TY  - JOUR
AU  - Paraš, Smiljana
AU  - Trišić, Dijana
AU  - Mitrović-Ajtić, Olivera
AU  - Prokić, Bogomir
AU  - Drobne, Damjana
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1062
AB  - Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.
PB  - MDPI, Basel
T2  - Nanomaterials
T1  - Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats
IS  - 5
SP  - 918
VL  - 10
DO  - 10.3390/nano10050918
ER  - 

@article{
author = "Paraš, Smiljana and Trišić, Dijana and Mitrović-Ajtić, Olivera and Prokić, Bogomir and Drobne, Damjana and Živković, Slavoljub and Jokanović, Vukoman",
year = "2020",
abstract = "Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.",
publisher = "MDPI, Basel",
journal = "Nanomaterials",
title = "Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats",
number = "5",
pages = "918",
volume = "10",
doi = "10.3390/nano10050918"
}

Paraš, S., Trišić, D., Mitrović-Ajtić, O., Prokić, B., Drobne, D., Živković, S.,& Jokanović, V.. (2020). Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats. in Nanomaterials
MDPI, Basel., 10(5), 918.
https://doi.org/10.3390/nano10050918

Paraš S, Trišić D, Mitrović-Ajtić O, Prokić B, Drobne D, Živković S, Jokanović V. Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats. in Nanomaterials. 2020;10(5):918.
doi:10.3390/nano10050918 .

Paraš, Smiljana, Trišić, Dijana, Mitrović-Ajtić, Olivera, Prokić, Bogomir, Drobne, Damjana, Živković, Slavoljub, Jokanović, Vukoman, "Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats" in Nanomaterials, 10, no. 5 (2020):918,
https://doi.org/10.3390/nano10050918 . .

TY  - JOUR
AU  - Prokić, Bogomir
AU  - Todorović, Vera
AU  - Mitrović, Olivera
AU  - Vignjević, Sanja
AU  - Savić-Stevanović, Vera
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/308
AB  - Megacolon refers to an abnormal dilatation of the colon. This condition occurs in both humans and animals. Although it seems to be more common in cats, megacolon may also occur in dogs. However, data regarding the etiopathogenesis, clinical course and outcome of canine megacolon are scarce. The aim of this study is to present the experience of our team in diagnosis and therapy of canine acquired megacolon, with particular reference to etiopathogenetic aspects. The prospective study included 28 dogs affected with megacolon, aged 5-9 years. The 26 animals underwent a surgical procedure (colonotomy followed by manual extraction of faeces), and were followed up for a period of 28 days. On the basis of anamnestic data, clinical and radiographic findings, 7 dogs (25%) were presented with idiophatic acquired megacolon, while 75% of cases had secundary acquired