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Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress

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Authors
Vignjević, Sanja
Budeč, Mirela
Marković, Dragana
Đikić, Dragoslava
Mitrović, Olivera
Diklić, Miloš
Subotički, Tijana
Čokić, Vladan
Jovčić, Gordana
Article (Published version)
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Abstract
Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellulari...ty of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress.

Keywords:
psychological stress / erythroid progenitors / glucocorticoid receptor / corticosterone / spleen
Source:
Journal of Physiology & Pharmacology, 2015, 66, 1, 91-100
Publisher:
  • Polish Physiological Soc, Grzegorzecka
Funding / projects:
  • The pathogenetic mechanism in hematological malignancies (RS-175053)

ISSN: 0867-5910

PubMed: 25716969

WoS: 000350616600010

[ Google Scholar ]
14
Handle
https://hdl.handle.net/21.15107/rcub_rimi_653
URI
http://rimi.imi.bg.ac.rs/handle/123456789/653
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Vignjević, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Mitrović, Olivera
AU  - Diklić, Miloš
AU  - Subotički, Tijana
AU  - Čokić, Vladan
AU  - Jovčić, Gordana
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/653
AB  - Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellularity of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress.
PB  - Polish Physiological Soc, Grzegorzecka
T2  - Journal of Physiology & Pharmacology
T1  - Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress
EP  - 100
IS  - 1
SP  - 91
VL  - 66
UR  - conv_3446
ER  - 
@article{
author = "Vignjević, Sanja and Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Mitrović, Olivera and Diklić, Miloš and Subotički, Tijana and Čokić, Vladan and Jovčić, Gordana",
year = "2015",
abstract = "Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellularity of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress.",
publisher = "Polish Physiological Soc, Grzegorzecka",
journal = "Journal of Physiology & Pharmacology",
title = "Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress",
pages = "100-91",
number = "1",
volume = "66",
url = "conv_3446"
}
Vignjević, S., Budeč, M., Marković, D., Đikić, D., Mitrović, O., Diklić, M., Subotički, T., Čokić, V.,& Jovčić, G.. (2015). Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress. in Journal of Physiology & Pharmacology
Polish Physiological Soc, Grzegorzecka., 66(1), 91-100.
conv_3446
Vignjević S, Budeč M, Marković D, Đikić D, Mitrović O, Diklić M, Subotički T, Čokić V, Jovčić G. Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress. in Journal of Physiology & Pharmacology. 2015;66(1):91-100.
conv_3446 .
Vignjević, Sanja, Budeč, Mirela, Marković, Dragana, Đikić, Dragoslava, Mitrović, Olivera, Diklić, Miloš, Subotički, Tijana, Čokić, Vladan, Jovčić, Gordana, "Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress" in Journal of Physiology & Pharmacology, 66, no. 1 (2015):91-100,
conv_3446 .

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