How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions
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2019
Authors
Bruger, Annika M.Dorhoi, Anca
Esendagli, Gunes

Barczyk-Kahlert, Katarzyna
van der Bruggen, Pierre
Lipoldova, Marie

Perecko, Tomas
Santibanez, Juan

Saraiva, Margarida

Van Ginderachter, Jo A.

Brandau, Sven
Article (Published version)

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Show full item recordAbstract
Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assa...ys. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.
Keywords:
Myeloid-derived suppressor cells / T cells / Immunosuppression / Arginase / Proliferation / Mye-EUNITERSource:
Cancer Immunology Immunotherapy, 2019, 68, 4, 631-644Publisher:
- Springer, New York
Funding / projects:
- COST action
- COST action [BM1404 - Mye-EUNITER]
DOI: 10.1007/s00262-018-2170-8
ISSN: 0340-7004
PubMed: 29785656
WoS: 000463762200011
Scopus: 2-s2.0-85047222918
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Institut za medicinska istraživanjaTY - JOUR AU - Bruger, Annika M. AU - Dorhoi, Anca AU - Esendagli, Gunes AU - Barczyk-Kahlert, Katarzyna AU - van der Bruggen, Pierre AU - Lipoldova, Marie AU - Perecko, Tomas AU - Santibanez, Juan AU - Saraiva, Margarida AU - Van Ginderachter, Jo A. AU - Brandau, Sven PY - 2019 UR - http://rimi.imi.bg.ac.rs/handle/123456789/947 AB - Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assays. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC. PB - Springer, New York T2 - Cancer Immunology Immunotherapy T1 - How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions EP - 644 IS - 4 SP - 631 VL - 68 DO - 10.1007/s00262-018-2170-8 ER -
@article{ author = "Bruger, Annika M. and Dorhoi, Anca and Esendagli, Gunes and Barczyk-Kahlert, Katarzyna and van der Bruggen, Pierre and Lipoldova, Marie and Perecko, Tomas and Santibanez, Juan and Saraiva, Margarida and Van Ginderachter, Jo A. and Brandau, Sven", year = "2019", abstract = "Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of mononuclear and polymorphonuclear myeloid cells, which are present at very low numbers in healthy subjects, but can expand substantially under disease conditions. Depending on disease type and stage, MDSC comprise varying amounts of immature and mature differentiation stages of myeloid cells. Validated unique phenotypic markers for MDSC are still lacking. Therefore, the functional analysis of these cells is of central importance for their identification and characterization. Various disease-promoting and immunosuppressive functions of MDSC are reported in the literature. Among those, the capacity to modulate the activity of T cells is by far the most often used and best-established read-out system. In this review, we critically evaluate the assays available for the functional analysis of human and murine MDSC under in vitro and in vivo conditions. We also discuss critical issues and controls associated with those assays. We aim at providing suggestions and recommendations useful for the contemporary biological characterization of MDSC.", publisher = "Springer, New York", journal = "Cancer Immunology Immunotherapy", title = "How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions", pages = "644-631", number = "4", volume = "68", doi = "10.1007/s00262-018-2170-8" }
Bruger, A. M., Dorhoi, A., Esendagli, G., Barczyk-Kahlert, K., van der Bruggen, P., Lipoldova, M., Perecko, T., Santibanez, J., Saraiva, M., Van Ginderachter, J. A.,& Brandau, S.. (2019). How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions. in Cancer Immunology Immunotherapy Springer, New York., 68(4), 631-644. https://doi.org/10.1007/s00262-018-2170-8
Bruger AM, Dorhoi A, Esendagli G, Barczyk-Kahlert K, van der Bruggen P, Lipoldova M, Perecko T, Santibanez J, Saraiva M, Van Ginderachter JA, Brandau S. How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions. in Cancer Immunology Immunotherapy. 2019;68(4):631-644. doi:10.1007/s00262-018-2170-8 .
Bruger, Annika M., Dorhoi, Anca, Esendagli, Gunes, Barczyk-Kahlert, Katarzyna, van der Bruggen, Pierre, Lipoldova, Marie, Perecko, Tomas, Santibanez, Juan, Saraiva, Margarida, Van Ginderachter, Jo A., Brandau, Sven, "How to measure the immunosuppressive activity of MDSC: assays, problems and potential solutions" in Cancer Immunology Immunotherapy, 68, no. 4 (2019):631-644, https://doi.org/10.1007/s00262-018-2170-8 . .