megacolon of different etiology (including pelvic canal stenosis, lumbar and sacral spinal injuries or back leg fractures, in 46% of cases; keeping the animals in the backyard and irresponsibility of their owners, in 11%; non-adequate nutrition, in 11%; and decreased physical activity and keeping animals in small flats, in 7%). During early postoperative period, the medical treatment and dietary regimen enabled defecation in 65% of cases. The remaining 35% of cases were treated with Cisapride in order to establish spontaneous defecation. All dogs recovered completely during the 28- days follow-up period. According to the results of interviews with dog owners, all animals were in good condition six months after the surgical procedure.
AB  - Megakolon označava abnormalnu dilataciju kolona. Javlja se i kod ljudi i kod životinja. Iako je oboljenje češće kod mačaka, javlja se i kod pasa. Međutim, podaci o etiopatogenezi, kliničkom toku i ishodu bolesti megakolona kod pasa su oskudni. Stoga je cilj ove studije da se iznesu sopstvena iskustva o dijagnostici i terapiji stečenog megakolona kod pasa, sa posebnim naglaskom na etiopatogenetski aspekt oboljenja. Studija je bila prospektivnog karaktera i obuhvatila je 28 pasa sa megakolonom, starosti 5-9 godina. Dvadeset šest životinja podvrgnuto je hirurškoj intervenciji (kolonotomija sa manuelnom ekstrakcijom fecesa) i praćeno postoperativno u toku 28 dana. Na osnovu anamnestičkih podataka dobijenih od vlasnika pasa, kliničkog i radiografskog nalaza, 7 pasa (25%) imalo je idiopatski stečeni megakolon, dok je kod 21 psa (75%) bolest okarakterisana kao sekundarni stečeni megakolon uzrokovan različitim etiološkim faktorima (uključujući stenozu pelvičnog kanala, oštećenje lumbalne i sakralne kičme ili frakturu zadnjih ekstremiteta, kod 46% pasa; čuvanje životinja u dvorištu i nedovoljni nadzor od strane vlasnika, kod 11%; neadekvatna ishrana, kod 11%; smanjenja fizičke aktivnosti i čuvanje životinja u skučenom prostoru, kod 7%). U ranom postoperativnom periodu, terapija i režim ishrane doveli su do uspostavljanja defekacije kod 65% pasa. Kod preostalih 35% slučajeva spontana defekacija je uspostavljena primenom terapije Cisapridom. Sve životinje su se u toku 28-dnevnog nadzora kompletno oporavile, a odlično opšte stanje zabeleženo je, na osnovu intervjua sa vlasnicima i 6 meseci nakon hirurške intervencije.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Ethiopathogenesis, diagnosis and therapy of acquired megacolon in dogs
T1  - Etiopatogeneza, dijagnoza i terapija stečenog megakolona pasa
EP  - 284
IS  - 2-3
SP  - 273
VL  - 60
DO  - 10.2298/AVB1003273P
ER  - 

