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Clinical significance of molecular methods in the diagnosis of imported malaria in returning travelers in Serbia
dc.creator | Dakić, Zorica | |
dc.creator | Ivović, Vladimir | |
dc.creator | Pavlović, Milorad | |
dc.creator | Lavadinović, Lidija | |
dc.creator | Marković, Marija | |
dc.creator | Đurković-Đaković, Olgica | |
dc.date.accessioned | 2021-04-20T12:37:05Z | |
dc.date.available | 2021-04-20T12:37:05Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1201-9712 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/536 | |
dc.description.abstract | Objectives: The goal of this study was to assess the clinical significance of conventional and PCR-based molecular diagnosis in patients with imported malaria in Serbia. Methods: Giemsa microscopy, the rapid diagnostic test, and quantitative real-time PCR (qPCR) were used to detect Plasmodium species in 109 whole-blood samples from patients after their return from malaria endemic areas, including those clinically suspected for malaria (n = 97) and healthy travelers (n = 12) examined as part of epidemiological surveillance. Results: A total of 45 patients were diagnosed with malaria: 42 (93.3%) by microscopy and three (6.7%) additional ones by qPCR. The agreement between the results of species-specific qPCR and microscopy was 73.3%; it was as high as 90.6% for Plasmodium falciparum infections. Follow-up analysis demonstrated persistence of Plasmodium sp DNA for a mean 6 days after the disappearance of parasitemia on microscopy. Conclusions: Due to its sensitivity and specificity, qPCR is a helpful method complementary to microscopy, particularly in cases of low parasitemia. In addition, it is superior to microscopy for species identification. | en |
dc.publisher | Elsevier Sci Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41019/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | International Journal of Infectious Diseases | |
dc.subject | Malaria | en |
dc.subject | Plasmodium species | en |
dc.subject | Diagnosis | en |
dc.subject | Quantitative real-time PCR | en |
dc.subject | Microscopy | en |
dc.subject | Parasitemia | en |
dc.title | Clinical significance of molecular methods in the diagnosis of imported malaria in returning travelers in Serbia | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 30 | |
dc.citation.other | 29: 24-30 | |
dc.citation.rank | M23 | |
dc.citation.spage | 24 | |
dc.citation.volume | 29 | |
dc.identifier.doi | 10.1016/j.ijid.2014.08.013 | |
dc.identifier.fulltext | http://rimi.imi.bg.ac.rs/bitstream/id/407/533.pdf | |
dc.identifier.pmid | 25312981 | |
dc.identifier.scopus | 2-s2.0-84910097459 | |
dc.identifier.wos | 000348023600005 | |
dc.type.version | publishedVersion |