Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro
Abstract
Mesenchymal stem/stromal cells (MSCs) are an essential element of most modem tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease - including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approache...s to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve bio-chemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.
Keywords:
Mesenchymal stem/stromal cells / skeletal progenitor cells / niche / in vitro models / bone / tissue engineeringSource:
European Cells & Materials, 2019, 37, 88-112Publisher:
- Ao Research Institute Davos-Ari, Davos
Funding / projects:
- Interdisciplinary Center for Clinical Research (IZKF) Wuerzburg, Germany [D-361]
DOI: 10.22203/eCM.v037a07
ISSN: 1473-2262
PubMed: 30742298
WoS: 000463430000007
Scopus: 2-s2.0-85061268226
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Institution/Community
Institut za medicinska istraživanjaTY - JOUR AU - Pereira, A. R. AU - Trivanović, Drenka AU - Herrmann, Marietta PY - 2019 UR - http://rimi.imi.bg.ac.rs/handle/123456789/939 AB - Mesenchymal stem/stromal cells (MSCs) are an essential element of most modem tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease - including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve bio-chemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs. PB - Ao Research Institute Davos-Ari, Davos T2 - European Cells & Materials T1 - Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro EP - 112 SP - 88 VL - 37 DO - 10.22203/eCM.v037a07 ER -
@article{ author = "Pereira, A. R. and Trivanović, Drenka and Herrmann, Marietta", year = "2019", abstract = "Mesenchymal stem/stromal cells (MSCs) are an essential element of most modem tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease - including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve bio-chemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.", publisher = "Ao Research Institute Davos-Ari, Davos", journal = "European Cells & Materials", title = "Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro", pages = "112-88", volume = "37", doi = "10.22203/eCM.v037a07" }
Pereira, A. R., Trivanović, D.,& Herrmann, M.. (2019). Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro. in European Cells & Materials Ao Research Institute Davos-Ari, Davos., 37, 88-112. https://doi.org/10.22203/eCM.v037a07 conv_4505
Pereira AR, Trivanović D, Herrmann M. Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro. in European Cells & Materials. 2019;37:88-112. doi:10.22203/eCM.v037a07 conv_4505 .
Pereira, A. R., Trivanović, Drenka, Herrmann, Marietta, "Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro" in European Cells & Materials, 37 (2019):88-112, https://doi.org/10.22203/eCM.v037a07 ., conv_4505 .