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Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids

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Authors
Petrović, Snježana B.
Arsić, Aleksandra
Debeljak-Martačić, Jasmina
Durendic-Brenesel, Maja
Pilija, Vladimir
Milić, Nataša
Popović, Tamara B.
Article (Published version)
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Abstract
The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains. Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20: 5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentag...e of oleic acid (18:1n-9) and estimated activity of Delta-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18: 0) accompanied with an increased estimated Delta-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Delta-6 desaturase activity. BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain.

Keywords:
buckwheat leaf and flower mixture / high-fat diet / brain phospholipids / fatty acids
Source:
Acta Veterinaria-Beograd, 2015, 65, 3, 390-403
Publisher:
  • Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
  • Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia (RS-41030)
  • Functional Cereal Based Products for People with Metabolic Disorder (RS-31029)

DOI: 10.1515/acve-2015-0032

ISSN: 0567-8315

WoS: 000362123900008

Scopus: 2-s2.0-84943400753
[ Google Scholar ]
2
2
URI
http://rimi.imi.bg.ac.rs/handle/123456789/627
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Petrović, Snježana B.
AU  - Arsić, Aleksandra
AU  - Debeljak-Martačić, Jasmina
AU  - Durendic-Brenesel, Maja
AU  - Pilija, Vladimir
AU  - Milić, Nataša
AU  - Popović, Tamara B.
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/627
AB  - The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains. Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20: 5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentage of oleic acid (18:1n-9) and estimated activity of Delta-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18: 0) accompanied with an increased estimated Delta-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Delta-6 desaturase activity. BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids
EP  - 403
IS  - 3
SP  - 390
VL  - 65
DO  - 10.1515/acve-2015-0032
UR  - conv_3612
ER  - 
@article{
author = "Petrović, Snježana B. and Arsić, Aleksandra and Debeljak-Martačić, Jasmina and Durendic-Brenesel, Maja and Pilija, Vladimir and Milić, Nataša and Popović, Tamara B.",
year = "2015",
abstract = "The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains. Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20: 5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentage of oleic acid (18:1n-9) and estimated activity of Delta-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18: 0) accompanied with an increased estimated Delta-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Delta-6 desaturase activity. BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids",
pages = "403-390",
number = "3",
volume = "65",
doi = "10.1515/acve-2015-0032",
url = "conv_3612"
}
Petrović, S. B., Arsić, A., Debeljak-Martačić, J., Durendic-Brenesel, M., Pilija, V., Milić, N.,& Popović, T. B.. (2015). Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(3), 390-403.
https://doi.org/10.1515/acve-2015-0032
conv_3612
Petrović SB, Arsić A, Debeljak-Martačić J, Durendic-Brenesel M, Pilija V, Milić N, Popović TB. Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids. in Acta Veterinaria-Beograd. 2015;65(3):390-403.
doi:10.1515/acve-2015-0032
conv_3612 .
Petrović, Snježana B., Arsić, Aleksandra, Debeljak-Martačić, Jasmina, Durendic-Brenesel, Maja, Pilija, Vladimir, Milić, Nataša, Popović, Tamara B., "Effects of dietary supplementation with a mixture of buckwheat leaf and flower on fatty acid composition of rat brain phospholipids" in Acta Veterinaria-Beograd, 65, no. 3 (2015):390-403,
https://doi.org/10.1515/acve-2015-0032 .,
conv_3612 .

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