Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro
Само за регистроване кориснике
2014
Аутори
Trivanović, DrenkaNikolić, Srdjan
Krstić, Jelena
Jauković, Aleksandra
Mojsilović, Slavko
Ilić, Vesna
Okić Đorđević, Ivana
Santibanez, Juan F.
Jovčić, Gordana
Bugarski, Diana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), b...ut uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF- and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine.
Кључне речи:
adipose tissue / cancer / mesenchymal stem cellsИзвор:
Cell Biology International, 2014, 38, 2, 254-265Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
DOI: 10.1002/cbin.10198
ISSN: 1065-6995
PubMed: 24155046
WoS: 000329591100013
Scopus: 2-s2.0-84895908351
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Trivanović, Drenka AU - Nikolić, Srdjan AU - Krstić, Jelena AU - Jauković, Aleksandra AU - Mojsilović, Slavko AU - Ilić, Vesna AU - Okić Đorđević, Ivana AU - Santibanez, Juan F. AU - Jovčić, Gordana AU - Bugarski, Diana PY - 2014 UR - http://rimi.imi.bg.ac.rs/handle/123456789/602 AB - Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), but uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF- and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine. PB - Wiley, Hoboken T2 - Cell Biology International T1 - Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro EP - 265 IS - 2 SP - 254 VL - 38 DO - 10.1002/cbin.10198 ER -
@article{ author = "Trivanović, Drenka and Nikolić, Srdjan and Krstić, Jelena and Jauković, Aleksandra and Mojsilović, Slavko and Ilić, Vesna and Okić Đorđević, Ivana and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana", year = "2014", abstract = "Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), but uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF- and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine.", publisher = "Wiley, Hoboken", journal = "Cell Biology International", title = "Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro", pages = "265-254", number = "2", volume = "38", doi = "10.1002/cbin.10198" }
Trivanović, D., Nikolić, S., Krstić, J., Jauković, A., Mojsilović, S., Ilić, V., Okić Đorđević, I., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2014). Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro. in Cell Biology International Wiley, Hoboken., 38(2), 254-265. https://doi.org/10.1002/cbin.10198
Trivanović D, Nikolić S, Krstić J, Jauković A, Mojsilović S, Ilić V, Okić Đorđević I, Santibanez JF, Jovčić G, Bugarski D. Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro. in Cell Biology International. 2014;38(2):254-265. doi:10.1002/cbin.10198 .
Trivanović, Drenka, Nikolić, Srdjan, Krstić, Jelena, Jauković, Aleksandra, Mojsilović, Slavko, Ilić, Vesna, Okić Đorđević, Ivana, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro" in Cell Biology International, 38, no. 2 (2014):254-265, https://doi.org/10.1002/cbin.10198 . .