Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties
Samo za registrovane korisnike
2020
Autori
Jauković, AleksandraAbadjieva, Desislava
Trivanović, Drenka
Stoyanova, Elena
Kostadinova, Milena
Pashova, Shina
Kestendjieva, Snejana
Kukolj, Tamara
Ješeta, Michal
Kistanova, Elena
Mourdjeva, Milena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and me...tabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.
Ključne reči:
Mesenchymal stem cells / 3D spheroids microenvironment / Morphological changes / Cell survival / Energy metabolism / Epigenetic regulation / Improved functionalityIzvor:
Stem Cell Reviews & Reports, 2020, 16, 5, 853-875Izdavač:
- Springer, New York
Finansiranje / projekti:
- COST action [CA16119 - Action CellFit]
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200015 (Univerzitet u Beogradu, Institut za medicinska istraživanja) (RS-MESTD-inst-2020-200015)
- Bulgarian National Science Fund, Sofia, Bulgaria [DCOST01/18, KP-06COST/21]
- Ministry of Education, Youth and Sports of the Czech RepublicMinistry of Education, Youth & Sports - Czech Republic [LTC-18059]
DOI: 10.1007/s12015-020-10006-9
ISSN: 2629-3269
PubMed: 32681232
WoS: 000549663600001
Scopus: 2-s2.0-85088108884
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Jauković, Aleksandra AU - Abadjieva, Desislava AU - Trivanović, Drenka AU - Stoyanova, Elena AU - Kostadinova, Milena AU - Pashova, Shina AU - Kestendjieva, Snejana AU - Kukolj, Tamara AU - Ješeta, Michal AU - Kistanova, Elena AU - Mourdjeva, Milena PY - 2020 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1055 AB - Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application. PB - Springer, New York T2 - Stem Cell Reviews & Reports T1 - Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties EP - 875 IS - 5 SP - 853 VL - 16 DO - 10.1007/s12015-020-10006-9 ER -
@article{ author = "Jauković, Aleksandra and Abadjieva, Desislava and Trivanović, Drenka and Stoyanova, Elena and Kostadinova, Milena and Pashova, Shina and Kestendjieva, Snejana and Kukolj, Tamara and Ješeta, Michal and Kistanova, Elena and Mourdjeva, Milena", year = "2020", abstract = "Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.", publisher = "Springer, New York", journal = "Stem Cell Reviews & Reports", title = "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties", pages = "875-853", number = "5", volume = "16", doi = "10.1007/s12015-020-10006-9" }
Jauković, A., Abadjieva, D., Trivanović, D., Stoyanova, E., Kostadinova, M., Pashova, S., Kestendjieva, S., Kukolj, T., Ješeta, M., Kistanova, E.,& Mourdjeva, M.. (2020). Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports Springer, New York., 16(5), 853-875. https://doi.org/10.1007/s12015-020-10006-9
Jauković A, Abadjieva D, Trivanović D, Stoyanova E, Kostadinova M, Pashova S, Kestendjieva S, Kukolj T, Ješeta M, Kistanova E, Mourdjeva M. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports. 2020;16(5):853-875. doi:10.1007/s12015-020-10006-9 .
Jauković, Aleksandra, Abadjieva, Desislava, Trivanović, Drenka, Stoyanova, Elena, Kostadinova, Milena, Pashova, Shina, Kestendjieva, Snejana, Kukolj, Tamara, Ješeta, Michal, Kistanova, Elena, Mourdjeva, Milena, "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties" in Stem Cell Reviews & Reports, 16, no. 5 (2020):853-875, https://doi.org/10.1007/s12015-020-10006-9 . .