Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy
Samo za registrovane korisnike
2019
Autori
Lazarević, J. J.Kukolj, Tamara
Bugarski, Diana
Lazarević, N.
Bugarski, Branko
Popović, Z., V
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which... can be valuable for medical and clinical application.
Ključne reči:
Raman spectroscopy / Stem cells / DifferentiationIzvor:
Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 2019, 213, 384-390Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Nanostrukturni multifunkcionalni materijali i nanokompoziti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45018)
- Regenerativni i modulatorni potencijal adultnih matičnih ćelija (RS-MESTD-Basic Research (BR or ON)-175062)
DOI: 10.1016/j.saa.2019.01.069
ISSN: 1386-1425
PubMed: 30726762
WoS: 000471314400051
Scopus: 2-s2.0-85061064972
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Lazarević, J. J. AU - Kukolj, Tamara AU - Bugarski, Diana AU - Lazarević, N. AU - Bugarski, Branko AU - Popović, Z., V PY - 2019 UR - http://rimi.imi.bg.ac.rs/handle/123456789/960 AB - We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy T1 - Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy EP - 390 SP - 384 VL - 213 DO - 10.1016/j.saa.2019.01.069 ER -
@article{ author = "Lazarević, J. J. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V", year = "2019", abstract = "We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy", title = "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy", pages = "390-384", volume = "213", doi = "10.1016/j.saa.2019.01.069" }
Lazarević, J. J., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy Pergamon-Elsevier Science Ltd, Oxford., 213, 384-390. https://doi.org/10.1016/j.saa.2019.01.069
Lazarević JJ, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;213:384-390. doi:10.1016/j.saa.2019.01.069 .
Lazarević, J. J., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 213 (2019):384-390, https://doi.org/10.1016/j.saa.2019.01.069 . .