TY  - JOUR
AU  - Benjumeda-Wijnhoven, Isabel M.
AU  - Vallejos, Raul
AU  - Santibanez, Juan F.
AU  - Millán, Carola S.
AU  - Vivanco, Juan F.
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/992
AB  - The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.
PB  - Nature Research, Berlin
T2  - Scientific Reports
T1  - Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics
IS  - 1
SP  - 16493
VL  - 10
DO  - 10.1038/s41598-020-73428-y
ER  - 

@article{
author = "Benjumeda-Wijnhoven, Isabel M. and Vallejos, Raul and Santibanez, Juan F. and Millán, Carola S. and Vivanco, Juan F.",
year = "2020",
abstract = "The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.",
publisher = "Nature Research, Berlin",
journal = "Scientific Reports",
title = "Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics",
number = "1",
pages = "16493",
volume = "10",
doi = "10.1038/s41598-020-73428-y"
}

Benjumeda-Wijnhoven, I. M., Vallejos, R., Santibanez, J. F., Millán, C. S.,& Vivanco, J. F.. (2020). Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics. in Scientific Reports
Nature Research, Berlin., 10(1), 16493.
https://doi.org/10.1038/s41598-020-73428-y

Benjumeda-Wijnhoven IM, Vallejos R, Santibanez JF, Millán CS, Vivanco JF. Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics. in Scientific Reports. 2020;10(1):16493.
doi:10.1038/s41598-020-73428-y .

Benjumeda-Wijnhoven, Isabel M., Vallejos, Raul, Santibanez, Juan F., Millán, Carola S., Vivanco, Juan F., "Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics" in Scientific Reports, 10, no. 1 (2020):16493,
https://doi.org/10.1038/s41598-020-73428-y . .

TY  - JOUR
AU  - Millan, Carola
AU  - Vivanco, Juan F.
AU  - Benjumeda-Wijnhoven, Isabel M.
AU  - Bjelica, Sunčica
AU  - Santibanez, Juan F.
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/863
AB  - In orthopedic medicine, a feasible reconstruction of bone structures remains one of the main challenges both for healthcare and for improvement of patients' quality of life. There is a growing interest in mesenchymal stem cells (MSCs) medical application, due to their multilineage differentiation potential, and tissue engineering integration to improve bone repair and regeneration. In this review we will describe the main characteristics of MSCs, such as osteogenesis, immunomodulation and antibacterial properties, key parameters to consider during bone repair strategies. Moreover, we describe the properties of calciumphosphate (CaP) bioceramics, which demonstrate to be useful tools in combination with MSCs, due to their biocompatibility, osseointegration and osteoconduction for bone repair and regeneration. Also, we overview the main characteristics of dental cavity MSCs, which are promising candidates, in combination with CaP bioceramics, for bone regeneration and tissue engineering. The understanding of MSCs biology and their interaction with CaP bioceramics and other biomaterials is critical for orthopedic surgical bone replacement, reconstruction and regeneration, which is an integrative and dynamic medical, scientific and bioengineering field of research and biotechnology.
PB  - Springer International Publishing Ag, Cham
T2  - Cell Biology & Translational Medicine, Vol 3: Stem Cells, Bio-Materials & Tissue Engineering
T1  - Mesenchymal Stem Cells and Calcium Phosphate Bioceramics: Implications in Periodontal Bone Regeneration
EP  - 112
SP  - 91
VL  - 1107
DO  - 10.1007/5584_2018_249
ER  - 

@article{
author = "Millan, Carola and Vivanco, Juan F. and Benjumeda-Wijnhoven, Isabel M. and Bjelica, Sunčica and Santibanez, Juan F.",
year = "2018",
abstract = "In orthopedic medicine, a feasible reconstruction of bone structures remains one of the main challenges both for healthcare and for improvement of patients' quality of life. There is a growing interest in mesenchymal stem cells (MSCs) medical application, due to their multilineage differentiation potential, and tissue engineering integration to improve bone repair and regeneration. In this review we will describe the main characteristics of MSCs, such as osteogenesis, immunomodulation and antibacterial properties, key parameters to consider during bone repair strategies. Moreover, we describe the properties of calciumphosphate (CaP) bioceramics, which demonstrate to be useful tools in combination with MSCs, due to their biocompatibility, osseointegration and osteoconduction for bone repair and regeneration. Also, we overview the main characteristics of dental cavity MSCs, which are promising candidates, in combination with CaP bioceramics, for bone regeneration and tissue engineering. The understanding of MSCs biology and their interaction with CaP bioceramics and other biomaterials is critical for orthopedic surgical bone replacement, reconstruction and regeneration, which is an integrative and dynamic medical, scientific and bioengineering field of research and biotechnology.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Cell Biology & Translational Medicine, Vol 3: Stem Cells, Bio-Materials & Tissue Engineering",
title = "Mesenchymal Stem Cells and Calcium Phosphate Bioceramics: Implications in Periodontal Bone Regeneration",
pages = "112-91",
volume = "1107",
doi = "10.1007/5584_2018_249"
}

Millan, C., Vivanco, J. F., Benjumeda-Wijnhoven, I. M., Bjelica, S.,& Santibanez, J. F.. (2018). Mesenchymal Stem Cells and Calcium Phosphate Bioceramics: Implications in Periodontal Bone Regeneration. in Cell Biology & Translational Medicine, Vol 3: Stem Cells, Bio-Materials & Tissue Engineering
Springer International Publishing Ag, Cham., 1107, 91-112.
https://doi.org/10.1007/5584_2018_249

Millan C, Vivanco JF, Benjumeda-Wijnhoven IM, Bjelica S, Santibanez JF. Mesenchymal Stem Cells and Calcium Phosphate Bioceramics: Implications in Periodontal Bone Regeneration. in Cell Biology & Translational Medicine, Vol 3: Stem Cells, Bio-Materials & Tissue Engineering. 2018;1107:91-112.
doi:10.1007/5584_2018_249 .

Millan, Carola, Vivanco, Juan F., Benjumeda-Wijnhoven, Isabel M., Bjelica, Sunčica, Santibanez, Juan F., "Mesenchymal Stem Cells and Calcium Phosphate Bioceramics: Implications in Periodontal Bone Regeneration" in Cell Biology & Translational Medicine, Vol 3: Stem Cells, Bio-Materials & Tissue Engineering, 1107 (2018):91-112,
https://doi.org/10.1007/5584_2018_249 . .