Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates
Само за регистроване кориснике
2018
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background. Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations. Materials and methods. Buffy-coat-derived platelet concentrates were stored under standard conditions for 5 days. The expression of activation antigens CD42b, CD36, CD62p and phosphatidylserine on total platelets and populations of small, medium-sized and large platelets was analysed by flow cytometry on storage days 1, 3 and 5. Results. The activation/lesion on total platelets and each platelet population was detected on storage day 3, by the increased expression of CD36. On the same day, increased expression of CD42b and CD62p was detected, but only on large platelets. Small and medium-sized platelets had increased CD62p expression only on day 5. Externalisation of phosphatidylserine was not detected. Discussi...on. Evaluation of the level of expression of various activation markers on different platelet populations could be an additional valid analysis in cell quality control of platelet concentrates, and in the assessment of novel approaches to platelet concentrate manipulation.
Кључне речи:
platelet storage lesion / platelet glycoproteins / flow cytometry / buffy coatИзвор:
Blood Transfusion, 2018, 16, 1, 83-92Издавач:
- Simti Servizi Srl, Milan
Финансирање / пројекти:
- Ministry of Defence of the Republic of Serbia [VMA/06-10B.20]
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-175062)
DOI: 10.2450/2016.0193-16
ISSN: 1723-2007
PubMed: 28166864
WoS: 000425255100012
Scopus: 2-s2.0-85041237577
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Vučetić, Dušan AU - Ilić, Vesna AU - Vojvodić, Danilo AU - Subota, Vesna AU - Todorović, Milena AU - Balint, Bela PY - 2018 UR - http://rimi.imi.bg.ac.rs/handle/123456789/869 AB - Background. Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations. Materials and methods. Buffy-coat-derived platelet concentrates were stored under standard conditions for 5 days. The expression of activation antigens CD42b, CD36, CD62p and phosphatidylserine on total platelets and populations of small, medium-sized and large platelets was analysed by flow cytometry on storage days 1, 3 and 5. Results. The activation/lesion on total platelets and each platelet population was detected on storage day 3, by the increased expression of CD36. On the same day, increased expression of CD42b and CD62p was detected, but only on large platelets. Small and medium-sized platelets had increased CD62p expression only on day 5. Externalisation of phosphatidylserine was not detected. Discussion. Evaluation of the level of expression of various activation markers on different platelet populations could be an additional valid analysis in cell quality control of platelet concentrates, and in the assessment of novel approaches to platelet concentrate manipulation. PB - Simti Servizi Srl, Milan T2 - Blood Transfusion T1 - Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates EP - 92 IS - 1 SP - 83 VL - 16 DO - 10.2450/2016.0193-16 ER -
@article{ author = "Vučetić, Dušan and Ilić, Vesna and Vojvodić, Danilo and Subota, Vesna and Todorović, Milena and Balint, Bela", year = "2018", abstract = "Background. Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations. Materials and methods. Buffy-coat-derived platelet concentrates were stored under standard conditions for 5 days. The expression of activation antigens CD42b, CD36, CD62p and phosphatidylserine on total platelets and populations of small, medium-sized and large platelets was analysed by flow cytometry on storage days 1, 3 and 5. Results. The activation/lesion on total platelets and each platelet population was detected on storage day 3, by the increased expression of CD36. On the same day, increased expression of CD42b and CD62p was detected, but only on large platelets. Small and medium-sized platelets had increased CD62p expression only on day 5. Externalisation of phosphatidylserine was not detected. Discussion. Evaluation of the level of expression of various activation markers on different platelet populations could be an additional valid analysis in cell quality control of platelet concentrates, and in the assessment of novel approaches to platelet concentrate manipulation.", publisher = "Simti Servizi Srl, Milan", journal = "Blood Transfusion", title = "Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates", pages = "92-83", number = "1", volume = "16", doi = "10.2450/2016.0193-16" }
Vučetić, D., Ilić, V., Vojvodić, D., Subota, V., Todorović, M.,& Balint, B.. (2018). Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates. in Blood Transfusion Simti Servizi Srl, Milan., 16(1), 83-92. https://doi.org/10.2450/2016.0193-16
Vučetić D, Ilić V, Vojvodić D, Subota V, Todorović M, Balint B. Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates. in Blood Transfusion. 2018;16(1):83-92. doi:10.2450/2016.0193-16 .
Vučetić, Dušan, Ilić, Vesna, Vojvodić, Danilo, Subota, Vesna, Todorović, Milena, Balint, Bela, "Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates" in Blood Transfusion, 16, no. 1 (2018):83-92, https://doi.org/10.2450/2016.0193-16 . .