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dc.creatorČokić, Vladan
dc.creatorMojsilović, Slavko
dc.creatorJauković, Aleksandra
dc.creatorKraguljac-Kurtović, Nada
dc.creatorMojsilović, Sonja
dc.creatorSefer, Dijana
dc.creatorMitrović-Ajtić, Olivera
dc.creatorMilošević, Violeta
dc.creatorBogdanović, Andrija
dc.creatorĐikić, Dragoslava
dc.creatorMilenković, Pavle B.
dc.creatorPuri, Raj K.
dc.date.accessioned2021-04-20T12:43:01Z
dc.date.available2021-04-20T12:43:01Z
dc.date.issued2015
dc.identifier.issn1079-9796
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/631
dc.description.abstractPurpose: We compared the gene expression profile of peripheral blood CD34(+) cells and granulocytes in subjects with chronic myeloid leukemia (CML), with the accent on signaling pathways affected by BCR-ABL oncogene. Methods: The microarray analyses have been performed in circulating CD34(+) cells and granulocytes from peripheral blood of 7 subjects with CML and 7 healthy donors. All studied BCR-ABL positive CML patients were in chronic phase, with a mean value of 2012 +/- SD of CD34(+) cells/mu l in peripheral blood. Results: The gene expression profile was more prominent in CML CD34(+) cells (3553 genes) compared to granulocytes (2701 genes). The 41 and 39 genes were significantly upregulated in CML CD34(+) cells (HINT1, TXN, SERBP1) and granulocytes, respectively. BCR-ABL oncogene activated PI3K/AKT and MAPK signaling through significant upregulation of PTPN11, CDK4/6, and MYC and reduction of E2F1, KRAS, and NFKBIA gene expression in CD34(+) cells. Among genes linked to the inhibition of cellular proliferation by BCR-ABL inhibitor Imatinib, the FOS and STAT1 demonstrated significantly decreased expression in CML. Conclusion: The presence of BCR-ABL fusion gene doubled the expression quantity of genes involved in the regulation of cell cycle, proliferation and apoptosis of CD34(+) cells. These results determined the modified genes in PI3K/AKT and MAPK signaling of CML subjects.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationIntramural Research Program of Alan N. Schechter at the National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda [Z01 DK025016-33]
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175053/RS//
dc.relationUnited States Department of Health & Human Services, National Institutes of Health (NIH) - USANIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) [Z01DK025016, Z01DK025016]
dc.rightsopenAccess
dc.sourceBlood Cells Molecules & Diseases
dc.subjectCD34(+) cellsen
dc.subjectGranulocytesen
dc.subjectChronic myeloid leukemiaen
dc.subjectMicroarray analysisen
dc.titleGene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemiaen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage381
dc.citation.issue4
dc.citation.other55(4): 373-381
dc.citation.rankM22
dc.citation.spage373
dc.citation.volume55
dc.identifier.doi10.1016/j.bcmd.2015.08.002
dc.identifier.fulltexthttp://rimi.imi.bg.ac.rs/bitstream/id/486/628.pdf
dc.identifier.pmid26460262
dc.identifier.scopus2-s2.0-84943534715
dc.identifier.wos000363358100016
dc.type.versionpublishedVersion


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