Background: Phylogeographical studies allow precise genetic comparison of specimens, which were collected over large geographical ranges and belong to the same or closely related animal species. These methods have also been used to compare ticks of veterinary-medical importance. However, relevant data are missing in the case of ixodid ticks of bats, despite (1) the vast geographical range of both Ixodes vespertilionis and Ixodes simplex, and (2) the considerable uncertainty in their taxonomy, which is currently unresolvable by morphological clues. Methods: In the present study 21 ticks were selected from collections or were freshly removed from bats or cave walls in six European and four Asian countries. The DNA was extracted and PCRs were performed to amplify part of the cytochrome oxidase I (COI), 16S and 12S rDNA genes, followed by sequencing for identification and molecular-phylogenetic comparison. Results: No morphological differences were observed between Ixodes vespertilionis sp...ecimens from Spain and from other parts of Europe, but corresponding genotypes had only 94.6 % COI sequence identity. An I. vespertilionis specimen collected in Vietnam was different both morphologically and genetically (i.e. with only 84.1 % COI sequence identity in comparison with I. vespertilionis from Europe). Two ticks (collected in Vietnam and in Japan) formed a monophyletic clade and shared morphological features with I. ariadnae, recently described and hitherto only reported in Europe. In addition, two Asiatic specimens of I. simplex were shown to differ markedly from European genotypes of the same species. Phylogenetic relationships of ticks showed similar clustering patterns with those of their associated bat host species. Conclusions: Although all three ixodid bat tick species evaluated in the present study appear to be widespread in Eurasia, they exhibit pronounced genetic differences. Data of this study also reflect that I. vespertilionis may represent a species complex.
TY - JOUR
AU - Hornok, Sandor
AU - Estrada-Pena, Agustin
AU - Kontschan, Jeno
AU - Plantard, Olivier
AU - Kunz, Bernd
AU - Mihalca, Andrei D.
AU - Thabah, Adora
AU - Tomanović, Snežana
AU - Burazerović, Jelena
AU - Takacs, Nora
AU - Goerfoel, Tamas
AU - Estok, Peter
AU - Vuong, Tan Tu
AU - Szoke, Krisztina
AU - Fernandez de Mera, Isabel G.
AU - de la Fuente, Jose
AU - Takahashi, Mamoru
AU - Yamauchi, Takeo
AU - Takano, Ai
PY - 2015
UR - http://rimi.imi.bg.ac.rs/handle/123456789/629
AB - Background: Phylogeographical studies allow precise genetic comparison of specimens, which were collected over large geographical ranges and belong to the same or closely related animal species. These methods have also been used to compare ticks of veterinary-medical importance. However, relevant data are missing in the case of ixodid ticks of bats, despite (1) the vast geographical range of both Ixodes vespertilionis and Ixodes simplex, and (2) the considerable uncertainty in their taxonomy, which is currently unresolvable by morphological clues. Methods: In the present study 21 ticks were selected from collections or were freshly removed from bats or cave walls in six European and four Asian countries. The DNA was extracted and PCRs were performed to amplify part of the cytochrome oxidase I (COI), 16S and 12S rDNA genes, followed by sequencing for identification and molecular-phylogenetic comparison. Results: No morphological differences were observed between Ixodes vespertilionis specimens from Spain and from other parts of Europe, but corresponding genotypes had only 94.6 % COI sequence identity. An I. vespertilionis specimen collected in Vietnam was different both morphologically and genetically (i.e. with only 84.1 % COI sequence identity in comparison with I. vespertilionis from Europe). Two ticks (collected in Vietnam and in Japan) formed a monophyletic clade and shared morphological features with I. ariadnae, recently described and hitherto only reported in Europe. In addition, two Asiatic specimens of I. simplex were shown to differ markedly from European genotypes of the same species. Phylogenetic relationships of ticks showed similar clustering patterns with those of their associated bat host species. Conclusions: Although all three ixodid bat tick species evaluated in the present study appear to be widespread in Eurasia, they exhibit pronounced genetic differences. Data of this study also reflect that I. vespertilionis may represent a species complex.
