Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks

2020
Autori
Raileanu, Cristian
Tauchmann, Oliver
Vasić, Ana

Neumann, Ulrike
Tews, Birke Andrea
Silaghi, Cornelia

Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
West Nile virus (WNV) is a mosquito-borne agent that has also been isolated from several tick species. Vector competence of Ixodes ricinus, one of the most common tick species in Europe, has been poorly investigated for WNV to date. As such, to evaluate the vector competence, laboratory reared Ixodes ricinus nymphs were in vitro fed with WNV lineage 1 infectious blood, allowed to molt, and the resulting females artificially fed to study the virus transmission. Furthermore, we studied the kinetics of WNV replication in ticks after infecting nymphs using an automatic injector. Active replication of WNV was detected in injected nymphs from day 7 post-infection until 28 dpi. In the nymphs infected by artificial feeding, the transstadial transmission of WNV was confirmed molecularly in 46.7% of males, while virus transmission during in vitro feeding of I. ricinus females originating from infected nymphs was not registered. The long persistence of WNV in I. ricinus ticks did not correlate wi...th the transmission of the virus and it is unlikely that I. ricinus represents a competent vector. However, there is a potential reservoir role that this tick species can play, with hosts potentially acquiring the viral agent after ingesting the infected ticks.
Ključne reči:
West Nile virus / Ixodes ricinus / transstadial persistence / arbovirusIzvor:
Pathogens, 2020, 9, 10, 780-Izdavač:
- MDPI, Basel
DOI: 10.3390/pathogens9100780
ISSN: 2076-0817
PubMed: 32987685
WoS: 000585162600001
Scopus: 2-s2.0-85091659441
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Raileanu, Cristian AU - Tauchmann, Oliver AU - Vasić, Ana AU - Neumann, Ulrike AU - Tews, Birke Andrea AU - Silaghi, Cornelia PY - 2020 UR - http://rimi.imi.bg.ac.rs/handle/123456789/982 AB - West Nile virus (WNV) is a mosquito-borne agent that has also been isolated from several tick species. Vector competence of Ixodes ricinus, one of the most common tick species in Europe, has been poorly investigated for WNV to date. As such, to evaluate the vector competence, laboratory reared Ixodes ricinus nymphs were in vitro fed with WNV lineage 1 infectious blood, allowed to molt, and the resulting females artificially fed to study the virus transmission. Furthermore, we studied the kinetics of WNV replication in ticks after infecting nymphs using an automatic injector. Active replication of WNV was detected in injected nymphs from day 7 post-infection until 28 dpi. In the nymphs infected by artificial feeding, the transstadial transmission of WNV was confirmed molecularly in 46.7% of males, while virus transmission during in vitro feeding of I. ricinus females originating from infected nymphs was not registered. The long persistence of WNV in I. ricinus ticks did not correlate with the transmission of the virus and it is unlikely that I. ricinus represents a competent vector. However, there is a potential reservoir role that this tick species can play, with hosts potentially acquiring the viral agent after ingesting the infected ticks. PB - MDPI, Basel T2 - Pathogens T1 - Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks IS - 10 SP - 780 VL - 9 DO - 10.3390/pathogens9100780 ER -
@article{ author = "Raileanu, Cristian and Tauchmann, Oliver and Vasić, Ana and Neumann, Ulrike and Tews, Birke Andrea and Silaghi, Cornelia", year = "2020", abstract = "West Nile virus (WNV) is a mosquito-borne agent that has also been isolated from several tick species. Vector competence of Ixodes ricinus, one of the most common tick species in Europe, has been poorly investigated for WNV to date. As such, to evaluate the vector competence, laboratory reared Ixodes ricinus nymphs were in vitro fed with WNV lineage 1 infectious blood, allowed to molt, and the resulting females artificially fed to study the virus transmission. Furthermore, we studied the kinetics of WNV replication in ticks after infecting nymphs using an automatic injector. Active replication of WNV was detected in injected nymphs from day 7 post-infection until 28 dpi. In the nymphs infected by artificial feeding, the transstadial transmission of WNV was confirmed molecularly in 46.7% of males, while virus transmission during in vitro feeding of I. ricinus females originating from infected nymphs was not registered. The long persistence of WNV in I. ricinus ticks did not correlate with the transmission of the virus and it is unlikely that I. ricinus represents a competent vector. However, there is a potential reservoir role that this tick species can play, with hosts potentially acquiring the viral agent after ingesting the infected ticks.", publisher = "MDPI, Basel", journal = "Pathogens", title = "Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks", number = "10", pages = "780", volume = "9", doi = "10.3390/pathogens9100780" }
Raileanu, C., Tauchmann, O., Vasić, A., Neumann, U., Tews, B. A.,& Silaghi, C.. (2020). Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks. in Pathogens MDPI, Basel., 9(10), 780. https://doi.org/10.3390/pathogens9100780
Raileanu C, Tauchmann O, Vasić A, Neumann U, Tews BA, Silaghi C. Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks. in Pathogens. 2020;9(10):780. doi:10.3390/pathogens9100780 .
Raileanu, Cristian, Tauchmann, Oliver, Vasić, Ana, Neumann, Ulrike, Tews, Birke Andrea, Silaghi, Cornelia, "Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks" in Pathogens, 9, no. 10 (2020):780, https://doi.org/10.3390/pathogens9100780 . .