Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
Authorized Users Only
2019
Authors
Lazarević, J. J.Ralević, U.
Kukolj, Tamara

Bugarski, Diana

Lazarević, N.

Bugarski, Branko

Popović, Z., V
Article (Published version)

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Show full item recordAbstract
In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different... chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
Keywords:
Raman spectroscopy / Stem cells / Chemical fixationSource:
Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 2019, 216, 173-178Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
- Nanostructured multifunctional materials and nanocomposites (RS-45018)
- Regenerative and modulatory potential of adult stem cells (RS-175062)
- Physics of Ordered Nanostructures and New Materials in Photonics (RS-171005)
DOI: 10.1016/j.saa.2019.03.012
ISSN: 1386-1425
PubMed: 30897378
WoS: 000471317300021
Scopus: 2-s2.0-85062988764
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Institution/Community
Institut za medicinska istraživanjaTY - JOUR AU - Lazarević, J. J. AU - Ralević, U. AU - Kukolj, Tamara AU - Bugarski, Diana AU - Lazarević, N. AU - Bugarski, Branko AU - Popović, Z., V PY - 2019 UR - http://rimi.imi.bg.ac.rs/handle/123456789/966 AB - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy T1 - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy EP - 178 SP - 173 VL - 216 DO - 10.1016/j.saa.2019.03.012 ER -
@article{ author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V", year = "2019", abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy", title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy", pages = "178-173", volume = "216", doi = "10.1016/j.saa.2019.03.012" }
Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178. https://doi.org/10.1016/j.saa.2019.03.012
Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;216:173-178. doi:10.1016/j.saa.2019.03.012 .
Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 216 (2019):173-178, https://doi.org/10.1016/j.saa.2019.03.012 . .