Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia
Само за регистроване кориснике
2019
Аутори
Ćakić, SanjaVeinović, Gorana
Cerar, T.
Mihaljica, Darko
Sukara, Ratko
Ruzic-Sabljić, E.
Tomanović, Snežana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype ...the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.
Кључне речи:
Borrelia burgdorferi s / l / cultivation / characterization / Ixodes ricinus / pathogen / quantitative PCR / vectorИзвор:
Medical & Veterinary Entomology, 2019, 33, 4, 512-520Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Ензоотски трансмисиони циклуси патогених микроорганизама које преносе крпељи (RS-MESTD-Basic Research (BR or ON)-173006)
DOI: 10.1111/mve.12392
ISSN: 0269-283X
PubMed: 31173386
WoS: 000493660400008
Scopus: 2-s2.0-85067392584
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Ćakić, Sanja AU - Veinović, Gorana AU - Cerar, T. AU - Mihaljica, Darko AU - Sukara, Ratko AU - Ruzic-Sabljić, E. AU - Tomanović, Snežana PY - 2019 UR - http://rimi.imi.bg.ac.rs/handle/123456789/934 AB - Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia. PB - Wiley, Hoboken T2 - Medical & Veterinary Entomology T1 - Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia EP - 520 IS - 4 SP - 512 VL - 33 DO - 10.1111/mve.12392 ER -
@article{ author = "Ćakić, Sanja and Veinović, Gorana and Cerar, T. and Mihaljica, Darko and Sukara, Ratko and Ruzic-Sabljić, E. and Tomanović, Snežana", year = "2019", abstract = "Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.", publisher = "Wiley, Hoboken", journal = "Medical & Veterinary Entomology", title = "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia", pages = "520-512", number = "4", volume = "33", doi = "10.1111/mve.12392" }
Ćakić, S., Veinović, G., Cerar, T., Mihaljica, D., Sukara, R., Ruzic-Sabljić, E.,& Tomanović, S.. (2019). Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology Wiley, Hoboken., 33(4), 512-520. https://doi.org/10.1111/mve.12392
Ćakić S, Veinović G, Cerar T, Mihaljica D, Sukara R, Ruzic-Sabljić E, Tomanović S. Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology. 2019;33(4):512-520. doi:10.1111/mve.12392 .
Ćakić, Sanja, Veinović, Gorana, Cerar, T., Mihaljica, Darko, Sukara, Ratko, Ruzic-Sabljić, E., Tomanović, Snežana, "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia" in Medical & Veterinary Entomology, 33, no. 4 (2019):512-520, https://doi.org/10.1111/mve.12392 . .