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Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy

Authorized Users Only
2019
Authors
Lazarević, J. J.
Ralević, U.
Kukolj, Tamara
Bugarski, Diana
Lazarević, N.
Bugarski, Branko
Popović, Z., V
Article (Published version)
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Abstract
In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different... chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.

Keywords:
Raman spectroscopy / Stem cells / Chemical fixation
Source:
Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 2019, 216, 173-178
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
  • Nanostructured multifunctional materials and nanocomposites (RS-45018)
  • Regenerative and modulatory potential of adult stem cells (RS-175062)
  • Physics of Ordered Nanostructures and New Materials in Photonics (RS-171005)

DOI: 10.1016/j.saa.2019.03.012

ISSN: 1386-1425

PubMed: 30897378

WoS: 000471317300021

Scopus: 2-s2.0-85062988764
[ Google Scholar ]
2
1
URI
http://rimi.imi.bg.ac.rs/handle/123456789/966
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Lazarević, J. J.
AU  - Ralević, U.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/966
AB  - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1  - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
EP  - 178
SP  - 173
VL  - 216
DO  - 10.1016/j.saa.2019.03.012
UR  - conv_4547
ER  - 
@article{
author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy",
pages = "178-173",
volume = "216",
doi = "10.1016/j.saa.2019.03.012",
url = "conv_4547"
}
Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178.
https://doi.org/10.1016/j.saa.2019.03.012
conv_4547
Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;216:173-178.
doi:10.1016/j.saa.2019.03.012
conv_4547 .
Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 216 (2019):173-178,
https://doi.org/10.1016/j.saa.2019.03.012 .,
conv_4547 .

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