East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin
2017
Аутори
Hornok, SandorSandor, Attila D.
Tomanović, Snežana
Beck, Relja
D'Amico, Gianluca
Kontschan, Jeno
Takacs, Nora
Gorfol, Tamas
Bendjeddou, Mohammed Lamine
Foldvari, Gabor
Farkas, Robert
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Rhipicephalus sanguineus belongs to a complex of hard tick species with high veterinary-medical significance. Recently, new phylogenetic units have been discovered within R. sanguineus, which therefore needs taxonomic revision. The present study was initiated to provide new information on the phylogeography of relevant haplotypes from less studied regions of Europe and Africa. With this aim, molecular-phylogenetic analyses of two mitochondrial markers were performed on 50 ticks collected in Hungary, the Balkans, countries along the Mediterranean Sea, Kenya and Ivory Coast. Results: In the "temperate lineage" of R. sanguineus, based on cytochrome c oxidase subunit 1 (cox1) and 16S rRNA genes, Rhipicephalus sp. I was only found in the eastern part of the Mediterranean Basin (with relatively homogenous haplotypes), whereas Rhipicephalus sp. II occurred in the middle-to-western part of this region (with phylogenetically dichotomous haplotypes). Ticks identified as R. leporis (b...ased on morphology and cox1 gene) were found in Kenya and Ivory Coast. These clustered phylogenetically within R. sanguineus (s.l.) ("tropical lineage"). Conclusions: In the Mediterranean Basin two mitochondrial lineages of R. sanguineus, i. e. Rhipicephalus sp. I and Rhipicephalus sp. II exist, which show different geographical distribution. Therefore, data from this study confirm limited gene flow between Rhipicephalus sp. I and Rhipicephalus sp. II, but more evidence (analyses of nuclear markers, extensive morphological and biological comparison etc.) are necessary to infer if they belong to different species or not. The phylogenetic relationships of eastern and western African ticks, which align with R. leporis, need to be studied further within R. sanguineus (s.l.) ("tropical lineage").
Кључне речи:
Phylogeography / cox1 / 16S rRNA gene / Rhipicephalus sanguineus / Rhipicephalus leporisИзвор:
Parasites & Vectors, 2017, 10Издавач:
- Biomed Central Ltd, London
Финансирање / пројекти:
- OTKA (Hungary)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [115854]
- Hungarian Academy of Sciences
- Hungarian Ministry of Human Resources [11475-4/2016/FEKUT]
- Ензоотски трансмисиони циклуси патогених микроорганизама које преносе крпељи (RS-MESTD-Basic Research (BR or ON)-173006)
- [PN-II-RU-TE-2014-4-1389]
- [GENOTICKTRECK-1957]
DOI: 10.1186/s13071-017-1985-z
ISSN: 1756-3305
PubMed: 28115024
WoS: 000396326200001
Scopus: 2-s2.0-85010901239
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Hornok, Sandor AU - Sandor, Attila D. AU - Tomanović, Snežana AU - Beck, Relja AU - D'Amico, Gianluca AU - Kontschan, Jeno AU - Takacs, Nora AU - Gorfol, Tamas AU - Bendjeddou, Mohammed Lamine AU - Foldvari, Gabor AU - Farkas, Robert PY - 2017 UR - http://rimi.imi.bg.ac.rs/handle/123456789/802 AB - Background: Rhipicephalus sanguineus belongs to a complex of hard tick species with high veterinary-medical significance. Recently, new phylogenetic units have been discovered within R. sanguineus, which therefore needs taxonomic revision. The present study was initiated to provide new information on the phylogeography of relevant haplotypes from less studied regions of Europe and Africa. With this aim, molecular-phylogenetic analyses of two mitochondrial markers were performed on 50 ticks collected in Hungary, the Balkans, countries along the Mediterranean Sea, Kenya and Ivory Coast. Results: In the "temperate lineage" of R. sanguineus, based on cytochrome c oxidase subunit 1 (cox1) and 16S rRNA genes, Rhipicephalus sp. I was only found in the eastern part of the Mediterranean Basin (with relatively homogenous haplotypes), whereas Rhipicephalus sp. II occurred in the middle-to-western part of this region (with phylogenetically dichotomous haplotypes). Ticks identified as R. leporis (based on morphology