The Effect of Paraprotein on Platelet Aggregation

2014
Authors
Đunić, IrenaElezović, Ivo
Ilić, Vesna

Milošević-Jovčić, Nadežda
Bila, Jelena
Suvajdžić-Vuković, Nada
Antić, Darko

Vidović, Ana
Tomin, Dragica
Article (Published version)

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Background Some patients with paraproteinemia have platelet aggregation disorders and the aim of this study was to examine disturbance of platelet aggregation in healthy blood donors by isolated paraprotein in vitro. Methods Using Rivanol, paraprotein was separated from the serum of ten patients with paraproteinemia, who had decreased platelet aggregation with several inducers. Platelet aggregation in ten healthy donors was measured with and without addition of the isolated induced paraprotein. The test was repeated with added human immunoglobulins for intravenous use. Results Average of maximal levels of platelet aggregation has been significantly decreased in plasma rich in platelets (PRP) of healthy donors after addition of paraprotein when inducers are used: adenosine diphosphate (ADP) (P = 0.007), collagen (COL) (P = 0.008), ristocetin (RIS) (P = 0.001), and epinephrine (EPI) (P = 0.002). Average of latent time of platelet aggregation was significantly prolonged in healthy donors ...after addition of paraprotein with inducers: COL (P = 0.008), RIS (P = 0.008) and EPI (P = 0.006) while addition of human immunoglobulins caused no change in platelet aggregation. In comparison, when human immunoglobulins were added, maximal platelet aggregation and latent time did not change significantly. Paraprotein isolated from patients with paraproteinamia, who had decrease platelet aggregation, had significantly decreased platelet aggregation when added to PRP of healthy donors, in vitro. Conclusion Platelet aggregation was not significantly changed was confirmed with addition of human immunoglobulins.
Keywords:
paraproteinemia / role of paraprotein / platelet aggregationSource:
Journal of Clinical Laboratory Analysis, 2014, 28, 2, 141-146Publisher:
- John Wiley & Sons Inc, Hoboken
DOI: 10.1002/jcla.21658
ISSN: 0887-8013
PubMed: 24395751
WoS: 000332840500011
Scopus: 2-s2.0-84896120163
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Institut za medicinska istraživanjaTY - JOUR AU - Đunić, Irena AU - Elezović, Ivo AU - Ilić, Vesna AU - Milošević-Jovčić, Nadežda AU - Bila, Jelena AU - Suvajdžić-Vuković, Nada AU - Antić, Darko AU - Vidović, Ana AU - Tomin, Dragica PY - 2014 UR - http://rimi.imi.bg.ac.rs/handle/123456789/566 AB - Background Some patients with paraproteinemia have platelet aggregation disorders and the aim of this study was to examine disturbance of platelet aggregation in healthy blood donors by isolated paraprotein in vitro. Methods Using Rivanol, paraprotein was separated from the serum of ten patients with paraproteinemia, who had decreased platelet aggregation with several inducers. Platelet aggregation in ten healthy donors was measured with and without addition of the isolated induced paraprotein. The test was repeated with added human immunoglobulins for intravenous use. Results Average of maximal levels of platelet aggregation has been significantly decreased in plasma rich in platelets (PRP) of healthy donors after addition of paraprotein when inducers are used: adenosine diphosphate (ADP) (P = 0.007), collagen (COL) (P = 0.008), ristocetin (RIS) (P = 0.001), and epinephrine (EPI) (P = 0.002). Average of latent time of platelet aggregation was significantly prolonged in healthy donors after addition of paraprotein with inducers: COL (P = 0.008), RIS (P = 0.008) and EPI (P = 0.006) while addition of human immunoglobulins caused no change in platelet aggregation. In comparison, when human immunoglobulins were added, maximal platelet aggregation and latent time did not change significantly. Paraprotein isolated from patients with paraproteinamia, who had decrease platelet aggregation, had significantly decreased platelet aggregation when added to PRP of healthy donors, in vitro. Conclusion Platelet aggregation was not significantly changed was confirmed with addition of human immunoglobulins. PB - John Wiley & Sons Inc, Hoboken T2 - Journal of Clinical Laboratory Analysis T1 - The Effect of Paraprotein on Platelet Aggregation EP - 146 IS - 2 SP - 141 VL - 28 DO - 10.1002/jcla.21658 UR - conv_3196 ER -
@article{ author = "Đunić, Irena and Elezović, Ivo and Ilić, Vesna and Milošević-Jovčić, Nadežda and Bila, Jelena and Suvajdžić-Vuković, Nada and Antić, Darko and Vidović, Ana and Tomin, Dragica", year = "2014", abstract = "Background Some patients with paraproteinemia have platelet aggregation disorders and the aim of this study was to examine disturbance of platelet aggregation in healthy blood donors by isolated paraprotein in vitro. Methods Using Rivanol, paraprotein was separated from the serum of ten patients with paraproteinemia, who had decreased platelet aggregation with several inducers. Platelet aggregation in ten healthy donors was measured with and without addition of the isolated induced paraprotein. The test was repeated with added human immunoglobulins for intravenous use. Results Average of maximal levels of platelet aggregation has been significantly decreased in plasma rich in platelets (PRP) of healthy donors after addition of paraprotein when inducers are used: adenosine diphosphate (ADP) (P = 0.007), collagen (COL) (P = 0.008), ristocetin (RIS) (P = 0.001), and epinephrine (EPI) (P = 0.002). Average of latent time of platelet aggregation was significantly prolonged in healthy donors after addition of paraprotein with inducers: COL (P = 0.008), RIS (P = 0.008) and EPI (P = 0.006) while addition of human immunoglobulins caused no change in platelet aggregation. In comparison, when human immunoglobulins were added, maximal platelet aggregation and latent time did not change significantly. Paraprotein isolated from patients with paraproteinamia, who had decrease platelet aggregation, had significantly decreased platelet aggregation when added to PRP of healthy donors, in vitro. Conclusion Platelet aggregation was not significantly changed was confirmed with addition of human immunoglobulins.", publisher = "John Wiley & Sons Inc, Hoboken", journal = "Journal of Clinical Laboratory Analysis", title = "The Effect of Paraprotein on Platelet Aggregation", pages = "146-141", number = "2", volume = "28", doi = "10.1002/jcla.21658", url = "conv_3196" }
Đunić, I., Elezović, I., Ilić, V., Milošević-Jovčić, N., Bila, J., Suvajdžić-Vuković, N., Antić, D., Vidović, A.,& Tomin, D.. (2014). The Effect of Paraprotein on Platelet Aggregation. in Journal of Clinical Laboratory Analysis John Wiley & Sons Inc, Hoboken., 28(2), 141-146. https://doi.org/10.1002/jcla.21658 conv_3196
Đunić I, Elezović I, Ilić V, Milošević-Jovčić N, Bila J, Suvajdžić-Vuković N, Antić D, Vidović A, Tomin D. The Effect of Paraprotein on Platelet Aggregation. in Journal of Clinical Laboratory Analysis. 2014;28(2):141-146. doi:10.1002/jcla.21658 conv_3196 .
Đunić, Irena, Elezović, Ivo, Ilić, Vesna, Milošević-Jovčić, Nadežda, Bila, Jelena, Suvajdžić-Vuković, Nada, Antić, Darko, Vidović, Ana, Tomin, Dragica, "The Effect of Paraprotein on Platelet Aggregation" in Journal of Clinical Laboratory Analysis, 28, no. 2 (2014):141-146, https://doi.org/10.1002/jcla.21658 ., conv_3196 .