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Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study

Authorized Users Only
2019
Authors
Živković, Lada
Bajić, Vladan
Bruić, Marija
Borozan, Sunčica
Popić, Kristina
Topalović, Dijana
Santibanez, Juan
Spremo-Potparević, Biljana
Article (Published version)
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Abstract
Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage... after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell's repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.

Keywords:
Immune Assist (IA) / Antigenotoxicity / center dot OH Scavenging properties
Source:
Mutation Research-Genetic Toxicology & Environmental Mutagenesis, 2019, 845
Publisher:
  • Elsevier, Amsterdam
Funding / projects:
  • Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-173034)
  • COST action [15132 - hCOMET]

DOI: 10.1016/j.mrgentox.2019.06.008

ISSN: 1383-5718

PubMed: 31561902

WoS: 000489193400018

Scopus: 2-s2.0-85070315567
[ Google Scholar ]
13
7
URI
http://rimi.imi.bg.ac.rs/handle/123456789/915
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/915
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell's repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology & Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 
@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell's repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology & Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
doi = "10.1016/j.mrgentox.2019.06.008"
}
Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research-Genetic Toxicology & Environmental Mutagenesis
Elsevier, Amsterdam., 845.
https://doi.org/10.1016/j.mrgentox.2019.06.008
conv_4641
Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez J, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research-Genetic Toxicology & Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2019.06.008
conv_4641 .
Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research-Genetic Toxicology & Environmental Mutagenesis, 845 (2019),
https://doi.org/10.1016/j.mrgentox.2019.06.008 .,
conv_4641 .

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