RIMI - Repository of the Institute for Medical Research
Institute for Medical Research
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   RIMI
  • Institut za medicinska istraživanja
  • Radovi istraživača / Researchers' publications
  • View Item
  •   RIMI
  • Institut za medicinska istraživanja
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts

Authorized Users Only
2017
Authors
Mihaljica, Darko
Marković, Dragana
Radulović, Željko
Mulenga, Albert
Ćakić, Sanja
Sukara, Ratko
Milanović, Zorana
Tomanović, Snežana
Article (Published version)
Metadata
Show full item record
Abstract
Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatmen...t. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.

Keywords:
Tick bite marker / Saliva protein / Ixodes ricinus / Hunting dogs
Source:
Experimental & Applied Acarology, 2017, 72, 4, 429-437
Publisher:
  • Springer, Dordrecht
Funding / projects:
  • Enzootic transmission cycles of tick-borne pathogen microorganisms (RS-173006)

DOI: 10.1007/s10493-017-0170-6

ISSN: 0168-8162

PubMed: 28840367

WoS: 000409295500008

Scopus: 2-s2.0-85028338427
[ Google Scholar ]
2
2
URI
http://rimi.imi.bg.ac.rs/handle/123456789/762
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Željko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Milanović, Zorana
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/762
AB  - Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts
EP  - 437
IS  - 4
SP  - 429
VL  - 72
DO  - 10.1007/s10493-017-0170-6
UR  - conv_4118
ER  - 
@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Željko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Milanović, Zorana and Tomanović, Snežana",
year = "2017",
abstract = "Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts",
pages = "437-429",
number = "4",
volume = "72",
doi = "10.1007/s10493-017-0170-6",
url = "conv_4118"
}
Mihaljica, D., Marković, D., Radulović, Ž., Mulenga, A., Ćakić, S., Sukara, R., Milanović, Z.,& Tomanović, S.. (2017). Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology
Springer, Dordrecht., 72(4), 429-437.
https://doi.org/10.1007/s10493-017-0170-6
conv_4118
Mihaljica D, Marković D, Radulović Ž, Mulenga A, Ćakić S, Sukara R, Milanović Z, Tomanović S. Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology. 2017;72(4):429-437.
doi:10.1007/s10493-017-0170-6
conv_4118 .
Mihaljica, Darko, Marković, Dragana, Radulović, Željko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Milanović, Zorana, Tomanović, Snežana, "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts" in Experimental & Applied Acarology, 72, no. 4 (2017):429-437,
https://doi.org/10.1007/s10493-017-0170-6 .,
conv_4118 .

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB