Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB...-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development.
TY - JOUR
AU - Krstić, Jelena
AU - Obradović, Hristina
AU - Jauković, Aleksandra
AU - Okić-Đorđević, Ivana
AU - Trivanović, Drenka
AU - Kukolj, Tamara
AU - Mojsilović, Slavko
AU - Ilić, Vesna
AU - Santibanez, Juan
AU - Bugarski, Diana
PY - 2015
UR - http://rimi.imi.bg.ac.rs/handle/123456789/666
AB - Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development.
PB - Elsevier Science Bv, Amsterdam
T2 - Biochimica et Biophysica Acta-Molecular Cell Research
T1 - Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration
EP - 444
IS - 2
SP - 431
VL - 1853
DO - 10.1016/j.bbamcr.2014.11.025
ER -
@article{
author = "Krstić, Jelena and Obradović, Hristina and Jauković, Aleksandra and Okić-Đorđević, Ivana and Trivanović, Drenka and Kukolj, Tamara and Mojsilović, Slavko and Ilić, Vesna and Santibanez, Juan and Bugarski, Diana",
year = "2015",
abstract = "Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta-Molecular Cell Research",
title = "Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration",
pages = "444-431",
number = "2",
volume = "1853",
doi = "10.1016/j.bbamcr.2014.11.025"
}
Krstić, J., Obradović, H., Jauković, A., Okić-Đorđević, I., Trivanović, D., Kukolj, T., Mojsilović, S., Ilić, V., Santibanez, J.,& Bugarski, D.. (2015). Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration. in Biochimica et Biophysica Acta-Molecular Cell Research
Elsevier Science Bv, Amsterdam., 1853(2), 431-444.
https://doi.org/10.1016/j.bbamcr.2014.11.025
conv_3428
Krstić J, Obradović H, Jauković A, Okić-Đorđević I, Trivanović D, Kukolj T, Mojsilović S, Ilić V, Santibanez J, Bugarski D. Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration. in Biochimica et Biophysica Acta-Molecular Cell Research. 2015;1853(2):431-444.
doi:10.1016/j.bbamcr.2014.11.025
conv_3428 .
