Prenatal and Early Postnatal Diagnosis of Congenital Toxoplasmosis in a Setting With No Systematic Screening in Pregnancy
2016
Аутори
Štajner, TijanaBobić, Branko
Klun, Ivana
Nikolić, Aleksandra
Srbljanović, Jelena
Uzelac, Aleksandra
Rajnpreht, Irena
Đurković-Đaković, Olgica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
To determine the risk of congenital toxoplasmosis (CT) and provide early (pre-or postnatal) identification of cases of CT in the absence of systematic screening in pregnancy. In the presented cross-sectional study, serological criteria were used to date Toxoplasma gondii infection versus conception in 80 pregnant women with fetal abnormalities or referred to as suspected of acute infection, and in 16 women after delivery of symptomatic neonates. A combination of serological, molecular (qPCR), and biological (bioassay) methods was used for prenatal and/or postnatal diagnosis of CT. Most (77.5%) pregnant women were examined in advanced pregnancy. Of all the examined seropositive women (n = 90), infection could not be ruled out to have occurred during pregnancy in 93.3%, of which the majority (69%) was dated to the periconceptual period. CT was diagnosed in 25 cases, of which 17 prenatally and 8 postnatally. Molecular diagnosis proved superior, but the diagnosis of CT based on bioassay in... 7 instances and by Western blot in 2 neonates shows that other methods remain indispensable. In the absence of systematic screening in pregnancy, maternal infection is often diagnosed late, or even only when fetal/neonatal infection is suspected. In such situations, use of a complex algorithm involving a combination of serological, biological, and molecular methods allows for prenatal and/or early postnatal diagnosis of CT, but lacks the preventive capacity provided by early maternal treatment.
Извор:
Medicine, 2016, 95, 9Издавач:
- Lippincott Williams & Wilkins, Philadelphia
Финансирање / пројекти:
DOI: 10.1097/MD.0000000000002979
ISSN: 0025-7974
PubMed: 26945416
WoS: 000375208900054
Scopus: 2-s2.0-84962557744
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Štajner, Tijana AU - Bobić, Branko AU - Klun, Ivana AU - Nikolić, Aleksandra AU - Srbljanović, Jelena AU - Uzelac, Aleksandra AU - Rajnpreht, Irena AU - Đurković-Đaković, Olgica PY - 2016 UR - http://rimi.imi.bg.ac.rs/handle/123456789/719 AB - To determine the risk of congenital toxoplasmosis (CT) and provide early (pre-or postnatal) identification of cases of CT in the absence of systematic screening in pregnancy. In the presented cross-sectional study, serological criteria were used to date Toxoplasma gondii infection versus conception in 80 pregnant women with fetal abnormalities or referred to as suspected of acute infection, and in 16 women after delivery of symptomatic neonates. A combination of serological, molecular (qPCR), and biological (bioassay) methods was used for prenatal and/or postnatal diagnosis of CT. Most (77.5%) pregnant women were examined in advanced pregnancy. Of all the examined seropositive women (n = 90), infection could not be ruled out to have occurred during pregnancy in 93.3%, of which the majority (69%) was dated to the periconceptual period. CT was diagnosed in 25 cases, of which 17 prenatally and 8 postnatally. Molecular diagnosis proved superior, but the diagnosis of CT based on bioassay in 7 instances and by Western blot in 2 neonates shows that other methods remain indispensable. In the absence of systematic screening in pregnancy, maternal infection is often diagnosed late, or even only when fetal/neonatal infection is suspected. In such situations, use of a complex algorithm involving a combination of serological, biological, and molecular methods allows for prenatal and/or early postnatal diagnosis of CT, but lacks the preventive capacity provided by early maternal treatment. PB - Lippincott Williams & Wilkins, Philadelphia T2 - Medicine T1 - Prenatal and Early Postnatal Diagnosis of Congenital Toxoplasmosis in a Setting With No Systematic Screening in Pregnancy IS - 9 VL - 95 DO - 10.1097/MD.0000000000002979 ER -
@article{ author = "Štajner, Tijana and Bobić, Branko and Klun, Ivana and Nikolić, Aleksandra and Srbljanović, Jelena and Uzelac, Aleksandra and Rajnpreht, Irena and Đurković-Đaković, Olgica", year = "2016", abstract = "To determine the risk of congenital toxoplasmosis (CT) and provide early (pre-or postnatal) identification of cases of CT in the absence of systematic screening in pregnancy. In the presented cross-sectional study, serological criteria were used to date Toxoplasma gondii infection versus conception in 80 pregnant women with fetal abnormalities or referred to as suspected of acute infection, and in 16 women after delivery of symptomatic neonates. A combination of serological, molecular (qPCR), and biological (bioassay) methods was used for prenatal and/or postnatal diagnosis of CT. Most (77.5%) pregnant women were examined in advanced pregnancy. Of all the examined seropositive women (n = 90), infection could not be ruled out to have occurred during pregnancy in 93.3%, of which the majority (69%) was dated to the periconceptual period. CT was diagnosed in 25 cases, of which 17 prenatally and 8 postnatally. Molecular diagnosis proved superior, but the diagnosis of CT based on bioassay in 7 instances and by Western blot in 2 neonates shows that other methods remain indispensable. In the absence of systematic screening in pregnancy, maternal infection is often diagnosed late, or even only when fetal/neonatal infection is suspected. In such situations, use of a complex algorithm involving a combination of serological, biological, and molecular methods allows for prenatal and/or early postnatal diagnosis of CT, but lacks the preventive capacity provided by early maternal treatment.", publisher = "Lippincott Williams & Wilkins, Philadelphia", journal = "Medicine", title = "Prenatal and Early Postnatal Diagnosis of Congenital Toxoplasmosis in a Setting With No Systematic Screening in Pregnancy", number = "9", volume = "95", doi = "10.1097/MD.0000000000002979" }
Štajner, T., Bobić, B., Klun, I., Nikolić, A., Srbljanović, J., Uzelac, A., Rajnpreht, I.,& Đurković-Đaković, O.. (2016). Prenatal and Early Postnatal Diagnosis of Congenital Toxoplasmosis in a Setting With No Systematic Screening in Pregnancy. in Medicine Lippincott Williams & Wilkins, Philadelphia., 95(9). https://doi.org/10.1097/MD.0000000000002979
Štajner T, Bobić B, Klun I, Nikolić A, Srbljanović J, Uzelac A, Rajnpreht I, Đurković-Đaković O. Prenatal and Early Postnatal Diagnosis of Congenital Toxoplasmosis in a Setting With No Systematic Screening in Pregnancy. in Medicine. 2016;95(9). doi:10.1097/MD.0000000000002979 .
Štajner, Tijana, Bobić, Branko, Klun, Ivana, Nikolić, Aleksandra, Srbljanović, Jelena, Uzelac, Aleksandra, Rajnpreht, Irena, Đurković-Đaković, Olgica, "Prenatal and Early Postnatal Diagnosis of Congenital Toxoplasmosis in a Setting With No Systematic Screening in Pregnancy" in Medicine, 95, no. 9 (2016), https://doi.org/10.1097/MD.0000000000002979 . .