Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.
TY - JOUR
AU - Mihaljica, Darko
AU - Marković, Dragana
AU - Radulović, Eljko
AU - Mulenga, Albert
AU - Ćakić, Sanja
AU - Sukara, Ratko
AU - Samardzić, Jelena
AU - Tomanović, Snežana
PY - 2017
UR - http://rimi.imi.bg.ac.rs/handle/123456789/816
AB - Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.
PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2 - Ticks & Tick-Borne Diseases
T1 - Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation
EP - 395
IS - 3
SP - 391
VL - 8
DO - 10.1016/j.ttbdis.2017.01.001
UR - conv_3973
ER -
@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Eljko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Samardzić, Jelena and Tomanović, Snežana",
year = "2017",
abstract = "Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks & Tick-Borne Diseases",
title = "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation",
pages = "395-391",
number = "3",
volume = "8",
doi = "10.1016/j.ttbdis.2017.01.001",
url = "conv_3973"
}
Mihaljica, D., Marković, D., Radulović, E., Mulenga, A., Ćakić, S., Sukara, R., Samardzić, J.,& Tomanović, S.. (2017). Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 8(3), 391-395.
https://doi.org/10.1016/j.ttbdis.2017.01.001
conv_3973
Mihaljica D, Marković D, Radulović E, Mulenga A, Ćakić S, Sukara R, Samardzić J, Tomanović S. Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases. 2017;8(3):391-395.
doi:10.1016/j.ttbdis.2017.01.001
conv_3973 .
Mihaljica, Darko, Marković, Dragana, Radulović, Eljko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Samardzić, Jelena, Tomanović, Snežana, "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation" in Ticks & Tick-Borne Diseases, 8, no. 3 (2017):391-395,
https://doi.org/10.1016/j.ttbdis.2017.01.001 .,
conv_3973 .