@article{
author = "Prokić, Bogomir and Todorović, Vera and Mitrović, Olivera and Vignjević, Sanja and Savić-Stevanović, Vera",
year = "2010",
abstract = "Megacolon refers to an abnormal dilatation of the colon. This condition occurs in both humans and animals. Although it seems to be more common in cats, megacolon may also occur in dogs. However, data regarding the etiopathogenesis, clinical course and outcome of canine megacolon are scarce. The aim of this study is to present the experience of our team in diagnosis and therapy of canine acquired megacolon, with particular reference to etiopathogenetic aspects. The prospective study included 28 dogs affected with megacolon, aged 5-9 years. The 26 animals underwent a surgical procedure (colonotomy followed by manual extraction of faeces), and were followed up for a period of 28 days. On the basis of anamnestic data, clinical and radiographic findings, 7 dogs (25%) were presented with idiophatic acquired megacolon, while 75% of cases had secundary acquired megacolon of different etiology (including pelvic canal stenosis, lumbar and sacral spinal injuries or back leg fractures, in 46% of cases; keeping the animals in the backyard and irresponsibility of their owners, in 11%; non-adequate nutrition, in 11%; and decreased physical activity and keeping animals in small flats, in 7%). During early postoperative period, the medical treatment and dietary regimen enabled defecation in 65% of cases. The remaining 35% of cases were treated with Cisapride in order to establish spontaneous defecation. All dogs recovered completely during the 28- days follow-up period. According to the results of interviews with dog owners, all animals were in good condition six months after the surgical procedure., Megakolon označava abnormalnu dilataciju kolona. Javlja se i kod ljudi i kod životinja. Iako je oboljenje češće kod mačaka, javlja se i kod pasa. Međutim, podaci o etiopatogenezi, kliničkom toku i ishodu bolesti megakolona kod pasa su oskudni. Stoga je cilj ove studije da se iznesu sopstvena iskustva o dijagnostici i terapiji stečenog megakolona kod pasa, sa posebnim naglaskom na etiopatogenetski aspekt oboljenja. Studija je bila prospektivnog karaktera i obuhvatila je 28 pasa sa megakolonom, starosti 5-9 godina. Dvadeset šest životinja podvrgnuto je hirurškoj intervenciji (kolonotomija sa manuelnom ekstrakcijom fecesa) i praćeno postoperativno u toku 28 dana. Na osnovu anamnestičkih podataka dobijenih od vlasnika pasa, kliničkog i radiografskog nalaza, 7 pasa (25%) imalo je idiopatski stečeni megakolon, dok je kod 21 psa (75%) bolest okarakterisana kao sekundarni stečeni megakolon uzrokovan različitim etiološkim faktorima (uključujući stenozu pelvičnog kanala, oštećenje lumbalne i sakralne kičme ili frakturu zadnjih ekstremiteta, kod 46% pasa; čuvanje životinja u dvorištu i nedovoljni nadzor od strane vlasnika, kod 11%; neadekvatna ishrana, kod 11%; smanjenja fizičke aktivnosti i čuvanje životinja u skučenom prostoru, kod 7%). U ranom postoperativnom periodu, terapija i režim ishrane doveli su do uspostavljanja defekacije kod 65% pasa. Kod preostalih 35% slučajeva spontana defekacija je uspostavljena primenom terapije Cisapridom. Sve životinje su se u toku 28-dnevnog nadzora kompletno oporavile, a odlično opšte stanje zabeleženo je, na osnovu intervjua sa vlasnicima i 6 meseci nakon hirurške intervencije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Ethiopathogenesis, diagnosis and therapy of acquired megacolon in dogs, Etiopatogeneza, dijagnoza i terapija stečenog megakolona pasa",
pages = "284-273",
number = "2-3",
volume = "60",
doi = "10.2298/AVB1003273P"
}

