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How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions

Authorized Users Only
2019
Authors
Bruger, Annika M.
Dorhoi, Anca
Esendagli, Gunes
Barczyk-Kahlert, Katarzyna
van der Bruggen, Pierre
Lipoldova, Marie
Perecko, Tomas
Santibanez, Juan
Saraiva, Margarida
Van Ginderachter, Jo A.
Brandau, Sven
Article (Published version)
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Abstract
Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assa...ys. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.

Keywords:
Myeloid-derived suppressor cells / T cells / Immunosuppression / Arginase / Proliferation / Mye-EUNITER
Source:
Cancer Immunology Immunotherapy, 2019, 68, 4, 631-644
Publisher:
  • Springer, New York
Funding / projects:
  • COST action
  • COST action [BM1404 - Mye-EUNITER]

DOI: 10.1007/s00262-018-2170-8

ISSN: 0340-7004

PubMed: 29785656

WoS: 000463762200011

Scopus: 2-s2.0-85047222918
[ Google Scholar ]
73
55
URI
http://rimi.imi.bg.ac.rs/handle/123456789/947
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Bruger, Annika M.
AU  - Dorhoi, Anca
AU  - Esendagli, Gunes
AU  - Barczyk-Kahlert, Katarzyna
AU  - van der Bruggen, Pierre
AU  - Lipoldova, Marie
AU  - Perecko, Tomas
AU  - Santibanez, Juan
AU  - Saraiva, Margarida
AU  - Van Ginderachter, Jo A.
AU  - Brandau, Sven
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/947
AB  - Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assays. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.
PB  - Springer, New York
T2  - Cancer Immunology Immunotherapy
T1  - How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions
EP  - 644
IS  - 4
SP  - 631
VL  - 68
DO  - 10.1007/s00262-018-2170-8
ER  - 
@article{
author = "Bruger, Annika M. and Dorhoi, Anca and Esendagli, Gunes and Barczyk-Kahlert, Katarzyna and van der Bruggen, Pierre and Lipoldova, Marie and Perecko, Tomas and Santibanez, Juan and Saraiva, Margarida and Van Ginderachter, Jo A. and Brandau, Sven",
year = "2019",
abstract = "Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assays. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.",
publisher = "Springer, New York",
journal = "Cancer Immunology Immunotherapy",
title = "How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions",
pages = "644-631",
number = "4",
volume = "68",
doi = "10.1007/s00262-018-2170-8"
}
Bruger, A. M., Dorhoi, A., Esendagli, G., Barczyk-Kahlert, K., van der Bruggen, P., Lipoldova, M., Perecko, T., Santibanez, J., Saraiva, M., Van Ginderachter, J. A.,& Brandau, S.. (2019). How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions. in Cancer Immunology Immunotherapy
Springer, New York., 68(4), 631-644.
https://doi.org/10.1007/s00262-018-2170-8
conv_4510
Bruger AM, Dorhoi A, Esendagli G, Barczyk-Kahlert K, van der Bruggen P, Lipoldova M, Perecko T, Santibanez J, Saraiva M, Van Ginderachter JA, Brandau S. How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions. in Cancer Immunology Immunotherapy. 2019;68(4):631-644.
doi:10.1007/s00262-018-2170-8
conv_4510 .
Bruger, Annika M., Dorhoi, Anca, Esendagli, Gunes, Barczyk-Kahlert, Katarzyna, van der Bruggen, Pierre, Lipoldova, Marie, Perecko, Tomas, Santibanez, Juan, Saraiva, Margarida, Van Ginderachter, Jo A., Brandau, Sven, "How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions" in Cancer Immunology Immunotherapy, 68, no. 4 (2019):631-644,
https://doi.org/10.1007/s00262-018-2170-8 .,
conv_4510 .

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