The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP ...bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.
Source:
Scientific Reports, 2020, 10, 1, 16493-
Publisher:
Nature Research, Berlin
Funding / projects:
National Commission for Scientific & Technological Research (CONICYT) from the Government of Chile, through the Research Project FONDECYT Inicio [11170957]
PROYECTO NUEVA INGENIERIA 2030 CORFO [14ENI2-26865]
Universidad Adolfo Ibanez
PIA-CONICYT ANILLOComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT PIA/ANILLOS [ACT 17203]
TY - JOUR
AU - Benjumeda-Wijnhoven, Isabel M.
AU - Vallejos, Raul
AU - Santibanez, Juan
AU - Millán, Carola S.
AU - Vivanco, Juan F.
PY - 2020
UR - http://rimi.imi.bg.ac.rs/handle/123456789/992
AB - The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.
PB - Nature Research, Berlin
T2 - Scientific Reports
T1 - Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics
IS - 1
SP - 16493
VL - 10
DO - 10.1038/s41598-020-73428-y
ER -
@article{
author = "Benjumeda-Wijnhoven, Isabel M. and Vallejos, Raul and Santibanez, Juan and Millán, Carola S. and Vivanco, Juan F.",
year = "2020",
abstract = "The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.",
publisher = "Nature Research, Berlin",
journal = "Scientific Reports",
title = "Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics",
number = "1",
pages = "16493",
volume = "10",
doi = "10.1038/s41598-020-73428-y"
}
Benjumeda-Wijnhoven, I. M., Vallejos, R., Santibanez, J., Millán, C. S.,& Vivanco, J. F.. (2020). Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics. in Scientific Reports
Nature Research, Berlin., 10(1), 16493.
https://doi.org/10.1038/s41598-020-73428-y
conv_4875
Benjumeda-Wijnhoven IM, Vallejos R, Santibanez J, Millán CS, Vivanco JF. Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics. in Scientific Reports. 2020;10(1):16493.
doi:10.1038/s41598-020-73428-y
conv_4875 .
Benjumeda-Wijnhoven, Isabel M., Vallejos, Raul, Santibanez, Juan, Millán, Carola S., Vivanco, Juan F., "Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics" in Scientific Reports, 10, no. 1 (2020):16493,
https://doi.org/10.1038/s41598-020-73428-y .,
conv_4875 .
