Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells ...should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
TY - JOUR
AU - Miletić, Maja
AU - Mojsilović, S.
AU - Okić-Đorđević, Ivana
AU - Kukolj, Tamara
AU - Jauković, Aleksandra
AU - Santibanez, Juan
AU - Jovčić, Gordana
AU - Bugarski, Diana
PY - 2014
UR - http://rimi.imi.bg.ac.rs/handle/123456789/599
AB - Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
PB - Srpsko biološko društvo, Beograd, i dr.
T2 - Archives of Biological Sciences
T1 - Mesenchymal stem cells isolated from human periodontal ligament
EP - 271
IS - 1
SP - 261
VL - 66
DO - 10.2298/ABS1401261M
ER -
@article{
author = "Miletić, Maja and Mojsilović, S. and Okić-Đorđević, Ivana and Kukolj, Tamara and Jauković, Aleksandra and Santibanez, Juan and Jovčić, Gordana and Bugarski, Diana",
year = "2014",
abstract = "Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Mesenchymal stem cells isolated from human periodontal ligament",
pages = "271-261",
number = "1",
volume = "66",
doi = "10.2298/ABS1401261M"
}
Miletić, M., Mojsilović, S., Okić-Đorđević, I., Kukolj, T., Jauković, A., Santibanez, J., Jovčić, G.,& Bugarski, D.. (2014). Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(1), 261-271.
https://doi.org/10.2298/ABS1401261M
Miletić M, Mojsilović S, Okić-Đorđević I, Kukolj T, Jauković A, Santibanez J, Jovčić G, Bugarski D. Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences. 2014;66(1):261-271.
doi:10.2298/ABS1401261M .
Miletić, Maja, Mojsilović, S., Okić-Đorđević, Ivana, Kukolj, Tamara, Jauković, Aleksandra, Santibanez, Juan, Jovčić, Gordana, Bugarski, Diana, "Mesenchymal stem cells isolated from human periodontal ligament" in Archives of Biological Sciences, 66, no. 1 (2014):261-271,
https://doi.org/10.2298/ABS1401261M . .