Prokić, B., Todorović, V., Mitrović, O., Vignjević, S.,& Savić-Stevanović, V.. (2010). Ethiopathogenesis, diagnosis and therapy of acquired megacolon in dogs. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 273-284.
https://doi.org/10.2298/AVB1003273P

Prokić B, Todorović V, Mitrović O, Vignjević S, Savić-Stevanović V. Ethiopathogenesis, diagnosis and therapy of acquired megacolon in dogs. in Acta Veterinaria-Beograd. 2010;60(2-3):273-284.
doi:10.2298/AVB1003273P .

Prokić, Bogomir, Todorović, Vera, Mitrović, Olivera, Vignjević, Sanja, Savić-Stevanović, Vera, "Ethiopathogenesis, diagnosis and therapy of acquired megacolon in dogs" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):273-284,
https://doi.org/10.2298/AVB1003273P . .

TY  - JOUR
AU  - Prokić, Bogomir
AU  - Todorović, Vera
AU  - Drndarević, Neda
AU  - Vignjević, Sanja
AU  - Đikić, Dragoslava
AU  - Nikolić, I.
AU  - Marinković, S.
AU  - Slavik, E.
PY  - 2009
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/243
AB  - It is well established that megacolon in carnivores, including both cats and dogs, is a common finding. Megacolon occurs more often in the cat that the dog. Based on current data idiopathic megacolon is a common cause of constipation in cats (62% of constipated cats are affected by diopathic megacolon). There is no evidence of idiopathic megacolon in dogs and publications about this disease in this species is very scarce. We investigated the enteric nervous system in the dilated portion (DP) of the colon in dogs with idiopathic aquired (n=7) or secondary aquired megacolon (n=21) and compared the results with a normal colon in control dogs (n=3). Colonic sections of surgical specimens were investigated by conventional and immunohistochemical methods, including pan-neuronal markers (NSE, synaptophisin, and neurofilament) and VIP, as well as S-100 protein for detection of ganglionic glial cells. Compared to controls, the two megacolon groups showed no changes of density of enteric neurons in both submucosal and myenteric nervous plexuses in DP of the colon and of enteric glial cells. However, compared to controls and dogs with secondary megacolon, there was a significant decrease in the density of NFP-ir nerve fibers in the longitudinal muscle layer in dogs with idiopathic acquired megacolon. In addition, dogs with idiopathic megacolon display decreased VIP-ir in the myenteric plexus and lamina propria mucosae, and absence of VIP-ir neurons in the submucosal plexus of DP of the colon. Similar alterations, although of lesser severity, may be found in dogs with secondary aquired megacolon. We consider that both idiopathic and secondary aquired megacolon might occur on the basis of a dysplastic changes of VIP-ir enteric neurons.
AB  - Poznato je da se magakolon javlja kod mesojeda, uključujući mačke i pse, pri čemu je ovo oboljenje daleko učestalije kod mačaka. Na osnovu dosadašnjih saznanja, idiopatski megakolon je čest uzročnik konstipacije kod mačaka i 62% mačaka sa konstipacijom ima idiopatski megakolon. Istovremeno, podaci o psima sa idiopatskim megakolonom veoma su oskudni. U ovom radu je proučavan enterični nervni sistem u dilatiranom delu kolona kod 7 pasa sa idiopatskim megakolonom i 21 psa sa sekundarnim stečenim megakolonom, a rezultati su upoređeni sa normalnim kolonom kod 3 kontrolne zdrave životinje. Tkivni preseci kolona bojeni su klasičnim histološkim i imunohistohemijskim metodama, pri čemu su primenjeni pan-neuronski markeri (NSE, sinaptofizin i neurofilament) i VIP, kao i S-100 protein za detekciju glijalnih ćelija u enteričnim ganglijama. Nisu otkrivene razlike u gustini enteričnih neurona u submukoznom i mijenteričnom pleksusu kod životinja sa megakolonom, kao ni razlike u gustini glijalnih ćelija enteričnih ganglija, u odnosu na kontrolnu grupu životinja. Međutim, u odnosu na kontrolnu grupu, kod životinja sa idiopatskim megakolonom dokazana je smanjena VIP-imunoreaktivnost (ir) u mienteričnom pleksusu i krznu mukoze, kao i kompletno odsustvo VIP-ir neurona u submukoznom pleksusu dilatiranog dela kolona. Slične promene, ali u manjem stepenu, postojale su kod pasa sa sekundarnim stečenim megakolonom. Može da se zaključi da u patogenezi idiopatskog i sekundarnog stečenog megakolona značajnu ulogu imaju displastične promene u VIP-ergičkim neuronima enteričkog nervnog sistema.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Idiopathic and secondary acquired megacolon in dogs is associated with diminished vasoactive intestinal polypeptide innervation of the affected colon
T1  - Idiopatski i sekundarni stečeni megakolon kod pasa udruženi su sa smanjenom vip-inervacijom u oštećenom kolonu
EP  - 68
IS  - 1
SP  - 53
VL  - 59
DO  - 10.2298/AVB0901053P
ER  - 

