Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids
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Petrović, Snježana B.
Arsić, Aleksandra

Debeljak-Martačić, Jasmina

Durendic-Brenesel, Maja
Pilija, Vladimir
Milić, Nataša
Popović, Tamara B.

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The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains. Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20: 5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentag...e of oleic acid (18:1n-9) and estimated activity of Delta-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18: 0) accompanied with an increased estimated Delta-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Delta-6 desaturase activity. BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain.
Keywords:
buckwheat leaf and flower mixture / high-fat diet / brain phospholipids / fatty acidsSource:
Acta Veterinaria-Beograd, 2015, 65, 3, 390-403Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia (RS-41030)
- Functional Cereal Based Products for People with Metabolic Disorder (RS-31029)
DOI: 10.1515/acve-2015-0032
ISSN: 0567-8315
WoS: 000362123900008
Scopus: 2-s2.0-84943400753
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Institut za medicinska istraživanjaTY - JOUR AU - Petrović, Snježana B. AU - Arsić, Aleksandra AU - Debeljak-Martačić, Jasmina AU - Durendic-Brenesel, Maja AU - Pilija, Vladimir AU - Milić, Nataša AU - Popović, Tamara B. PY - 2015 UR - http://rimi.imi.bg.ac.rs/handle/123456789/627 AB - The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains. Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20: 5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentage of oleic acid (18:1n-9) and estimated activity of Delta-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18: 0) accompanied with an increased estimated Delta-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Delta-6 desaturase activity. BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids EP - 403 IS - 3 SP - 390 VL - 65 DO - 10.1515/acve-2015-0032 ER -
@article{ author = "Petrović, Snježana B. and Arsić, Aleksandra and Debeljak-Martačić, Jasmina and Durendic-Brenesel, Maja and Pilija, Vladimir and Milić, Nataša and Popović, Tamara B.", year = "2015", abstract = "The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains. Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20: 5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentage of oleic acid (18:1n-9) and estimated activity of Delta-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18: 0) accompanied with an increased estimated Delta-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Delta-6 desaturase activity. BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids", pages = "403-390", number = "3", volume = "65", doi = "10.1515/acve-2015-0032" }
Petrović, S. B., Arsić, A., Debeljak-Martačić, J., Durendic-Brenesel, M., Pilija, V., Milić, N.,& Popović, T. B.. (2015). Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(3), 390-403. https://doi.org/10.1515/acve-2015-0032
Petrović SB, Arsić A, Debeljak-Martačić J, Durendic-Brenesel M, Pilija V, Milić N, Popović TB. Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids. in Acta Veterinaria-Beograd. 2015;65(3):390-403. doi:10.1515/acve-2015-0032 .
Petrović, Snježana B., Arsić, Aleksandra, Debeljak-Martačić, Jasmina, Durendic-Brenesel, Maja, Pilija, Vladimir, Milić, Nataša, Popović, Tamara B., "Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids" in Acta Veterinaria-Beograd, 65, no. 3 (2015):390-403, https://doi.org/10.1515/acve-2015-0032 . .