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Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks

Thumbnail
2020
979.pdf (1.258Mb)
Autori
Raileanu, Cristian
Tauchmann, Oliver
Vasić, Ana
Neumann, Ulrike
Tews, Birke Andrea
Silaghi, Cornelia
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
West Nile virus (WNV) is a mosquito-borne agent that has also been isolated from several tick species. Vector competence of Ixodes ricinus, one of the most common tick species in Europe, has been poorly investigated for WNV to date. As such, to evaluate the vector competence, laboratory reared Ixodes ricinus nymphs were in vitro fed with WNV lineage 1 infectious blood, allowed to molt, and the resulting females artificially fed to study the virus transmission. Furthermore, we studied the kinetics of WNV replication in ticks after infecting nymphs using an automatic injector. Active replication of WNV was detected in injected nymphs from day 7 post-infection until 28 dpi. In the nymphs infected by artificial feeding, the transstadial transmission of WNV was confirmed molecularly in 46.7% of males, while virus transmission during in vitro feeding of I. ricinus females originating from infected nymphs was not registered. The long persistence of WNV in I. ricinus ticks did not correlate wi...th the transmission of the virus and it is unlikely that I. ricinus represents a competent vector. However, there is a potential reservoir role that this tick species can play, with hosts potentially acquiring the viral agent after ingesting the infected ticks.

Ključne reči:
West Nile virus / Ixodes ricinus / transstadial persistence / arbovirus
Izvor:
Pathogens, 2020, 9, 10, 780-
Izdavač:
  • MDPI, Basel

DOI: 10.3390/pathogens9100780

ISSN: 2076-0817

PubMed: 32987685

WoS: 000585162600001

Scopus: 2-s2.0-85091659441
[ Google Scholar ]
2
URI
http://rimi.imi.bg.ac.rs/handle/123456789/982
Kolekcije
  • Radovi istraživača / Researchers' publications
Institucija/grupa
Institut za medicinska istraživanja
TY  - JOUR
AU  - Raileanu, Cristian
AU  - Tauchmann, Oliver
AU  - Vasić, Ana
AU  - Neumann, Ulrike
AU  - Tews, Birke Andrea
AU  - Silaghi, Cornelia
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/982
AB  - West Nile virus (WNV) is a mosquito-borne agent that has also been isolated from several tick species. Vector competence of Ixodes ricinus, one of the most common tick species in Europe, has been poorly investigated for WNV to date. As such, to evaluate the vector competence, laboratory reared Ixodes ricinus nymphs were in vitro fed with WNV lineage 1 infectious blood, allowed to molt, and the resulting females artificially fed to study the virus transmission. Furthermore, we studied the kinetics of WNV replication in ticks after infecting nymphs using an automatic injector. Active replication of WNV was detected in injected nymphs from day 7 post-infection until 28 dpi. In the nymphs infected by artificial feeding, the transstadial transmission of WNV was confirmed molecularly in 46.7% of males, while virus transmission during in vitro feeding of I. ricinus females originating from infected nymphs was not registered. The long persistence of WNV in I. ricinus ticks did not correlate with the transmission of the virus and it is unlikely that I. ricinus represents a competent vector. However, there is a potential reservoir role that this tick species can play, with hosts potentially acquiring the viral agent after ingesting the infected ticks.
PB  - MDPI, Basel
T2  - Pathogens
T1  - Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks
IS  - 10
SP  - 780
VL  - 9
DO  - 10.3390/pathogens9100780
ER  - 
@article{
author = "Raileanu, Cristian and Tauchmann, Oliver and Vasić, Ana and Neumann, Ulrike and Tews, Birke Andrea and Silaghi, Cornelia",
year = "2020",
abstract = "West Nile virus (WNV) is a mosquito-borne agent that has also been isolated from several tick species. Vector competence of Ixodes ricinus, one of the most common tick species in Europe, has been poorly investigated for WNV to date. As such, to evaluate the vector competence, laboratory reared Ixodes ricinus nymphs were in vitro fed with WNV lineage 1 infectious blood, allowed to molt, and the resulting females artificially fed to study the virus transmission. Furthermore, we studied the kinetics of WNV replication in ticks after infecting nymphs using an automatic injector. Active replication of WNV was detected in injected nymphs from day 7 post-infection until 28 dpi. In the nymphs infected by artificial feeding, the transstadial transmission of WNV was confirmed molecularly in 46.7% of males, while virus transmission during in vitro feeding of I. ricinus females originating from infected nymphs was not registered. The long persistence of WNV in I. ricinus ticks did not correlate with the transmission of the virus and it is unlikely that I. ricinus represents a competent vector. However, there is a potential reservoir role that this tick species can play, with hosts potentially acquiring the viral agent after ingesting the infected ticks.",
publisher = "MDPI, Basel",
journal = "Pathogens",
title = "Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks",
number = "10",
pages = "780",
volume = "9",
doi = "10.3390/pathogens9100780"
}
Raileanu, C., Tauchmann, O., Vasić, A., Neumann, U., Tews, B. A.,& Silaghi, C.. (2020). Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks. in Pathogens
MDPI, Basel., 9(10), 780.
https://doi.org/10.3390/pathogens9100780
Raileanu C, Tauchmann O, Vasić A, Neumann U, Tews BA, Silaghi C. Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks. in Pathogens. 2020;9(10):780.
doi:10.3390/pathogens9100780 .
Raileanu, Cristian, Tauchmann, Oliver, Vasić, Ana, Neumann, Ulrike, Tews, Birke Andrea, Silaghi, Cornelia, "Transstadial Transmission and Replication Kinetics of West Nile Virus Lineage 1 in Laboratory Reared Ixodes ricinus Ticks" in Pathogens, 9, no. 10 (2020):780,
https://doi.org/10.3390/pathogens9100780 . .

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