RIMI - Repository of the Institute for Medical Research
Institute for Medical Research
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   RIMI
  • Institut za medicinska istraživanja
  • Radovi istraživača / Researchers' publications
  • View Item
  •   RIMI
  • Institut za medicinska istraživanja
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Optimization of extraction and characterization of Urtica dioica L. leaf extract for the evaluation of dose-dependent response in experimental hypertension

Thumbnail
2018
836.pdf (4.903Mb)
Authors
Vajić, Una-Jovana
Metadata
Show full item record
Abstract
This doctoral dissertation was focused on the optimization of the extraction parameters to achieve the maximum yield of phenolic compounds in the Urtica dioica L. leaf extracts. The extract with the highest content of phenolic compounds was tested in the model of experimental hypertension to determine its effects on oxidative and hemodynamic status, lipid profile, and biochemical parameters. It was shown that the most abundant phenolic compounds present in extracts of Urtica dioica L. were chlorogenic acid, 2-O-caffeoyl malic acid, and rutin. It was shown that aqueous-methanol is a better solvent for the extraction of phenolic compounds compared to aqueous-ethanol and water. Also, extracts prepared using ultrasound-assisted extraction technique had higher phenolic yields and antioxidant capacities than extracts obtained by maceration. The maximal content of phenolic compounds in the Urtica dioica L. leaf extracts was achieved by ultrasound-assisted extraction with 54% aqueous-methanol ...and 38 min extraction time. It was shown that the methanol percentage in the extraction solvent significantly affects phenolic yield, unlike the extraction time. Results of in vivo study showed that four-week long treatment with Urtica dioica L. leaf extract decreased arterial pressure and improved systemic oxidative status of spontaneously hypertensive rats, as well as the lipid status by increasing plasma HDL cholesterol and decreasing atherosclerosis-index.

Keywords:
optimization of extraction / stinging nettle / Urtica dioica L. / phenolic compounds / hypertension / oxidative stress / Nema
Source:
Lekovite sirovine, 2018, 38, 81-84
Publisher:
  • Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
Funding / projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)

DOI: 10.5937/leksir1838081V

ISSN: 0455-6224

[ Google Scholar ]
URI
http://rimi.imi.bg.ac.rs/handle/123456789/839
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
Vajić, U.. (2018). Optimization of extraction and characterization of Urtica dioica L. leaf extract for the evaluation of dose-dependent response in experimental hypertension. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(38), 81-84.
https://doi.org/10.5937/leksir1838081V
conv_1162
Vajić U. Optimization of extraction and characterization of Urtica dioica L. leaf extract for the evaluation of dose-dependent response in experimental hypertension. in Lekovite sirovine. 2018;(38):81-84.
doi:10.5937/leksir1838081V
conv_1162 .
Vajić, Una-Jovana, "Optimization of extraction and characterization of Urtica dioica L. leaf extract for the evaluation of dose-dependent response in experimental hypertension" in Lekovite sirovine, no. 38 (2018):81-84,
https://doi.org/10.5937/leksir1838081V .,
conv_1162 .

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB