TY  - JOUR
AU  - Rajković, Katarina M.
AU  - Drobac, Milica
AU  - Milić, Petar
AU  - Vučić, Vesna
AU  - Arsić, Aleksandra
AU  - Perić, Mirijana
AU  - Radunović, Milena
AU  - Jeremić, Sanja
AU  - Arsenijević, Jelena
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1322
AB  - Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n- -6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk
EP  - 614
IS  - 6
SP  - 603
VL  - 88
DO  - 10.2298/JSC230210024R
ER  - 

@article{
author = "Rajković, Katarina M. and Drobac, Milica and Milić, Petar and Vučić, Vesna and Arsić, Aleksandra and Perić, Mirijana and Radunović, Milena and Jeremić, Sanja and Arsenijević, Jelena",
year = "2023",
abstract = "Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n- -6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society, Journal of the Serbian Chemical Society",
title = "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk",
pages = "614-603",
number = "6",
volume = "88",
doi = "10.2298/JSC230210024R"
}

Rajković, K. M., Drobac, M., Milić, P., Vučić, V., Arsić, A., Perić, M., Radunović, M., Jeremić, S.,& Arsenijević, J.. (2023). Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 88(6), 603-614.
https://doi.org/10.2298/JSC230210024R

Rajković KM, Drobac M, Milić P, Vučić V, Arsić A, Perić M, Radunović M, Jeremić S, Arsenijević J. Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society. 2023;88(6):603-614.
doi:10.2298/JSC230210024R .

Rajković, Katarina M., Drobac, Milica, Milić, Petar, Vučić, Vesna, Arsić, Aleksandra, Perić, Mirijana, Radunović, Milena, Jeremić, Sanja, Arsenijević, Jelena, "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk" in Journal of the Serbian Chemical Society, 88, no. 6 (2023):603-614,
https://doi.org/10.2298/JSC230210024R . .