Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks
Само за регистроване кориснике
2006
Аутори
Radulović, ŽeljkoMilutinović, Marija
Anđelković, Marko
Vujcić, Zoran
Tomanović, Snežana
Božić, Nataša
Marinković, Dragoslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Ixodes ricinus Linnaeus (Acari: Ixodidae) ticks are vectors of numerous infectious diseases in humans and animals. The allozyme variability of MDH and alpha-Gpdh was detected by native polyacrylamide gel electrophoresis in I. ricinus natural populations in three localities in Serbia. Four alleles of Mdh locus (MDH 1, MDH 2, MDH 3 and MDH X) and four alleles of alpha-Gpdh locus (VS, S, F and VF) were detected. Interpopulation differences in Mdh and alpha-Gpdh allele frequencies were statistically insignificant. Significant difference in alpha-Gpdh allele frequencies between mates and females was recorded in the largest sample only. Differences in allele frequencies, detected between borreliae-infected and uninfected I. ricinus ticks, were close to the level of statistical significance, especially for alpha-Gpdh locus. Clear significant difference appeared in females when sexes were tested separatelly (P = 0.037). It is interesting that genotypes containing rarer alleles (MDH 1 and S) we...re infected in higher proportion in comparison to other genotypes. Our results point towards a possible role of Mdh and alpha-Gpdh loci in I. ricinus ticks in the determination of energy requirements for host seeking. Sex differences in alpha-Gpdh allele frequencies suggest that selective pressure, concerning efficiency of reserve materials utilisation, points to alpha-Gpdh rather than to Mdh locus.
Кључне речи:
Ixodes ricinus / Borrelia burgdorferi sensu lato / Lyme disease / allozyme variability / alpha-glycerophosphate dehydrogenase / malate dehydrogenaseИзвор:
Experimental & Applied Acarology, 2006, 40, 2, 113-121Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Инфекције интерцелуларним микроорганизмима растућег значаја: трансмисија, однос патоген-домаћин, молекуларна епидемиологија и клинички значај (RS-MESTD-MPN2006-2010-145002)
DOI: 10.1007/s10493-006-9028-z
ISSN: 0168-8162
PubMed: 17072538
WoS: 000243152700003
Scopus: 2-s2.0-33751290652
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Radulović, Željko AU - Milutinović, Marija AU - Anđelković, Marko AU - Vujcić, Zoran AU - Tomanović, Snežana AU - Božić, Nataša AU - Marinković, Dragoslav PY - 2006 UR - http://rimi.imi.bg.ac.rs/handle/123456789/138 AB - Ixodes ricinus Linnaeus (Acari: Ixodidae) ticks are vectors of numerous infectious diseases in humans and animals. The allozyme variability of MDH and alpha-Gpdh was detected by native polyacrylamide gel electrophoresis in I. ricinus natural populations in three localities in Serbia. Four alleles of Mdh locus (MDH 1, MDH 2, MDH 3 and MDH X) and four alleles of alpha-Gpdh locus (VS, S, F and VF) were detected. Interpopulation differences in Mdh and alpha-Gpdh allele frequencies were statistically insignificant. Significant difference in alpha-Gpdh allele frequencies between mates and females was recorded in the largest sample only. Differences in allele frequencies, detected between borreliae-infected and uninfected I. ricinus ticks, were close to the level of statistical significance, especially for alpha-Gpdh locus. Clear significant difference appeared in females when sexes were tested separatelly (P = 0.037). It is interesting that genotypes containing rarer alleles (MDH 1 and S) were infected in higher proportion in comparison to other genotypes. Our results point towards a possible role of Mdh and alpha-Gpdh loci in I. ricinus ticks in the determination of energy requirements for host seeking. Sex differences in alpha-Gpdh allele frequencies suggest that selective pressure, concerning efficiency of reserve materials utilisation, points to alpha-Gpdh rather than to Mdh locus. PB - Springer, Dordrecht T2 - Experimental & Applied Acarology T1 - Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks EP - 121 IS - 2 SP - 113 VL - 40 DO - 10.1007/s10493-006-9028-z ER -
@article{ author = "Radulović, Željko and Milutinović, Marija and Anđelković, Marko and Vujcić, Zoran and Tomanović, Snežana and Božić, Nataša and Marinković, Dragoslav", year = "2006", abstract = "Ixodes ricinus Linnaeus (Acari: Ixodidae) ticks are vectors of numerous infectious diseases in humans and animals. The allozyme variability of MDH and alpha-Gpdh was detected by native polyacrylamide gel electrophoresis in I. ricinus natural populations in three localities in Serbia. Four alleles of Mdh locus (MDH 1, MDH 2, MDH 3 and MDH X) and four alleles of alpha-Gpdh locus (VS, S, F and VF) were detected. Interpopulation differences in Mdh and alpha-Gpdh allele frequencies were statistically insignificant. Significant difference in alpha-Gpdh allele frequencies between mates and females was recorded in the largest sample only. Differences in allele frequencies, detected between borreliae-infected and uninfected I. ricinus ticks, were close to the level of statistical significance, especially for alpha-Gpdh locus. Clear significant difference appeared in females when sexes were tested separatelly (P = 0.037). It is interesting that genotypes containing rarer alleles (MDH 1 and S) were infected in higher proportion in comparison to other genotypes. Our results point towards a possible role of Mdh and alpha-Gpdh loci in I. ricinus ticks in the determination of energy requirements for host seeking. Sex differences in alpha-Gpdh allele frequencies suggest that selective pressure, concerning efficiency of reserve materials utilisation, points to alpha-Gpdh rather than to Mdh locus.", publisher = "Springer, Dordrecht", journal = "Experimental & Applied Acarology", title = "Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks", pages = "121-113", number = "2", volume = "40", doi = "10.1007/s10493-006-9028-z" }
Radulović, Ž., Milutinović, M., Anđelković, M., Vujcić, Z., Tomanović, S., Božić, N.,& Marinković, D.. (2006). Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks. in Experimental & Applied Acarology Springer, Dordrecht., 40(2), 113-121. https://doi.org/10.1007/s10493-006-9028-z
Radulović Ž, Milutinović M, Anđelković M, Vujcić Z, Tomanović S, Božić N, Marinković D. Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks. in Experimental & Applied Acarology. 2006;40(2):113-121. doi:10.1007/s10493-006-9028-z .
Radulović, Željko, Milutinović, Marija, Anđelković, Marko, Vujcić, Zoran, Tomanović, Snežana, Božić, Nataša, Marinković, Dragoslav, "Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks" in Experimental & Applied Acarology, 40, no. 2 (2006):113-121, https://doi.org/10.1007/s10493-006-9028-z . .