receptor glycoprotein (GP)Ib and platelet collagen receptor GPVI was determined by flow cytometry in the PRP of healthy donors before and after the addition of isolated paraprotein using the monoclonal antibodies, CD42b (for GPIb) and CD36 (for GPVI). Flowcytometry showed that expression of CD42b and CD36 positive cells was reduced after the addition of isolated paraprotein to PRP from healthy donors (p lt 0.001). These investigations demonstrated that paraprotein causes platelet dysfunction in patients with MG due to specific binding to the platelet vWF receptor GPIb and platelet collagen receptor GPVI. Copyright (c) 2013 S. Karger AG, Basel PB - Karger, Basel T2 - Acta Haematologica T1 - Specific Binding of Paraprotein to Platelet Receptors as a Cause of Platelet Dysfunction in Monoclonal Gammopathies EP - 107 IS - 2 SP - 101 VL - 130 DO - 10.1159/000345418 ER -
@article{ author = "Đunić, I. and Elezović, Ivo and Vucić, M. and Srdić-Rajić, Tatjana and Konić-Ristić, Aleksandra and Ilić, Vesna and Milić, Nataša and Bila, Jelena and Suvajdžić-Vuković, Nada and Virijević, Marijana and Antić, Darko and Vidović, Ana and Tomin, Dragica", year = "2013", abstract = "The study included 48 untreated patients with monoclonal gammopathies (MG). Paraprotein was isolated from the serum of 10 patients with decreased platelet aggregation. Platelet aggregation was measured before and after the addition of the isolated paraprotein to platelet-rich plasma (PRP) from 10 healthy donors, in vitro. Expression of platelet von Willebrand factor (vWF) receptor glycoprotein (GP)Ib and platelet collagen receptor GPVI was determined by flow cytometry in the PRP of healthy donors before and after the addition of isolated paraprotein using the monoclonal antibodies, CD42b (for GPIb) and CD36 (for GPVI). Flowcytometry showed that expression of CD42b and CD36 positive cells was reduced after the addition of isolated paraprotein to PRP from healthy donors (p lt 0.001). These investigations demonstrated that paraprotein causes platelet dysfunction in patients with MG due to specific binding to the platelet vWF receptor GPIb and platelet collagen receptor GPVI. Copyright (c) 2013 S. Karger AG, Basel", publisher = "Karger, Basel", journal = "Acta Haematologica", title = "Specific Binding of Paraprotein to Platelet Receptors as a Cause of Platelet Dysfunction in Monoclonal Gammopathies", pages = "107-101", number = "2", volume = "130", doi = "10.1159/000345418" }
Đunić, I., Elezović, I., Vucić, M., Srdić-Rajić, T., Konić-Ristić, A., Ilić, V., Milić, N., Bila, J., Suvajdžić-Vuković, N., Virijević, M., Antić, D., Vidović, A.,& Tomin, D.. (2013). Specific Binding of Paraprotein to Platelet Receptors as a Cause of Platelet Dysfunction in Monoclonal Gammopathies. in Acta Haematologica Karger, Basel., 130(2), 101-107. https://doi.org/10.1159/000345418
Đunić I, Elezović I, Vucić M, Srdić-Rajić T, Konić-Ristić A, Ilić V, Milić N, Bila J, Suvajdžić-Vuković N, Virijević M, Antić D, Vidović A, Tomin D. Specific Binding of Paraprotein to Platelet Receptors as a Cause of Platelet Dysfunction in Monoclonal Gammopathies. in Acta Haematologica. 2013;130(2):101-107. doi:10.1159/000345418 .
Đunić, I., Elezović, Ivo, Vucić, M., Srdić-Rajić, Tatjana, Konić-Ristić, Aleksandra, Ilić, Vesna, Milić, Nataša, Bila, Jelena, Suvajdžić-Vuković, Nada, Virijević, Marijana, Antić, Darko, Vidović, Ana, Tomin, Dragica, "Specific Binding of Paraprotein to Platelet Receptors as a Cause of Platelet Dysfunction in Monoclonal Gammopathies" in Acta Haematologica, 130, no. 2 (2013):101-107, https://doi.org/10.1159/000345418 . .