PB - Biomed Central Ltd, London
T2 - Parasites & Vectors
T1 - High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia
VL - 8
DO - 10.1186/s13071-015-1056-2
UR - conv_3605
ER -
@article{
author = "Hornok, Sandor and Estrada-Pena, Agustin and Kontschan, Jeno and Plantard, Olivier and Kunz, Bernd and Mihalca, Andrei D. and Thabah, Adora and Tomanović, Snežana and Burazerović, Jelena and Takacs, Nora and Goerfoel, Tamas and Estok, Peter and Vuong, Tan Tu and Szoke, Krisztina and Fernandez de Mera, Isabel G. and de la Fuente, Jose and Takahashi, Mamoru and Yamauchi, Takeo and Takano, Ai",
year = "2015",
abstract = "Background: Phylogeographical studies allow precise genetic comparison of specimens, which were collected over large geographical ranges and belong to the same or closely related animal species. These methods have also been used to compare ticks of veterinary-medical importance. However, relevant data are missing in the case of ixodid ticks of bats, despite (1) the vast geographical range of both Ixodes vespertilionis and Ixodes simplex, and (2) the considerable uncertainty in their taxonomy, which is currently unresolvable by morphological clues. Methods: In the present study 21 ticks were selected from collections or were freshly removed from bats or cave walls in six European and four Asian countries. The DNA was extracted and PCRs were performed to amplify part of the cytochrome oxidase I (COI), 16S and 12S rDNA genes, followed by sequencing for identification and molecular-phylogenetic comparison. Results: No morphological differences were observed between Ixodes vespertilionis specimens from Spain and from other parts of Europe, but corresponding genotypes had only 94.6 % COI sequence identity. An I. vespertilionis specimen collected in Vietnam was different both morphologically and genetically (i.e. with only 84.1 % COI sequence identity in comparison with I. vespertilionis from Europe). Two ticks (collected in Vietnam and in Japan) formed a monophyletic clade and shared morphological features with I. ariadnae, recently described and hitherto only reported in Europe. In addition, two Asiatic specimens of I. simplex were shown to differ markedly from European genotypes of the same species. Phylogenetic relationships of ticks showed similar clustering patterns with those of their associated bat host species. Conclusions: Although all three ixodid bat tick species evaluated in the present study appear to be widespread in Eurasia, they exhibit pronounced genetic differences. Data of this study also reflect that I. vespertilionis may represent a species complex.",
publisher = "Biomed Central Ltd, London",
journal = "Parasites & Vectors",
title = "High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia",
volume = "8",
doi = "10.1186/s13071-015-1056-2",
url = "conv_3605"
}
Hornok, S., Estrada-Pena, A., Kontschan, J., Plantard, O., Kunz, B., Mihalca, A. D., Thabah, A., Tomanović, S., Burazerović, J., Takacs, N., Goerfoel, T., Estok, P., Vuong, T. T., Szoke, K., Fernandez de Mera, I. G., de la Fuente, J., Takahashi, M., Yamauchi, T.,& Takano, A.. (2015). High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia. in Parasites & Vectors
Biomed Central Ltd, London., 8.
https://doi.org/10.1186/s13071-015-1056-2
conv_3605
Hornok S, Estrada-Pena A, Kontschan J, Plantard O, Kunz B, Mihalca AD, Thabah A, Tomanović S, Burazerović J, Takacs N, Goerfoel T, Estok P, Vuong TT, Szoke K, Fernandez de Mera IG, de la Fuente J, Takahashi M, Yamauchi T, Takano A. High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia. in Parasites & Vectors. 2015;8.
doi:10.1186/s13071-015-1056-2
conv_3605 .
Hornok, Sandor, Estrada-Pena, Agustin, Kontschan, Jeno, Plantard, Olivier, Kunz, Bernd, Mihalca, Andrei D., Thabah, Adora, Tomanović, Snežana, Burazerović, Jelena, Takacs, Nora, Goerfoel, Tamas, Estok, Peter, Vuong, Tan Tu, Szoke, Krisztina, Fernandez de Mera, Isabel G., de la Fuente, Jose, Takahashi, Mamoru, Yamauchi, Takeo, Takano, Ai, "High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia" in Parasites & Vectors, 8 (2015),
https://doi.org/10.1186/s13071-015-1056-2 .,
conv_3605 .
