Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress
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Vignjević, Sanja
Budeč, Mirela

Marković, Dragana

Đikić, Dragoslava
Mitrović, Olivera

Diklić, Miloš

Subotički, Tijana

Čokić, Vladan

Jovčić, Gordana
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Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellulari...ty of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress.
Keywords:
psychological stress / erythroid progenitors / glucocorticoid receptor / corticosterone / spleenSource:
Journal of Physiology & Pharmacology, 2015, 66, 1, 91-100Publisher:
- Polish Physiological Soc, Grzegorzecka
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Institut za medicinska istraživanjaTY - JOUR AU - Vignjević, Sanja AU - Budeč, Mirela AU - Marković, Dragana AU - Đikić, Dragoslava AU - Mitrović, Olivera AU - Diklić, Miloš AU - Subotički, Tijana AU - Čokić, Vladan AU - Jovčić, Gordana PY - 2015 UR - http://rimi.imi.bg.ac.rs/handle/123456789/653 AB - Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellularity of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress. PB - Polish Physiological Soc, Grzegorzecka T2 - Journal of Physiology & Pharmacology T1 - Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress EP - 100 IS - 1 SP - 91 VL - 66 UR - https://hdl.handle.net/21.15107/rcub_rimi_653 ER -
@article{ author = "Vignjević, Sanja and Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Mitrović, Olivera and Diklić, Miloš and Subotički, Tijana and Čokić, Vladan and Jovčić, Gordana", year = "2015", abstract = "Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellularity of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress.", publisher = "Polish Physiological Soc, Grzegorzecka", journal = "Journal of Physiology & Pharmacology", title = "Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress", pages = "100-91", number = "1", volume = "66", url = "https://hdl.handle.net/21.15107/rcub_rimi_653" }
Vignjević, S., Budeč, M., Marković, D., Đikić, D., Mitrović, O., Diklić, M., Subotički, T., Čokić, V.,& Jovčić, G.. (2015). Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress. in Journal of Physiology & Pharmacology Polish Physiological Soc, Grzegorzecka., 66(1), 91-100. https://hdl.handle.net/21.15107/rcub_rimi_653
Vignjević S, Budeč M, Marković D, Đikić D, Mitrović O, Diklić M, Subotički T, Čokić V, Jovčić G. Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress. in Journal of Physiology & Pharmacology. 2015;66(1):91-100. https://hdl.handle.net/21.15107/rcub_rimi_653 .
Vignjević, Sanja, Budeč, Mirela, Marković, Dragana, Đikić, Dragoslava, Mitrović, Olivera, Diklić, Miloš, Subotički, Tijana, Čokić, Vladan, Jovčić, Gordana, "Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress" in Journal of Physiology & Pharmacology, 66, no. 1 (2015):91-100, https://hdl.handle.net/21.15107/rcub_rimi_653 .