Приказ основних података о документу

dc.creatorĆirković, Vladimir
dc.creatorKlun, Ivana
dc.creatorUtaaker, Kjersti Selstad
dc.creatorUzelac, Aleksandra
dc.creatorTysnes, Kristoffer R.
dc.creatorRobertson, Lucy J.
dc.creatorĐurković-Đaković, Olgica
dc.date.accessioned2021-04-20T13:06:31Z
dc.date.available2021-04-20T13:06:31Z
dc.date.issued2020
dc.identifier.issn0014-4894
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/999
dc.description.abstractGiardiasis and cryptosporidiosis are recognized by the WHO as important emerging diseases of the 21st century. Symptoms are similar and include diarrhoea and vomiting, which may be severe, even life-threatening, for the immunocompromised and children under five years of age. Between 2013 and 2017, the Institute for Public Health in Serbia recorded 10 waterborne epidemics that manifested as gastrointestinal disease. Routine testing for enteropathogenic bacteria and viruses did not identify the aetiological agents of these outbreaks. As water is not examined for the presence of protozoa in Serbia, we performed a pilot study to analyse samples from four major rivers and their tributaries using a newly implemented methodology for detection of Giardia and Cryptosporidium, based on the ISO 15553:2006 standard. Using immunofluorescence microscopy, Giardia was detected in 10 out of the 31 samples, Cryptosporidium in five, while two samples were positive for both. Presence of G. duodenalis gDNA was confirmed by amplification of the beta-giardin gene in eight samples, of which one and two, respectively, were identified by RFLP as potentially zoonotic assemblages A and B. The results suggest that surface water in Serbia may be a potential source of infection and call for more in-depth studies using sophisticated molecular tools.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationCOST action [FA1408], A European Network for Food borne Parasites (Euro-FBP)
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41019/RS//
dc.rightsrestrictedAccess
dc.sourceExperimental Parasitology
dc.subjectGiardiaen
dc.subjectCryptosporidiumen
dc.subjectWaterborneen
dc.subjectProtozoaen
dc.subjectDetectionen
dc.subjectSurface wateren
dc.titleSurface waters as a potential source of Giardia and Cryptosporidium in Serbiaen
dc.typearticle
dc.rights.licenseARR
dc.citation.other209: -
dc.citation.rankM22~
dc.citation.spage107824
dc.citation.volume209
dc.identifier.doi10.1016/j.exppara.2019.107824
dc.identifier.pmid31870927
dc.identifier.scopus2-s2.0-85076928215
dc.identifier.wos000510313400001
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу