We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which... can be valuable for medical and clinical application.
Keywords:
Raman spectroscopy / Stem cells / Differentiation
Source:
Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 2019, 213, 384-390
TY - JOUR
AU - Lazarević, J. J.
AU - Kukolj, Tamara
AU - Bugarski, Diana
AU - Lazarević, N.
AU - Bugarski, Branko
AU - Popović, Z., V
PY - 2019
UR - http://rimi.imi.bg.ac.rs/handle/123456789/960
AB - We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1 - Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy
EP - 390
SP - 384
VL - 213
DO - 10.1016/j.saa.2019.01.069
UR - conv_4546
ER -
@article{
author = "Lazarević, J. J. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy",
pages = "390-384",
volume = "213",
doi = "10.1016/j.saa.2019.01.069",
url = "conv_4546"
}
Lazarević, J. J., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 213, 384-390.
https://doi.org/10.1016/j.saa.2019.01.069
conv_4546
Lazarević JJ, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;213:384-390.
doi:10.1016/j.saa.2019.01.069
conv_4546 .
Lazarević, J. J., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 213 (2019):384-390,
https://doi.org/10.1016/j.saa.2019.01.069 .,
conv_4546 .
