Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bioassays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6,.lt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III i...s more frequently found in samples from Southern Europe than in those from other parts of the continent.
TY - JOUR
AU - Kuruca, Ljiljana
AU - Uzelac, Aleksandra
AU - Klun, Ivana
AU - Lalošević, Vesna
AU - Đurković-Đaković, Olgica
PY - 2019
UR - http://rimi.imi.bg.ac.rs/handle/123456789/954
AB - Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bioassays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6,.lt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.
PB - Akademiai Kiado Zrt, Budapest
T2 - Acta Veterinaria Hungarica
T1 - Toxoplasma gondii genotypes circulating in domestic pigs in Serbia
EP - 211
IS - 2
SP - 204
VL - 67
DO - 10.1556/004.2019.022
UR - conv_4579
ER -
@article{
author = "Kuruca, Ljiljana and Uzelac, Aleksandra and Klun, Ivana and Lalošević, Vesna and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bioassays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6,.lt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Toxoplasma gondii genotypes circulating in domestic pigs in Serbia",
pages = "211-204",
number = "2",
volume = "67",
doi = "10.1556/004.2019.022",
url = "conv_4579"
}
Kuruca, L., Uzelac, A., Klun, I., Lalošević, V.,& Đurković-Đaković, O.. (2019). Toxoplasma gondii genotypes circulating in domestic pigs in Serbia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 67(2), 204-211.
https://doi.org/10.1556/004.2019.022
conv_4579
Kuruca L, Uzelac A, Klun I, Lalošević V, Đurković-Đaković O. Toxoplasma gondii genotypes circulating in domestic pigs in Serbia. in Acta Veterinaria Hungarica. 2019;67(2):204-211.
doi:10.1556/004.2019.022
conv_4579 .
