Surface waters as a potential source of Giardia and Cryptosporidium in Serbia
Само за регистроване кориснике
2020
Аутори
Ćirković, VladimirKlun, Ivana
Utaaker, Kjersti Selstad
Uzelac, Aleksandra
Tysnes, Kristoffer R.
Robertson, Lucy J.
Đurković-Đaković, Olgica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Giardiasis and cryptosporidiosis are recognized by the WHO as important emerging diseases of the 21st century. Symptoms are similar and include diarrhoea and vomiting, which may be severe, even life-threatening, for the immunocompromised and children under five years of age. Between 2013 and 2017, the Institute for Public Health in Serbia recorded 10 waterborne epidemics that manifested as gastrointestinal disease. Routine testing for enteropathogenic bacteria and viruses did not identify the aetiological agents of these outbreaks. As water is not examined for the presence of protozoa in Serbia, we performed a pilot study to analyse samples from four major rivers and their tributaries using a newly implemented methodology for detection of Giardia and Cryptosporidium, based on the ISO 15553:2006 standard. Using immunofluorescence microscopy, Giardia was detected in 10 out of the 31 samples, Cryptosporidium in five, while two samples were positive for both. Presence of G. duodenalis gDNA... was confirmed by amplification of the beta-giardin gene in eight samples, of which one and two, respectively, were identified by RFLP as potentially zoonotic assemblages A and B. The results suggest that surface water in Serbia may be a potential source of infection and call for more in-depth studies using sophisticated molecular tools.
Кључне речи:
Giardia / Cryptosporidium / Waterborne / Protozoa / Detection / Surface waterИзвор:
Experimental Parasitology, 2020, 209, 107824-Издавач:
- Academic Press Inc Elsevier Science, San Diego
Финансирање / пројекти:
- COST action [FA1408], A European Network for Food borne Parasites (Euro-FBP)
- Контрола инфекција апикомплексним патогенима: од нових места деловања лека до предикције (RS-41019)
DOI: 10.1016/j.exppara.2019.107824
ISSN: 0014-4894
PubMed: 31870927
WoS: 000510313400001
Scopus: 2-s2.0-85076928215
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Ćirković, Vladimir AU - Klun, Ivana AU - Utaaker, Kjersti Selstad AU - Uzelac, Aleksandra AU - Tysnes, Kristoffer R. AU - Robertson, Lucy J. AU - Đurković-Đaković, Olgica PY - 2020 UR - http://rimi.imi.bg.ac.rs/handle/123456789/999 AB - Giardiasis and cryptosporidiosis are recognized by the WHO as important emerging diseases of the 21st century. Symptoms are similar and include diarrhoea and vomiting, which may be severe, even life-threatening, for the immunocompromised and children under five years of age. Between 2013 and 2017, the Institute for Public Health in Serbia recorded 10 waterborne epidemics that manifested as gastrointestinal disease. Routine testing for enteropathogenic bacteria and viruses did not identify the aetiological agents of these outbreaks. As water is not examined for the presence of protozoa in Serbia, we performed a pilot study to analyse samples from four major rivers and their tributaries using a newly implemented methodology for detection of Giardia and Cryptosporidium, based on the ISO 15553:2006 standard. Using immunofluorescence microscopy, Giardia was detected in 10 out of the 31 samples, Cryptosporidium in five, while two samples were positive for both. Presence of G. duodenalis gDNA was confirmed by amplification of the beta-giardin gene in eight samples, of which one and two, respectively, were identified by RFLP as potentially zoonotic assemblages A and B. The results suggest that surface water in Serbia may be a potential source of infection and call for more in-depth studies using sophisticated molecular tools. PB - Academic Press Inc Elsevier Science, San Diego T2 - Experimental Parasitology T1 - Surface waters as a potential source of Giardia and Cryptosporidium in Serbia SP - 107824 VL - 209 DO - 10.1016/j.exppara.2019.107824 ER -
@article{ author = "Ćirković, Vladimir and Klun, Ivana and Utaaker, Kjersti Selstad and Uzelac, Aleksandra and Tysnes, Kristoffer R. and Robertson, Lucy J. and Đurković-Đaković, Olgica", year = "2020", abstract = "Giardiasis and cryptosporidiosis are recognized by the WHO as important emerging diseases of the 21st century. Symptoms are similar and include diarrhoea and vomiting, which may be severe, even life-threatening, for the immunocompromised and children under five years of age. Between 2013 and 2017, the Institute for Public Health in Serbia recorded 10 waterborne epidemics that manifested as gastrointestinal disease. Routine testing for enteropathogenic bacteria and viruses did not identify the aetiological agents of these outbreaks. As water is not examined for the presence of protozoa in Serbia, we performed a pilot study to analyse samples from four major rivers and their tributaries using a newly implemented methodology for detection of Giardia and Cryptosporidium, based on the ISO 15553:2006 standard. Using immunofluorescence microscopy, Giardia was detected in 10 out of the 31 samples, Cryptosporidium in five, while two samples were positive for both. Presence of G. duodenalis gDNA was confirmed by amplification of the beta-giardin gene in eight samples, of which one and two, respectively, were identified by RFLP as potentially zoonotic assemblages A and B. The results suggest that surface water in Serbia may be a potential source of infection and call for more in-depth studies using sophisticated molecular tools.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Experimental Parasitology", title = "Surface waters as a potential source of Giardia and Cryptosporidium in Serbia", pages = "107824", volume = "209", doi = "10.1016/j.exppara.2019.107824" }
Ćirković, V., Klun, I., Utaaker, K. S., Uzelac, A., Tysnes, K. R., Robertson, L. J.,& Đurković-Đaković, O.. (2020). Surface waters as a potential source of Giardia and Cryptosporidium in Serbia. in Experimental Parasitology Academic Press Inc Elsevier Science, San Diego., 209, 107824. https://doi.org/10.1016/j.exppara.2019.107824
Ćirković V, Klun I, Utaaker KS, Uzelac A, Tysnes KR, Robertson LJ, Đurković-Đaković O. Surface waters as a potential source of Giardia and Cryptosporidium in Serbia. in Experimental Parasitology. 2020;209:107824. doi:10.1016/j.exppara.2019.107824 .
Ćirković, Vladimir, Klun, Ivana, Utaaker, Kjersti Selstad, Uzelac, Aleksandra, Tysnes, Kristoffer R., Robertson, Lucy J., Đurković-Đaković, Olgica, "Surface waters as a potential source of Giardia and Cryptosporidium in Serbia" in Experimental Parasitology, 209 (2020):107824, https://doi.org/10.1016/j.exppara.2019.107824 . .