Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
2016
Autori
Bukara, KatarinaDrvenica, Ivana
Ilić, Vesna
Stančić, Ana
Misić, Danijela M.
Vasić, Borislav Z.
Gajić, Radoš
Vučetić, Dušan
Kiekens, Filip
Bugarski, Branko
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale dama...ges and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
Ključne reči:
Pharmaceutical biotechnology / Slaughterhouse blood / Erythrocyte membranes / Encapsulation / Gradual hypotonic hemolysis / Controlled drug deliveryIzvor:
Journal of Biotechnology, 2016, 240, 14-22Izdavač:
- Elsevier Science Bv, Amsterdam
Finansiranje / projekti:
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Fizika uređenih nanostruktura i novih materijala u fotonici (RS-MESTD-Basic Research (BR or ON)-171005)
DOI: 10.1016/j.jbiotec.2016.10.017
ISSN: 0168-1656
PubMed: 27773756
WoS: 000389116700002
Scopus: 2-s2.0-84992192143
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Bukara, Katarina AU - Drvenica, Ivana AU - Ilić, Vesna AU - Stančić, Ana AU - Misić, Danijela M. AU - Vasić, Borislav Z. AU - Gajić, Radoš AU - Vučetić, Dušan AU - Kiekens, Filip AU - Bugarski, Branko PY - 2016 UR - http://rimi.imi.bg.ac.rs/handle/123456789/689 AB - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Biotechnology T1 - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts EP - 22 SP - 14 VL - 240 DO - 10.1016/j.jbiotec.2016.10.017 ER -
@article{ author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna and Stančić, Ana and Misić, Danijela M. and Vasić, Borislav Z. and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko", year = "2016", abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Biotechnology", title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts", pages = "22-14", volume = "240", doi = "10.1016/j.jbiotec.2016.10.017" }
Bukara, K., Drvenica, I., Ilić, V., Stančić, A., Misić, D. M., Vasić, B. Z., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology Elsevier Science Bv, Amsterdam., 240, 14-22. https://doi.org/10.1016/j.jbiotec.2016.10.017
Bukara K, Drvenica I, Ilić V, Stančić A, Misić DM, Vasić BZ, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22. doi:10.1016/j.jbiotec.2016.10.017 .
Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna, Stančić, Ana, Misić, Danijela M., Vasić, Borislav Z., Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22, https://doi.org/10.1016/j.jbiotec.2016.10.017 . .