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In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice

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2004
92.pdf (139.6Kb)
Authors
Jovčić, Gordana
Bugarski, Diana
Petakov, Marijana
Krstić, A
Vlaški, Marija
Stojanović, N.
Milenković, P.
Article (Published version)
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Abstract
In order to gain more insight into mechanisms operating on the haematopoietic activity of the T-cell-derived cytokine, interleukin-17 (IL-17) and target cells that first respond to its action in vivo, the influence of a single intravenous injection of recombinant mouse IL-17 on bone marrow progenitors, further morphologically recognizable cells and peripheral blood cells was assessed in normal mice up to 72 h after treatment. Simultaneously, the release of IL-6, IL-10, IGF-I, IFN-gamma and NO by bone marrow cells was determined. Results showed that, in bone marrow, IL-17 did not affect granulocyte-macrophage (CFU-GM) progenitors, but induced a persistant increase in the number of morphologically recognizable proliferative granulocytes (PG) up to 48 h after treatment. The number of immature erythroid (BFU-E) progenitors was increased at 48 h, while the number of mature erythroid (CFU-E) progenitors was decreased up to 48 h. In peripheral blood, white blood cells were increased 6 h after... treatment, mainly because of the increase in the number of lymphocytes. IL-17 also increased IL-6 release and NO production 6 h after administration. Additional in vitro assessment on bone marrow highly enriched Lin(-) progenitor cells, demonstrated a slightly enhancing effect of IL-17 on CFU-GM and no influence on BFU-E, suggesting the importance of bone marrow accessory cells and secondary induced cytokines for IL-17 mediated effects on progenitor cells. Taken together, these results demonstrate that in vivo IL-17 affects both granulocytic and erythroid lineages, with more mature haematopoietic progenitors responding first to its action. The opposite effects exerted on PG and CFU-E found at the same time indicate that IL-17, as a component of a regulatory network, is able to intervene in mechanisms that shift haematopoiesis from the erythroid to the granulocytic lineage.

Source:
Cell Proliferation, 2004, 37, 6, 401-412
Publisher:
  • Wiley, Hoboken

DOI: 10.1111/j.1365-2184.2004.00322.x

ISSN: 0960-7722

PubMed: 15548173

WoS: 000225187800002

Scopus: 2-s2.0-9744283467
[ Google Scholar ]
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26
URI
http://rimi.imi.bg.ac.rs/handle/123456789/95
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Petakov, Marijana
AU  - Krstić, A
AU  - Vlaški, Marija
AU  - Stojanović, N.
AU  - Milenković, P.
PY  - 2004
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/95
AB  - In order to gain more insight into mechanisms operating on the haematopoietic activity of the T-cell-derived cytokine, interleukin-17 (IL-17) and target cells that first respond to its action in vivo, the influence of a single intravenous injection of recombinant mouse IL-17 on bone marrow progenitors, further morphologically recognizable cells and peripheral blood cells was assessed in normal mice up to 72 h after treatment. Simultaneously, the release of IL-6, IL-10, IGF-I, IFN-gamma and NO by bone marrow cells was determined. Results showed that, in bone marrow, IL-17 did not affect granulocyte-macrophage (CFU-GM) progenitors, but induced a persistant increase in the number of morphologically recognizable proliferative granulocytes (PG) up to 48 h after treatment. The number of immature erythroid (BFU-E) progenitors was increased at 48 h, while the number of mature erythroid (CFU-E) progenitors was decreased up to 48 h. In peripheral blood, white blood cells were increased 6 h after treatment, mainly because of the increase in the number of lymphocytes. IL-17 also increased IL-6 release and NO production 6 h after administration. Additional in vitro assessment on bone marrow highly enriched Lin(-) progenitor cells, demonstrated a slightly enhancing effect of IL-17 on CFU-GM and no influence on BFU-E, suggesting the importance of bone marrow accessory cells and secondary induced cytokines for IL-17 mediated effects on progenitor cells. Taken together, these results demonstrate that in vivo IL-17 affects both granulocytic and erythroid lineages, with more mature haematopoietic progenitors responding first to its action. The opposite effects exerted on PG and CFU-E found at the same time indicate that IL-17, as a component of a regulatory network, is able to intervene in mechanisms that shift haematopoiesis from the erythroid to the granulocytic lineage.
PB  - Wiley, Hoboken
T2  - Cell Proliferation
T1  - In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice
EP  - 412
IS  - 6
SP  - 401
VL  - 37
DO  - 10.1111/j.1365-2184.2004.00322.x
UR  - conv_1595
ER  - 
@article{
author = "Jovčić, Gordana and Bugarski, Diana and Petakov, Marijana and Krstić, A and Vlaški, Marija and Stojanović, N. and Milenković, P.",
year = "2004",
abstract = "In order to gain more insight into mechanisms operating on the haematopoietic activity of the T-cell-derived cytokine, interleukin-17 (IL-17) and target cells that first respond to its action in vivo, the influence of a single intravenous injection of recombinant mouse IL-17 on bone marrow progenitors, further morphologically recognizable cells and peripheral blood cells was assessed in normal mice up to 72 h after treatment. Simultaneously, the release of IL-6, IL-10, IGF-I, IFN-gamma and NO by bone marrow cells was determined. Results showed that, in bone marrow, IL-17 did not affect granulocyte-macrophage (CFU-GM) progenitors, but induced a persistant increase in the number of morphologically recognizable proliferative granulocytes (PG) up to 48 h after treatment. The number of immature erythroid (BFU-E) progenitors was increased at 48 h, while the number of mature erythroid (CFU-E) progenitors was decreased up to 48 h. In peripheral blood, white blood cells were increased 6 h after treatment, mainly because of the increase in the number of lymphocytes. IL-17 also increased IL-6 release and NO production 6 h after administration. Additional in vitro assessment on bone marrow highly enriched Lin(-) progenitor cells, demonstrated a slightly enhancing effect of IL-17 on CFU-GM and no influence on BFU-E, suggesting the importance of bone marrow accessory cells and secondary induced cytokines for IL-17 mediated effects on progenitor cells. Taken together, these results demonstrate that in vivo IL-17 affects both granulocytic and erythroid lineages, with more mature haematopoietic progenitors responding first to its action. The opposite effects exerted on PG and CFU-E found at the same time indicate that IL-17, as a component of a regulatory network, is able to intervene in mechanisms that shift haematopoiesis from the erythroid to the granulocytic lineage.",
publisher = "Wiley, Hoboken",
journal = "Cell Proliferation",
title = "In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice",
pages = "412-401",
number = "6",
volume = "37",
doi = "10.1111/j.1365-2184.2004.00322.x",
url = "conv_1595"
}
Jovčić, G., Bugarski, D., Petakov, M., Krstić, A., Vlaški, M., Stojanović, N.,& Milenković, P.. (2004). In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice. in Cell Proliferation
Wiley, Hoboken., 37(6), 401-412.
https://doi.org/10.1111/j.1365-2184.2004.00322.x
conv_1595
Jovčić G, Bugarski D, Petakov M, Krstić A, Vlaški M, Stojanović N, Milenković P. In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice. in Cell Proliferation. 2004;37(6):401-412.
doi:10.1111/j.1365-2184.2004.00322.x
conv_1595 .
Jovčić, Gordana, Bugarski, Diana, Petakov, Marijana, Krstić, A, Vlaški, Marija, Stojanović, N., Milenković, P., "In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice" in Cell Proliferation, 37, no. 6 (2004):401-412,
https://doi.org/10.1111/j.1365-2184.2004.00322.x .,
conv_1595 .

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