and cox1 gene) were found in Kenya and Ivory Coast. These clustered phylogenetically within R. sanguineus (s.l.) ("tropical lineage"). Conclusions: In the Mediterranean Basin two mitochondrial lineages of R. sanguineus, i. e. Rhipicephalus sp. I and Rhipicephalus sp. II exist, which show different geographical distribution. Therefore, data from this study confirm limited gene flow between Rhipicephalus sp. I and Rhipicephalus sp. II, but more evidence (analyses of nuclear markers, extensive morphological and biological comparison etc.) are necessary to infer if they belong to different species or not. The phylogenetic relationships of eastern and western African ticks, which align with R. leporis, need to be studied further within R. sanguineus (s.l.) ("tropical lineage"). PB - Biomed Central Ltd, London T2 - Parasites & Vectors T1 - East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin VL - 10 DO - 10.1186/s13071-017-1985-z ER -
@article{ author = "Hornok, Sandor and Sandor, Attila D. and Tomanović, Snežana and Beck, Relja and D'Amico, Gianluca and Kontschan, Jeno and Takacs, Nora and Gorfol, Tamas and Bendjeddou, Mohammed Lamine and Foldvari, Gabor and Farkas, Robert", year = "2017", abstract = "Background: Rhipicephalus sanguineus belongs to a complex of hard tick species with high veterinary-medical significance. Recently, new phylogenetic units have been discovered within R. sanguineus, which therefore needs taxonomic revision. The present study was initiated to provide new information on the phylogeography of relevant haplotypes from less studied regions of Europe and Africa. With this aim, molecular-phylogenetic analyses of two mitochondrial markers were performed on 50 ticks collected in Hungary, the Balkans, countries along the Mediterranean Sea, Kenya and Ivory Coast. Results: In the "temperate lineage" of R. sanguineus, based on cytochrome c oxidase subunit 1 (cox1) and 16S rRNA genes, Rhipicephalus sp. I was only found in the eastern part of the Mediterranean Basin (with relatively homogenous haplotypes), whereas Rhipicephalus sp. II occurred in the middle-to-western part of this region (with phylogenetically dichotomous haplotypes). Ticks identified as R. leporis (based on morphology and cox1 gene) were found in Kenya and Ivory Coast. These clustered phylogenetically within R. sanguineus (s.l.) ("tropical lineage"). Conclusions: In the Mediterranean Basin two mitochondrial lineages of R. sanguineus, i. e. Rhipicephalus sp. I and Rhipicephalus sp. II exist, which show different geographical distribution. Therefore, data from this study confirm limited gene flow between Rhipicephalus sp. I and Rhipicephalus sp. II, but more evidence (analyses of nuclear markers, extensive morphological and biological comparison etc.) are necessary to infer if they belong to different species or not. The phylogenetic relationships of eastern and western African ticks, which align with R. leporis, need to be studied further within R. sanguineus (s.l.) ("tropical lineage").", publisher = "Biomed Central Ltd, London", journal = "Parasites & Vectors", title = "East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin", volume = "10", doi = "10.1186/s13071-017-1985-z" }
Hornok, S., Sandor, A. D., Tomanović, S., Beck, R., D'Amico, G., Kontschan, J., Takacs, N., Gorfol, T., Bendjeddou, M. L., Foldvari, G.,& Farkas, R.. (2017). East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin. in Parasites & Vectors Biomed Central Ltd, London., 10. https://doi.org/10.1186/s13071-017-1985-z
Hornok S, Sandor AD, Tomanović S, Beck R, D'Amico G, Kontschan J, Takacs N, Gorfol T, Bendjeddou ML, Foldvari G, Farkas R. East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin. in Parasites & Vectors. 2017;10. doi:10.1186/s13071-017-1985-z .
Hornok, Sandor, Sandor, Attila D., Tomanović, Snežana, Beck, Relja, D'Amico, Gianluca, Kontschan, Jeno, Takacs, Nora, Gorfol, Tamas, Bendjeddou, Mohammed Lamine, Foldvari, Gabor, Farkas, Robert, "East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin" in Parasites & Vectors, 10 (2017), https://doi.org/10.1186/s13071-017-1985-z . .