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Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia
dc.creator | Čokić, Vladan | |
dc.creator | Mojsilović, Slavko | |
dc.creator | Jauković, Aleksandra | |
dc.creator | Kraguljac-Kurtović, Nada | |
dc.creator | Mojsilović, Sonja | |
dc.creator | Sefer, Dijana | |
dc.creator | Mitrović-Ajtić, Olivera | |
dc.creator | Milošević, Violeta | |
dc.creator | Bogdanović, Andrija | |
dc.creator | Đikić, Dragoslava | |
dc.creator | Milenković, Pavle B. | |
dc.creator | Puri, Raj K. | |
dc.date.accessioned | 2021-04-20T12:43:01Z | |
dc.date.available | 2021-04-20T12:43:01Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1079-9796 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/631 | |
dc.description.abstract | Purpose: We compared the gene expression profile of peripheral blood CD34(+) cells and granulocytes in subjects with chronic myeloid leukemia (CML), with the accent on signaling pathways affected by BCR-ABL oncogene. Methods: The microarray analyses have been performed in circulating CD34(+) cells and granulocytes from peripheral blood of 7 subjects with CML and 7 healthy donors. All studied BCR-ABL positive CML patients were in chronic phase, with a mean value of 2012 +/- SD of CD34(+) cells/mu l in peripheral blood. Results: The gene expression profile was more prominent in CML CD34(+) cells (3553 genes) compared to granulocytes (2701 genes). The 41 and 39 genes were significantly upregulated in CML CD34(+) cells (HINT1, TXN, SERBP1) and granulocytes, respectively. BCR-ABL oncogene activated PI3K/AKT and MAPK signaling through significant upregulation of PTPN11, CDK4/6, and MYC and reduction of E2F1, KRAS, and NFKBIA gene expression in CD34(+) cells. Among genes linked to the inhibition of cellular proliferation by BCR-ABL inhibitor Imatinib, the FOS and STAT1 demonstrated significantly decreased expression in CML. Conclusion: The presence of BCR-ABL fusion gene doubled the expression quantity of genes involved in the regulation of cell cycle, proliferation and apoptosis of CD34(+) cells. These results determined the modified genes in PI3K/AKT and MAPK signaling of CML subjects. | en |
dc.publisher | Academic Press Inc Elsevier Science, San Diego | |
dc.relation | Intramural Research Program of Alan N. Schechter at the National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda [Z01 DK025016-33] | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175053/RS// | |
dc.relation | United States Department of Health & Human Services, National Institutes of Health (NIH) - USANIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) [Z01DK025016, Z01DK025016] | |
dc.rights | openAccess | |
dc.source | Blood Cells Molecules & Diseases | |
dc.subject | CD34(+) cells | en |
dc.subject | Granulocytes | en |
dc.subject | Chronic myeloid leukemia | en |
dc.subject | Microarray analysis | en |
dc.title | Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 381 | |
dc.citation.issue | 4 | |
dc.citation.other | 55(4): 373-381 | |
dc.citation.rank | M22 | |
dc.citation.spage | 373 | |
dc.citation.volume | 55 | |
dc.identifier.doi | 10.1016/j.bcmd.2015.08.002 | |
dc.identifier.fulltext | http://rimi.imi.bg.ac.rs/bitstream/id/486/628.pdf | |
dc.identifier.pmid | 26460262 | |
dc.identifier.scopus | 2-s2.0-84943534715 | |
dc.identifier.wos | 000363358100016 | |
dc.type.version | publishedVersion |