Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation
Samo za registrovane korisnike
2017
Autori
Mihaljica, DarkoMarković, Dragana
Radulović, Eljko
Mulenga, Albert
Ćakić, Sanja
Sukara, Ratko
Samardzić, Jelena
Tomanović, Snežana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.
Ključne reči:
Tick bite markers / Salivary proteins / Exposure to ticks / Ixodes ricinusIzvor:
Ticks & Tick-Borne Diseases, 2017, 8, 3, 391-395Izdavač:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Finansiranje / projekti:
DOI: 10.1016/j.ttbdis.2017.01.001
ISSN: 1877-959X
PubMed: 28119040
WoS: 000395213600010
Scopus: 2-s2.0-85010006305
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Mihaljica, Darko AU - Marković, Dragana AU - Radulović, Eljko AU - Mulenga, Albert AU - Ćakić, Sanja AU - Sukara, Ratko AU - Samardzić, Jelena AU - Tomanović, Snežana PY - 2017 UR - http://rimi.imi.bg.ac.rs/handle/123456789/816 AB - Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Ticks & Tick-Borne Diseases T1 - Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation EP - 395 IS - 3 SP - 391 VL - 8 DO - 10.1016/j.ttbdis.2017.01.001 ER -
@article{ author = "Mihaljica, Darko and Marković, Dragana and Radulović, Eljko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Samardzić, Jelena and Tomanović, Snežana", year = "2017", abstract = "Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Ticks & Tick-Borne Diseases", title = "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation", pages = "395-391", number = "3", volume = "8", doi = "10.1016/j.ttbdis.2017.01.001" }
Mihaljica, D., Marković, D., Radulović, E., Mulenga, A., Ćakić, S., Sukara, R., Samardzić, J.,& Tomanović, S.. (2017). Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases Elsevier Gmbh, Urban & Fischer Verlag, Jena., 8(3), 391-395. https://doi.org/10.1016/j.ttbdis.2017.01.001
Mihaljica D, Marković D, Radulović E, Mulenga A, Ćakić S, Sukara R, Samardzić J, Tomanović S. Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases. 2017;8(3):391-395. doi:10.1016/j.ttbdis.2017.01.001 .
Mihaljica, Darko, Marković, Dragana, Radulović, Eljko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Samardzić, Jelena, Tomanović, Snežana, "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation" in Ticks & Tick-Borne Diseases, 8, no. 3 (2017):391-395, https://doi.org/10.1016/j.ttbdis.2017.01.001 . .