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dc.creatorLeković, Danijela
dc.creatorGotić, Mirjana
dc.creatorSkoda, Radek C.
dc.creatorBeleslin-Čokić, Bojana
dc.creatorMilić, Nataša
dc.creatorMitrović-Ajtić, Olivera
dc.creatorNienhold, Ronny
dc.creatorSefer, Dijana
dc.creatorSubotički, Tijana
dc.creatorKovačić, Marijana
dc.creatorMarković, Dragana
dc.creatorDiklić, Miloš
dc.creatorČokić, Vladan
dc.date.accessioned2021-04-20T12:54:04Z
dc.date.available2021-04-20T12:54:04Z
dc.date.issued2017
dc.identifier.issn0939-5555
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/804
dc.description.abstractIncreased angiogenesis in BCR-ABL1 negative myeloproliferative neoplasms (MPNs) has been recognized, but its connection with clinical and molecular markers needs to be defined. The aims of study were to (1) assess bone marrow (BM) angiogenesis measured by microvessel density (MVD) using CD34 and CD105 antibodies; (2) analyze correlation of MVD with plasma angiogenic factors including vascular endothelial growth factor, basic fibroblast growth factor, and interleukin-8; (3) examine the association of MVD with clinicopathological and molecular markers. We examined 90 de novo MPN patients (30 polycythemia vera (PV), primary myelofibrosis (PMF), essential thrombocythemia (ET)) and 10 age-matched controls. MVD was analyzed by immunohistochemistry "hot spot" method, angiogenic factors by immunoassay and JAK2V617F, and CALR mutations by DNA sequencing and allelic PCR. MVD was significantly increased in MPNs compared to controls (PMF gt PV gt ET). Correlation between MVD and plasma angiogenic factors was found in MPNs. MVD was significantly increased in patients with JAK2V617F mutation and correlated with JAK2 mutant allele burden (CD34-MVD: rho = 0.491, p lt 0.001; CD105-MVD: rho = 0.276, p = 0.02) but not with CALR mutation. MVD correlated with leukocyte count, serum lactate dehydrogenase, hepatomegaly, and splenomegaly. BM fibrosis was significantly associated with CD34-MVD, CD105-MVD, interleukin-8, and JAK2 mutant allele burden. JAK2 homozygote status had positive predictive value (100%) for BM fibrosis. Patients with prefibrotic PMF had significantly higher MVD than patients with ET, and we could recommend MVD to be additional histopathological marker to distinguish these two entities. This study also highlights the strong correlation of MVD with plasma angiogenic factors, JAK2 mutant allele burden, and BM fibrosis in MPNs.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175053/RS//
dc.relationSwiss National Science Foundation through Joint research project (SCOPES) [IZ73Z0_152420/1]
dc.rightsrestrictedAccess
dc.sourceAnnals of Hematology
dc.subjectMyeloproliferative neoplasmsen
dc.subjectAngiogenesisen
dc.subjectMicrovessel densityen
dc.subjectJAK2V617Fen
dc.subjectCALRen
dc.titleBone marrow microvessel density and plasma angiogenic factors in myeloproliferative neoplasms: clinicopathological and molecular correlationsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage404
dc.citation.issue3
dc.citation.other96(3): 393-404
dc.citation.rankM22
dc.citation.spage393
dc.citation.volume96
dc.identifier.doi10.1007/s00277-016-2890-9
dc.identifier.pmid27924369
dc.identifier.scopus2-s2.0-85001816366
dc.identifier.wos000394104500008
dc.type.versionpublishedVersion


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