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Transforming growth factor-beta differently regulates urokinase type plasminogen activator and matrix metalloproteinase-9 expression in mouse macrophages; analysis of intracellular signal transduction
dc.creator | Mojsilović, Sonja | |
dc.creator | Santibanez, Juan F. | |
dc.date.accessioned | 2021-04-20T12:44:49Z | |
dc.date.available | 2021-04-20T12:44:49Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1065-6995 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/659 | |
dc.description.abstract | Transforming growth factor (TGF-) modulates capacity of macrophages to produce urokinase type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP9). uPA and MMP9 actively participate in extracellular matrix reorganization and influence macrophages chemotaxis and cell migration. Although, TGF- regulates uPA and MMP9 macrophages expression, the underlying intracellular signal mechanisms are not well elucidated so far. Here we have investigated the implication of TGF- signaling in the regulation of uPA and MMP9 expression in RAW 264.7 macrophages. The expression of uPA and MMP9 was assessed by zymography, Western blotting and RT-PCR. The involvement of Smad, MAPK or NFB signaling pathways was evaluated by using specific inhibitors. Our results indicated that TGF- simultaneously increased uPA and reduced MMP9 expression. The Smad3, ERK1,2, and JNK1,2 signaling pathways seem to be the main mechanisms that mediate TGF--induced uPA expression. Whereas TGF--reduced MMP9 expression appears to be regulated independently by JNK1,2 activation and by NFB signaling inhibition. Thus, our results suggested that, in murine macrophages, TGF- differentially regulates uPA and MMP9 expressions through different intracellular signaling mechanisms. In addition, presented data may help in understanding the role of TGF- in macrophages proteases regulation in inflammatory diseases. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175053/RS// | |
dc.rights | restrictedAccess | |
dc.source | Cell Biology International | |
dc.subject | macrophage | en |
dc.subject | matrix metalloproteinase-9 | en |
dc.subject | signal transduction | en |
dc.subject | transforming growth factor-beta | en |
dc.subject | urokinase type plasminogen activator | en |
dc.title | Transforming growth factor-beta differently regulates urokinase type plasminogen activator and matrix metalloproteinase-9 expression in mouse macrophages; analysis of intracellular signal transduction | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 628 | |
dc.citation.issue | 5 | |
dc.citation.other | 39(5): 619-628 | |
dc.citation.rank | M23 | |
dc.citation.spage | 619 | |
dc.citation.volume | 39 | |
dc.identifier.doi | 10.1002/cbin.10435 | |
dc.identifier.pmid | 25597879 | |
dc.identifier.scopus | 2-s2.0-84927788243 | |
dc.identifier.wos | 000353047300013 | |
dc.type.version | publishedVersion |