@article{
author = "Prokić, Bogomir and Todorović, Vera and Drndarević, Neda and Vignjević, Sanja and Đikić, Dragoslava and Nikolić, I. and Marinković, S. and Slavik, E.",
year = "2009",
abstract = "It is well established that megacolon in carnivores, including both cats and dogs, is a common finding. Megacolon occurs more often in the cat that the dog. Based on current data idiopathic megacolon is a common cause of constipation in cats (62% of constipated cats are affected by diopathic megacolon). There is no evidence of idiopathic megacolon in dogs and publications about this disease in this species is very scarce. We investigated the enteric nervous system in the dilated portion (DP) of the colon in dogs with idiopathic aquired (n=7) or secondary aquired megacolon (n=21) and compared the results with a normal colon in control dogs (n=3). Colonic sections of surgical specimens were investigated by conventional and immunohistochemical methods, including pan-neuronal markers (NSE, synaptophisin, and neurofilament) and VIP, as well as S-100 protein for detection of ganglionic glial cells. Compared to controls, the two megacolon groups showed no changes of density of enteric neurons in both submucosal and myenteric nervous plexuses in DP of the colon and of enteric glial cells. However, compared to controls and dogs with secondary megacolon, there was a significant decrease in the density of NFP-ir nerve fibers in the longitudinal muscle layer in dogs with idiopathic acquired megacolon. In addition, dogs with idiopathic megacolon display decreased VIP-ir in the myenteric plexus and lamina propria mucosae, and absence of VIP-ir neurons in the submucosal plexus of DP of the colon. Similar alterations, although of lesser severity, may be found in dogs with secondary aquired megacolon. We consider that both idiopathic and secondary aquired megacolon might occur on the basis of a dysplastic changes of VIP-ir enteric neurons., Poznato je da se magakolon javlja kod mesojeda, uključujući mačke i pse, pri čemu je ovo oboljenje daleko učestalije kod mačaka. Na osnovu dosadašnjih saznanja, idiopatski megakolon je čest uzročnik konstipacije kod mačaka i 62% mačaka sa konstipacijom ima idiopatski megakolon. Istovremeno, podaci o psima sa idiopatskim megakolonom veoma su oskudni. U ovom radu je proučavan enterični nervni sistem u dilatiranom delu kolona kod 7 pasa sa idiopatskim megakolonom i 21 psa sa sekundarnim stečenim megakolonom, a rezultati su upoređeni sa normalnim kolonom kod 3 kontrolne zdrave životinje. Tkivni preseci kolona bojeni su klasičnim histološkim i imunohistohemijskim metodama, pri čemu su primenjeni pan-neuronski markeri (NSE, sinaptofizin i neurofilament) i VIP, kao i S-100 protein za detekciju glijalnih ćelija u enteričnim ganglijama. Nisu otkrivene razlike u gustini enteričnih neurona u submukoznom i mijenteričnom pleksusu kod životinja sa megakolonom, kao ni razlike u gustini glijalnih ćelija enteričnih ganglija, u odnosu na kontrolnu grupu životinja. Međutim, u odnosu na kontrolnu grupu, kod životinja sa idiopatskim megakolonom dokazana je smanjena VIP-imunoreaktivnost (ir) u mienteričnom pleksusu i krznu mukoze, kao i kompletno odsustvo VIP-ir neurona u submukoznom pleksusu dilatiranog dela kolona. Slične promene, ali u manjem stepenu, postojale su kod pasa sa sekundarnim stečenim megakolonom. Može da se zaključi da u patogenezi idiopatskog i sekundarnog stečenog megakolona značajnu ulogu imaju displastične promene u VIP-ergičkim neuronima enteričkog nervnog sistema.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Idiopathic and secondary acquired megacolon in dogs is associated with diminished vasoactive intestinal polypeptide innervation of the affected colon, Idiopatski i sekundarni stečeni megakolon kod pasa udruženi su sa smanjenom vip-inervacijom u oštećenom kolonu",
pages = "68-53",
number = "1",
volume = "59",
doi = "10.2298/AVB0901053P"
}

Prokić, B., Todorović, V., Drndarević, N., Vignjević, S., Đikić, D., Nikolić, I., Marinković, S.,& Slavik, E.. (2009). Idiopathic and secondary acquired megacolon in dogs is associated with diminished vasoactive intestinal polypeptide innervation of the affected colon. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(1), 53-68.
https://doi.org/10.2298/AVB0901053P

Prokić B, Todorović V, Drndarević N, Vignjević S, Đikić D, Nikolić I, Marinković S, Slavik E. Idiopathic and secondary acquired megacolon in dogs is associated with diminished vasoactive intestinal polypeptide innervation of the affected colon. in Acta Veterinaria-Beograd. 2009;59(1):53-68.
doi:10.2298/AVB0901053P .

Prokić, Bogomir, Todorović, Vera, Drndarević, Neda, Vignjević, Sanja, Đikić, Dragoslava, Nikolić, I., Marinković, S., Slavik, E., "Idiopathic and secondary acquired megacolon in dogs is associated with diminished vasoactive intestinal polypeptide innervation of the affected colon" in Acta Veterinaria-Beograd, 59, no. 1 (2009):53-68,
https://doi.org/10.2298/AVB0901053P . .